果树

• Palynological Study on the Origin and Systematic Evolution of Kernel-using Apricots
• LIU You-chun;CHEN Wei-zhi;LIU Wei-sheng;LIU Ning;ZHANG Yu-ping;and LIU Shuo
• Acta Horticulturae Sinica. 2010, 37(9): 1377-1387.
• Abstract ( 2081 ) HTML ( 1833 ) PDF (361KB) ( 1833 ) 　　　
• The characters of pollen grains from 20 apricots representing Prunus armeniaca L.，P. sibirica L. and kernel-using apricots of subgenus Armeniaca were investigated with scanning electron microscopy（SEM）cluster analysis，using average linkage means，was then conducted with 10 quantitative and 2 qualitative traits from palynological data. The results showed that all pollen grains of subgenus Armeniaca were monads，isopolar，radially symmetrical，triangular or obtuse-triangular in the polar views，elliptical in the equatorial views，and the pollen was tri-colporate，which was consisted of an ecto-porus and a colpus，the ecto-porus obviously convex，elliptic，covered with irregular reticulate-like pattern and independent of the exine，the colpus usually elongated，entending nearly to the two poles. Pollen with striate ornamentation，presence or absence for perforation，four appurtenance types on the exine surface，such as turnup-like，ridge-like，granular and verrucate，and it is suggested that the between different apricot accessions could be used as important indicator for classification and phylogenetic relationships analysis in this subgenus. Both cluster and principal coordinate analysis classed 20 apricots into three groups：Kernel-using apricots group，mixed group of P. sibirica and P. armeniaca and mixed group of P. armeniaca and kernel-using apricots. The present study suggested that the most kernel-using apricots originated from the natural hybrid of P. armeniaca and P. sibirica，some may also be directly originated from sweet kernel type of P. armeniaca. Moreover，P. sibirica should be more evolutionary than P. armeniaca.
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• Characteristics Analysis of Microelement Contents in Newhall Navel Orange Leaves in Southern Jiangxi Province
• LING Li-li;PENG Liang-zhi;;CHUN Chang-pin;CAO li;and JIANG Cai-lun;
• Acta Horticulturae Sinica. 2010, 37(9): 1388-1394.
• Abstract ( 1932 ) HTML ( 1568 ) PDF (62KB) ( 1568 ) 　　　
• To probe the causes of leave yellowing of Newhall navel orange in southern Jiangxi Province，leaf microelements of Newhall navel orange（Citrus sinensis Osbeck）trees on trifoliate orange [Poncirus trifoliata（L.）] rootstocks in 121 orchards in southern Jiangxi Province were analyzed. The results showed that the contents of Fe，Mn，Zn，Cu，B and Mo were（127.90 ± 21.15），（115.79 ± 136.72），（16.50 ± 4.05），（8.42 ± 9.32），（81.88 ± 33.72）and（0.36 ± 0.14）mg · kg-1，respectively. The orchards having leaf Mo，Fe，Mn，B，Cu and Zn with moderate content and above accounted for 100%，99.17%，90.91%，92.56%，62.81% and 4.96%，respectively，and those with low content for 0.83%，7.44%，7.44%，29.75% and 23.97%，respectively. Deficiency of leaf Mn，Cu and Zn were found in some orchards，1.65%，7.44% and 71.07%，respectively. The results indicated that generally there were no leaf Mo，Fe，Mn and B deficiencies in Newhall navel orange in this area. But leaf Cu content in some orchards was low，andmost orchards had leaf Zn deficiency problems. It is recommended that attention should be paid to Zn fertilizer application in navel orange orchards and Cu fertilizer is needed for the orchards with low leaf Cu content.
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• Variation of Soil Microbial Populations and Relationships Between Microbial Factors and Soil Nutrients in Cover Cropping System of Vineyard
• XI Zhu-mei;LI Hua;LONG Yan;ZHANG Jin;and PANG Xue-liang
• Acta Horticulturae Sinica. 2010, 37(9): 1395-1402.
• Abstract ( 1941 ) HTML ( 1484 ) PDF (108KB) ( 1484 ) 　　　
• Three such cover crops，two perennial legumes (white clover and alfalfa) and a perennial gramineous grass（tall fescue）were sown inter-row in Cabernet sauvignon vineyard，with clean tillage as the control. The effects of cover cropping on microbial populations，soil nutrient contents and their relationships were studied. The results showed that soil microbial quantities in cover cropping treatments were increased，among which azotobacter and cellulose-decomposing and bacteria microorganisms increased by 223.4%，83.4% and 68.1%，respectively，but only slightly changing for actinomyces，compared with bare soil（control），the soil from white clover and alfalfa plots showed higher quantities of microbial populations than tall fescue polt，and significant differences were observed between the two cover crop treatments and the control（except actinomyces）. Also，it indicated that cover crop treatments had higher soil organic matter content，but reduced available P and total P. For white clover and alfalfa treatments，the contents of soil hydrolyzable N，total N，available K increased evidently，while decreased for tall fescue treatment. All kinds of soil nutrients showed significant or very significant position correlations with soil microorganism factors，except that soil available P were no significant correlations with fungi and azotobacter. Path analysis indicated that in the vineyard intercropping system，soil cellulose-decomposing microorganisms was the main factor affecting the accumulation of soil organic matter，soil actinomyces was the most important factor affecting soil total N，hydrolyzable N，total P，available P and available K.
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• Changes of Hormones，Polyamines and NO Content During Regeneration of Adventitious Buds from in Vitro Leaves of Red Fuji Apple
• TIAN Chun-ying;SHAO Jian-zhu;LIU Ying;and XU Ji-zhong;
• Acta Horticulturae Sinica. 2010, 37(9): 1403-1408.
• Abstract ( 1919 ) HTML ( 1524 ) PDF (65KB) ( 1524 ) 　　　
• This study was conducted with leaves collected from the test-tube plantlet of Red Fuji apple as the experimental materials. Changes of hormones，polyamines and NO during in vitro regeneration of adventitious buds were studied under light and dark culture. The results indicated that the contents of ZR，ABA and polyamines under dark culture were higher than that under light culture. The hormone（ZR，ABA and IAA）contents in leaves had the high level during the early stage of inoculation，and the higher level of ZR and ABA，the lower level of IAA were found at the stage of cell division and bud primodium formation. Meanwhile，the ratios of ZR/IAA and ABA/IAA under dark culture were both higher than that under light culture. During the initial stage of inoculation，the endogenous polyamines contents had reached peak，and the contents was also in higher level at the stage of adventitious buds differentiation. During the culture of leaves in vitro，the contents of NO under dark culture kept higher than that of light culture. It is obvious that adventitious bud formation from in vitro leaves of Red Fuji apple was closely associated with contents of endogenous hormones，polyamines and NO.
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• MbNramp1 Gene Cloning，Sequence Analysis and Expression Analysis in Malus baccata（L.）Borkh.
• XIAO Hai-hua;;YIN Li-ping;and HAN Zhen-hai;
• Acta Horticulturae Sinica. 2010, 37(9): 1409-1415.
• Abstract ( 1573 ) HTML ( 1420 ) PDF (623KB) ( 1420 ) 　　　
• Degenerate primers corresponding to the conserved motifs of NRAMP1 in plants were used to amplify specific DNA fragments from Malus baccata（L.）Borkh. roots cDNA. Then the gene specific primers were designed based on the obtained specific DNA fragments，the 3′ end and 5′ end fragments were amplified by RACE. A 2 090 bp full length cDNA MbNramp1 containing a 1 656 bp ORF was obtained based on specific DNA fragments. MbNramp1 encodes a polypeptide of 551 amino acids with a predicted molecular mass of 59.7 kD and a membrane protein with more than 76% non-polar amino acids. All conserved features of NRAMP described previously were present in the predicted MbNRAMP1 sequence，such as putative N-linked glycosylation sites，10 transmembrane domains（TMS）and a consensus transporter motif（CTM）located between Ⅵ and Ⅶ TM. Expression of MbNramp1 was increased in roots under iron deficiency，and its mRNA accumulation patterns differed with the induction time.
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蔬菜

• Pyramiding Disease Resistance Genes by Molecular Marker- Assisted Selection in Tomato
• ZHU Ming-tao;SUN Ya-lin;ZHENG Sha;ZHANG Xiao-li;WANG Tao-tao;YE Zhi-biao;and LI Han-xia;
• Acta Horticulturae Sinica. 2010, 37(9): 1416-1422.
• Abstract ( 2536 ) HTML ( 2446 ) PDF (166KB) ( 2446 ) 　　　
• The elite lines of L11，L19，L46 and L51 containing Tm-2a，I-2，Mi-1 and Cf-9 resistant genes were obtained in tomato with the aid of the molecular marker assisted selection. Seedling inoculation of these inbred lines confirmed higher disease resistant levels. The results of field trials also showed that the four selected disease resistance lines have better agricultural characteristics and may be used for tomato disease resistance breeding program in the future.
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• Recognition of Tomato Foliage Disease Based on Computer Vision Technology
• CHAI A-li;LI Bao-ju;SHI Yan-xia;CEN Zhe-xin;HUANG Hai-yang;and LIU Jun
• Acta Horticulturae Sinica. 2010, 37(9): 1423-1430.
• Abstract ( 2530 ) HTML ( 2611 ) PDF (176KB) ( 2611 ) 　　　
• Computer vision combined with digital image processing and pattern recognition techniques were evaluated for the detection of diseased tomato leaves infected with leaf mold（Fulvia fulva），early blight（Alternaria solani），late blight（Phytophthora infestans），and leaf spot（Corynespora cassiicola）. An image acquisition system was established to acquire leaf images. The image pre-processing techniques were applied to segment the lesion regions from the diseased leaves. And then nine color characteristics，five texture characteristics and four shape characteristics of the lesion regions were extracted. To classify the four kinds of tomato foliage diseases，stepwise discriminant analysis combined with Bayes discriminant analysis and principal component analysis combined with Fisher discriminant analysis were executed to develop the discriminant models. By the stepwise discriminant analysis，we selected 12 characteristics from the original 18 variables to develop the Bayes discriminant function, and results showed that the classification accuracies for the training and testing sets achieved 100% and 94.71% respectively. By principal component analysis，the 18 variables were reduced to two principal components（PCs）. The classification model based on the two PCs achieved classification accuracy of 98.32%. These results indicated that it is feasible to identify and classify tomato diseases using computer vision technology. This preliminary study, which was done in a closed room with restrictions to avoid interference of the field environment, showed that there is a potential to establish an online field application in tomato diseases detection based on computer vision and image processing techniques.
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• Cryopreservation of In Vitro Shoot Tips of Potato by Droplet Vitrification and Genetic Stability of Regenerated Plantlets
• BAI Jian-ming;;CHEN Xiao-ling;LU Xin-xiong;GUO Hua-chun;XIN Xia;ZHANG Zhi-e;and XIN Ping-ping
• Acta Horticulturae Sinica. 2010, 37(9): 1431-1438.
• Abstract ( 2194 ) HTML ( 1434 ) PDF (157KB) ( 1434 ) 　　　
• Shoot tips from axillary buds of in vitro potato（Solanum tuberosum L.）were successfully cryopreserved by droplet vitrification. The optimum cryopreservation procedures were as follows. Shoot tips excised from 2–3 month-old plantlets were precultured on liquid MS medium supplemented with 0.3 mol · L-1 and 0.5 mol · L-1 sucrose for 1 day each and then dehydrated with PVS2 for 30 min at 0 ℃. Five shoot tips were transferred to approximately 15 µL droplets of PVS2 solution on thin strips of sterile aluminum foil. The aluminum foil strips were folded to enclose the shoot tips. The foil envelope was then carefully immersed into liquid nitrogen（LN）using fine forceps. After immersion the strips were quickly transferred to 2 mL cryotubes and immediately plunged into LN and maintained for 1 h at least. The shoottips in the foil strips were then rapidly heated by immersion twice at 15 min intervals into 10 mL 1.2 mol · L-1 sucrose MS medium at room temperature. The shoot tips were then unloaded from the foil，rinsing the PVS2 from the shoot tips. The shoot tips were transferred to solid culture medium（MS + 0.5 mg · L-1 Zeatin + 0.1 mg · L-1 NAA + 1.0 mg · L-1 GA3）for recovery and regeneration. The average survival rate and regeneration rate were 79.91% and 62.52%，respectively. There was no genetic variation in the regenerated plants based on assessment of SSR markers.
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• Moderate Water Stress Increased the Photosynthesis Adaptability of Cucumber Seedlings Under Low Light
• XIAO Wen-jing;SUN Jian-lei;WANG Shao-hui;SUN Hui-hui;WANG Hong-yun;SUI Xiao-lei;and ZHANG Zhen-xian;
• Acta Horticulturae Sinica. 2010, 37(9): 1439-1448.
• Abstract ( 2332 ) HTML ( 1616 ) PDF (188KB) ( 1616 ) 　　　
• The effects of water stress（40% maximum moisture capacity）on the photosynthetic and fluorescent characteristics and the content of Rubisco of Cucumis sativus‘Deltastar’seedlings under low light（75–85 μmol · m-2 · s-1）were investigated in a phytotron. The results revealed that after 20 days of water stress under low light，the photosynthesis pigment contents per unit area of leaves of cucumber increased and the values of Chl.a/b decreased. The values of light saturated rate of the CO2 assimilation（Asat），apparent quantum yield（AQY），carboxylation efficiency（CE），maximum RuBP regeneration rate，light saturation point（LSP）increased significantly but CO2 compensation point（CCP）decreased remarkably. The chlorophyll fluorescence parameters including PSⅡ maximal photochemical efficiency（Fv/Fm），PSⅡ potential photochemical efficiency（Fv/Fo），PSⅡ actual photochemical efficiency（ΦPSⅡ），the photochemical reaction rate（Prate），the linear whole-chain electron transport rate（J）and the partitioning proportion for photochemical reaction of light energy absorbed by PSⅡ（P）showed increased trend，but the imbalance of partitioning of excitation energy between PSⅠand PSⅡ（β/α–1） decreased. The content of soluble protein and the content of Rubisco increased. These results indicated moderate water stress increased the photosynthesis adaptability of cucumber seedlings under low light. However，after 20 days of water stress under normal light（500–600 μmol · m-2 · s-1），the values of the items including Asat，maximum RuBP regeneration rate，CE，ΦPSⅡ，Prate and P decreased. But the values of（β/α–1） increased. So the photosynthesis of cucumber under normal light was inhibited by water stress.
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• Mapping QTLs for Multiple Pistillate Flowers in Cucumber
• MIAO Han;GU Xing-fang;ZHANG Sheng-ping;WU Jian;FANG Zhi-yuan;and ZHANG Zhen-xian
• Acta Horticulturae Sinica. 2010, 37(9): 1449-1455.
• Abstract ( 2049 ) HTML ( 2266 ) PDF (163KB) ( 2266 ) 　　　
• Multiple pistillate flowers trait is related closely to cucumber（Cucumis sativus L.）production. This study was made to map and analyze QTLs of multiple pistillate flowers traits in cucumber，so as to provide information for molecular marker assisted selection of cucumber breeding for high yield. A linkage map of cucumber was constructed using 148 F9 recombinant inbred lines（RILs）originated from a narrow-cross between 9110Gt（single pistillate flower，SP）and 9930（multiple pistillate flowers，MP）in the experiment. Total of 8 QTLs were detected using the genetic map and multiple QTL model（MQM）method of software package MapQTL version 4.0. These QTLs were mapped on chromosome 1，2，3，6，7，respectively. Their LOD values varied between 3.62 and 8.37 which explaining 8.2%–20.0% of the phenotypic variation. Six QTLs explained phenotypic variation more than 10% which Mp3.2 explained highest phenotypic variation（2007 autumn 20.0%，LOD = 8.37），and Mp6.2（2006 spring 13.9%，LOD = 5.95；2007 autumn 11.9%，LOD = 5.10；2009 spring 11.5%，LOD = 4.38）was detected in the two seasons. The tight linkage markers（SSR04635，SSR13466，SSR00584 and SSR10449）can be used in fine mapping of multiple pistillate flowers in cucumber.
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观赏植物

• Karyotype Features of 17 Species of Spiraea and Karyotype Parameters Analysis
• LIU Hui-min;CHEN Ya-Jun;LÜGui-e;LI Li;and WU Feng-zhi;
• Acta Horticulturae Sinica. 2010, 37(9): 1456-1462.
• Abstract ( 2091 ) HTML ( 1745 ) PDF (138KB) ( 1745 ) 　　　
• 17 species of Spiraea were selected as materials，using wall degradation hypotonic method to analyse the features of chromosome，to do associativity analysis and cluster analysis，to discuss the karyotype features and macroevolution. The results suggested that the 17 species of Spiraea had stable chromosome number，2n = 18 and 2n = 36，basic number of chromosome was 9，their chromosomes were mainly composed by median region chromosome（m）and submedian region chromosome（sm），in which there were satellites in Spiraea dasyantha，Spiraea chamaedryfolia and Spiraea canescens. Their karyotype types were 1A，1B，2B，their average arm ratio changes from 1.26 to 1.64，Lt/St changes from 1.67 to 3.51，centromeric index changes from 38.96% to 44.26%，A.s.k% changes from 56.14% to 60.69%. We sorted the evolution degree of 17 species of Spiraea from high to low according to the karyotype dissymmetry coefficient，in which S. media，S. dasyantha and S. canescens were high，S. thunbergii，S. chamaedryfolia and S. trilobata were low. The importance of karyotype parameters was A.s.k% > A.A.R > P.C.A% > Lt/St > karyotype type > centromeric index. There were differences of karyotype parameters in 17 species of Spiraea，karyotype datas could provide a base for the classification of Spiraea. Seventeen species of Spiraea could be divided into 4 groups by karyotype features cluster.
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• Comparative Studies on Trichomes of Florist’s Chrysanthemum and Pyrethrum
• LI Jie;XING Mei;LI Ya-fei;JONGSMA Maarten;ZHANG Die;and WANG Cai-yun;
• Acta Horticulturae Sinica. 2010, 37(9): 1463-1470.
• Abstract ( 2455 ) HTML ( 1718 ) PDF (185KB) ( 1718 ) 　　　
• The trichome type，density and color on the epidermis of leaves and flowers of florist’s chrysanthemum and pyrethrum were investigated utilizing Scanning Electron Microscopy（SEM）and fluorescence microscopy. Capitate glandular trichomes and T-shaped non-glandular trichomes were found on both species. The capitate glandular trichomes were distributed on both sides of the leaves，and the corolla of disc florets，but only on the abaxial surface of ray florets of florist’s chrysanthemum. In the case of pyrethrum they were also found on both sides of leaf surfaces and disc floret corollas，and in this case on the opposite adaxial surface of ray florets. The highest density of capitate glandular trichomes was detected on the surface of ovaries of pyrethrum. Remarkably they were few or not observed on chrysanthemum ovaries. The T-shaped non-glandular trichomes were distributed on the epidermis ofleaves，involucre and stalks of both species，but none on the ray and disc florets. Under UV light capitate glandular trichomes were blue on leaves of both species，but on the disc and ray florets of pyrethrum a few green ones were observed. The fluorescence color of the capitate glandular trichomes on the ovaries changed from red to blue with the development of the pyrethrum flower. By contrast，the T-shaped non-glandular trichomes were yellow under blue fluorescence of both species，and it appeared blue under UV light. The relationships between trichome differences and insect resistance were discussed.
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• Establishment of Plantlet Regeneration System of Tree Peony Through Lateral Buds Cutting and Carving
• LIU Hui-chao and JIA Wen-qing
• Acta Horticulturae Sinica. 2010, 37(9): 1471-1476.
• Abstract ( 2078 ) HTML ( 1346 ) PDF (73KB) ( 1346 ) 　　　
• Tree peony‘Wulong Pengsheng’s lateral buds were used as explants. It was the first time to study the effect of cutting lateral buds on the adventitious buds differentiation. The influence of plant growth regulation on the adventitious buds induction，the multiplication and rooting was also studied. The results were as followed：（1）Disinfecting with 75% alcohol for 30 s，and then soaking with 0.1% corrosive sublimate for 7 min had good effect of disinfectation；（2）Compared with the control，the adventitious buds induction rate significantly increased through cutting，and the number of adventitious buds increased by 2.6–4.8 times，with the maximum reaching 96%；（3）The best medium for proliferation was WPM + 6-BA mg · L-1 + IAA 0.2 mg · L-1，with the proliferation of was 787.05%；（4）The rate of plantlets rooting was up to 81.33% on 1/2 WPM medium supplemented with NAA 1.0 mg · L-1 and IBA 4.0 mg · L-1. The plantlets were successfully transplanted to potting and the survival rate of transplanted plantlets was 90%.
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• Cloning，Expression and Subcellular Localization of P5CS Gene from Perennial Ryegrass（Lolium perenne L.）
• CAO Li;SUN Zhen-yuan;YI Ming-fang;HAN Lei;and XIN Hai-bo
• Acta Horticulturae Sinica. 2010, 37(9): 1477-1484.
• Abstract ( 1882 ) HTML ( 2500 ) PDF (250KB) ( 2500 ) 　　　
• In this study，full length cDNA sequence of a putative Δ1-pyrroline-5-carboxylate synthase gene（LpP5CS）was cloned from perennial ryegrass（Lolium perenne L.‘Derby’）leaves using RT-PCR and rapid amplification of cDNA ends（RACE）. Sequence analysis showed that the nucleotide sequence of this gene is 2 528 bp，containing a complete open reading frame and encoding 716 amino acids. Nucleotide and amino acid sequence analysis revealed that LpP5CS shares high identity with the orthologs from Triticum aestivum（92.05%，93.99%）and Oryza sativa（85.82%，87.99%），respectively. Semi-quantitative RT-PCR analysis showed that LpP5CS expressed in different tissues. Various elevated levels of LpP5CS expression have been detected when the seedling roots exposed to high salinity. Tested with salt solution，its expression was higher than control，and the highest in leaves，the lowest in roots，The contents of LpP5CS increased first then decreased with the processing time is extended after 200 mmol · L-1 NaCl treatment. Finally，subcellular localzation assays showed that the LpP5CS protein was present in the nucleus and at the plasmalemma.
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• Cloning and Expression Characterization of the Cellulose Synthase Gene （PeCesA）from Moso Bamboo（Phyllostachys edulis）Shoot
• ZHANG Zhi-jun;YANG Yang;HE Sha-e;LUO Shu-ping;and LIU Zhi-wei
• Acta Horticulturae Sinica. 2010, 37(9): 1485-1492.
• Abstract ( 1747 ) HTML ( 1695 ) PDF (232KB) ( 1695 ) 　　　
• The cellulose synthase gene（CesA）plays a key role in regulating cellulose biosynthesis. A 3 545 bp cDNA clone encoding PeCesA（GenBank accession No. FJ799713）was isolated from the full- length cDNA library of the shoot of Moso bamboo（Phyllostachys edulis）by PCR screen. The cDNA had an 3 243 bp open reading frame（ORF）which would be capable to encode 1 081 amino acid residues. Multiple alignment analyses showed that the PeCesA shared high similarity（over 90%）with the CesA genes from Bambusa oldhamii，Oryza sativa，Triticum aestivum and Hordeum vulgare. And the deduced amino acid sequence of PeCesA contained highly conserved two Zinc-binding domains，eight transmembrance domains and one QVLRW motif，which were special characteristics among the cellulose synthase of plant. Phylogenetic analysis showed that PeCesA was clustered in the group that included CesA4s from Bambusa oldhamii，Oryza sativa and Zea mays respectively. The expression profile of PeCesA was analyzed by quantitative Real Time PCR. The results showed that the expression of PeCesA in the top of the bamboo shoots was extremely low，but it was high in the base of shoot. And it was relatively lower in the leaves compared to the roots and stems. The results of determination of cellulose content showed there was correlation between the expression of PeCesA and the cellulose content in different organisms. It suggested that PeCesA involved in cellulose synthesis in second cell wall Moso bamboo shoots.
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研究报告

• Effects of Cool and Cold Conditions on Nitrogen Uptake Kinetics in Malus baccata Borkh. Seedlings
• Lü De-guo;WANG Ying;QIN Si-jun;MA Huai-yu;LIU Guo-cheng;DU Guo-dong;and MENG Qian
• Acta Horticulturae Sinica. 2010, 37(9): 1493-1498.
• Abstract ( 1739 ) HTML ( 1406 ) PDF (72KB) ( 1406 ) 　　　

• The method of conventional exhaustion and improved exhaustion was carried out to study the kinetics characteristics of nitrogen absorption in root of Malus baccata Borkh. seedlings in cool and cold conditions. The characteristics of nitrogen absorption kinetics were affected by cool and cold condition. In normal temperature（25 ℃）the affinity of NO3- was greater than that of NH4+ which indicated that root had the priority to absorb NO3-. But in cool and cold condition（10 ℃）the affinity of NH4+ was greater than that of NO3-，and in this moment the root had the priority to absorb NH4+. The M. baccata Borkh. seedlings’ absorption was decreased in cool and cold condition and it was indicated that the maximum absorption velocity，nutrient inflow velocity and affinity of NO3- and NH4+ were decreased in cool and cold condition. The influence of cool and cold condition in absorbing NO3- was greater than absorbing NH4+.

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• Analysis of Castanea mollissima Germplasm Resources by AFLP
• LAN Yan-ping;ZHOU Lian-di;YAO Yan-wu;WANG Shang-de;and LIU Guo-bin
• Acta Horticulturae Sinica. 2010, 37(9): 1499-1506.
• Abstract ( 1674 ) HTML ( 1506 ) PDF (87KB) ( 1506 ) 　　　
• The genetic variation and genetic relationships of 90 Castanea mollissima cultivars were analyzed by the fluorescent-AFLP technique with 9 primer combinations. Among the 806 bands detected，695 bands were polymorphic，and the high percentage of polymorphic loci（86.23%）revealed a high genetic diversity among the 90 C. mollissima cultivars. The UPGMA phenogram based on genetic identity clustered the 90 C. mollissima cultivars into 2 groups and 14 rows. The 9 pairs of AFLP primer combination used could distinguish all the tested cultivars，the highest ratio of identification is 93% and the lowest is 80%.
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• Effects of Propylene and 1-Methylcyclopropene on PG Activities and Expression of DkPG1 Gene During Persimmon Softening Process
• JIANG Ni-na;RAO Jing-ping;FU Run-shan;and SUO Jiang-tao
• Acta Horticulturae Sinica. 2010, 37(9): 1507-1512.
• Abstract ( 1850 ) HTML ( 1707 ) PDF (122KB) ( 1707 ) 　　　

• In order to further explore the molecular regulation mechanism of PG during‘Fuping Jianshi’persimmon（Diospyros kaki L.）postharvest softening with different treatments, and to improve the persimmon ripening and softening process，the responses of DkPG1 gene and PG activities in persimmon fruit to propylene and 1-MCP at the room temperature were investigated by real-time quantitative PCR（RT-qPCR）approach and DNS method. The results showed that accompanying with fruit ripening，the expressions of DkPG1 gene in fruits with different treatments had the same trend on the transcript level that it first steadily increased to the maximum and then decreased. After 4 days of propylene treatment，the expression of DkPG1 gene increased to the maximum which was dramatically higher than the control，and it appeared 12 days ahead of the control；The expression peak of DkPG1 gene in fruits treated with 1-MCP appeared at 28 days，12 days later than the control，which was significantly lower than the control. The activity of PG in fruits treated with propylene rapidly increased, and the activity peak appeared at the same day DkPG1 gene expression peak appeared；1-MCP treatment made the activity peak of PG decrease and appear 4 days earlier than its DkPG1 expression peak. Besides，the results about the analyze of ethylene release rate and pectin component showed the expressions of DkPG1 gene were regulated by ethylene，and then affected the pectin component metabolism.

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• Screening and Analysis of BAC Clones Containing Flowering Time Gene FLC1 in Brassica campestris L. ssp. pekinensis Olsson
• SHI Xue-ping;FENG Da-ling;WANG Yan-hua;XUAN Shu-xin;and SHEN Shu-xing
• Acta Horticulturae Sinica. 2010, 37(9): 1513-1516.
• Abstract ( 2218 ) HTML ( 2322 ) PDF (62KB) ( 2322 ) 　　　
• The primary and secondary pools of a bacterial artificial chromosome（BAC）library in Chinese cabbage were constructed using improved BAC pooling strategy. The specific primers of flowering time gene FLC1 were to screen those BAC clones by PCR amplification. In total two positive FLC1 clones were obtained from 19 200 BACs through three steps of PCR screening with 114 reactions. Sequence analysis of FLC1 clones showed that these fragments has 99% of homology with the FLC1 in Chinese cabbage，indicating that the flowering time gene FLC1 is contained in the BAC clones.
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• Responses of Phyllitis japonica to Shading Treatment
• YUE Hua and YUE Xiao-jing
• Acta Horticulturae Sinica. 2010, 37(9): 1517-1522.
• Abstract ( 1651 ) HTML ( 1396 ) PDF (78KB) ( 1396 ) 　　　
• The effects of all optical，30%，50%，70% and 90% shading treatments on the growth and photosynthetic characteristics of Phyllitis japonica were studied. The results showed：With the increase of light intensity，the leaf area，fresh weight of unit leaf area，chlorophyll content，and leaf water content decreased and soluble sugar and soluble protein content declined. The diurnal variation of net photosynthetic rate（Pn）presented the double-peak curve，indicating there was midday depression of photosynthesis; the optimum treatment to diurnal average Pn was 50%–70% shading. The light compensation point（LCP）reduced gradually and the light saturation point（LSP）was the highest under 50% shading，which was lower in 70% and 90% shading. Comprehensively，the suitable light environment of Phyllitis japonica was 50%–70% shading.
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综述

• Advances in Researches on Ornamental Plant Growth Simulation Model
• TAN Mei and WANG Si-qing
• Acta Horticulturae Sinica. 2010, 37(9): 1523-1530.
• Abstract ( 1712 ) HTML ( 1523 ) PDF (54KB) ( 1523 ) 　　　
• Crop growth model is a powerful tool in crop production precise management and intelligent decision-making. This paper presented a review on domestic and foreign research progresses of ornamental plant growth simulation model，respectively from the growth simulation（leaf area，photosynthesis，dry matter production and distribution），development simulation and quality simulation three aspects to carry on the summary and discussion，and finally pointed out the problems existing in ornamental plant growth simulation model research and the future development direction.
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新品种

• A New Middle-late Ripening Apple Cultivar‘Jinxiu Hong’
• YAN Zhen-li;ZHANG Heng-tao;GUO Guo-nan;ZHANG Shun-ni;and LIU Zhen-zhen
• Acta Horticulturae Sinica. 2010, 37(9): 1531-1532.
• Abstract ( 1895 ) HTML ( 1698 ) PDF (613KB) ( 1698 ) 　　　
• ‘Jinxiu Hong’is a new middle-late ripening apple cultivar，selected from mutation breeding of‘Huaguan’. Its fruit shape is nearly conical，with a greenish yellow skin，covered with full red blush. The average fruit weight is 205 g. The flesh is light yellow in color，fine，crisp and juicy，sour-sweet palatable，with a firmness of 18.06 kg · cm-2，13.3% soluble solids content，11.67% total sugar，0.29% titratable acid content，0.0514 mg · g-1 vitamin C content. It has very good quality and excellent storage ability. The trees are precocious and very productive. It matures 15 days earlier than ‘Huaguan’in the middle of September.
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• A New High Quality Mid-late Maturing Pear Cultivar‘Jiyu’
• LI Xiao;HAN Yan-xiao;WANG Jing-tao;LI Yong;LIU Guo-sheng;and WANG Ying-wao
• Acta Horticulturae Sinica. 2010, 37(9): 1533-0534.
• Abstract ( 1597 ) HTML ( 1420 ) PDF (2125KB) ( 1420 ) 　　　
• The new pear cultivar‘Jiyu’is the progeny of hybrid‘Xuehua Pear’בCuiyun Pear’. The fruit ripens in later August or early September，with oval in shape and green-yellow skin. The average fruit mass is 260 g. The fruit fresh is fine and crisp with tart-sweet fragrance. The soluble solids content is 12.3%. The cultivar has a top grand quality. It also has other characteristics such as high yield and resistance to pear scab.
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• A New Cold Resistant High Quality Pear Cutivar‘Hanluli’
• ZHANG Mao-jun;DING Li-hua;WANG Qiang;YAN Xing-kai;and XING Guo-jie
• Acta Horticulturae Sinica. 2010, 37(9): 1535-1536.
• Abstract ( 1574 ) HTML ( 1265 ) PDF (458KB) ( 1265 ) 　　　
• ‘Hanluli’，tested as 77-23-272，is a new，high quality pear cultivar released by Jilin Academy of Agricultural Sciences in 2010，which was derived from‘Yanbian Daxiangshui’（P. ussuriensis Maxim）בHangqing’（P. pyrifolia），with the higher hardy and disease resistance. The fruit is short cone-shaped. The average fruit mass is 220 g. The peel is yellow-green. The flesh is fine，crisp，juicy，sour-sweet，with little stone cell and aroma. The soluble solids content is 14%. The soluble sugar content is 9.01%. The titratable acid content is 0.20%，and the vitamin C content is 0.0068 mg · g^-1.
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• A New Chestnut Cultivar‘Dongwangmingli’
• SHEN Guang-ning;MING Gui-dong;TIAN Shou-le;XU Lin;LIU Xu;and SHAN Gong-hua
• Acta Horticulturae Sinica. 2010, 37(9): 1537-1538.
• Abstract ( 1734 ) HTML ( 1127 ) PDF (777KB) ( 1127 ) 　　　
• ‘Dongwangmingli’is a new chestnut cultivar selected form seedlings. It shows goog characteristics such as early fruiting and high yield. The nut is glossy，large size，with good quality. The cultivar is suitable for cultivation in the Tai-yi mountains region in central Shandong，and flood land in the Southeastern of Shandong Province.
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• A New Tetraploidy Table Chinese Jujube Cultivar‘Chenguang’
• LIU Meng-jun;LIU Ping;JIANG Hong-en;DAI Li;WU Gai-e;and LIU Zhi-guo
• Acta Horticulturae Sinica. 2010, 37(9): 1539-1540.
• Abstract ( 2015 ) HTML ( 1541 ) PDF (855KB) ( 1541 ) 　　　

• ‘Chenguang’is a new tetraploidy Chinese jujube（Ziziphus jujuba Mill.） cultivar obtained from‘Linyilizao’which was induced by colchicine. Its fruit is big，nearly round，with the average fruit weight of 39.6 g and edible ratio of 98.5%. The fruit tastes sweet，crisp and juicy，with thin pericarp. ‘Chenguang’is characterized by high yield，early-fruiting. It is an excellent mid-rippening table cultivar which is suitable to be cultivated in mid-south part of Hebei Province.

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• A New Strawberry Cultivar‘Shu Xiang’
• ZHANG Yun-tao;WANG Gui-xia;DONG Jing;ZHONG Chuan-fei;WANG Li-na;and CHANG Lin-lin
• Acta Horticulturae Sinica. 2010, 37(9): 1541-1542.
• Abstract ( 1982 ) HTML ( 1328 ) PDF (769KB) ( 1328 ) 　　　
• ‘Shu Xiang’is a new strawberry cultivar bred from the cross‘Nyoho’בDarselect’. The fruit is cone-shaped and uniform. The average fruit weight is 24.5 g，and the maximum is 76 g. It has good color，excellent flavor，high firmness，bright luster，tolerance to storage and transport，resistance to powdery mildew. It has 10.9% soluble solids content，5.56% total sugar content，0.52% total acid content，0.492 mg · g-1 vitamin C content，and 2.293 kg · cm-2 firmness. It ripens in early-mid January in the greenhouse in Beijing.
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• A New Green Radish Cultivar‘Wei Luobo 3’
• HAN Tai-li;TAN Jin-xia;YANG Xiao-dong;XU Li-gong;and SONG Yin-hang
• Acta Horticulturae Sinica. 2010, 37(9): 1543-1544.
• Abstract ( 1619 ) HTML ( 1522 ) PDF (917KB) ( 1522 ) 　　　
• ‘Wei Luobo 3’is a new radish cultivar which derived from‘VL01-04’בVL03-121’. It is of high quanlity，yield and good adaptability. It has 10–12 dark green leaves serrated，with smooth succulent root. The root is cylindrical，with green skin. The average single fruit weight is about 1.5–2.0 kg. The yield is 64.20–80.05 t · hm-2. It takes 80 days from sowing to maturation. It has resistance to downy mildew，virus diseases and soft rot disease. It is suitable for fall planting in Shandong Province and Northeast China.
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• A New Rosa Cultivar‘Honey’
• WANG Qi-gang;ZHANG Hao;JIAN Hong-ying;LI Shu-fa;QIU Xian-qin;ZHANG Ting;YAN Hui-jun;TANG Kai-xue;and WANG Ji-hua
• Acta Horticulturae Sinica. 2010, 37(9): 1545-1546.
• Abstract ( 2094 ) HTML ( 1794 ) PDF (2034KB) ( 1794 ) 　　　
• ‘Honey’is a new cut rose hybrid developed by crossing female parent‘Hollywood’× male parent‘Laser’. It is vigrous and progenitive. The length of stem is 70–90 cm and its thickness is uniform. The flower is high-heart，wide large，with light fragment. The flower is yellow-white with a little pink，inside and outside petal color are identical.
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