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2007, Vol.34, No.1 Previous Issue    Next Issue

果树

  • Morphological Development of Ovule-like Organ on the Leaf and VariationCharacteristics of Ginkgo biloba var. epiphylla Mak. (Ohatsuki)
  • LI Shi-mei;LIBao-jin;XING Shi-yan;WANG Fang
  • Acta Horticulturae Sinica. 2007, 34(1): 1-6.
  • Abstract ( 1693 ) HTML ( 1372 ) PDF (829KB) ( 1372 )    
  • Many features of Ginkgo biloba var. epiphylla Mak. , the one which was firstly found at Yiyuan
    County of Shandong Province in China, were continually observed and the comparative morphology research
    was conducted, including the variation characteristics, the morphological development of ovule-like organ on
    the leaf blade and veins of leaf. The results of analysis showed that the development of the ovule-like organ on
    the leaf blade is comp rised of initial stage, formation stage, expansion stage and mature stage. There were one
    to eight ovule2like organs on each leaf, but only one developed normally. The leaves were divided into five
    types, i. e. multilobed type, nonlobed type, bilobed type, the leaf with ovule-like organ and deformation
    leaf, leaf color into two types, i. e. green and variegated types. Compared with normal ovules, ovule-like
    organ on the leaf blade disp layed variation in insertion, heterochrony and hysteresis in development of ovule-
    like organ on the leaf blade, which was reported for the first time. The ovule2like organ lagged behind the nor-
    mal ovule for fifteen days at initial stage, moreover no pollination drop was found on the ovule2like organ. The
    ovule2like organ on the leaf blade was divided into five types according to the insertion on the leaf, i. e. uni-
    lobed solitary type, unilobed clustered type, bilobed clustered type, multilobed solitary type and adsperse
    type. The vein of normal leaf could be separated into four types according to anastomoses, i. e. closed type,
    W type, V +W type and double V +W type. And that of the phyllosporous leaf was classified into three
    types, i. e. uni-arcuate, biarcuate and mutli-arcuate type. The variation characteristics and the phylogeny of
    Ginkgo biloba var. epiphylla Mak. were discussed in the paper.
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  • Studies on the Vessel Elements of Secondary Xylem in Manilkara zapota
  • CHEN Shu-si
  • Acta Horticulturae Sinica. 2007, 34(1): 7-10.
  • Abstract ( 1537 ) HTML ( 1433 ) PDF (543KB) ( 1433 )    
  • The vessel elements of secondary xylem in Manilkara zapota were observed by bio-microscope
    image analysis system and micrography. There were various patterns of vessel elements in M. zapota and most
    of the vessel elements had tail. Perforation p lates could be divided into two types: 1. Both ends of the vessel
    element were simp le perforation p late; 2. One end had simple perforation, the other had two simp le perfora
    tions. And there were a series of transitional patterns between the two types. Three percent of vessel elements
    of secondary xylem in the p lant had inclusions. Intervascular p itting was alternate p itting. There were mixed
    type p itting, gash-like p itting and perforation between vessel elements and ray cells.
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  • Structural and Developmental Characteristics of Floral Nectaries ofDimocarpus longan
  • NING Xi-ping;WU Hong;LUO Shi;LAI Yong-chao
  • Acta Horticulturae Sinica. 2007, 34(1): 11-16.
  • Abstract ( 2117 ) HTML ( 1985 ) PDF (1155KB) ( 1985 )    
  • The structure and development of floral nectaries of Dimocarpus longan were studied with
    scanning electron microscopy ( SEM ) , paraffin section, thin resin sections and transmission electron
    microscopy (TEM). The floral nectaries ofmale and female flowers of Dim ocarpus longan were located on the
    surface of floral recep tacle, which formed a convex disc-like nectary. The nectarywas located at the outer part
    of the pistil and stamen and at the inner part of corolla and calyx. The male and female flower nectaries,
    composed of epidermis, nectariferous tissue and vascular tissue containing only phloem elements, belong to
    typical structural nectaries. The epidermis of floral nectaries was covered with numerous epidermal hairs
    consisting of single cellwith thickened wall and cuticle. In addition, some small stomata were observed at the
    top surface of nectaries. There were a lot of granulose phenolic compounds within most ep idermis cells. Two
    types of cells could be distinguished in nectariferous tissue, one was smaller and had densely stained cyto
    p lasm, anotherwas larger and contained phenolic compounds. The former consisted ofmore cells than the lat
    er. The nectaries had well-developed vascular tissues, which derived from the branches of the vascular bundle
    of floral recep tacle, then they terminated nearby ep idermis. Normally the nectariesps p rimordia ofmale and fe
    male flower ofD. longan were found to initiate in surface of recep tacle after other floral organswere differenti
    ated. During the development of floral nectaries, especially before and after the nectar secretion, the vacuole
    volume ofmost of nectariferous tissue cells changed regularly, which imply that they may be involved in the
    p rocess of nectar synthesis and secretion. Some phenolic compounds in specialized cells of nectariferous tissue were disintegrating during nectar secretion, so besides its p rotective function in floral nectaries, the phenolic
    compounds were likely to take part in synthesis of nectar.
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  • Frequency Distribution of Several Biolog ical Characters in Different ApricotEco-geographical Groups Native to China
  • HE Tian-ming;CHEN Xue-sen;ZHANG Da-hai;XU Lin;LIU Ning;GAO Jiang-sheng;XU Zheng
  • Acta Horticulturae Sinica. 2007, 34(1): 17-22.
  • Abstract ( 1925 ) HTML ( 1341 ) PDF (213KB) ( 1341 )    
  • In order to exploit the evolution of genes of apricot native to China, the frequency distribution
    of several biological characters of apricot in different eco-geographical groups (North China, Central Asia,
    Dzhungar - Zailij) native to China were analyzed in the study. In general, self-incompatibility was a common
    character for the three eco-geographical groups with averages less than 2.0% and the percentages of self
    incompatible cultivarsmore than 90%. Therefore, a few self-compatible germp lasmswere found, too. Higher
    sterile flower rate of 40% was observed in all eco-geographical groups with a higher coefficient variance of
    50%. For a certain cultivar or form, the trait was relatively stable. Glabrous-fruited cultivars were only ob
    served in Central Asian eco-geographical group with a higher percentage of 76.6%. In other two group s, the cultivars or forms were all identified as rough skin-fruit. The largest fruit size (with an average of 51.4 g) was
    a predominate trait in North China group compared with those of Central Asian group (23.2 g) and Dzhungar
    - Zailij group (8.2 g). In the three groups, the rate of freestone-fruited cultivars or forms was remarkably
    higher than those of clingstone. The frequencies of freestone were different among the group swith the highest
    frequency of 94.6% in Dzhungar - Zailij group and the lowest frequency of 58.8% in North China group. The
    highest value of the total soluble solid wasmeasured in Central Asia group (18.5% ). The total soluble solid
    was greatly affected by environmental factors indicating itwas an classical quantitative trait. For cultivars from
    North China, the valuesmeasured from Shandong (13.1%) and Xinjiang (16.1% ) were different very sig
    nificantly ( F = 42.361, P < 0.01). The highest rate of sweet kernel (93.1% ) was observed in the Central
    Asian eco-geographical group compared to Ili wild population ( 0.9% ) and North China group ( 44.4% ).
    The effect of artificial selection and natural selection on the origin and evolution of the characterswas also dis
    cussed.
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  • Phylogenetic Analysis of Stone Fruits Such as Peach, Plum, Apricot, Mumeand Cherry Based on ITS Sequences
  • LIU Yan-ling;XU Li-ming;CHENG Zhong-ping
  • Acta Horticulturae Sinica. 2007, 34(1): 23-28.
  • Abstract ( 2100 ) HTML ( 2065 ) PDF (427KB) ( 2065 )    
  • The internal transcribed spacer ( ITS) regions of nuclear ribosomal DNA from 8 species of
    Amygdalus L. were sequenced, and analyzed togetherwith other ITS sequences ( from GenBank) of 18 species
    representing genera Cerasus Mill. , Armeniaca Mill. , and Prunus L. Taking Padus racem osa (Lam. ) Gilib.
    and Padus buergeriana (Miq. ) Y et Ku as outgroup s, phylogenetic analysis of ITS sequences was made by
    using PAUP410b4a software. Resusts indicated that 1) The consensus tree could be divided into two main
    clades, CerasusMill. and the remaining genera, supported by bootstrap values of 68% and 100%, respective
    ly. 2) Species of CerasusMill. formed a basal group (Clade Ⅰ). Prunus L. , Arm eniaca Mill. and Amygda
    lus
    L. formed a monophyletic group (CladeⅡ) with bootstrap value of 100% , which imp lied that they had
    close relationship swith each other and probably had a common origin. CladeⅡwas divided into two subclades. One was Amygdalus L. , and the other was Prunus L. and Armeniaca Mill. Bootstrap values for these two
    subcladeswere 100% and 54%, respectively. The evolutional and taxonomic relationship s of stone fruitswere
    discussed based on the phylogenetic analysis of ITS sequences, morphological characters and geographical dis
    tribution.
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  • Interspecific Hybridization of Prunus persica with P. armeniaca and P.salicina Using Embryo Rescue
  • LIU Wen;CHEN Xue-sen;LIU Guan-jun;WU Yan
  • Acta Horticulturae Sinica. 2007, 34(1): 29-34.
  • Abstract ( 2109 ) HTML ( 1822 ) PDF (498KB) ( 1822 )    
  • Some interspecific hybrids were obtained after hybridization of Prunus persica with treated
    pollens from P. armeniaca and P. salicina, then the hybrid embryos were successfully rescued, and a molecu
    lar identification wasmade in part of the hybrids. The results showed that the hybrid embryo abortion started
    from the 7 th or 8 th week after pollination in cross when used the Yuhualu or Zhonghua Shoutao as the female
    parent. The cultivarswith different durations for fruit development and the ages of hybrid embryos have great
    effects on the rate of embryo germination and growth. At the same age (when the PF value was around 0.5) ,
    the late peach cultivar Zhonghua Shoutao acquired more than 50% of embryo germination rate, but the early
    peach cultivar Yuhualu gave only 6.7% of it. Treatment of male parent pollen in the electrostatic field at an
    app rop riate dosage imp roved the efficiency of bud inducement and multip lication, but treatment with 60Coγ
    ray reduced the efficiency and p roduced weak buds. For rescuing the hybrid embryos, it is concluded that the
    medium 1 /2MS + 6-BA 4 mg·L-1 + IBA 0.5 mg·L-1 can increase the embryo germination rate up to
    90% , the MS + 6-BA 1.0 mg·L-1 + IBA 1.0 mg·L-1 is the bestmedium for bud induction and multip li
    cation, and the MS + IAA 0.5 mg·L-1 + NAA 0.2 mg·L-1 is the best rootingmedium. SSR identification
    proves that the hybrids obtained by the embryo rescuing system are true ones.
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  • Photosynthetic Characteristics of Cherry Grown in Pot
  • QIN Si-jun;Lü De-guo;LIU Guo-cheng;LI Zuo-xuan
  • Acta Horticulturae Sinica. 2007, 34(1): 35-38.
  • Abstract ( 1412 ) HTML ( 1309 ) PDF (454KB) ( 1309 )    
  • The photosynthetic characteristics of Cerasus sachalinensis Kom. and C. pseudocerasus G.
    Don. ‘Daqingye’ grown in field and in pot were studied by gas exchange. The results showed that the net
    photosynthetic rate ( Pn) , photosynthetic capacity, carboxylation efficiency (CE) , apparent quantum yield
    (AQY) and maximum regeneration rate of RuBP of cherry grown in field were higher than those grown in pot.
    Pn, transp iration ( Tr) ratio and stomata conductance (Gs) of cherry grown in field were higher than those
    grown in pot, but the intercellular CO2 concentration (Ci) was lower than that in pot during daytime. The
    physiologicalmechanism in photosynthesis of cherry grown in field differed from the potted p lants.
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  • Simple Sequence Repeat Analysis on Genetic Assessment of Chinese RedSkinned Sand Pear Cultivars
  • ZHANG Dong;SHU Qun;TENG Yuan-wen;QIU Ming-hua;BAO Lu;HU Hong-ju
  • Acta Horticulturae Sinica. 2007, 34(1): 47-52.
  • Abstract ( 1723 ) HTML ( 1905 ) PDF (440KB) ( 1905 )    
  • A total of 29 sand pear germp lasm (mainly red skinned) native to southwest of China were
    subjected to simp le sequence repeat ( SSR) analysis. Six pairs of SSR p rimers (BGA35, KU10, BGT23b,
    NH004a, NH011b and NH015a) could generate a large number of alleles. The p rimer NH004a, which p ro
    duced the highest allele numbers and the most effective alleles, and high heterozygosity and Shannon informa
    tion index, showed the best identification power. Using six pairs of SSR p rimers, all accessions or types could
    be distinguished excep t for three pairs of cultivars, which might belong to the synonymy cases or bud mutants.
    Twenty nine pear accessions could be divided into fourmajor group s obviously based on the UPGMA cluster a
    nalysis. Group Ⅰ consisted of cultivars all native to Yunnan p rovince, including two green and nine red
    skinned sand pears, inferring a near genetic relationship between the red and green skinned pears. In groups
    Ⅱand Ⅲ, the red skinned pears from Huili County of Sichuan Province and from Yunnan Province mingled to
    gether, which might suggest cultivarmovement from Yuannan to Sichuan. In group Ⅳ, not onlywere Xiangsu
    li and Zaiyangli from Huili County of Sichuan Province, Midu Xiangsuli and Changshui Huobali native to Yun
    nan Province not closely clustered, but also were far from other groups. Huobali pear types distributed in Yun
    nan and Sichuan p rovincesmight have different origins. The results above indicates large genetic diversity of
    germp lasm resources of red skinned sand pear cultivars in China
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  • Detection of Apple chlorotic leaf spot virus in Pear by in situ RT-PCR
  • NIU Jian-xin;ZHOU Min-sheng;MA Bing-gang;ZHAO Ying;LIU Hong
  • Acta Horticulturae Sinica. 2007, 34(1): 53-58.
  • Abstract ( 1624 ) HTML ( 1222 ) PDF (638KB) ( 1222 )    
  • The detection technique of Apple chlorotic leaf spot virus (ACLSV) in pear plant by IS-RT-
    PCR with Digoxigenin labels for leaf paraffin slice samp les and shoot tip s frost slice samples was established
    in this paper. Korla pear leaves infected by ACLSV and virus-free seedling leaves of Pyrus betulaefolia Bge
    as control were used as leaves materials in the tests. The IS-PCR parameters including concentration of
    dNTPs, RNasin, Taq DNA polymerase, Mg2+ and p rimers, effect ofAMV reverse transcrip tase and comple
    ment primer on cDNA synthesis, annealing temperature and cycle number were studied systematically. The
    results showed that the stain signal strength increased accompanied with an increase of RNasin amountwhen
    its concentration reached over 0.2 U·μL-1. Certain amount of cDNA would not be generated until the con
    centration of dNTPs reached 1.0 mmol·L -1 in reaction solution. Reverse transcrip tion could be carried out
    when the concentration of AMV reverse transcrip tase was between 0.3 U·μL-1 and 0.5 U·μL-1, and the
    quantity of cDNA p roduction imp roved with the increase of the concentration ofAMV. The reverse transcrip
    tion could not be carried out until the concentration of antisense p rimer reached 0.9μmol·L -1 , and the a
    mount of cDNA would increase alongwith the increase of primers concentration. The suitable annealing tem
    perature for in situ amp lification of cDNA was 56℃. The minimum amp lification cycle number was 20 times. The stain signal was weak when primer concentration was less than 018 μmol·L - 1 and the signal
    color was heavy blue as the concentration of Taq DNA polymerase was over 20 U·mL -1; In situ PCR could
    be performed whenMg 2+ concentration was at 1.5 mmol·L-1. An op timized detection system of ACLSV
    by IS-RT-PCR has been established and used to detect virus in Korla pear orchard, and the stable detection
    results have been obtained.
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  • Obtainment of Transgenic ‘Xueqing’ Pear Plants with a Synthetic Cry1AcGene Mediated by Agrobacterium tumefaciens
  • TANG Shao-hu;SUN Min;LIAO Zhi-hua;ZHOU Qi-gui;LIDao-gao
  • Acta Horticulturae Sinica. 2007, 34(1): 59-62.
  • Abstract ( 1648 ) HTML ( 1174 ) PDF (255KB) ( 1174 )    
  • By using calli from leaves of Xueqing pear ( Pyrus sp. ) as explants, the Cry1Ac gene regula
    ted by CaMV35S promoter was introduced into Xueqing pear through Ag robacterium-mediated transformation.
    After co-culturing 698 calli and 231 Kanr buds, respectively, with A. tum efaciens strain LBA4404 harboring a
    plasmid pBX203 for 3 days and transferred to the selective medium containing 50 mg·L-1 kanamycin for 30
    days at a 15-day interval, 34.24% kanamycin2resistant (Kanr ) buds ( adventitious shoots) regenerated on MS
    + 5 mg·L-1 6-BA + 0.1 mg·L-1 IAA and 15.58% of Kanr buds rooted on 1/2 MS + 2 mg·L-1 IBA + 0.5
    mg·L-1 6-BA + 500 mg·L-1 AC. A total of 32 plants were obtained, but only 4 p lants were confirmed by
    PCR and Southern-blot analysis that the Cry1Ac gene was transferred and integrated into the genome of
    Xueqing pear.
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  • Investigation and Studies on Classif ication of W ild Fragaria spp. Distributedin Changbai Mountains
  • DAI Han-ping;LEI Jia-jun;DENG Ming-qin
  • Acta Horticulturae Sinica. 2007, 34(1): 63-66.
  • Abstract ( 1631 ) HTML ( 1429 ) PDF (251KB) ( 1429 )    
  • The wild strawberry germplasm resources are widely distributed in Changbai Mountains. The
    strawberry gemplasm resources in Changbai Mountains were investigated and totally 20 wild strawberry geno
    types had been collected in recent 10 years. Their taxonomic characters were observed and described, and
    their classification was studied by comparison. These wild strawberries are classified into 3 species according to
    the study, which are dip loid Fragaria m andschurica Staudt, tetrap loid F. orientalis Lozinsk. and F. corymbo
    sa
    Lozinsk. F. orientalis var. concolor distributed in Changbai Mountains, which was regarded as the variety
    of F. orientalis Lozinsk. before, was denied according to the p resent study and itwas identified as F. corym
    bosa
    Lozinsk. This is the first report that F. corymbosa Lozinsk. is distributed in Changbai Mountains
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  • Response of Photosynthesis to Some Env ironmental Factors of High-bushBlueberry Cultivars
  • SUN Shan;LI Peng-min;LIU Qing-zhong;GAO Hui-yuan
  • Acta Horticulturae Sinica. 2007, 34(1): 67-70.
  • Abstract ( 1765 ) HTML ( 2004 ) PDF (365KB) ( 2004 )    
  • The photosynthetic induction and response of photosynthesis to environmental factors were
    investigated in 4 high-bush blueberry cultivars. The results showed that the time of photosynthetic induction
    was quite long ( 46 - 75 min) , the photosynthetic induction was the fastest in Reveille, whereas it was the
    slowest in Sunrise. The CO2 compensation points were rather high ( 89 - 121 μmol ·mol-1 ) in the 4
    cultivars, indicating that all of the 4 cultivars are typ ical C3 p lants. Compared to southern high2bush blueber
    ries, the northern high2bush blueberries Sunrise and Jersey had higher photosynthetic ability, carboxylation
    efficiency, the maximal photochemical efficiency of PS Ⅱ and saturation light intensity. In addition, the
    Sunrise had the lowest light compensation point and characteristics of shade tolerant p lant. However, the
    op timum photosynthetic temperature was lower and the range of itwas narrower (23 - 25℃) , indicating that
    these cultivars were not suitable to grow in southern region with high temperature. The southern high-bush
    blueberries Reveille, as compared with northern high2bush blueberries, had a wider range (23 - 35℃) of op
    timum photosynthetic temperature, which extended its range of introduction and cultivation.
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  • Flowering and Pollen Fertility of an Interspecific CitrusHexaploid Somatic Hybrid
  • GUO Wen-wu;CHEN Chun-li;DENG Yu-yang;DENG Xiu-xin
  • Acta Horticulturae Sinica. 2007, 34(1): 71-74.
  • Abstract ( 1597 ) HTML ( 1152 ) PDF (401KB) ( 1152 )    
  • Interspecific hexap loid somatic hybrid between J incheng orange and HR
    (Hamlin orange + Rough lemon ) regenerated via electrofusion flowered seven years after
    transp lantation in greenhouse. Flow cytometry analysis further confirmed its true hexap loid
    nature. Its pollen stainability and germinabilitywas 40.8% and 3.7% , respectively. In 2006,
    the hexalpoid plant flowered again and its pollen stainability was 20.3%. Since there is no
    natural citrus hexaploid plant, this is the first report of fertile hexap loid somatic hybrid plant in
    Citrus.
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  • Chromosome Localization of 45S rDNA in Different Ploidy Citrus grandis byFluorescence in situ Hybridization
  • XIANG Su-qiong;WANG Wei-xing;LIANG Guo-lu
  • Acta Horticulturae Sinica. 2007, 34(1): 75-80.
  • Abstract ( 1701 ) HTML ( 1547 ) PDF (381KB) ( 1547 )    
  • Chromosome localization of 45S rDNA was studied bymeans of fluorescence in situ hybridiza
    tion ( FISH) on metaphase chromosomes of different p loidy Citrus grandis. NOR loci of different materials
    showed obvious polymorphism and heterozygosity. There were four 45S rDNA loci in diploid, while there were
    ten loci in tetrap loid t1 and eight loci in tetrap loid t2. Chromosomal location of rDNA loci included five types,
    i1e. the telomeric region of the short arm, centromere to the short arm region, the centromeric region, centro
    mere to the long arm region, the telomeric region of the long arm. Different materials had incomp lete same
    rDNA loci types. The number of loci was not the same in different situations, the most were types of centro
    mere to short arm region and centromeric region, next was the telomeric region of the short arm. There was a
    variation in DNA among the homologous tetrap loids with the corresponding dip loid. Meanwhile, the citrus
    chromosome slides used for F ISH and rDNA loci situation on chromosome, genetic variation analysis among the
    homologous tetrap loids and dip loids, repeated sequence of chromosome in situ hybridization (CISH) for chro
    mosome identification and species evolvementwere discussed.
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  • Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana
  • WEI Yue-rong;YANG Hu;HUANG Bing-zhi;HUANG Xia;HUANG Xue-lin;QIU Ji-shui;XU Lin-bing
  • Acta Horticulturae Sinica. 2007, 34(1): 81-86.
  • Abstract ( 2608 ) HTML ( 2689 ) PDF (592KB) ( 2689 )    
  • Establishment of a stable embryogenic cell suspension ( ECS) is a prerequisite for biotechno
    logical breeding of banana. Although many important p rogress has been made, but app lication of embryogenic
    cell suspension for genetic imp rovement ofM usa is limited because of low induction percentage of embryogeno
    ic callus, low conversion frequence of p lant regeneration from the somatic embryos. Therefore, an op timal pro
    tocol for embryogenic cell suspensions is still required to establish. In this study the effects of Picloram, ABA
    and TDZ on somatic embryogenesis of banana from youngmale flower of Musa acuminata cv. Mas (AA) were
    investigated. Results showed that the induction percentage of embryogenic calli reached 15.6% when 2, 4-D
    in the callus induction medium was substituted by 8.28 μmol/L picloram. However, the addition ofABA into
    somatic embryo development medium inhibited somatic embryogenesis from embryogenic cell suspension and
    resulted in callus formation from somatic embryos. Treatmentwith TDZ at concentration of 0.2 μmol/L resul
    ted in increase of the percentage of germination of somatic embryos from 17.28% to 72.77% and the plant
    conversion ratio from 16.49% to 67.05% , respectively.
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  • Analysis on the Genetic Relationship of Some Mango (Mangifera indica L. )Germplasms by ISSRMarkers
  • WANG J ia-bao;WANG Ling-xia;DU Zhong-jun;LE IXin-tao;CHEN Ye-yuan;XU B i-yu
  • Acta Horticulturae Sinica. 2007, 34(1): 87-92.
  • Abstract ( 1616 ) HTML ( 1480 ) PDF (460KB) ( 1480 )    
  • The genetic relationship s of 38 mango (Mangifera indica L. ) accessions were examined by
    ISSR markers. Of the 60 ISSR p rimers screened, only 10 p rimers gave rep roducible, polymorphic DNA ampli
    fication patternswith a total 79 of amplified bands. Their percentage of polymorphic band was 86.7%. By
    banding patterns obtained from these 10 primers, each accession could be distinguished from the others excep t
    either Hongjinlong or Guifei, since the two accessions had the same amplified band patterns. Genetic distances
    among the tested accessions ranged from 0 to 0.682 with an average of 0.393. Five group s were formed by
    UPGMA clustering and the classification results were discussed in this paper.
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蔬菜

  • Genetic Effect of Sterility of the Dominant Genic Male Sterile Materile(DGMS79-399-3) in Cabbage ( Brassica oleracea var. capitata L. )
  • YAN Hui-ling;FANG Zhi-yuan;LIU Yu-mei;WANG Yong-jian;YANG Li-mei;ZHUANG Mu;ZHANG Yang-yong;SUN Pei-tian
  • Acta Horticulturae Sinica. 2007, 34(1): 93-93098.
  • Abstract ( 1557 ) HTML ( 1307 ) PDF (315KB) ( 1307 )    
  • Genetic effect of the dominant genic male sterile material DGMS79-399-3 in cabbage (Brassi
    ca oleracea
    var. capitata L. ) was studied with two different genetic analysismodels. The principle of genetics
    was studied with the method of GriffingⅡ, and the result showed that the variance of additive effect in the total
    genetic variance was only 21189% , but the dominant variance was 78.11%. The value of the broad
    heritability was 97.22% , but the value of narrow heritability was only 21.23%. The result of comparison of
    combining ability, in the five male sterile parents, the highest general combining ability of P1 from 523-16 was
    3.17, but the highest special combining ability from the combination of 606-7 ×102 was 10.42. The p rinciple
    of geneticswas studied with joint analysis of six generations and the result indicated that the best fitted genetic
    model affecting fertile characteristics of the dominant genetic male sterility in cabbage was one major additive
    dominant gene p lus additive2dominant2ep istatic polygenes (D model) , the heritability of major gene was
    80.03% - 94.12% , and the heritability of polygeneswas 0.14% - 10.94%. The major dominant gene had
    an important effect, however, the additive effect ofmajor gene and the polygenes effect should be also valued.
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  • Mapping and Analysis of QTL Related to Leaf Color in Chinese Cabbage( Brassica rapa L. ssp. pekinensis)
  • XU Dong-hui;SUN Ri-fei;ZHANG Yan-guo;YUAN Yu-xiang;;KANG Jun-gen;WU Jian;ZHANG Hui;SONG Xiao-fei;LI Xiao-nan;SONG Yi-mo;WANG Xiao-wu
  • Acta Horticulturae Sinica. 2007, 34(1): 99-104.
  • Abstract ( 1944 ) HTML ( 1303 ) PDF (717KB) ( 1303 )    
  • A Chinese cabbage (Brassica rapa L. ssp. pekinensis) double-hap loid (DH) population with a
    reported AFLP genetic map was emp loyed to map quantitative trait loci (QTL ) related to leaf color by using
    MAPQTL 410 package. Tristimulus colorimeter, leafy p igment determination and ocularmeasurementwere used
    to measure the traits related to leaf color. A total of 18 QTL were detected on 7 linkage group s, 9, 8 and 1 QTL
    were detected by 3 methods, respectively. These QTL individually exp lained 5.5% to 15.6% of the phenotyp ic
    variation, and there were unequal gene effects on the traits related to leaf color. These mapped QTL could be
    used to develop a marker assisted selection program for leaf color in Brassica rapa vegetable varieties
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  • Cloning and Sequence Analysis of Chinese Cabbage Chitinase Gene CHB4
  • GAN De-fang;ZHU Su-wen;FAN Jun;CHENG Bei-jiu
  • Acta Horticulturae Sinica. 2007, 34(1): 105-110.
  • Abstract ( 1962 ) HTML ( 1463 ) PDF (1108KB) ( 1463 )    
  • Based on the conserved sequence of the chitinase of crucifers, about 1 kb DNA fragment and
    5′2 end fragment (150 bp) were amp lified by PCR from genomic DNA of Chinese cabbage. Experimentswere
    performed on 72day2old Chinese cabbage seedlings after germination with salicylic acid of different concentra
    tions. Total RNA was p repared from the p lantwith higher chitinase activity, and 3′2 end fragmentwas amp li
    fied by 3′RACE. Comparing Chinese cabbage chitinase sequence cloned in this experimentwith that reported
    in GenBank composed of 1 094 bp nucleotide of Brassica napus, the experiment showed that the chitinase se
    quence of Chinese cabbage is 1 517 bp in size with one intron of 508 bp (DDBJ accession number:
    AB257452) , and shares as high as 99% identitywith B. napus and over 50% with other high p lants in cod
    ing region.
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  • Isolated Microspore Culture in Brassica campes tris ssp. chinensis
  • GENG Jian-feng;;HOU Xi-lin;ZHANG Xiao-wei;JIANG Wu-sheng;YUAN Yu-xiang;HAN Yong-ping;YAO Qiu-ju;CHENG Yan;LI Ying
  • Acta Horticulturae Sinica. 2007, 34(1): 111-116.
  • Abstract ( 1556 ) HTML ( 2117 ) PDF (504KB) ( 2117 )    
  • Several factors affecting isolated microspore culture of non-heading Chinese cabbage
    (Brassica campestris ssp. chinensis) were studied in experiments. The results from mono2factor experiments
    showed that there were significant differences in the frequency ofmicrospore-induced embryos among different
    genotypes. In the low temperature treatment, no significant differences were found during 0 - 5 days, while
    the frequencies were obviously lower when the period was longer than five days. There were no significant
    differences in high temperature when the incubation time was within 12 - 60 h, but the frequencies of
    microspore-induced embryos were much lower over this duration. The addition of auxins (NAA ) and the
    cytokinins ( 6-BA) in NLN medium showed no significant effect on the frequencies of microspore-induced
    embryos, but when the concentration was excessive, the frequencies decreased. The presence and
    concentration of activated charcoal had a great influence on the frequency ofmicrospore-induced embryos. Mo
    reover, four-factor analysis of genotype, auxin, cytokinins and activated charcoal concentrations indicated that
    there were significant differences among different genotypes and activated charcoal concentrations,
    respectively, and also significant differences among these two interactions, while other interactions remained
    non-significant.
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  • Cloning, Characterization and Expression of a Polygalacturonase GeneBcMF6 from Chinese Cabbage-pak-choi( Brassica campestris L. ssp. chinensis Makino)
  • ZHANG Qiang;HUANG Li;CAO J ia-shu
  • Acta Horticulturae Sinica. 2007, 34(1): 117-124.
  • Abstract ( 1745 ) HTML ( 1474 ) PDF (1691KB) ( 1474 )    
  • On the basis of one cDNA-AFLP differential fragment isolated from the fertile Bline of
    Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) nuclear recessive sterile A /B line
    (Bajh97201A /B) , the full length DNA and cDNA of BcMF6 gene encoding pollen2specific polygalacturonase
    were cloned by rapid amplification of cDNA ends (RACE). The gene consisted of 1 194 bp encoding a
    protein of 397 amino acids and was interrup ted by three introns of 81 bp, 95 bp and 127 bp in length.
    Sequence analysis revealed that it has three N-glycosylation sites, four protein kinase Cphosphorylation site,
    five casein kinaseⅡphosphorylation site, ten N-myristoylation sites and one polygalacturonase active position
    (227RVTCGPGHGIS1 IGS240 ). And the first 22 amino acids of the predicted BcMF6 protein form a N-terminal
    hydrophobic domain which disp layed the p roperties of a signal peptide and four parallel beta-helix repeats
    followed behind. Four domains which were highly conserved in all p lant and fungal PGs was p resent in
    BcMF6. Phylogenetic analysis showed that BcMF6 falls into the category of clade2C, which included PG
    related to pollen. These results showed that BcMF6 acts as pollen-specific polygalacturonase. Furthermore,
    the exp ression using RT-PCR discovered that BcMF6 was exclusively exp ressed in middle and big flower bud,opened flower and slow pod of fertile line (wild type) , which showed BcMF6 closely related with the develop
    ment of flower.
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  • Effects of Silenc ing BcMF3 by RNAi on Pollen Development of FloweringChinese Cabbage
  • LIU Le-cheng;;XIANG Xun;CAO J ia-shu
  • Acta Horticulturae Sinica. 2007, 34(1): 125-130.
  • Abstract ( 1566 ) HTML ( 1413 ) PDF (795KB) ( 1413 )    
  • In an effort to study the molecular mechanism of genic male sterility in plants, BcMF3 gene
    that encodes a pectin methylesterase ( PME) was isolated from the fertile B line of Chinese cabbage-pak-choi
    (Brassica campestris ssp. chinensis var. communis, syn. B. rapa ssp. chinensis var. communis) previously.
    In the present paper, primerswere designed based on the cDNA sequence of B cM F3, and a 458 bp fragment
    and a 538 bp fragmentwere amp lified from the cDNA of flower buds of Chinese cabbage-pak-choi. These two
    fragmentswere introduced into binary vector pB I121 in antisense orientation and sense orientation respectively,
    and the generated RNA interference (RNAi) vector was then mobilized into Agrobacterium tum efaciens strain
    LBA4404. The A. tum efaciens harboring the BcMF3 fragment was transformed to flowering Chinese cabbage
    (B. campestris ssp. chinensis var. parachinensis) via tissue culture. The pollen grains from app roximately
    50% of RNAi plants exhibited abnormal shapes, and only 32.3% of pollens from RNAi plants germinated nor
    mally. The PME activity of the anther from RNAi plants with abnormal pollens decreased by 13.5%. These
    results showed that functional interruption of BcMF3 by RNAi resulted in pollen abortion in flowering Chinese
    cabbage, which p robably caused by the decrease of PME activity. The study suggests that the product of
    BcMF3 gene plays an important role during pollen development in Cruciferae crop s such as flowering Chinese
    cabbage and Chinese cabbage-pak-choi.
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  • Histological Stud ies of Somatic Embryogenesis in Eruca sativa Mill.
  • ZHANG Tao;
  • Acta Horticulturae Sinica. 2007, 34(1): 131-134.
  • Abstract ( 1657 ) HTML ( 1345 ) PDF (489KB) ( 1345 )    
  • Somatic embryos were induced from cotyledon explants cultured on Murashige and Skoog
    (MS) medium supplemented with 1.0 mg·L-1 2, 4-D after two weeks of culture. Embryogenic callus prolif
    erated p rofusely on MS medium containing 0.2 mg·L-1 2, 4-D. Somatic embryosmatured on N6 medium and
    developed into p lantlets by culturing the mature somatic embryos on 1/2 MS medium containing 0.1 mg·L-1
    indole-3-butyric acid. Section and microscop ic observation of different developmental stages revealed that so
    matic embryogenesis was similar to that of the zygotic embryo. It originated from a single embryogenic cell,
    which had bigger nuclei, thick cytop lasm, clear nucleoli and denser order. The embryogenic cell first divided
    into two-celled stage proembryo, then three- or four-celled stage proembryo. Three- or four-celled stage pro
    embryo developed into multicellular proembryo, then developed into mature somatic embryo.
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  • Construction of a Molecular Genetic Map for Melon ( Cucumis melo L. )Based on SRAP
  • WANG Jian-she;YAO Jian-chun;;LIU Ling;WANG Yong-jian;LI Wei
  • Acta Horticulturae Sinica. 2007, 34(1): 135-140.
  • Abstract ( 1706 ) HTML ( 1696 ) PDF (578KB) ( 1696 )    
  • A molecularmap formelon (Cucumis melo L. ) was constructed with SRAP markers using a
    population consisting of 114 F2 individuals derived from the cross of 4G21 (C. melo var. chinensis) and
    3A832 (C. melo var. saccherinus). Twenty-nine primer pairswere used and produced 187 polymorphic loci.
    The map consists of 12 linkage groups, which include 152 genetic markers, and covers 2 077.1 cM with an
    average genetic distance of 13.67 cM. Every linkage group has 6 - 32 genetic markers, average genetic dis
    tance is 9.72 - 19.19 cM, and the length of linkage group is 85.3 - 496.1 cM.
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  • Comparison and Analysis of C Gene from Different Pepper Accessions
  • WANG Yan;;ZHANG Bao-xi;ZHANG Yan-guo;ZHANG Guo-yu;WANGLi-hao;XU You-ming;WANG Xiao-wu
  • Acta Horticulturae Sinica. 2007, 34(1): 143-146.
  • Abstract ( 1565 ) HTML ( 1245 ) PDF (427KB) ( 1245 )    
  • Deletion of 689 bp in 5′- terminal of the C gene locus caused an absence of pungency in sweet
    pepper after sequencing and alignment. A C gene fragment from non-pungent oxhorn pepper 0538 was amp li
    fied, which does not have the deletion, but a mutation from G to A at 40 bp in the coding region. Thismuta
    tion led to the change of an amino acid from hydrophobic aspartic acid to hydrophilic asparaginate in signal
    pep tide, which might cause the loss of the function of the C gene and finally the pungency. Using the same
    primer pair, Catf2 gene from all used accessions was amp lified, which shares high similarity about 88.9%
    with the C gene.
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  • Study on Osmotic Adjustment Mechanism of Toma to Salt ToleranceEnhanced by Arbuscular Mycorrhizal Fungi
  • HE Zhong-qun.;HE Chao-xing;ZHANG Zhi-bin;ZOU Zhi-rong;Wang Huai-song;TUNDE Takacs
  • Acta Horticulturae Sinica. 2007, 34(1): 147-152.
  • Abstract ( 1575 ) HTML ( 1394 ) PDF (814KB) ( 1394 )    
  • The effects of arbuscular mycorrhizal fungi (AMF) on p lant growth and osmotic adjustment
    matter content of pot cultured tomato under NaCl stress ( 0.5% and 1% ) were studied. The results showed
    that AMF-inoculation significantly increased tomato p lant growth, promoted the accumulation of soluble sugar
    in leaves and roots, increased soluble p rotein in leaves and proline content in roots under salt stress, so AMF
    enhanced salt tolerance of tomato plants. The accumulation of soluble sugar, soluble p rotein and root p roline
    induced by AMF played an important role in osmotic adjustmentmechanism of enhanced salt tolerance in my
    corrhizal tomato.

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观赏植物

  • Relationship Between Carbohydrate Allocation and Photosynthesis DuringFlorescence and Flower Senescence Period in Tree Peony
  • FENG Ya-nan;ZHENG Guo-sheng;;WANG Zong-zheng;GAO Hui-yuan
  • Acta Horticulturae Sinica. 2007, 34(1): 153-156.
  • Abstract ( 1700 ) HTML ( 1447 ) PDF (390KB) ( 1447 )    
  • Gas exchange, chlorophyll fluorescence and bio2analysis were used to study the influence of
    carbohydrate allocation on net photosynthetic rate in leaves of tree peony ( Paeonia suffruticosa ‘Rou Furong’)
    brancheswith and without flower. The results showed that before flower senescence, the net photosynthetic
    rate in leaves on brancheswith flower ( FL) was lower than that in leaves on brancheswithout flower (NFL).
    Whereas the total soluble sugar content was higher in the FL than that in the NFL. Compared with that at
    17∶00 on the p revious day, after a night consump tion, the total soluble sugar content in the NFL decreased
    obviously at 8 ∶00 a1m. while that in the FL hardly changed. These results indicate that there were other
    sources to supp ly soluble sugar in FL besides photosynthesis during the florescence period. After flower senes
    cence, differences in net photosynthetic rate and total soluble sugar content between the NFL and the FL dis
    appeared gradually, and the total soluble sugar content in both the FL and the NFL was lower at 8: 00 a. m.
    than that at 17∶00 on the p revious day, which suggest that the other sourcesmentioned above did notwork to
    supp ly carbohydrates to the leaves any more. It is speculated that thismetabolic pathway might be correlated
    with flower growth.
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  • Effects of Drought Stress on Photosynthesis of Gerbera Modified by PSAG12-ipt
  • LAI Qi-xian;;BAO Zhi-yi;ZHU Zhu-jun;MAO Bi-zeng;QIAN Qiong-qiu;HE Yong
  • Acta Horticulturae Sinica. 2007, 34(1): 157-162.
  • Abstract ( 1963 ) HTML ( 1390 ) PDF (756KB) ( 1390 )    
  • The chlorophyll fluorescence parameters ( actual quantum yield of PSⅡ photochemistry, ΦPS
    Ⅱ; quantum efficiency of open PSⅡ reaction centers, Fv’/Fm’; photochemical quenching coefficient, qP;
    electron transport rate, ETR) and photosynthesis parameters ( net photosynthetic rate, Pn; stomatal conduct
    ance, Gs) of the gerbera modified by PSAG12-ipt were investigated under drought stress. The results showed that
    ΦPSⅡ, Fv’/Fm’, qP, ETR, Pn and Gs decreased after drought stress and gradually increased after wate
    ring recovery. However, these decreases caused by drought stress were lower in the modified gerbera than in
    the wild type. The modified gerbera showed a higher tolerance to drought stress. The fraction of absorbed light
    in photochemistry ( P) decreased under drought stress, but being higher in the modified gerbera than in the
    wild type. The fraction of thermal dissipation (D) was increased under drought stress. However, the fraction
    of excess energy (E) was not changed during drought stress and recovery
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  • Effects of Drought and Flooding Stress on Photosynthetic Characteristics ofPhotinia frasery in Summer and Autumn
  • CAO Jing;JIANGWei-bing;WENG Mang-ling;JIANG Wu
  • Acta Horticulturae Sinica. 2007, 34(1): 163-172.
  • Abstract ( 1765 ) HTML ( 1435 ) PDF (600KB) ( 1435 )    
  • By controlling the soilwater content, 1-year-old potted cutting plants of Photinia frasery as the
    materials, the effects of drought and flooding stress on the changes of malondialdehyde (MDA) content, the
    membrane permeability, p igment content aswell as photosynthetic and physiological characters in summer and
    autumn were studied. The results showed that MDA content, membrane permeability and anthocyanin (Ant)
    content were distinctly increased, while the content of chlorophyll, the index values of net photosynthesis rate
    ( Pn) , water use efficiency (WUE) , apparent quantum yield (AQY) and carboxylation efficiency (CE) were
    decreased. Meanwhile the available light intensity range was shortened with the declining of light saturation
    point (LSP) and rising of light compensation point (LCP) under drought and flooding stress in summer and
    autumn. On the other hand, the chlorophyll (Chl. ) content of control in summer was lower than that in au
    tumn, but the values of Pn and CE in summerwere higher than these in autumn. In addition, the membrane
    injury of Photinia frasery under drought and flooding stress in summerwasworse than autumn, the degradation
    of Chl. in summerwas lower than autumn, the carotinoid (Car. ) content increased in summer and decreased
    in autumn, the decrease of LSP and increase of LCP in summerwere lower than autumn, but the decrease of
    Pn under drought stress in summer was lower than autumn, and the decrease of Pn under flooding stress in summerwas higher than autumn; moreover, flooding in summer had much more influence on membrane injury
    and Pn, WUE, AQY, CE than drought stress, the decrease of Pn in initial stage was due to stomatal limita
    tion, and then nonstomatal limitation dominated. The gray relational grade analysis showed that the relation
    ship among climate factors and Pn ( from high to low) were temperature of atmosphere (Ta) , CO2 concentra
    tion and photon fluxdensity ( PFD). While drought in autumn had much more influence on membrane injury
    and Pn, WUE, AQY, CE than flooding stress, the decrease of Pn wasmainly decided by nonstomatal limita
    tion; The gray relational grade analysis showed that the retational factors to Pn ( from high to low) were Vapor
    p ressure (Vp) , PFD, and then CO2 concentration.
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  • Effects of Shading on Net Photosynthetic Rate and Chlorophyll FluorescenceParameters of Leaf in David Maple (Acer davidii Franch. )
  • JIAO Li-li;LU Bing-she;ZHOU Ru-jiu;BAI Zhi-ying;LIANG Hai-yong;ZHEN Hong-wei
  • Acta Horticulturae Sinica. 2007, 34(1): 173-178.
  • Abstract ( 1743 ) HTML ( 1477 ) PDF (664KB) ( 1477 )    
  • The shoot growth and the pigment content, net photosynthetic rate, transp iration rate and
    chlorophyll fluorescence of leaf under different shading treatments (0% , 15% , 35% and 50% ) were investi
    gated in david maple seeding. The results showed that the p igment content, net photosynthetic rate, minimal
    fluorescence ( Fo ) , conversion efficiency of p rimary light energy ( Fv/Fm ) of PS Ⅱ and photochemical
    quenching coefficient ( qP) were enhanced, but the non-photochemical quenching coefficient ( qN) was de
    creased as shading increased. The diurnal variation curve of net photosynthetic rate had two peaks under 0%
    and 15% shading treatments, while there was only one peak under 35% and 50% shading treatments. The di
    urnal variations of Fv/Fm showed increase-decrease-increase curve under 0% and 15% shading treatments,
    and kep tstable in 35% and 50% shading treatments. It is concluded that 50% shading suitable for the growth
    of david maple seedlings.
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  • Studies on Juvenility and Limited Inductive Photoperiod of Petunia hybrida‘Fanta sy’
  • HU Hui-rong;HU Xiao-long;DI Wen-wei;BAO Man-zhu
  • Acta Horticulturae Sinica. 2007, 34(1): 179-182.
  • Abstract ( 1804 ) HTML ( 1158 ) PDF (299KB) ( 1158 )    
  • Juvenility and limited inductive photoperiod (LIP) of Petunia hybrida ‘Fantasy’were exam
    ined by transferring plants between short days ( SD) and long days (LD). It is concluded that the end of the
    juvenile phase of petunia is about 62leaf stage, and the LIP of petunia is 3 weeks. LIP inhibited stem elonga
    tion of petunia compared to plants grown in continuous LD, while it did not affect crown diameter and branch
    number at first flower. Suggestionswere p resented that petunia Fantasy should be p lanted under long days up
    on 8-leaf stage for fast flowering, and planted under short days after 3 weeks for saving energy, and the plant
    height can be shortened at the same time.
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  • Effects of Nitric Ox ide on Respiration and Enzymatic Activity of Cut RoseDuring Va se
  • ZHANG Shao-ying;RAO Jing-ping;REN Xiao-lin
  • Acta Horticulturae Sinica. 2007, 34(1): 183-188.
  • Abstract ( 1859 ) HTML ( 2016 ) PDF (528KB) ( 2016 )    
  • Using sodium nitrop russide ( SNP) as a nitric oxide (NO) donor and its scavenger PTIO (2-phenyl-4, 4, 5, 5-tetra
    methylimidazoline-1-oxyl-3-oxide) , the effects of nitric oxide treatment on respira
    tion, ethylene p roduction, relative membrane permeability, MDA content and activities of COX ( cytochrome
    oxidase) , AO ( ascorbic acid oxidase) and PPO (polyphenol oxidase) of cut rose were studied. The results
    showed that NO resulting from 0.1 mmol·L-1 SNP aqueous solution decreased wilt rate, increased fresh
    mass, p rolonged vase life, inhibited COX, AO and PPO activities, decreased the resp iration rate and ethylene
    p roduction, delayed the resp iration and ethylene peaks, and slowed down the increase ofMDA content and the
    relative membrane permeability. The treatment of PTIO stimulated COX, AO and PPO activities, increased
    the resp iration rate, accelerated the increase ofMDA content and relative membrane permeability under SNP
    treatment, but inhibited the ethylene p roduction. Those results indicated that NO might participate in regula
    tion of terminal oxidases in resp iration.
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  • Effect of Exogenous Salicylic Acid on Vase Life of Cut Flowers of ‘Prato’Lily and Related Physiological Influence
  • PENG Xiao-li;RAO J ing-ping;ZHANG Yan-long
  • Acta Horticulturae Sinica. 2007, 34(1): 189-192.
  • Abstract ( 1978 ) HTML ( 1255 ) PDF (486KB) ( 1255 )    
  • In this experiment, freshly harvested Asiatic hybrid lilies were treated with fundamental pre
    servative ingredients ( 20 g·L-1 Sucrose, 250 mg·L-1 8-HQ, 1 g·L-1 CaCl2 ) plus 35 mg·L-1 salicylic
    acid, and then the p reservation effectswere investigated. The results indicated that the p reservative containing
    salicylic acid p rolonged vase life and the longevity of individual flowerlets, increased the fresh mass and solu
    ble protein content, lowered the respiration rate and ethylene evolution, delayed the occurrence of resp iration
    and ethylene peaks, retarded the chlorophyll degradation in the leaf. It also decreased malondialdehyde
    (MDA) and free proline accumulation in the petal. Both the basic p reservative and the basic preservative plus
    salicylic acid were effective in preventing leaf yellowing.
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  • Studies on Crucial Technology of Rapid Asexual Propagation and CommercialProduction of Phalaenopsis
  • GU De-feng;ZHAO Chun-li;SONG Yan-jun;GU Su-min;ZHANG Jia-xu;ZHANG Hong-wei
  • Acta Horticulturae Sinica. 2007, 34(1): 193-196.
  • Abstract ( 1575 ) HTML ( 1214 ) PDF (247KB) ( 1214 )    
  • The major technology which affecting rapid asexual propagation and commercial production of
    Phalaenopsiswas discussed. Results showed that the type of culture medium and the time of subculture directly
    affected the p ropagation rate of its p rotocorm. The propagation rates of protocorm in the modified Kyoto and KC
    media were significantly higher than that in MS medium. The op timal subculture time was 2.5 months. The
    maturity of seedlingswas accelerated by using two step s cultivation, and it only needed seven or eightmonths
    in tube. According to investigation on the abloom p lant of p rotocorm of five varieties p ropagated for 20 genera
    tions, the results showed that the colony characters of these blooming plants are stable and the rate of variation
    of two protocormswas lower than 1 /10 000 in the propagation of 18th - 20th generations.
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研究简报

  • In Vitro Ovary Culture of Cardiocrinum giganteum
  • LI Shou-li;;SHI Lei;ZHANG J in-zheng;ZHUANG Ping
  • Acta Horticulturae Sinica. 2007, 34(1): 197-200.
  • Abstract ( 1910 ) HTML ( 1437 ) PDF (347KB) ( 1437 )    
  • An experiment was carried out on the induction of bulbulets and the plant regeneration by
    using the ovaries of Cardiocrinum giganteum as exp lants. The results indicated that BA and KT were the main
    factors in ovary explant differentiation. The explants differentiated into calluses when the concentration of BA
    and KT was between 0.1 and 1.0 mg/L and budswere induced when the concentration was bewteen 2.0 and
    4.0 mg/L, and when higher than 4.0 mg/L, leaves formed. The best basic media for the dedifferentiation
    were N6 and B5. The bestmedium for bulbulet induction from calluswasMS + 0.1 - 0.5 mg/L NAA + 2.5
    mg/L BA + KT 2.5 mg/L + 10% sucrose + 0.7% agar. The ratio of root induction from calluswas 100% on
    the medium of 1/2MS + 3% sucrose + 0.7% agar + 1% active carbon. After acclimatization for one week,
    the bulblets were transp lanted into greenhouse and all grew well.
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  • Effect of IBA and Agrobacterium rhizogenes on the Softwood Cutting of Tiliamandshurica
  • ZHANG Qin;L I Bao-hui;DUO Jian-guo;WANG Wen-feng;LIU Yun-qiang;LIANG Hai-yong;YANG Jian-min
  • Acta Horticulturae Sinica. 2007, 34(1): 201-204.
  • Abstract ( 1884 ) HTML ( 1736 ) PDF (269KB) ( 1736 )    
  • The influence of IBA and Agrobacterium rhizogenes on the softwood cutting of Tilia m andshuri
    ca was studied during rooting, while the mechanism of their effects on the rooting was discussed. The results
    showed that the rooting percentage and number of roots significantly increased ( P < 0.01) and the rooting
    time decreased after treatment with IBA and Agrobacterium rhizogenes. IBA and Agrobacterium rhizogenes also
    increased the IAA /ABA ratio and contents of IAA, GA3 , soluble sugars, reduced ABA contents of cuttings
    during the rooting period, and p romoted remarkably rooting of softwood cutting. It was showed that the treat
    mentwith IBA + Agrobacterium rhizogenes was the best. The rooting percentage, quantity of root and root
    length of the cutting after treatmentwith IBA +Agrobacterium rhizogenes increased by 122.08%, 65.75% and
    23.63% , respectively, while the rooting period shortened by 14 days compared with control.
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  • Cloning and Expression Analysis of a Sucrose Phospha te Synthase cDNAFragment in Melon Fruit
  • YU Xi-yan;FAN Ji-de;HE Qi-wei
  • Acta Horticulturae Sinica. 2007, 34(1): 205-208.
  • Abstract ( 1463 ) HTML ( 1347 ) PDF (638KB) ( 1347 )    
  • PCR p rimerswere designed based on the conserved domain of some sucrose phosphate syn
    thase genes in GenBank. The target cDNA fragmentwas amp lified from the total RNA isolated from melon fruit
    of 25 days after pollination with RT-PCR and then was cloned into pMD18-T vector. The sequence showed that
    the amino acid sequence encoded by this fragment showed a 98.9% similarity to that of the corresponding re
    gion of sucrose phosphate synthase gene in tomato. The accession number of this gene in GenBank was
    DQ355797. Northern blotwas performed to analyze the exp ression pattern of sucrose phosphate synthase in dif2
    ferent development stages of melon fruit. The result showed that sucrose phosphate synthase began to exp ress
    in fruit 25 days after pollination and the expression level of this gene increased with melon fruitmature.
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  • Expeimental Study on POD Activity Passivation of Strawberry by HighHydrostatic Pressure
  • HUANG Xun-duan;;XIE Hui-ming;PAN Jian;ZENG Qing-mei;YANG Yi;WANG Hai-xiang
  • Acta Horticulturae Sinica. 2007, 34(1): 209-212.
  • Abstract ( 1438 ) HTML ( 1642 ) PDF (454KB) ( 1642 )    
  • The passivation effect of high hydrostatic p ressure on POD ( peroxidase) of strawberry was
    studied by response surface methodology. Experimental results showed that high hydrostatic p ressure could
    passivate POD significantly. Based on analysis of the factors (p ressure, temperature and time) and their inter
    action, it was found that p ressure was a key factor to passivate POD under the conditions of this experiment.
    A model, y = 97.2 - 44.0x1 - 1.1x2 - 5.3x3 - 27.5x12 - 7.3x22 - 9.4x32 - 8.3x1x2
    - 5.0x1 x3 + 26.9x2 x3 ,
    was established by Design Expert software. The determination coefficient of model was 0.9883 and adjusted
    determination coefficientwas 0.9732. Analysis of variance imp lied that the modelwas significant. Therefore,
    the model could be used to analyse and predict activity passivation of POD.
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  • AFLP Markers of Cucumber Anthracnose Resistance-related Gene
  • WANG Hui-zhe;LI Shu-ju;LIU Xiu-feng;LI Ping;HUO Zhen-rong;GUAN Wei
  • Acta Horticulturae Sinica. 2007, 34(1): 213-216.
  • Abstract ( 1366 ) HTML ( 1320 ) PDF (325KB) ( 1320 )    
  • With a F2 population derived from 66 ×A18 asmaterials, polymorphism between resistant and
    suscep tible bulk of cucumber anthracnose were studied using BSA method and AFLP technology. A codomi
    nantAFLP marker, E24M48=251 bp /245 bp, was screened. The resistant and suscep tible F2 plants possessed
    the 251 bp and the 245 bp fragment, respectively, and the mid-plants owned the two fragments. The AFLP
    markerwas closely linked with the cucumber anthracnose resistance related gene, and the genetic distance be
    tween the marker and gene was 21727 cM. Fragements sequencing of the codominantAFLP marker indicated
    that their length were 251 bp and 245 bp, respectively. The difference of the two fragements lied in the insert
    deletion of two “TCT” repeat sequences.
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  • Identification of SCAR Markers Linked to Orange Head Leaf Gene inChinese Cabbage ( Brassica rapa L. ssp. pekinensis)
  • WANG Guo-chen;ZHANG Feng-lan;YU Yang-jun;ZHANG De-shuang;ZHAO Xiu-yun
  • Acta Horticulturae Sinica. 2007, 34(1): 217-220.
  • Abstract ( 1507 ) HTML ( 1297 ) PDF (393KB) ( 1297 )    
  • Bulked segregant analysis was emp loyed to identify molecular markers linked to orange head
    leaf gene ( or) trait based on RAPD and AFLP in Chinese cabbage with a doubled hap loid (DH) population
    derived from the F1 between the two parents 91-112 (white head leaf) and T12-19 ( orange head leaf) via
    microspore culture. One RAPD marker S1338656 and one AFLP marker P67M54172 were identified to be linked
    to or gene with the distance of 8 cM and 13 cM respectively. These two markerswere successfully transformed
    into SCAR markers SCOR204 and SCOR127. The accuracy of 90% and 89.1% were obtained when 110 differ
    ent incompatibility breeding lines of Chinese cabbage were used to be investigated with two SCAR markers, in
    dicating that these two SCAR makers could be used in marker2assisted2selection in orange head leaf breeding
    p rograms and positional cloning of or gene in Chinese cabbage.
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  • Physiological and Biochemical Changes of Onion with Different MaturitiesDuring the Dormancy Stage
  • CHEN Qin-bin;HOU Xi-lin;WANG Jian-jun;HAN Jian-ming
  • Acta Horticulturae Sinica. 2007, 34(1): 221-224.
  • Abstract ( 2026 ) HTML ( 1571 ) PDF (246KB) ( 1571 )    
  • The dynamic changes of RR ( resp iratory rate) , SPC ( soluble p rotein content) , SSC ( sol
    uble sugar content) , SOD, POD, endohormone IAA and ABA in different parts of three onion varieties ( in
    cluding early2maturing, middle2maturing and late maturing types) with differentmaturities during the dorman
    cy stage were studied. The results suggested that the length of physiological dormancywas different among the
    three varieties, the dormancy period of the late2maturing variety Gangcong 841 was the longest ( about 70 d)
    and the early2maturing variety Yangchunhuang was the shortest ( about 40 d). The resp iratory rate, SPC and
    the activity of SOD, POD showed a rap id rising trend and then falling trend before the end of physiological dor
    mancy. The SSC decreased rap idly after physiological dormancy. The content ofABA was highest in the early
    dormancy period, afterwards, a rap id falling until the end of physiological dormancy. Change of IAA was
    rightly reverse to ABA. The distribution of these physiological and biochemical data were interior scales >mid
    dle scales > exterior scales. It is concluded that the higherABA, lower IAA, SPC and higher SSC are benefi
    cial to remain dormancy after harvest.
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  • Anthocyan in Accumulation and CHS, DFR Gene Expression Regula ted byLight and Sugar in Gerbera hybrida Ray Floret
  • MENG Xiang-chun;PENG Jian-zong;WANG Xiao-jing
  • Acta Horticulturae Sinica. 2007, 34(1): 227-230.
  • Abstract ( 1741 ) HTML ( 2033 ) PDF (543KB) ( 2033 )    
  • Effects of light and sugar on Gerbera hybrida ray floret ( rf) p igmentation were investigated.
    Inflorescences or rfs at stage P2 were detached and incubated on the medium (8 g·L-1 agarwith 3% sucrose
    or free of sucrose) under 14 h light /10 h dark cycles at 23 - 25℃. Cultured inflorescences and rfswere first
    incubated in darkness for 3 d, then exposed to transient light for a period of time followed by an incubation in
    the dark until harvesting for anthocyanin determination by spectrophotometer at 9 th d or harvested immediately
    for gene exp ression detected byNorthern-blot. The results showed that transient light exposure induced antho
    cyanin accumulation and the gene exp ression of CHS and DFR in rf petal after 3 d p reculture in darkness. The
    longer time of light irradiation strongly enhanced the gene exp ression of CHS and DFR . Petals precultured in
    darkness for 7 d and 9 d had weak response to light in gene expression. When sucrose was present in the me
    dium, both anthocyanin accumulation and gene exp ression were strongly increased under light condition.
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  • Analysis on the Aroma Components in Several Species of Hedychium
  • FAN Yan-ping;WANG Xu-ri;YU Rang-cai;YANG Ping
  • Acta Horticulturae Sinica. 2007, 34(1): 231-234.
  • Abstract ( 1586 ) HTML ( 1478 ) PDF (434KB) ( 1478 )    
  • Hedychium is a perennial herbaceous genus belonging to Zingiberaceae. Aroma production
    differs among different Hedychium species. Studies on the aroma componentswill help uswith the understand
    ing of their biosynthesis and with the breeding of new varieties via bioengineering. In this study, gas chroma
    tography-mass spectrometry combined with solid-phase microextraction ( SPME - GC - MS) was developed for
    the determination of aroma components in four different Hedychium s species, namly H. coccineum, H. gard
    nerianum
    , H. f lavum and H. coronarium , which have different fragrance. The results showed that the aroma
    components and contents in the four Hedych ium s specieswere different. The relative aroma content in H. coc
    cineum was only 1.21 μg·h-1·g-1 FM, but that in H. gardnerianum , H. f lavum and H. coronarium were
    16.80, 13.64 and 17.36 μg·h-1·g-1 FM, respectively. L-linalool and 1, 8-cineole might be the charac
    teristic aroma components in Hedychium.
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  • Oil Extract from Passionflower Seed by Supercritical CO2 and It,s Composition Analysis
  • OUYANG Jian-wen;XIONG Xing-yao;WANG Hui-xian;WANG Ren-cai;LIU Dong-bo;YANG Guo-ping;YUAN Hong
  • Acta Horticulturae Sinica. 2007, 34(1): 239-241.
  • Abstract ( 1739 ) HTML ( 1255 ) PDF (211KB) ( 1255 )    
  • The technical system of oil extracting from passionflower ( Passiflora edulis Sims. ) seed using
    supercritical CO2 extracting equipmentwas studied. The op timal seed oil extractive processwas as follows: the
    extraction pressure was 30 MPa, the extraction temperature was 45℃, the separation temperature was 40℃
    and the extraction time was 70 min. Under these conditions, the extraction yield was 28.4%. Twelve compo
    sitions of the oil was identified by gas chromatography2mass spectrometry (GC - MS). The main composition
    was fatty acid and unsaturated hydrocarbon. There was 7518% of unsaturated fatty acids in total fatty acid and
    1714% of squalene in unsaturated hydrocarbon
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  • RAPD Analysis on the Genetic Relationsh ips of Tea Cultivars Grown inSichuan
  • WANG Xue-ping;MA Bing-tian;QI Gui-nian;TIAN Hong;FANG Chang-you;ZHANG Ze-cen;YIN Xu-min
  • Acta Horticulturae Sinica. 2007, 34(1): 242-244.
  • Abstract ( 1557 ) HTML ( 1382 ) PDF (432KB) ( 1382 )    
  • The genetic relationship of 36 tea cultivars grown in Sichuan was studied by RAPD technique.
    Sixteen primers selected from 50 random p rimerswere used to amp lify 36 tea samples, and a total of 167 DNA
    bandswere amplified, among which 158 (94.61% ) were polymorphic. Genetic distances between the culti
    vars varied from 0.149 to 0.679. UPGMA cluster analysis of 167 bands amplified by 16 p rimers was made.
    The result showed that 36 tea cultivars tested could be classified into 3 comp lex group s and 2 simp le group s. It
    was found that there was a certain correlation between the results and the derivation of tea cultivars.
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综述

  • Advances in Studies on Genus Lagerstroemia
  • ZHANG Jie;WANG Liang-sheng;ZHANG Jing-jing;SHU Qing-yan;GAO Jin-ming
  • Acta Horticulturae Sinica. 2007, 34(1): 251-256.
  • Abstract ( 1672 ) HTML ( 1862 ) PDF (281KB) ( 1862 )    
  • The advances in researches on germp lasm resources conservation, cultivation, p ropagation and
    sustainable utilization of crape myrtle ( genus Lagerstroemia) were reviewed. In the meantime, the progress of
    ornamental traits such as p lant shape, flower color, scent and form were studied. In the future, it should be
    emphasized to carry out research on conserving core collection, enriching flower color and scent, selecting and
    breeding novel cultivars, and deepening studies on their pharmaceutical components.
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新品种

  • Juic ing Apple Variety ‘Judeline’
  • SONG Ye;ZHAI Heng;YAO Yu-xin;LI Fu-jun;LIU Jin-bao
  • Acta Horticulturae Sinica. 2007, 34(1): 257-257.
  • Abstract ( 1556 ) HTML ( 1128 ) PDF (142KB) ( 1128 )    
  • Judeline (M. domestic ‘Judeline’) is a juicy apple variety selected from processing app levarieties introduced from France bymultiple regional spot trials. It has good characteristics such as early fruit
    ing, high yielding, excellent juicy quality and nice taste. W ith good ecological adap tability, it is a good p ro
    cessing apple variety for unp roductive land.
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  • Extra-late-ripening Juice Making Grape Variety ‘Beixiang’
  • FAN Pei-ge;LI Sheng-chen;YANGMei-rong;LI Shao-hua
  • Acta Horticulturae Sinica. 2007, 34(1): 259-259.
  • Abstract ( 1655 ) HTML ( 1242 ) PDF (94KB) ( 1242 )    
  • Beixiang is an interspecific hybrid cultivar derived from Vitis thunbergii andMuscat of Alexan
    dria (V. venifera). Berriesmature during the first 10 days of October in Beijng. The vines are vigorous with
    high yeild. The fruit cluster is conical, weighing 194.8 g in average. The average mass of a single berry is 2.21
    g. The moderately crowded berries are uniformly sized, ellip tical and purple black. The flesh is soft and juicy.
    The juice is purp le red and acid-sweet with 18.6% soluble solid content, 0.63% titratable acid content and
    80.6% juice extraction. It has high resistance to cold and diseases.
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  • A New Soft-seeded Pomegranate Variety ‘Hongmanaozi’
  • ZHAO Guo-rong;ZHU Li-wu;ZHANG Shui-ming;JIA Bing;LI Shao-wen
  • Acta Horticulturae Sinica. 2007, 34(1): 260-260.
  • Abstract ( 1773 ) HTML ( 1093 ) PDF (93KB) ( 1093 )    
  • Hongmanaozi is a new variety with soft seed selected from the mutant of Punica granatum
    ‘Manaozi’ by RAPD-PCR-assisted technique. The average fruitmass, 100-grain mass, soluble solid content
    and juice content of Hongmanaozi increased by 16.8%, 16.4%, 3.0% and 1.1% , respectively, in compari
    son with that ofManaozi. The seed average hardness of Hongmanaozi is 3.56 kg/ cm2. It is p roductive and has
    high resistance to diseases caused by Iythia versoniana Sau. and Cercospra punicae P. Henm. The fruitsma
    ture in early October in the mid-area of Anhui Province.
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  • A New Bayberry Variety‘ Zaotanmei 8801’
  • WANG Bai-po;CHENG Xiao-jian;YU Wei-wu;FU Qing-gong
  • Acta Horticulturae Sinica. 2007, 34(1): 262-262.
  • Abstract ( 1772 ) HTML ( 1548 ) PDF (143KB) ( 1548 )    
  • Zaotanmei 8801, a new cultivar, was selected from the bayberry (Myrica rubra Bieb) cultivar
    Biqi, Its fruits begin to mature on June 10 th, 10 - 15 days ahead of Biqi cultivar, and 17.86% bigger than
    the early ripening strains. Its p roduction is 3.5 kg/m2 within the crown diameter. As a multifunctions cultivar,
    Zaotanmei 8801 can be p lanted formidable eco-environment, such as red earth and low-hill strip s. The fruits
    of Zaotanmei 8801 can be used for fresh consump tion and processing, and have a high and stable yields.
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  • A New Tomato Hybrid ‘Shalong’
  • HUANG Ting-ting;LIU Bing-lu;LIU Shu-qin;WANG Chang-yi;LI Zhe-qing
  • Acta Horticulturae Sinica. 2007, 34(1): 264-264.
  • Abstract ( 1488 ) HTML ( 919 ) PDF (90KB) ( 919 )    
  • Shalong is a new mid-ripening tomato hybrid. It is resistant toMV, fusarium wilt and southern
    root-knot nematode disease, mid-type fruit, regular shape, good commodity and high yield. The yield ismore
    than 150 000 kg/hm2. It has good storage tolerance and its shelf life is 18 days at room temperature (25℃).
    The variety is suitable for protected field and open field production.
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  • A New Fresh-eating Small Tomato Variety ‘Jidong 216’
  • MAO Xiu-jie;WANG Jiu-xing;WANG Ju-yuan;CAO Xia;WEI Yu-tang
  • Acta Horticulturae Sinica. 2007, 34(1): 265-265.
  • Abstract ( 1488 ) HTML ( 1123 ) PDF (94KB) ( 1123 )    
  • J idong 216 is a F1 by crossing the domestic varietywith import small tomato. It has unlimited
    branches and common leaf and grows vigorously. The first inflorescence is on the middle of the sixth and
    eighth node and each inflorescence bears 8 - 10 tomatos. The fruit is red and very tasty. Fruit shape index is
    about 0.9. The average fruitmass is 38 g. The content of vitamin C is 175.0 mg/kg, organic acid is 1.0%
    and the ratio of sugar to acid is 5.5. It is high-resistant to leafmold and southern bacterialwilt, mid-resistant
    to TMV and gray mold. It is suitable for p rotected cultivation
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  • Sweet Potato Variety ‘Baishu 1’
  • OU Xing-qi;REN Xiu-juan;LI Xin-hua
  • Acta Horticulturae Sinica. 2007, 34(1): 266-266.
  • Abstract ( 2191 ) HTML ( 993 ) PDF (445KB) ( 993 )    
  • Baishu1 is a new leaf-vegetable sweet potato variety which is a self-bred progeny variety from
    Anshu 07. Leaf-vegetable Baishu 1 has many merits: half-erect plant types 25 - 30 branches at stem base,
    delicate stem the longest stem about 80 - 100 cm, small leaf, no pubescence on the vine and tastes good. The
    yield of sweet potato tip s ismore than 22.5 t/hm2. It is suitable to be planted in field and protective area.
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