研究论文

• Effects of Water Stress on Nucleic Acid Metabolisms and Changes of Free Radicals in Malus
• Cao Hui;Han Zhenhai;and Xu Xuefeng
• Acta Horticulturae Sinica. 2002, 29(6): 505-509.
• Abstract ( 1416 ) HTML ( 1283 ) PDF (119KB) ( 1283 ) 　　　
• Effects of water stress on nucleic acid metabolisms and changes of free radicals in Malus hupehensis pamp Reld. and Malus sieversii (Ledeb) Roem. seedings were studied with polyethylene glycol (PEG6000) treatment . Under water stress , the contents of RWC , DNA and RNA in leaves decreased , while the content of O₂^-· and H₂O₂, the activities of DNase and RNase increased. Compared with RNA , the less decrement of DNA content was
  accompanied with higher activity of DNase. The changing extents in RWC , active oxygen , DNA , RNA , DNase and RNase in the drought tolerant variety M. sieversii were lower than the drought resistant variety M. hupehensis. Under water stress , there are high significant correlation between DNA , RNA content and DNase , RNase , RWC ,O₂^-·, H₂O₂ in M. hupehensis pamp Reld. and M. sieversii (Ledeb) Roem. seedings.
• Related Articles | Metrics

• Interaction between AM Fungi and Grape Meloidogyne incognita
• Li Haiyan;Liu Runjin;and Shu Huairui
• Acta Horticulturae Sinica. 2002, 29(6): 510-514.
• Abstract ( 1601 ) HTML ( 1399 ) PDF (213KB) ( 1399 ) 　　　
• The interactions between arbuscular mycorrhizal (AM) fungi Glomus versiforme Berch , Gigaspora margarita Becker & Hall , Glomus mosseae Nicolson & Gedermann and Meloidogyne incognita on grape ‘Kyoho’
  were studied under the condition of pot culture in a greenhouse. The results showed that the tested AMfungi were able to inhibit the generation and development of M. incognita , decrease the number of the second2stage juveniles in the soil of root zone , the diameter of egg mass , the number of eggs in per egg mass and on per gram root ; In addition , the treatment of pre2inoculating AM fungi and post2inoculating M. incognita performed more effectively on grape’s resistance to M. incognita than that inoculating AM fungi and M. incognita concurrently ; Inoculating AMfungi promoted plant’s photosynthesis , increased plant hight , stem diameter , leaf area , dry mass of tops or roots , which compensated the damage produced by M. incognita ; Inoculating M. incognita decreased the number of vesiculars and entry points number of G. mosseae , as well as the colonization percent of G. versiforme and Gi. margarita , while increased the number of vesiculars and entry points of G. versiforme and Gi . margarita. Therefore , we can not give a common conclusion to the every association of AMfungi-plant-nematode.
• Related Articles | Metrics

• Effects of Irrigation on Grape and Wine
• Zhang Zhenwen;Li Hua;and Song Changbing
• Acta Horticulturae Sinica. 2002, 29(6): 515-518.
• Abstract ( 1276 ) HTML ( 1262 ) PDF (220KB) ( 1262 ) 　　　
• The effects of different irrigation methods on growth of vine and quality of grape and wine were studied. The results showed that the soil configurationwas improved by controlled irrigation, with increasing of root density in deep soil and sugar content of berry. Meanwhile the acid and salt content of the berry decreased and pH was modified. The wines had better aroma and flavor under controlled irrigation. 3825- 4800 m3#hm^-2 water per year was needed in controlled irrigation, saving 36% water compared to the regular one.
• Related Articles | Metrics

• Studies on the Interspecific Hybridization in the Genus Fragaria
• Lei Jiajun;Dai Hanping;Deng Mingqin;Wu Luping;and Hu Wenyu
• Acta Horticulturae Sinica. 2002, 29(6): 519-523.
• Abstract ( 1977 ) HTML ( 1965 ) PDF (218KB) ( 1965 ) 　　　
• The interspecific hybridization was carried out with diploid , tetraploid , pentaploid , octoploid species in the genus Fragaria as materials. The percentage of fruit2setting was high when the ploidy level of both
  parents was same , e. g. , 53. 3 % in the cross of F. viridis (2x) and F. mandschurica (2x) . While the ploidy of both parents was different , the fruit-setting depended to a great extent on whether the ploidy of the female parent was higher than that of the male. If the lower ploidy species was used as the female parent , the seeds would not or hardly be obtained. On the contrary , if the higher ploidy species was used as the female parent , the percentage of setting was relatively high , e. g. , 68. 4 % in the cross of cv. Yuhime (8x) and natural wild line Heilongjiang No. 7 ( 5x) . One nonaploid seedling was obtained from the corss of Yuhime and Heilongjiang No. 7. The
  unreduced gamete was postulated according to its ploidy , meioses and the size of pollens of pollens of parents. The seedlings from interspecific hybridization varied greatly. Some maternal seedlings which were assumed to have resulted from apomixis occurred in several cross combinations. Diploid , tetraploid , pentaploid , hexaploid , heptaploid , octoploid , nonaploid seedlings were obtained from the interspecific hybridization , which was helpful to improve the quality , stress and disease resistance of the cultivated strawberry , and also valuable for research in strawberry genetics.
• Related Articles | Metrics

• Effects of Delaying and Shortening the New Low Oxygen CA CombinationTreatment Time on the Storage of Apples
• Yuan Kejun;Li Zhensan;Zhang Daohui;Sun Yugang;Zhang Dapeng;and Hu Xiaosong
• Acta Horticulturae Sinica. 2002, 29(6): 524-528.
• Abstract ( 1401 ) HTML ( 1059 ) PDF (112KB) ( 1059 ) 　　　
• We formerly reported a special lowoxygen CA combination (2 %O₂ + 7. 5 %CO₂) , which can control superficial scald of apples for a longer period after 4 months of treatment . Now , a further experiment of Starkrimson apples was conducted to study the effects of delayed and shortened treatment . The results showed that the scald incidence of apples of control (0 ℃, air) and conventional CA treatment (0 ℃, 3 %O₂ + 3 %CO₂) were 25. 6 % and 1. 02 % respectively , and their flesh firmness were 5. 79 and 6. 52 kg/ cm2 respectively after 123 days of storage. The apples of special CA treatment (10 ℃, 2 %O₂ + 7. 5 %CO₂) was scald2free after 123 days of storage , its flesh firmness (6. 63 kg/ cm²) was the same as that of conventional CA treatment and significantly higher than that of control . The apples of delayed treatment developed scald (6. 67 %) after 15 days of storage at 10 ℃in
  air and 108 days of special CA treatment , its flesh firmness (4. 8 kg/ cm²) was significantly higher than those of control and conventional CA treatment . These results indicted that it is necessary to treat apples with special CA
  combination on time. The scald incidence of apples of control , conventional CA treatment and special CA treatment were 46. 9 % , 36. 4 % and 0 respectively after 123 days of storage plus another 58 days of cold storage in air , their flesh firmness were 5. 28 , 5. 21 and 4. 5 kg/ cm² respectively. The apples of special CA treatment was also scald free after 96 days of storage plus another 50 days of cold storage in air , its flesh firmness was 4. 67 kg/ cm². Based on the consideration of both scald incidence and flesh firmness , it is possible to change the duration of special CA treatment from 4 months to 3. 3 months if we want to store apples for about 5 months. The formations ofα2farnesene and conjugated trienes in the peels of apple fruits were significantly inhibited by special CA treatment during CA storage.
  
• Related Articles | Metrics

• Inheritance of High Temperature Tolerance in Eggplant (Solanum melongenaL.)
• Yi Jinxin;and Hou Xilin
• Acta Horticulturae Sinica. 2002, 29(6): 529-532.
• Abstract ( 1397 ) HTML ( 1189 ) PDF (184KB) ( 1189 ) 　　　
• The inheritance of high temperature tolerance (HT) in eggplant was studied with 4 ×4 half-diallel crosses, which derived from four parents with different tolerance to high temperature. Two parents and their progeny of F₁ , F₂ and BC₁ involved in present experiment were simultaneously treated by high temperature in greenhouse and open field. HT index and ratios of fruit setting were respectively collected following Hayman’s procedure. The results showed that inheritance of HT in eggplant is of incomplete dominance that fits in with a model of additive-dominance , and additive effects play more important roles on HT than dominant effects do. Furthermore , selecting methods for improving HT of eggplant were suggested.
• Related Articles | Metrics

• A Study on the Correlation Between Several Chemical Substances and Capsicum Cultivar Resistance to Myzus persicae (Sulzer)
• Chen Qing
• Acta Horticulturae Sinica. 2002, 29(6): 533-536.
• Abstract ( 1286 ) HTML ( 1247 ) PDF (142KB) ( 1247 ) 　　　
• The Capsicum cultivars that had been showed steadily resistant to Myzus persicae (Sulzer) were used. This study was proceeded in 4- 6􀀁leaf stage to determine the contents of amino acids, nitrogen, sugar, proline and vitamin C in leaves. The results showed that the contents of amino acids, nitrogen, sugar, proline, vitamin C and the ratio of sugar and nitrogen were significant difference between resistant and suscept ible cultivars. Resistant cultivars had less amino acids, nitrogen, sugar, proline and vitamin C but more sugar and high ratio of sugar and nitrogen than susceptible ones; the contents of amino acids, nitrogen, sugar, proline and vitamin C of the later were 1. 1- 2. 9 times, 1. 0- 1. 7 times, 1. 4- 2. 9 times and 1. 2- 2. 6 times as much as those of the former, but the contents of sugar and the ratio of sugar and nitrogen of the former were 1. 1- 1. 3 times and 1. 3-2. 0 times as much as those of the later. The extremely signif icant difference between high resistant and high susceptible cultivars indicated that the contents of amino acids, nitrogen, sugar, proline, vitamin C and the rate of sugar and nitrogen were significantly relative to the resistance of the Capsicum cultivars to Myzus persicae(Sulzer) .
• Related Articles | Metrics

• The Changes of Several Endogenous Phytohormones during Microtuber Formation in vitro in Solanum tuberosum L.
• Lian Yong;Zou Ying;Dong Huiru;Jin Liping;and Lin Huan
• Acta Horticulturae Sinica. 2002, 29(6): 537-541.
• Abstract ( 1574 ) HTML ( 1234 ) PDF (178KB) ( 1234 ) 　　　
• Virus free platelets in vitro of potato cv. Favoritawere used for the experiments. The changes of en dogenous phytohormones GA₃, IAA, KT, BAP, ABA during microtuber formation in vitro were invest igated. The
  results showed that ( 1) overall, endohormone content in roots was higher than that in shoots during the period from platelets culture to microtuber formation; ( 2) Endohormone contents varied at all stages of microtuber format ion, GA₃, IAA, KT, BAP content in platelets and ABA content in the roots at initial stage of microtuber induction ( after transfering to inducation medium and under light conditions for 2 days) was higher than that at the stage of platelet culture. GA₃, IAA , KT, BAP content in the roots and IAA content in the shoots were reduced at the stage of stoloniferous shoots development ( 3 days under dark condit ions) but ABA content and IAA content in the root had no obvious changes. GA3, KT and BAP content in the shoots risen again and GA3 and BAP content in the root continuously reduced at the stage of microtuber formation ( 7 days under dark conditions) . IAA and BAP content increased gradually, BAP in the roots, KT in shoots reduced, KT in the roots and GA₃ in the shoots were stable at the stage of microtuber development ( 14 days under dark conditions) .
• Related Articles | Metrics

• Studies on Methods and Mechanisms of Improving Vigor in Cabbage Seed(Brassica oleracea L. var. capitata)
• Wu Daofan and Song Ming
• Acta Horticulturae Sinica. 2002, 29(6): 542-546.
• Abstract ( 1410 ) HTML ( 1490 ) PDF (135KB) ( 1490 ) 　　　
• Cabbage seeds of different vigor degree were obtained by accelerated aging. Some methods ,Including treatments of imbibition/ dryback , mannitol gibberellin ( GA) , polythyleneglyool ( PEG) , CaCl₂ , KH2PO₄ , ascorbic acid (AsA) , were were investigated to enhance the forgoing seed vigor by L9 (34) orthogonal experiment. Then the changes of cabbage seed at physiological and biochemical level , cell level and structure of tissue cells were investigated. The results indicated that : ①All the 7 methods could improve seed vigor of
  cabbage. Seed vigor was significantly enhanced after seed were treated by all the best effect combination , and PEG, GA treatment was the best one , the difference between PEG, GA and others methods was significant . ②Some physiological and biochemical changes were obvious : protease , dehydrogenase , SOD , CAT and POD were reduced significantly ; relative elelctive conductivity ( REC) and the content of MDA in seed were increased
  significantly. Analysis indicated that they were correlated significantly with vigor , they were the main reason of change of seed vigor , and were reliable indexes of measuring seed vigor (at physiological and biochemical level) .
  ③Karyotype of root tip chromosome showed that the chromosome did not change clearly before and after improving seed vigor. ④Observation on the ultrastructure of the radicle cell of cabbage seed indicated that ultrastructure
  changed with seed vigor reduced , the lower seed vigor , the clearer the change. When seed vigor was improved by all kinds of methods , ultrastructure of cell would be recovered at different degree.
• Related Articles | Metrics

• Effects of Selenium on Antioxidative System and H₂O₂ Content in Spinach
• Li Dengchao;Zhu Zhujun;and Xu Zhihao
• Acta Horticulturae Sinica. 2002, 29(6): 547-550.
• Abstract ( 1486 ) HTML ( 1538 ) PDF (181KB) ( 1538 ) 　　　
• The effects of different concentrations of seleniumon the growth , the activities of superoxide dismutase (SOD) , catalase (CAT) , ascorbate peroxidase (APX) , dehydroascorbate reductase (DHAR) , glutathione
  peroxidase (GSH2PX) and the content of ascorbate (AsA) , hydrogen peroxide (H2O2) were studied in the hydroponic spinach. The results showed that the growth was enhanced by selenium at the low level ( ≤0. 1 mg·L^-1) , but inhibited at the high level (1. 0 mg·L^-1) . The activities of SOD , CAT and GSH2PX were increased and the activities of APX and DHAR were decreased. The contents of AsA and H2O2 decreased with the increasing selenite concentration , whereas , H₂O₂ content decreased firstly and then increased. From our results , we conclude that proper selenite concentration could increase the yield and antioxidative activity of spinach.
  
• Related Articles | Metrics

• Variety Identification and Genetic Diversity Analysis of Rose with RAPDMolecular Markers
• Guo Lihai;Jin Demin;Wang Bin;Liu Min;Chao Yang;Yong Wei;Chen Yuquan;and Weng Manli
• Acta Horticulturae Sinica. 2002, 29(6): 551-555.
• Abstract ( 1260 ) HTML ( 1253 ) PDF (367KB) ( 1253 ) 　　　
• Variety identification and genetic diversity analysis of rose were studied using RAPD technique. A total of 30 individuals were analyzed , 3 specific RAPD markers were found from 65 RAPD bands amplified with 12 primers. One of them was converted to SCAR marker. Phylogenetic relationships of the 30 individuals was developed based on phylogenetic tree conducted with UPGMA method.
• Related Articles | Metrics

• Physiological Bases of Difference Related to Water Deficit Tolerance of Cut Rosecv. Samantha and Gabriella
• Zhang Changqing;Tang Xuemei;Gao Junping;Zhang Hao;and Xiong Li
• Acta Horticulturae Sinica. 2002, 29(6): 556-560.
• Abstract ( 1450 ) HTML ( 1083 ) PDF (176KB) ( 1083 ) 　　　
• In our previous work, 17 commercial cultivars of cut rose (Rosa hybrida) were classified into three levels of tolerance to water deficit by measuring the vase life decrease after 24 h water deficit, at 22- 25 ℃and 40% - 60% relat ive humidity. In this paper, Samantha, a cultivar with strong tolerance to water deficit, and a weak one, Gabriella, were used to determine their vase life, fresh mass loss, water potential of flowers and leaves, leaf stomatal resistance, relative electrolyte conductance, flower ethylene product ion, SOD and POD
  activities of petals and leaves under water deficit. The results showed that as prolonging the t ime of water deficit, the decreased percentage of vase life, fresh mass loss, relative electrolyte conductance and flower ethylene
  production increased, while water potentials of flowers and leaves decreased in both cultivars; andmore remarkable changes were obtained in cv. Gabriella than in Samantha. SOD and POD act ivities of petals and leaves were higher
  in medium range of water deficit than in light and heavy ones. And no remarkable difference of leaf stomatal resistance was found between the two cultivars. Samantha showed a higher abilities to recover from the same water
  deficit than Gabriella during vasing period. Finally the relationships were discussed between the test parameters of petals and leaves and water deficit tolerance of cut roses.
• Related Articles | Metrics

• Technology and Its Mechanism of Color Keeping in Red Rose Petals during theProcessing of Dry Pressed Flowers
• Hong Bo;Liu Xianghuan;and Zhang Xiaofang
• Acta Horticulturae Sinica. 2002, 29(6): 561-565.
• Abstract ( 1670 ) HTML ( 1892 ) PDF (198KB) ( 1892 ) 　　　
• 7 kinds of color protected solut ion for red petals of cut rose ‘Samantha’were made up based on internal and external factors which influence changes of the petal color during pressing and drying of the petals. The
  treatments of the solution were performed with painting of different concentration and with soaking of different time. The results of color protection that very closed to the fresh petals (K₄₀M₁₀₀Y₅₅) were obtained in 9 treatments from 80 treatment combinations, which are A1 0. 5 h soaking, A1-2 painting with 1/ 2 diluted solution, A1-2 painting with 1/ 4 diluted solut ion, A2-6 36 h soaking, A2-6 6 h soaking, A2-6 12 h soaking, A2-9 32 h soaking, A2-9 48 h soaking, and A2-9 56 h soaking. The best result of the color protection was gained in the treatment of A2-9 48 h soaking among the 9 treatments above after 6 months of natural sunshine. The results of pigment analysis showed that there are mainly cyanidin ( 3, 5, 7-trihydro-diphenylpyrane) and 8􀀁hydrolycopene in the petals. The possible mechanism of protect ing permanently the red color after drying of the rose petals is suggested that cyanidin might react with magnesium salt of the color protection solution, producing stable substances such as cyanin and its aglycon.
  
• Related Articles | Metrics

研究报告

• Effect of GA₃ Treatment on Changes of Pigments in Peel of Citrus Fruit
• Tao Jun;Zhang Shanglong;Chen Kunsong;Zhao Zhizhong;and Chen Junwei
• Acta Horticulturae Sinica. 2002, 29(6): 566-568.
• Abstract ( 1272 ) HTML ( 1373 ) PDF (161KB) ( 1373 ) 　　　
• Effects of exogenous GA₃ (100mg/ L) treatment on changes of endogenous hormone , chlorophyll , carotenoid content in peel of citrus fruit were investigated and their possible connections with changes in color and
  lustre of citrus fruit were also examined. The results showed that GA₃ treatment had little effect on ABA content , however , it inhibited decreasing of endogenous GA₃ and ZT content , correspondingly , it delayed chlorophyll degradation and inhibited orange carotenoidβ-cryptoxanthin biosynthesis and acumulation that led to repress development
  color and lustre.
• Related Articles | Metrics

• Photosynthesis of Different Pear (Pyrus L.) Cultivar-rootstock Combinations
• Jiang Weibing;Yu Kaijin;Gao Guanglin;and Ma Kai
• Acta Horticulturae Sinica. 2002, 29(6): 569-570.
• Abstract ( 1443 ) HTML ( 1027 ) PDF (118KB) ( 1027 ) 　　　
• In this paper a comparison of the photosynthetic characteristics among pear rootstock Pyrus betulae folia Bunge and P. calleryana Dcen. , four combinations of two pear varieties Hosui and Niitaka on the above two rootstocks is presented. Results showed that the value of net photosynthetic rate ( Pn) , apparent quantum yield (Φi) , carboxylation efficiency (CE) , and water use efficiency (WUE) of P. calleryana Dcen. , Hosui or Niitaka on P. calleryana Dcen. , Niitaka on either P. calleryana Dcen. or P. betulaefolia Bunge was all higher than that of the above four physiological indices of P. betulaefolia Bunge , Hosui or Niitaka on P. betulaefolia Bunge , Hosui on either P. calleryana Dcen. or P. betulaefolia Bunge respectively.
• Related Articles | Metrics

• Genetic Identity Analysis of Malus xiaoj inensis by AFLPs
• Li Yinghui;Han Zhenhai;Xu Xuefeng;Lu Renqiang;and Qi Liping
• Acta Horticulturae Sinica. 2002, 29(6): 571-572.
• Abstract ( 1458 ) HTML ( 947 ) PDF (108KB) ( 947 ) 　　　
• AFLP was applied in this experiment to detect the genetic ident ity of Malus xiaojinensis from natural population. The fingerprint ing of 20 seedlings of M. xiaojinensis with 8 pairs of primers showed that M. xiaojinensis in natural population had a high degree of genetic ident ity, its genetic similar coefficient being 98. 92 %. Genetic difference also existed in this population, 1. 08 %fragments were different fragments. The patterns analysis
  indicated that these different fragments were resulted from gene recombination of sexual hybridizat ion.
  
• Related Articles | Metrics

• RAPD Analysis for the Identification of the Precocious Trait in Walnuts
• Yang Keqiang;Wang Yuejin;Zhang Yindong;and Zheng Xueqin
• Acta Horticulturae Sinica. 2002, 29(6): 573-574.
• Abstract ( 1253 ) HTML ( 1114 ) PDF (175KB) ( 1114 ) 　　　
• A random amplified polymorphic DNA (RAPD) marker linked to the precocious trait of walnut (Juglans regia L. ) , OPB208900, was identified with bulked segregant analysis ( BSA) . Two bulks of DNA samples were created from open2pollinated Liao 1 (an precocious cultivar) . The bulks were screened using 220 random primers (OP Kit) . OPB208 ( 5. GTCCA CACGG 3. ) generated a specific fragment with precocious individuals and without backward individuals. The specific fragment of approximately 900 base pairs ( bp) , was designated as OPB208₉₀₀. 22 DNAs from 19 cultivars and 3 clones were used as templates for RAPD analysis with primer OPB208 respectively. The specific band of OPB208₉₀₀ was present in 12 out of 17 precocious cultivars and absent in 5 backward cultivars/ clones. The RAPD marker OPB208₉₀₀ is related with precocious trait in walnut and
  would be used to distinguish the precocious trait and backward trait in walnut.
• Related Articles | Metrics

• The Activities of NAD Kinase and NADP Phosphatase and Some Characteristicsof NAD Kinase in Strawberry during Fruit Maturation
• Gu Caiqin;Guan Junfeng;Xi Yufang;and Li Guangmin
• Acta Horticulturae Sinica. 2002, 29(6): 575-576.
• Abstract ( 1586 ) HTML ( 957 ) PDF (94KB) ( 957 ) 　　　
• The act ivities of NAD kinase and NADP phosphatase and some characteristics of NAD kinase in strawberry fruits during maturation were studied. The results showed that from green to white stage, the activities of NAD kinase and NADP phosphatase increased, but decreased from white to pink stage and increased again from pink to red stage in strawberry fruits. The activity of NAD kinasewas inhibited significantly by Ca²⁺ and stimulated by EGTA (a calcium chelator) . The dependence degree of NAD kinase on ATP was increased with the maturation of strawberry fruits.
• Related Articles | Metrics

• Effects of Low Temperature Stress and ABA on Flower Formation and Endogenous Hormone of Litchi
• Zhou Biyan;Li Yubin;Chen Jiezhong;Ji Zuoliang;and Hu Zhiqun
• Acta Horticulturae Sinica. 2002, 29(6): 577-578.
• Abstract ( 1858 ) HTML ( 1238 ) PDF (82KB) ( 1238 ) 　　　
• Litchi trees were treated at low temperature in controlled growing chamber for imitating cold wave. The results showed that abscisic acid (ABA) and indole-3-acetic acid ( IAA) contents of litchi leaves increased , transpiration rate (Tr) decreased and leaf water potential increased during the low temperature treatment . Exogenous ABA treatment raised ABA levels and reduced IAA levels of litchi leaves in the low temperature. Hence the value of
  ABA/ IAA was higher than that of control . Also , Tr decreased and leaf water potential increased. Trees sprayed with ABA remained a relatively high flower formation rate after being treated at low temperature.
• Related Articles | Metrics

• cDNA Library Construction and Identification of Cabbage
• Cao Bihao;Lei Jianjun;Song Hongyuan;Song Ming;Yang Zhaohui;Chengbin Xiang;David. J . Oliver;and Li Danqiong
• Acta Horticulturae Sinica. 2002, 29(6): 579-580.
• Abstract ( 1425 ) HTML ( 1306 ) PDF (92KB) ( 1306 ) 　　　
• A cDNA library of cabbage was constructed and an expression plasmid introduced into E. coli XL12Blue was used as cloning vector. The titer of cDNA library is 2 ×10⁸ - 4 ×10⁸ pfu/ mL ; most of the fragments are more than 500bp , the recombinant rate of the library is about 95 %. Probed with resistant gene fragment , phage in situ hybridization , the hybridization signal is clear , which shows this library is good , and the library had been amplified and stored.
• Related Articles | Metrics

• Head-space Solid Phase Microextraction and GC-MS Analysis of Fragrance ofCucumber
• Liu Chunxiang;He Qiwei;and Liu Yangmin
• Acta Horticulturae Sinica. 2002, 29(6): 581-583.
• Abstract ( 1437 ) HTML ( 2138 ) PDF (145KB) ( 2138 ) 　　　
• The volatile compounds of fresh cucumber were isolated by solid phase microextraction and analyzed with GC-MS 36 kinds of compounds have been found from the two experiments in the GC profile , of them 29
  compounds were identified. The advantage and disadvantage of the two experiments conditions were impersonally evaluated.
• Related Articles | Metrics

• Effect of Illumination and Exogenous Substance on Spore Germination andSeedling Survival of Pteridium aquilinum
• Niu Junyi;Li Sheng;Xu Zuomi;Qin Shuhao;and Lian Rongfang
• Acta Horticulturae Sinica. 2002, 29(6): 584-586.
• Abstract ( 1424 ) HTML ( 1059 ) PDF (147KB) ( 1059 ) 　　　
• This experiment was conducted to study the effect of the different illumination intensities and exogenous substance (GA₃ , 6-BA , humic acid) on pteridium aquilinum’s spore germination and seedling survival . The results showed that the illumination intensity had a direct bearing on the germination percentage and the growing rate of pteridium aquilinum’s spore. There was a remarkable effect on spore germination and seedling
  survival under the treatment of 1 800 lx and continuous lighting , and when illumination intensity was below 1 000 lx , the spores germinated very slowly and could not survive ; the spores couldn’t germinate under darkness.
  There was a significant promotion effect for spore germination , growth and development of survived seedling if the culture was treated by GA₃ , and the survival rate was the highest (61. 42 %) when treated by 0. 2 mg/ L GA₃ .
  The significant mutual effect was not found between illumination intensity and exogenous substance.
  
• Related Articles | Metrics

• Effect of Illumination and Exogenous Substance on Spore Germination andSeedling Survival of Pteridium aquilinum
• Zhai Wenqiang;Tian Qingzhen;Jia Jizeng;and Dong Yuchen
• Acta Horticulturae Sinica. 2002, 29(6): 587-587.
• Abstract ( 1334 ) HTML ( 915 ) PDF (124KB) ( 915 ) 　　　
• Two pairs of MseI/ PstI primer combinations of M21P69 and M17P35 produced polymorphic bands among three Hami Melon hybrids and their parents respectively , which clearly revealed the genetic relationship among hybrid and their parents and can be as the basis of purity identification for Hami Melon hybrid.
• Related Articles | Metrics

• AFLP Analysis of Genetic Relationships of Prunus mume ‘Meiren’and Relatives
• Ming Jun;Zhang Qixiang;Mao Qingshan;and Yan Xiaolan
• Acta Horticulturae Sinica. 2002, 29(6): 588-589.
• Abstract ( 1613 ) HTML ( 1647 ) PDF (132KB) ( 1647 ) 　　　
• The silver-stained AFLP analysis was employed to analyse the DNA fingerprints of four cultivars of Meiren Mei Form , and a wild species , a Pink Double Form and seven relatives. For 420 loci 6 300 bandings was
  capable of producing sharp fingerprint patterns with the primer combinations of E-ACT/M-CAT , E-GA/M-CTC , E-ACT/M-CCA , E-ACT/M-CAC. NTSYSpc 2. 1 was used to SAHN Clustering of UPGMA and according to DICE coefficient , Jaccard coefficient and Simple Matching coefficient (SM) , cluster analysis was done. The relationship among the species was discussed. The result indicated that Prunus mume‘Meiren’was the most close to Prunus
  mume and Prunus cerasifera‘Pissardii’, and distantly related to plum , peach and apricot , conforming to morphological classification and breeding record.
• Related Articles | Metrics

新品种

• A New Pear Variety‘Jimi’
• Wang Yingtao;Li Xiao;Li Yong;and Liu Guosheng
• Acta Horticulturae Sinica. 2002, 29(6): 590-590.
• Abstract ( 1169 ) HTML ( 770 ) PDF (62KB) ( 770 ) 　　　
• The medium pear variety‘Jimi’ is the progeny of hybrid of ‘Xuehua’ and 􀀂Huanghua . The fruit has a oval shape with green to yellow skin and shinning. The average fruit mass is 258 g, the largest fruit mass is 600 g; the soluble solids content is 13. 5 %. The fruit ripen in later August with an ideal product ivity and highly resistant to pear scab.
• Related Articles | Metrics

• ‘Meishuo’—A New Early Ripening, Large Size and Good Quality PeachVariety
• Jia Yunyun;Ma Zhisheng;Chen Tixian;Sun Shufen;Chai Juhua;and Sun Yuzhu
• Acta Horticulturae Sinica. 2002, 29(6): 591-591.
• Abstract ( 2256 ) HTML ( 988 ) PDF (64KB) ( 988 ) 　　　
• ‘Meishuo’ is a new early-ripening peach variety, selected from open-pollinated seedlings of‘Jingyu’. It has some good characteristics, such as early ripening, beaut iful appearance, excellent quality, as well as high yield. It can be planted in adaptable areas of north China.
  
• Related Articles | Metrics

• ‘Fengyue’ and‘Cuiyu’, Two Superior New Kiwifruit Cultivars with GoodStorage Quality
• Zhong Caihong;Wang Zhongyan;and Bu Fanwen
• Acta Horticulturae Sinica. 2002, 29(6): 592-592.
• Abstract ( 1596 ) HTML ( 1000 ) PDF (62KB) ( 1000 ) 　　　
• ‘Fengyue’ and ‘Cuiyu’ are two new cultivars of Actinidia Chinensis , selected from wild kiwifruit. Both cultivars have a average fruit mass of 90 g, with max. 128 g and 129 g respectively. Their fruits are fine and tender in texture with a thick sweet taste and rich refreshing aroma. 􀀂Fengyue fruit have a soluble solids content of 13. 5%- 15. 8% ( max. 19. 0% ) , and the vitamin C content of the fruit is 840- 1 630 mg/ kg pulp, while the data for 􀀂Cuiyu are 14. 5% - 17. 3% ( max. 19. 5%) and 930 - 1 430 mg/ kg pulp,
  respectively. The fruits have good storage quality, especially fruit of‘Cuiyu’ can be stored well for about 30 days at room temperature ( 20 ℃) . 􀀂Fengyue matures in mid to late September in Changsha while 􀀂Cuiyu in early October.
  
• Related Articles | Metrics

• ‘Xinguo Sweet Orange 3-5’A New Sweet Orange Variety
• Zhou Chaohua;Huang Donghua;Xiao Jianxiong;Zhang Jihui;and Zeng Guangjun
• Acta Horticulturae Sinica. 2002, 29(6): 593-593.
• Abstract ( 1330 ) HTML ( 803 ) PDF (63KB) ( 803 ) 　　　
• ‘Xingguo sweet orange 3-5’ is a new variety selected from the escendants of ‘Xingguo sweet orange’. Fruit is from orange􀀁yellow to salmon pink and nearly globe. The average fruit mass is 167. 7 g. The soluble solid content is 13. 6% - 15. 1% . The total sugar and acid contents are 10. 3% - 11. 5% and 0. 47%-0. 93% , respectively. The fruit has a edible part of 79. 2% - 80. 75%, and juice rate is 64. 18% - 65. 96%.
  
• Related Articles | Metrics

• ‘Jifeng’— An Excellent Late Fruiting New Walnut Variety
• Bai Zhongkui and Zu Wenfang
• Acta Horticulturae Sinica. 2002, 29(6): 594-594.
• Abstract ( 1468 ) HTML ( 788 ) PDF (61KB) ( 788 ) 　　　
• ‘Jifeng’ is a late fruiting walnut variety, ehibiting excellent characterist ics of moderate-size fruit, thin shell, plump kernel, high yield and good quality. The average fruit mass is 11. 19 g, the thickness of shell is 1. 14mm, the ratio of kernel is 58. 5 %, and the content of coarse fat is 68. 53% . It grows vigorously with high adaptability to adverse growing conditions. It is suitable to be planted inHuabei walnut growing area and other place with the same soil and climate condit ions.
• Related Articles | Metrics

• A New Variety for Both Fresh and Processing Use —‘Shunhua Blueberry 3’
• Zhang Qichang;Qi Qige;Wu Banghua;Lin Baoshan;Qu Huanyun;Huang Zhiping;Sun Xuelun;and Jiang Weiqing
• Acta Horticulturae Sinica. 2002, 29(6): 595-595.
• Abstract ( 1481 ) HTML ( 1049 ) PDF (88KB) ( 1049 ) 　　　
• ‘Shunhua Blueberry 3’is a good variety for both fresh and processing use with the characteristics of middle maturity , cold resistance , high and stable yield , unique taste. In eastern mountain area in Jilin province its yield is 9 521 kg/ hm² , 15 % higher than that of Blomidon when it is three years old. Itsyield is 9 850 kg/ hm² , 17 % higher than that of Bolmidon when it is four years old.
  
• Related Articles | Metrics

• A New Line Pepper Cultivar‘Shanjiao 2001’
• Zhao Zunlian and Shi Lianlian
• Acta Horticulturae Sinica. 2002, 29(6): 596-596.
• Abstract ( 1303 ) HTML ( 974 ) PDF (68KB) ( 974 ) 　　　
• ‘Shanjiao 2001’ is a line pepper cultivar that is suitable to cultivate in Shaanxi and other similar pepper production areas. This cultivar is resistant and/ or tolerant to CMV, BBWV etc. At the same time it has some resistance to anthracnose and Phytophthora blight. The length and width of fruit are 13. 4 cm and 1. 28 cm respectively. The ripe fruit is red. The contents of vitamin C, vitamin B2 and crude fat are 1. 804 mg/ g, 2. 90 µg/ g and 1. 56%, respectiverly. The yield is increased by 25. 76% over the control.
• Related Articles | Metrics

• A New Giftwarely Watermelon Cultivar‘Xiangyu Bingjing’
• Tao Dihui;Liu Jianxiong;Ruan Wanhui;Chen Rong;and Xiao Jun
• Acta Horticulturae Sinica. 2002, 29(6): 597-597.
• Abstract ( 1394 ) HTML ( 754 ) PDF (64KB) ( 754 ) 　　　
• ‘Xiangyu Bingjing’watermelon is a hybrid. Its female parent is selected by the pedigreed method from ‘Taiwan Texiaofeng’and the male parent is introduced from Xinjiang. It is characterized by crispy with good quality, early maturity, tolerance to low temperature, 1- 1. 5 kg of per fruit mass, round fruit with strip rind, thin skin, and higher marketable yield.
  
• Related Articles | Metrics

• A High Quality and Early Maturity New Hybrid Watermelon‘Tianci 608’
• Mao Weihai;Zhang Weizhang;Bao Chonglai;Sun Lixia;Hu Tianhua;and Hu Qizan
• Acta Horticulturae Sinica. 2002, 29(6): 598-598.
• Abstract ( 1351 ) HTML ( 786 ) PDF (63KB) ( 786 ) 　　　
• ‘Tianci 608’ is a high quality and early maturity watermelon hybrid. It can be harvested in 28-29 days after pollinating, and has a very good fruit-setting prorerty, medium resistance to fusarium wilt . The fruit is eliptic in shape with red flesh, its central part sugar content is 12%. The average single fruit mass is 3. 5-4 kg. The variety is suitable for early culture in protected or open fields in main watermelon growing region.
• Related Articles | Metrics