Effects of exogenous NO3-on sucrose, glucose, fructose and starch contents as well as sucrose synthase(SS) disassemble activity, sucrose synthase synthesis activity, neutral invertase activity(NI)and acid invertase activity(AI)in roots of sweet cherry ( Prunus. avium. 'Daihong') with P.pseudocerasus var Daqingye as rootstock under water-logging condition were studied. The results showed that under water-logging condition, sucrose, glucose, fructose and starch in roots of sweet cherry increased significantly in the early period and then decreased in the later period in both treatment and control. Exogenous NO3-enhanced the contents of sucrose, glucose, fructose and starch, which were higher than control, while to the maximum, which were 18.02%, 21.97%, 25.27% and 11.41% higher than that of control respectively when reached to the maximum. The sucrose synthase disassemble activity in roots of sweet cherry increased to the highest level and then declined gradually in both treatment and control. Meanwhile, the sucrose synthase synthesis, NI and AI activities decreased gradually, but four enzymes activities of treatment were higher than that of control after using exogenous NO3-. We conclude that the addition of NO3- can enhance the tolerance of the roots of sweet cherry to hypoxia through regulation of sugar metabolism.
The effects of NAA, IBA, ZT and 6-BA on the growth and the changes on endogenous levels of PAs and nitric oxide were investigated by using regeneration system of Malus hupehensis Rehd. in vitro. As a result, the increase rate of fresh weight and the contents of protein, polyamines (PAs) and nitric oxide (NO) increased significantly in MS medium supplemented with 0.02-1.00 mg·L-1 NAA or IBA, and the increases were the highest at concentrations of 0.10 - 0.50 mg·L-1. One more thing,the effects of NAA on the increase rate of fresh weight and putrescine content were higher than that of IBA at the same concentration. With the increase concentration of ZT or 6-BA at 0.08 - 4.0 mg·L-1, the increase rate of fresh weight, protein, PAs and NO contents increased gradually. Moreover, the effects of 6-BA on increase rate of fresh weight and putrescine content is higher than that of ZT at the same concentration. Auxin and cytokinin promoted the growth accompanied with the elevated production of PAs and NO, and the growth dynamics showed the similar trend with the contents of PAs and NO in explants in vitro.
A cDNA library was successfully constructed via suppression subtractive hybridization between fruit epicarp (experimental group, tester) and pulp (control group, the driver) of Ponkan(Citrus reticulata Blanco). Results showed that most of the clones had inserting fragments which length ranged from 100 bp to 500 bp. Four hundred and eleven ESTs were successfully sequenced and searched against NCBI GenBank, The results indicated that 377 ESTs had significant similarities with known genes. All the ESTs were divided into 126 genes according to the gene annotation. These ESTs were mainly involved in pigment synthesis, energy metabolism, primary metabolism, secondary metabolism, defense against stresses, signal transduction, transcription, flavor formation, oil synthesis, peel softening, antioxidant metabolism et c. There were 63 uni-ESTs which accounted for 49.2% of the total ESTs and another 34 ESTs had no matching homologous sequences found.
Parthenocarpy of 75 cucumber germplasm and cultivars was evaluated under greenhouse condition in spring season. The results showed that the parthenocarpy percentage of 75 accessions was continuously distributed from 0 to 100% . While 40 accessions were found strongly parthenocarpy (53.3%), 24 were medium (32%) and 11 were weakly parthenocarpic (14.7%). 20 out of the 24 cultivars were found as strong parthenocarpy (83.3%), and the rest was mid-parthenocarpy. The degree of parthenocarpy was expressed by the fruit set capability, and the fruit weight and fruit shape index of the seedless fruit present a good similarity with that of the seeded. The cucumber plants get stronger parthenocarpic with the nodes increase and the best evaluation should be done under 20th nodes.
Four generations of P1, P2, F1 and F2 were used to study the inheritance of melon sugar content、sour content and sugar acid radio traits in mutation inbred line with sour taste (76-2 by 60Co- γ irradiation) and 'Huangpicui' by joint analysis method of multiple generations. The results showed that the sugar content was controlled by two pairs of equal additive major genes plus additive-dominant polygene (E-4 model). The major gene heritability is 88.8%; the polygene heritability is 6.94%. The inheritance of sour content fitted one pair of additive-dominance major gene plus additive-dominance-epitasis polygene (D-0 model). The major gene heritability is 26.68%; the polygene heritability is 72.77%. Sugar acid radio trait fitted two pairs of additive-dominance-epitasis major genes plus additive-dominant polygene (E-1 model). The major gene heritability is 82.86%; the polygene heritability is 16.02%.
R3a and RB confer resistance to potato late blight by recognizing the effector protein Avr3a and Avrblb1 of Phytophthora infestans. R gene mediated resistance requires multiple signal transduction pathways. Virus-induced gene silencing (VIGS) is a powerful tool for studying genes participation in signal pathways. Eight genes, SIPK, NDR1, SGT1, HSP90, NPR1, Rar1, EDS1 and WRKY1 from other species are known to be involved in defence-related signal pathway. Expression of each gene was suppressed by VIGS in Nicotiana benthamiana and the effect was determined on Hypersensitive Response symptoms induced by RB-Avrblb1 and R3a-Avr3a. R3a- and RB-mediated resistance was compromised by the silencing of HSP90 and SGT1, suggesting that the RB-Avrblb1 and R3a-Avr3a interaction are dependent on ubiquitin ligase-associated protein SGT1 and heat-shock protein HSP90. The establishment of this system will identify new genes involved in resistance to late blight.
Effects of soil water on plant growth, contents and components of essential oil in fennel were studied , by controlling soil water content at low (relative water content, 44.46%±1.22%~52.84%±2.34%), middle (relative water content, 59.75%±3.11%~77.85%±3.47%) and high (relative water content, 75.24%±3.24%~99.27%±5.67%) levels. The results showed that, with increasing of soil water content , plant height, number of node, root and shoot fresh weight(FW), plant FW, content of total carbon, yield of essential oil per. plant and Chl.a/Chl.b ratio were elevated, but root/shoot ratio, contents of total nitrogen, proteinic nitrogen, Chl.a, Chl.b and CAR were decreased; Number of inflorescence, contents of essential oil and solubility sugar were higher in middle level water treatment, while they were lower in low and high level water treatments; Twenty-two chemical compounds were also identified in different soil water treatments, relative content was no difference (α=0.05) in α-Pinene, Sabinene, β-Pinene, γ-Terpinene, Terpinolen and Myristicin, but was significant ( α=0.05,0.01) in other sixteen compounds; With increasing water content , content of (E)-anethole, the dominant component in essential oil, was enhanced at 41.34%, 53.87% and 62.97%; Limonene was the second abundant material in oil, its content was decreased at 44.77%, 34.77% and 22.16%. This study indicated that lower water content had a positive role in accumulation of Limonene, and higher water content was relatively favour for storage of (E)-anethole in fennel.
To study different structural genes expression pattern in anthocyanin biosynthesis pathway of cineraria with different flower color, internal segments of five structural genes, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H) and dihydroflavonol 4-reductase (DFR), were isolated from cineraria petals using RT-PCR. Using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), the expression patterns of CHS, CHI, F3H, F3'H, DFR, flavonoid 3',5'-hydroxylase (F3'5'H) and anthocyanin 3-O-glucoside-6"-O-malonyltransferase (3MaT) in five developmental stages of cineraria with different flower colors were determined for the first time. The results indicated that there is no CHS transcript in white flowers. In red flowers, F3'H mRNA was expressed at high level. But no F3'5'H transcript was found. F3'5'H was highly expressed in Stage I but no F3'H mRNA was observed in blue flowers. And the transcripts of F3'H and F3'5'H were found in purple petals. In addition, these genes were expressed at high level in early stage and underwent moderate decreases in expression. Interestingly, genes expression level increased again in Stage IV. However, in Stage V, there is little expression level could be detected in petals.
The initiation and development of the bulbet of Lilium oriental ‘Sorbonne’ were studied by slicing up and histochemistry coloration. It was showed that the bulblet originated from 8-14th layer inner parenchyma cells near the axle of the scale. the development of bulblet could be diveded four stages according to morphogenesis changes. The saccharide was existed in meristematic tissue and vascular organization with mainly grain-like form, which was indicated that high content of saccharide could induce vascular organization, otherwise the protein was mainly distributed around nuclei duriong bulblet induction. The concentration gradient of saccharide and protein. was observed between meristematic tissue and surrounding parenchyma cells with higher saccharide and lower protein in the differentiation tissueand.
To determine the chilling tolerance of cucumber fruit after harvest, cucumber (Cucumis sativus L. ‘Jinyou 1’) fruit were stored at 2 °C for 9 days before ripening at 20 ℃ for 2 days. Chilling injury index, electrolyte leakage, activities of phospholipase D (PLD) and lipoxygenase (LOX), membrane-associated Ca2+ content in the calyx, middle and stalk sections of cucumber fruit were determined, respectively. Chilling injury occurred initially in the calyx area, and developed toward the middle and stalk end. After storage, chilling injury index, electrolyte leakage, activities of PLD and LOX in calyx section were highest, and lowest in stalk section of the fruit. Content of membrane-associated Ca2+ increased from calyx to stalk. Date indicated that increasing tolerance to chilling temperature from calyx to stalk of cucumber fruit was significantly related with the stimulation of PLD and LOX activities and decrease of membrane-associated Ca2+ content.
On the basis of anatomical observation, the postharvest stem blockage in cut gerbera (Gerbera jamesonii) flowers and the effect of the nano silver treatment were investigated.The results showed that the obvious vascular blockage was found in the cut stem end,but not in the stem segments of upper and middle parts of cut gerbera flowers. Observation under SEM certified that the vascular blockage was resulted from bacteria. Pulsing the stem end of cut gerbera flowers for 24 h in nano silver solution, the vascular blockage could be significantly alleviated and delayed, and the vase life was also markedly prolonged.