研究论文

• The Kinetics of Calcium Uptake in Apple Rootstock Roots
• Yang Hongqiang;Zhang Lianzhong;Qi Jinliang;Jie Yuling
• Acta Horticulturae Sinica. 2003, 30(03): 253-257.
• Abstract ( 1805 ) HTML ( 1905 ) PDF (219KB) ( 1905 ) 　　　
• The experiment were carried using apple seedlings(Malus hupenensis Rehder)and pot—cultured three—year old apple tree(Fuji/Malus hupenensis Rehder)．It was studied that the kinetics of Ca²⁺ uptake and the effect of temperature and metabolic inhibitor 2，4-Dinitrophend(2，4-DNP)on Ca²⁺ uptake rate. The result show that the relation of Ca²⁺ uptake rate and Ca²⁺ concentration fits into Michaelis-Menten model under low concentlation of Ca²⁺ (0.02—0.5 mmol·L^-1)．The Ca²⁺ uptake rate rise linearlv with the increase of Ca²⁺ concentration in both intact roots and excised roots and the rate of Ca²⁺ uptake in intact roots exceeds in excised roots under high concentration of Ca²⁺ (0.5-5.0 mmol·L^-1)．The rates of Ca²⁺ uptake were inhibited by 2，4-DNP both in intact roots and excised roots．The inhibition rate was bigger under low concentration than high concentration and in excised roots than in intact roots. Under low concentration,the rate of Ca²⁺ uptake Was influenced markedly by temperature．The α value and Ca²⁺ uptake rate in excised roots were the highest under 15—20 ℃．Under high concentration,the rate of Ca²⁺uptake did not afected markedly by temperature．The Ca²⁺ uptake rate in absorbing roots was similar to extensive roots according to roots surface area．The α value in
  ligneous roots was 54% to absorbing roots and extensive roots．
  
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• Efects of Rootstocks on Photosynthetic Properties of Young ‘Hosui’ PearTrees under Salinity
• Gao Guanglin;Jiang Weibing;Wang Liangju;Han Haozhang;Dai Meisong
• Acta Horticulturae Sinica. 2003, 30(03): 258-262.
• Abstract ( 1576 ) HTML ( 1175 ) PDF (205KB) ( 1175 ) 　　　
• The effect of two pear rotstocks ‘Calleryana’ and ‘Betulaefolia’ on photosynthetic properties of young ‘Hosui’ under salinity was measured by portable photosynthesis systems CIRAS-1．The results showed that：(1)There are obvious discrepancy between Pyrus betulaefolia and P.calleryana efect on the photosynthetic properties of Hosui blade; (2)Pn，Gs，Tr，and CE were higher in Hosui combined with P.betulaefolia (H-B)than in Hosui comb ined with P.calleryana (H-C)under the same conditions，H-C with higher Ci than H-B under high salinity．(3)Photosynthesis of pear blade was inhibited by salinity most cause for stomatal limitation under low salinity at early time，and most cause for nonstomatal limitation under high salinity and low salinity at anaphase；(4)In anaphase oftreatment，the discrepancy between P．betulaefolia and P.calleryana efect on photosynthetic properties was dimi nished under severe salinity．
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• The Relationship between Ca²⁺-CaM and Ethylene Autocatalysis in Strawberryand Tomato Fruit
• Gu Caiqin;Xi Yufang;Guan Junfeng;Li Guangmin
• Acta Horticulturae Sinica. 2003, 30(03): 263-266.
• Abstract ( 1730 ) HTML ( 1507 ) PDF (201KB) ( 1507 ) 　　　
• Strawberry and tomato fruits harvested at white and green mature stages respectively were investigated in relation to ethylene production rates and calmodulin (CaM) contents．The fruits were treated for 12 h with exogenous ethylene in combination with verapamil (Vp) or chloropmmaize (CPZ) and trifluoperazine (TFP)，and then removed from the ethylene for strawberry．The results showed that exogenous ethylene could increase ethylene production of strawberry by420%-73% and resulted in a peak of ethylene production of toma to fruit with 2 days in advance. Ethylene also increased the CaM contents in both fruits. Vp,CPZ and TFP restrained the ethylene production in strawberry fruit when ethylene applied to the fruit，which indicated that Ca²⁺-CaM signal

  system maybe involved in ethylene autocatalysis. Vp,but CPZ and TFP， can inhibited the ethylene autocatalysis of tomato fruit，which suggesting that，in contrast to strawberry，ethylene autoeatalysis of the fruit was related to

  Ca²⁺ influx from the extracellular space， but to CaM ．
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• Cloning and Expression Analysis of LEAFY Homologues Genes from Apple
• Cao Qiufen;Wada Masato;Meng Yuping;Huang Jing;Sun Yi;Wang Guoping
• Acta Horticulturae Sinica. 2003, 30(03): 267-271.
• Abstract ( 1621 ) HTML ( 1479 ) PDF (241KB) ( 1479 ) 　　　
• Two LEAFY like cDNA fragments of in length，AFL1 and AFL2 were cloned from the terminal buds of bearing shoot of apple trees．Sequencing analysis showed that the coding regions of AFL1 and AFL2 had 90% homolegy，but the 3’non-coding regions had less than 60% homology．The deduced amino acid sequence of AFL1 or AFL2 cDNA had over 70% similarity with PTFL ， PEAFLO and ALF genes of poplar、pea and petunia， and less than 70% similarity with TOFL， FLO and LFY genes of tomato， antirrhinum majus and arabidopsis respectivehy. RT-PCR analysis showed that AFL1 expressed only in the telminal buds during the transition period from vegetative to reproductive growth; whereas AFL2 expressed in the sepal, ovary, root, stem, leaf and the terminal buds between June to Oct. These results might suggest that AFL1 and AFL2 had a common origin， and their functions had diverged， so that they appeared to play independent roles inⅡ0Ial and vegetative tissue development．Southem blot analysis showed that there were at least two copies of LEAFY homo logues genes in Malus species and pear.
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• Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP

  Analysis
• Yi Ganjun;Tan Weiping;Huo Heqiang;Zhang Qiuming;Li Jianguang;Zhou Birong
• Acta Horticulturae Sinica. 2003, 30(03): 272-276.
• Abstract ( 1506 ) HTML ( 2139 ) PDF (263KB) ( 2139 ) 　　　
• The study is mainly on the genetic diversity and relationship of Longan (Dimocarpus longan Lour．) cultivars by applying AFLP (Amplified Fragment Length Polymorphism). 103 polymorphic distinct bands were obtained with two pairs of primers(EcoRⅠACT+Mse ⅠCTT and EcoR ⅠACT+Mse Ⅰ CTC)，which were enough to distinguish the 46 cultivars from each other. UPGMA analysis showed that 46 cultivars were divided into 11 groups．The ratio of polymorphic rate varied from 0.2703 to 0.4955．The result showed that the Fujian Dongbi

  was diferent from Guangdong Dongbi and they were different types from Kaiyuansi Dongbi．Zaoshu 1 was supposed to be a new type．The results supplied theoretical basis for cultivar classification．
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• Regulation of 1-methylcyclopropene on the Ripening and Softening of Postharvest Kiwifruit
• Ding Jianguo;Chen Kunsong;Xu Wenping;Xu Changjie
• Acta Horticulturae Sinica. 2003, 30(03): 277-280.
• Abstract ( 1602 ) HTML ( 1704 ) PDF (167KB) ( 1704 ) 　　　
• The fruit of Kiwifmit (Actinnida deliciosa ‘Bruno’) was used to investigate the regulation of 1·MCP on fruit ripening and softening at 20 ℃． The results showed that there were two distinct stages of fruit softening， i.e. the initiating stage of softening or the stage of slowly softening, and the stage of fast softening in ripening fruit and that the process of system Ⅰ transforming to system Ⅱ ethylene biosynthesis and the ethylene climacteric peak were postponed in the fruit treated with 1·MCP，which result a slowed softening rate being slowed and influence the activities of LOX and AOS, and the up·stream regulators of ethylene biosyn thesis，
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• The Different Changes of Endogenous Polyamines in Tomato Plants th DifferentHeat-tolerance under High Temperatures
• Du Yongchen;Mao Shengli;Wang Xiaoxuan;Zhu Dewei;Li Shude;Dai Shanshu;Gao Zhenhua
• Acta Horticulturae Sinica. 2003, 30(03): 281-286.
• Abstract ( 1581 ) HTML ( 1272 ) PDF (269KB) ( 1272 ) 　　　
• The changes of endogenous polyamine levels induced by high temperatures were investigated in tomato plants with diferent heat-tolerance. Plants were treated at temperatures of 25／20oC (day and night)，33/25 ℃ and 38/28℃ at seedling or blooming stage. The results showed that the levels of spe rmidine and spermine in heat-tolerant varieties significantly increased with increase of growing tempe rature． and the maximum was observed at 38/28℃．In contrast， in heat-sensitive varieties，the highest levels of spermine and spermidine occurred at 33/25℃. The temperature treatment of 38/28℃ remarkedly decreased spermine and spermidine contents both in leaves
  and flowers compared with 33/25℃ in heat．sensitive varieties．The putrscine content showed no regular changes both in temperature treatments and varieties. The involvement of polyamines in tomato heat tolerance is discussed.
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研究简报

• Effects of Cold Storage and Controlled Atmospheres on the Content of Giucoraphanin in Broccoli Florets
• Wang Qiaomei;Hu Jiashuz
• Acta Horticulturae Sinica. 2003, 30(03): 286-286.
• Abstract ( 1460 ) HTML ( 817 ) PDF (56KB) ( 817 ) 　　　
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研究论文

• Studies on Germination Character of Wasabi (Wasabi japonica) Seeds
• Wu Zhen;Wang Guangdong;Weng Mangling;Li Shijun
• Acta Horticulturae Sinica. 2003, 30(03): 287-290.
• Abstract ( 2046 ) HTML ( 2891 ) PDF (171KB) ( 2891 ) 　　　
• The germination character of wasabi seeds an d efect of diferent temperature, light and plant hormones, chemicals on germination percentage of wasabi seeds were studied． The results showed that germination percentage was the highest under 15 ℃，and germination percentage under 20 ℃ was lower than that under 15℃. 6-BA，GA₃，and BR could increase germination percentage of wasabi seeds among all treatments，6-BA acted the most efficiently．When seed coat was removed，germination percentage might arrive 98.0%,and germination speed became quickly，so seed coat could inhibit germination of wasabi seeds.
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研究简报

• Heavy Metals Contents in Vegetables in Guangzhou Clean Vegetable Garden Soils
• He Jianghua;Liu Yong;Wang Shaoyi;Chen Junjian;;Wei Xiuguo;
• Acta Horticulturae Sinica. 2003, 30(03): 290-290.
• Abstract ( 1396 ) HTML ( 1609 ) PDF (171KB) ( 1609 ) 　　　
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研究论文

• The Effects of Inhibiting Lipoxygenase on Microtuber Formation and Growth ofSolanum tuberosum L. in Vitro
• Ma Chongjian;Liu Jun;Xie Conghua
• Acta Horticulturae Sinica. 2003, 30(03): 291-295.
• Abstract ( 1533 ) HTML ( 1227 ) PDF (242KB) ( 1227 ) 　　　
• The single node segments of planflets of potato cultivars ‘Mim’ and ‘E-potato-1’ were cultured with salicylhydroxamic acid，the inhibitor of LOX，to identify the effects of LOX on tuberizafion in vitro. The resuits showed that activity of LOX in both the planflets and tubers as well as the concentration of jasmonic acid (JA)
  were decreased markedly in the treatments with inhibitor, accompanying by the declines of percentage of tuber formation by planflets, the number of tubers per plantlet and nlean tuber nlass．The activity of LOX in stolons was the highest. It indicated that LOX plays important roles in tuberization and tuber bulking of potatoes, possiblely through the regulation of JA biosynthesis．
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• Regulations of Fiber Synthesis in Excised Pea Seedlings by Gibbereilic Acid, Ethylene and Abscisic Acid
• Jiang Weibo;Liu Zunying;Feng Shuangqing;Zhao Yumei
• Acta Horticulturae Sinica. 2003, 30(03): 296-298.
• Abstract ( 1498 ) HTML ( 1267 ) PDF (145KB) ( 1267 ) 　　　
• To learn how the fiber-synthesis re,dated by plant growth resistors,excised pea seedlings (Pisum sativum L．) were treated with gibberellic acid(GA)，ethylene or abscisic acid(ABA)．rI1le fiber level in the seedlings uauy increased after harvest and it was further enhanced by ethylene or ABA，and reduced by
  GA，whereas，activities of related emymes(phenylalanine deaminase, polyphenol oxidase，cinnamyl alcohol dehydmgenase and pemxidase) in the seedlings were enhanced by treatment with ethylene or ABA，However cinnamyl alcohol dehydmgenase activity was significantly reduced by GA．
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• Phylogenetic Relationship among Several Subgenus Yulania Species Based onRAPD Markers
• Wang Yaling;Li Yong;Zhang Shouzhou;Cui Tiecheng
• Acta Horticulturae Sinica. 2003, 30(03): 299-302.
• Abstract ( 1579 ) HTML ( 1315 ) PDF (168KB) ( 1315 ) 　　　
• In this study, 20 materials of subgenus Yulania (include species and cultivars)were analyzed using random amplified polymorphic DNA markers. 15 primers were screened from 55 arbitrary 10-mer primers, and a total of 274 DNA bands were amplified，262 of which (95.6%)were polymorphism．According to the data, a tree diagram of phylogenetic relationship was constructed using UPGMA in MEGA．The results showed that the gene background of sub genus Yulania is very complex and it was difficult to be classified. So know that the resource of subgenus Yulania in China were abundant．
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• Responses of Proline Content and Activity of Antioxidant Enzymes in Warmseason Turfgrasses to Soil Drought Stress
• Lu Shaoyun;Chen Siping;Chen Siman;Liang Xiao;Guo Zhenfei
• Acta Horticulturae Sinica. 2003, 30(03): 303-306.
• Abstract ( 1803 ) HTML ( 2088 ) PDF (189KB) ( 2088 ) 　　　
• Relative water content and relative electrolyte leakage of three warm-season turfgrasses, Tifdwarf bermudagrass(Cynodon dactylon×C.transvaalensis), manilagrass [Zoysia matrella (L．)Merr.]and carpetgrass [Axonopus compressus (Sw.)Beauv.] were measured after they were subjected to soil drought stress
  The data showed that Tifdwarf and manilagrass had high levels of relative water content (RWC) and low levels of relative electrolyte leakage(REL), whereas carpetgrass showed rapid decline of RWC and rise of REL under soil
  drought stress. The results indicated that the drought tolerance decreased in following order: Tifdwarf > manilagrass>earpetgrass. Proline was accumulated in above three species with a negative correlation to drought stress， indicating that proline accumulation in turfgrasses was resulted from the injury by drought stress. Carpetgrass showed continuous increase in superoxide dismutase (SOD) activity，a transient rise of catalase(CAT)activity and reduction of peroxidase (POD) activity，which may lead to accumulation of H₂0₂ and therefore injury to plants. Tifdwarf and manilagrass had a transient rise of SOD activity，and prolonged increase in aetivities of CAT and POD. The results indicated that antioxidant enzymes were associated to drought tolerance of warm-season turfgrasses.
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研究报告

• A Revised Division of the Developmental Stages in Litehi Fruit
• Li Jianguo;Huang Huibai;Huang Xuming
• Acta Horticulturae Sinica. 2003, 30(03): 307-310.
• Abstract ( 1859 ) HTML ( 1430 ) PDF (160KB) ( 1430 ) 　　　
• It was suggested that litchi fruit growth could be reasonably divided into two stages(Stage I andStage II)，and stageⅡ could be further subdivided into two substages(Stage II a and Stage II b)．Stage I，which constitutes about two thirds of the whole fruit growth cycle(0-53 DAA)，was the phase mainly characterized by the growth of pericarp and seedcoat，during which the influx of solutes was slower than the influx of water into the seed，and it Was almost the same case for the pericarp．Stage II a was the phase mainly characterized by the growth of embryo(53—67 DAA)and the influx of solutes Was faster than water influx into the fruit during this stage．Stage II a was not distinct in fruit with aborted seed．Stage II b(67—88 DAA)was the phase characterized by rapid aril growth and maturation，when the infl ux of solutes Was slower than water infl ux into the aril and the ma turation progressed rapidly．Clustering analysis results of changes in fruit dry mass confi rmed that this revised
  division of two developmental stages Was in accordance with the ma thema tical pattern and wi th the notion of“Ballskin versus bladder efect”proposed by Huang and Xu(1983)．
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• Efects of Ectomycorrhizal Fungi on the Growth and Nutrient Uptake of Chestnut(Castanea mollissima BL．)
• Feng Gu;Xu Bing;Qin Li;Li Xiaolin
• Acta Horticulturae Sinica. 2003, 30(03): 311-313.
• Abstract ( 1810 ) HTML ( 1547 ) PDF (132KB) ( 1547 ) 　　　
• In order to understand the the effects of ectomycorrhizal fungi on the growth and phosphorus nutrition status of chestnut seedlings，experiment using three-compartment mesh boxes were carried out under controlled conditions．Two chestnut seedlings were transplan ted in the central compartment and inoculated with two ectomycorrhizal fungi Boletus edulis or Suillus luteus or not inoculated. The result showed that the contribution of extmradical mycellium to the total uptake of P in plant were 39％-54％ in Boletus edulis and 15％-35％ in Suillus luteus . Comparing with noninoculated treatment，inoculating with both fungi decreased Olsen-P and pH of soil in hyphae compartment．which indicates that both fungi aIe able to mobilize phosphates that are seareely-soluble and low availability in the soil．It is concluded that both ectomycorrhizal fungi play crucial role for P nutrition and the growth of chestnut．
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• Studies on Photosynthetic Characteristics of Rubus strigosus Mick.
• Li Liulin;Yang Peifang;Tian Caifang;Cui Ruguang;Gao Wanmin
• Acta Horticulturae Sinica. 2003, 30(03): 314-316.
• Abstract ( 1479 ) HTML ( 1560 ) PDF (129KB) ( 1560 ) 　　　
• Photosynthetic characteristics were carried out with Rubus strigosus Mick．The results indicated:the curve of diumal variation of Rubus strigosus Mick. leaf photosynthesis showed two peaks，appeared ‘Wuxiu’ phenomenon．Pn was positively significantly correlated with Gs，at the same time，the variation of Pn，Gs and Ci was synchronism； the principal environmental factors，which efected Pn，were PFD and RH；the curve of seasonal variation had two peaks; The light compensation point of photosynthesis and light saturation point of Rubus strigosus Mick．1eaves were 750 μmol·m^-2·S^-1and 21.4 μmol·m^-2·S^-1, respectively．
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• Studies on Shoot-tip Culture of Prunu pseudocerasus
• Chen Junzhi;Li Qing;Sun Zhao
• Acta Horticulturae Sinica. 2003, 30(03): 317-318.
• Abstract ( 1602 ) HTML ( 1108 ) PDF (84KB) ( 1108 ) 　　　
• Using the shoot—tips as the explant，the tube propagation of Prunus pseudocerasus was studied. The optimal asepsis and culture condition were established by a statistical method. The explant was asepticized as follows: 70％ ethanol 30 seconds→ 0.1％ benzalkonium bromide solution 15 minutes→0
  .1％ HgCl₂ 3 minutes, besides 50 mg/L carbenicillin must be added to the media．The optimal culture condition as follows：(1)the medium for primary propagation contained F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L，； (2)the medium for multiple propagation contained F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L， 2％sugar and 0.5％ sorbitol．The results also showed that diferent explants． the length of shoot-tip and the application of anti-oxidant could afect the surviving rate．
  
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• A Preliminary Study to Evaluate the Antioxidative Capacity of Banana by Comparing the Diameter of Methyl Viologen Speckles
• Ke Desen;Xu Zhifang;Sun Guchou
• Acta Horticulturae Sinica. 2003, 30(03): 319-321.
• Abstract ( 1551 ) HTML ( 1223 ) PDF (141KB) ( 1223 ) 　　　
• After 10 h illumination under the light of 50 μmol·m^-2 ·s^-1，obvious speckles could be formed in the place where the Methyl Viologen was dripped as little blobs on the surface of banana leaves. By determining the activity of two chief antioxidative enzymes，superoxide dismutase(SOD)and catalase(CAT)，a significant reverse linear relationship was found between the diameter of Methyl Viologen speckle and the two enzymes activities, showing the close relationship between the speckle diam eter and the antioxidative capacity in bananas． Exogenous active oxygen species could induce the damage such as peroxidafion of membrane lipid in banana leaves , the damaging degree is closely related with the diam eter of Methyl Viologen speckles，this result indicated that the rapid method to evaluate an tioxidation capacity in bananas was feasible.
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• LEA₃ Gene Transferred into Strawberry
• Wang Junli;Ge Huibo;Peng Shiqi;Zhang Hongmei;Xu Jiuru
• Acta Horticulturae Sinica. 2003, 30(03): 322-324.
• Abstract ( 1409 ) HTML ( 1331 ) PDF (130KB) ( 1331 ) 　　　
• Callus of anther from strawberry (Fragaria ananassa Duch. Darslect) was induced on MS medium. Late embryogenesis abundant protein gene，LEA3，from barley(Hordeum vulgare L．)was introduced into strawberry callus by the aid of biolistic-mediated transformation method．After 4 times of PPI’selected culture. resistant calli and regenerated plants were obtained．The hybridization bands were determined in diferent transgenic plants by Southem blot，and this showed that LEA₃ gene was integrated into strawberry genome.
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• Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine
• Gu Xiaofeng;Luo Zhengrong
• Acta Horticulturae Sinica. 2003, 30(03): 325-327.
• Abstract ( 1563 ) HTML ( 1252 ) PDF (153KB) ( 1252 ) 　　　
• A study of inducing polyploidy by treating ‘Luotian Tianshi’persimmon in vitro leaflet explants with colchicine solution was perfonned．The results indieated that mutated planflets can be induced by treating the leaflet in 0.3％ colchicines solution for 4—6 d．Stomata of the mutated planflets were significantly larger than those of the normal planflets．but the density of stomata were reduced signmcantly．The chromosome number of root—tip cell of mutated plantlets was 2n=12x=180， and nuclear DNA content of the mutated planflets were twice as much as normal hexaploid plantlets．These results suggest that the ploidy of mutated planflets was autododecaploid．
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• Observation and Comparison on Pollen Morphology of Different Ploidy Watermelon
• Liu Wen’ge;Wang Ming;Yan Zhihong
• Acta Horticulturae Sinica. 2003, 30(03): 328-330.
• Abstract ( 1733 ) HTML ( 1698 ) PDF (150KB) ( 1698 ) 　　　
• Morphological characters of the pollen grain of diploid, triploid and tetraploid watermelons (Citrullus lanatus)were observed with the aid of scanning electron microscope．Population polen of the diploid waterm elon were in good order； their pollen’s shape with 3 apertures was subprolate and average P/E ratio was 1.283；The polen grains of the tetraploid was spheroidal with 3,4 and more ape rtures， average pollen’s P／E ratios was 1.111; Heteromophic, adhesive and shrivelled pollen were observed on tetraploid watermelon．All of the pollen grains of the triploid were shrivelled . The reasons of lower germination of antotetraploid pollen and abotive pollen of triploid were discussed from palynological observations．
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• The Major Characters Apperance of Parents in the Triple Hybrid in Muskmelon
• Li Xiuxiu;Lü Jinggang;Xue Yiliu
• Acta Horticulturae Sinica. 2003, 30(03): 331-334.
• Abstract ( 1474 ) HTML ( 1005 ) PDF (140KB) ( 1005 ) 　　　
• Four strains used as female parents were crossed with 8 selflines separately, 26 triple hybrids were obtained. The variation degree of main characters, diferent significan ce of triple hybrids, the relevance of fruit shape betwen the triple hybrids and its parents’ were all analysed．The results show:the variation degree of fruit mass of triple hybrids Was the biggest(variation coefficient was 15-28)；then was the sugar (5-16)；the fruit shape was relatively stable (<10)，the diference of its variation degree and the double hybrids was not
  significant，the fruit shape of the triple hybrids was relevan t with its male parent； the variation degre of blossom stage in the same triple hybrid Was very small.
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• Studies on Postlmrvest Senescence Physiology of Brassica campestris L. ssp. chinensis (L.) Makino.
• Hou Jianshe;Xi Yufang;Yu Ting
• Acta Horticulturae Sinica. 2003, 30(03): 335-337.
• Abstract ( 1630 ) HTML ( 1340 ) PDF (124KB) ( 1340 ) 　　　
• The ethylene production of ‘Zaoyoudong’ Chinese cabbage [Brassica campestris L．ssp． chinensis (L．) Makino. var. communis] tended to decline, the weight-loss increased rapidly，and respiration rate showed a climacteric-like pattern with climacteric on 6th day in the duration of storage under the conditions of (20±1) ℃。85％-95％ relative humidity．The content of chlorophyll，soluble proteins and carotenoid in outer lcaves decreased more rapidly than that in inner lcaves during storage．In outer leaves there were marked chan ges in

  supemxide dismutase(SOD)and catalase(CAT)activities and the activities of the two enzymes decreased rapidly after 4 days of storage with a mole obvious decrease in CAT activity．Pemxidase(PoD)activity and malondialdehyde(MDA)content increased gradually and then rose sharply after 6 days and 4 days of storage， respectively，indicating an inbalanced metabolism of activated oxygen occurred and resultant accumulation in activated oxygen was

  associated with membrane lipid pemxidation．In inner lcaves the activities of both SOD and CAT were at high level and varied very little while POD activity didn’t increased significantly。but MDA concentration declined a little. These data indicated a balance between the production and elimination of activated oxygen existed in iBrier lcaves prevented the accumulation in activated oxygen and the progress of me mb rane lipid pemxidation. It could be concluded that the senescence of B.chinensis var．commumis was caused by maladjusted metabolism of activated oxygen with outer leaves being senesced much easier than inner leaves， therefore， the key factor for storage of Chinese cabbage is to delay the senescence in outer leaves.
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• Influence of Diferent Harvest Dates and High-drying Temperatures on the SeedQuality of‘ 'Jin Ganlan 4' Cabbage
• Zhang Guangxing;Chen Xinjuan
• Acta Horticulturae Sinica. 2003, 30(03): 338-340.
• Abstract ( 1484 ) HTML ( 1162 ) PDF (133KB) ( 1162 ) 　　　
• When cabbage was harvested 45-55 days post anthesis， the germination percentages of all trealments could be larger than 98％. The thousand seed mass, germinating vigor， POD activity and TTC value increased considerably with the delay of harvest dates, but EC value decreased considerably. When the seed lots of cabbage were chied at 40-60℃, there were no remarkable influences on the thousand seed mass and germination percentage， but there was remarkable decreases in the germinating vigor, POD activity and TTC value when dried at 50℃ and 60℃.
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• Expression and Inheritance of Snowdrop Lectin Gene (gna) in Chinese Cabbage
• Yang Guangdong;Zhu Zhen;Li Yan’e;Zhu Zhujun;Shangguan Xiaoxia;Wu Xia
• Acta Horticulturae Sinica. 2003, 30(03): 341-342.
• Abstract ( 1486 ) HTML ( 1169 ) PDF (136KB) ( 1169 ) 　　　
• Molecular test and insect bioassays with aphid (Myzus persicae Sulzer) larvae verified that gna gene had been integrated into Chinese cabbage genome mediated by Agrobacterium tumefaciem ，and all 7 transgenic plants were aphid resistant，strong mRNA of gna could be detected in transgenic plants with higher insect resistance. The results of inheritance analysis showed foreign gene in 2 transgenic Chinese cabbage plants 282-3 and 282-9 is in the model of the simple Mendel’s fashion in their T₁ progenies.
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• A High Efficient Plant Regeneration System from Callus of Pteridium apuilinum
• Jiang Changyang;Ning Shuxiang;Yu Miao;Wang Yu;Song Lixiu
• Acta Horticulturae Sinica. 2003, 30(03): 343-345.
• Abstract ( 1323 ) HTML ( 1056 ) PDF (149KB) ( 1056 ) 　　　
• Induction, diferentiation and rooting of different explants of Pteridium apuilimun were described on different medium in which contained different hormones and a high egicient plant regeneration system was established. The result showed that the most suitable medium was 1/2 MS+0.2 mg/L+IBA 0.4 mg/L+NH₄H₂PO₄; the induced frequency of bud from callus was 100％ on medium in contained 1/2 MS； The radiation rate of adventitious seedling was 100％ on medium 1/2MS + 0.2 mg/L IAA, simultaneously vigorous rhizome was formed and the survival rate of transplantation of test-tube seedling could reach a high level at 94％.
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• Cloning of a cDNA Fragment of Acid lnvertase Gene from Cucumis melo L．Fruit
• Yu Xiyan;Zhao Shuangyi;He Qiwei;Kong Qingguo
• Acta Horticulturae Sinica. 2003, 30(03): 346-348.
• Abstract ( 1524 ) HTML ( 1243 ) PDF (149KB) ( 1243 ) 　　　
• PCR primers were designed based on the eonsensu$ domain of some acid invertase genes in GenBank．The aimed cDNA fragment was amplified from the total RNA isolated from melon fruit via RT-PCR and cloned into pMD18-T vector. The amino acid sequence determined by this fragment shows 99％ homology to that of the corresponding region of acid invertase of tomato. This confirms that we have cloned a cDNA fragment from melon fruit successfully. the access number of this gene in GenBank is AF490425.
  
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• Researches on Flower Bud Morphodiferentiation of Daphne odora var. narginata
• Huang Donghua;Zhou Chaohua;Wei Guowen;Song Xiaomin;Hu Xinlong;Xiong Detao;Chen Deying
• Acta Horticulturae Sinica. 2003, 30(03): 349-351.
• Abstract ( 1616 ) HTML ( 1191 ) PDF (161KB) ( 1191 ) 　　　
• Flower bud of Daphne odora var. narginata Mak. turns from terminal bud. Its diferentiation includes two processes of diferentiation of inflorescence and of floret, can be divided into seven developmental stages：undiferentiation, different beginning, differentiation of inflorescence, floret, petal, stamen and pistil. The flower bud differentiation begins on May middle and requires 6—7 months. Flower bud differentiation is individual and may be dirided two rapid times. The bud diferentiafton is affected by temperature et al.
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研究简报

• Effect of Crop Load on Photosynthetic Rate and Dry Matter Production of Apple
• Ran Xintuo;Zhang Xinsheng;Wang Xuejun
• Acta Horticulturae Sinica. 2003, 30(03): 351-351.
• Abstract ( 1336 ) HTML ( 734 ) PDF (44KB) ( 734 ) 　　　
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研究报告

• Efect of Water Deficit Stress on Endopeptidase Activity in Petals of Cut Rose‘Belami’
• Cong Richen;Zhao Xiting;Gao Junping
• Acta Horticulturae Sinica. 2003, 30(03): 352-354.
• Abstract ( 1457 ) HTML ( 1718 ) PDF (135KB) ( 1718 ) 　　　
• Flowers with branches of cut rose ‘Belami’ (Rosa hybrida ‘Belami’)，a cultivar with middle range tolerance to water deficit stress, were treated with 30 h water deficit stress at room temperature and 40％relative humidity. Then the flowers were reput in water, and vased continuously until loss of vasing value．
  Changes of the contents of soluble protein and free amino acid， and endopeptidase activity in petals were determined. The contents of soluble protein and free amino acid were increased obviously in treated petals as
  como．-,~1 with untreated contro1． and the efect Was lasted even after rehydration and during flowers vasing．
  Endopeptidase activity of petals in treated flowers Was much higher than in control immediately after treatment，
  however the activity in treated flowers Was lower than in control at pH6 determination condition although it Was
  higher at pHlO after rehydration．A new kind of endopeptidase might be induced by the treatment．
  
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• Embryogenic Callus Induction and Plant Regeneration of Zoysia japonica
• Li Ruifen;Zhang Jingyuan;Zhao Maolin
• Acta Horticulturae Sinica. 2003, 30(03): 355-357.
• Abstract ( 1378 ) HTML ( 1155 ) PDF (156KB) ( 1155 ) 　　　
• Mature caxyopses of two materials of Zoysia japonica Stend．coilected in diferent habitats were used as initial explants for callus induction. MS containing 2.0 mg/L 2,4-D induced calli at the relatively higher percentage of 31.2％-42.1％. Inclusion of 6-BA in callus induction med ium with various levels of 2,4-D made an important role for non-embryogenic calli changing into embryogenic calli．The embryogenic calli of higher percentage were induced by a combination of 2.0 mg／L 2,4-D and 0.1 mg/L 6-BA．The rates of shoot regeneration and rooting were different in different regeneration medium. The rates of shot regeneration and rooting were 46.8％-48.1％ at the level of 0.1 mg/L 2,4-D.
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研究简报

• Studies on Water and Membrane Lipid Peroxidafion of Cut Peony Flowers after Storage
• Zang Yanqing;Liu Yan
• Acta Horticulturae Sinica. 2003, 30(03): 357-357.
• Abstract ( 1215 ) HTML ( 771 ) PDF (49KB) ( 771 ) 　　　
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研究报告

• Studies on Virus-free and Rapid Reproduction in Cultural Plant of Gladiolus

  and Its Effect
• Li Changyu;Guo Taijun;Jiao Peijuan;Zhang Yafeng;Du Lingge
• Acta Horticulturae Sinica. 2003, 30(03): 358-360.
• Abstract ( 1628 ) HTML ( 1310 ) PDF (138KB) ( 1310 ) 　　　
• A study on tissue culture of gladiolus hybridus of ‘Chenghongjiao’ and ‘Ziyinghua’ was carried out in MS medium with diferent phyto-hormone BA，KT and NAA．A MS medium supplemented with BA 0.5 mg/L+NAA 0.5 mg/L+KT 1 mg/L was for the regenerated and 1/2MS medium without hormone was for bulbs forming．The three main viruses of TMV，CMV and TVY in gladiolus were removed from cultural plant of gladiolus in the regenerated medium through 5-20 mg／L concentration of ribavirin，treatment of high temperature 38-40℃

  and 2 times of meristem-tip culture．
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综述

• Physiological Mechanism of 1-MCP in Delaying Fruits Senescence
• Li Fujun;Yang Hongqiang;Zhai Heng;Shu Huairui
• Acta Horticulturae Sinica. 2003, 30(03): 361-365.
• Abstract ( 1622 ) HTML ( 3184 ) PDF (248KB) ( 3184 ) 　　　
• 1-MCP is an effective ethylene action inhibitor and could delay the senescence， improve the storage quaity, promote the shelf life and affect both the flavors and physiology disorders in postharvest fruits. 1-MCP also could regulate some related some related enzyme activity and related gene’s expression in fruit senescence. This paper reviewed these previous studies on the effects and physiological mechanism of 1-MCP in delaying fruit senescence, In addition, the potential with an emphasis on applications of the research in this area are proposed.
  
  
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问题与讨论

• The Course ot Watermelon Breeding and Germplasm Bases of Watermelon Cultivars in USA
• Wang Haobo;Wang Ming;Gao Xiuwu;Yu Zengliang
• Acta Horticulturae Sinica. 2003, 30(03): 366-369.
• Abstract ( 1695 ) HTML ( 2605 ) PDF (191KB) ( 2605 ) 　　　

• The history of watermelon breeding during 1870-2001 in the USA was reviewed. The course of watermelon breeding in the USA was divided into three stages. The pedigrees of ninety-one crossbred and inbred cultivars of two hundreds thirty-five released watermelon cultivars were analyzed, and the genetic contribution of their direct parents and ancestor parents were calculated. Their core parents with main genetic contribution were selected out. How to introduce and utilize these core germplasm was discussed.

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新品种

• A New Pear Cultivar—— ‘Huagai rang’
• Liu Fengiun;Zhang Kaibin;Zhang Zhihong
• Acta Horticulturae Sinica. 2003, 30(03): 370-370.
• Abstract ( 1594 ) HTML ( 1172 ) PDF (51KB) ( 1172 ) 　　　
• ‘Huagai King’， a tetraploid pear cultivar, was selected from the bud mutation of pear cultivar ‘Huagai Pear’. It maintains the excellent characteristics of ‘Huagai Pear’, super fruit quality，high yield and strong cold resistance. The most outstanding characteristic of ‘Huagai King’ is its enormous fruit size，and the average mass of 'Huagai King’ was more than two time of the average mass of ‘Huagai Pear’ and the biggest one reached 250 g.
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• A New Early Ripening Pear Variety ‘Zaokui’
• Wang Yingtao;Li Yong;Li Xiao;Liu Guosheng;Han Yanxiao
• Acta Horticulturae Sinica. 2003, 30(03): 371-371.
• Abstract ( 1443 ) HTML ( 1126 ) PDF (52KB) ( 1126 ) 　　　
• The early ripening pear variety ‘Zaokui’ is the progeny of hybrid of ‘Xuehua Pear’ and ‘Huanghua Pear’. The fruit has a oval shape with green-yellow skin; The average fruit mass is 264.5 g，the largest fruit mass is 500 g；The fruit fresh is fine and crisp with sweet flavorance；The soluble solid content is 12.6％, has
  a top quality．The fruit ripen in early August with an ideal productivity and highly resistant to pear scab.
  
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• A New Chestnut Variety ‘Jiejiehong’
• Chen Suchuan;Xiao Zhengdong;Huang Guofu;He Dinghua;Xu Jianmin;Xiao Bin
• Acta Horticulturae Sinica. 2003, 30(03): 372-372.
• Abstract ( 1430 ) HTML ( 1069 ) PDF (73KB) ( 1069 ) 　　　
• ‘Jiejiehong’，a new variety selected from the chestnut resources of Anhui province．It has strong ability to produce more pistillate flowers and strong resistance to pruning and detrimental conditions，and that it also has many other advantages such as early fruit，good quality and so on．
  
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• ‘Jiaxian Youzao’—— A New Drying Variety of Zizyphus jujuba Mill.
• Li Xingang;Tong Jinxia;Zhang Tongqing
• Acta Horticulturae Sinica. 2003, 30(03): 373-373.
• Abstract ( 1417 ) HTML ( 988 ) PDF (73KB) ( 988 ) 　　　
• ‘Jiaxian Youzao’，a new drying variety with late repening，selected from ‘Zhongyang Muzao’ jujube orchards in Jiaxian county，Shaanxi. The fruit is oval or column shape，red-black colour, and middle large, the average fruit mass is 12.3 g，maximum fruit mass is 19.6 g．It ripens in early-middle October in north Shaanxi．The fruit is uniform， attactive, good quality fruit, and applicable to dry. Its cultivated region is in north China with the cool temperature (9-11 ℃) and less rainfall (annual rainfall of 390-500 mm).
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• A New Banana Variety—— ‘Zhangiiao 8’
• Guo Jianhui;Huang Xidong;Li Zhiwei;Lin Qie;Cai Enxing;Huang Hongnian
• Acta Horticulturae Sinica. 2003, 30(03): 374-374.
• Abstract ( 1606 ) HTML ( 1019 ) PDF (49KB) ( 1019 ) 　　　
• ‘Zhangiiao 8’ is a fine promoted variety，which is breeded and selected from mutated plants in vitro tube-buds of Fujian ‘Taiwan Grand Cavendish’ irradiated by ⁶⁰Co γ ray．The variety grows up with characters of its pseudostem light green，petiole longer and plant type straighter，morever without pappus on its fruit axes．Compared with control, the variety has an over 10％ increase of yield， finer appearance of fruit ears， longer and heavier fruit fingers．For richer in vitamin C and higher of TSS/TA，the variety tastes more flavorous．
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• A New Small-size Seedless Watermelon ‘Xuefeng Xiaoyuhong’
• Deng Dacheng;Sun Xiaowu;Zuo Puyang;Liu Yuehua;Xie Xiangdong
• Acta Horticulturae Sinica. 2003, 30(03): 375-375.
• Abstract ( 1682 ) HTML ( 967 ) PDF (49KB) ( 967 ) 　　　
• ‘Xuefeng Xiaoyuhong’ is a early small-size seedless watermelon hybrid．The variety is suitable for protected production in early spring and late autmnn,and also suitable for all waterm elon planting -aIeas， It is globe in shape with smooth dark-green rind an d hidden stripes. It is of high edible quality with sweet， crisp and juicy red flesh．Central sweetness is 12％-13％. Fruit inass is 1.5-2.5 Kg ．It’s resistant to disease．
  
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• A New Summer Squash Hybrid for Protected Cultivation ‘Yindie 1’
• Cheng Yongan;Zhang Enhui;Xu Zhongmin;Wang Yanni;Ma Qingshan;Fu Qiaoling
• Acta Horticulturae Sinica. 2003, 30(03): 376-376.
• Abstract ( 1514 ) HTML ( 868 ) PDF (77KB) ( 868 ) 　　　
• A new mid-early-maturing summer squash hybrid ‘Yindie 1’ has been released. Its period of duration is about 50 days．Young fruit is unidentified fly object(UFO) shaped and wave of fruit edge with white coat， 15-25 cm in diameter, 5—8 cm in thickness．Average fruit mass is 200—400 g．The fruit abounds
  in Vitamin C．The flesh of fruit is thick and seed is little． It is with good vigor and resistance to virus and cool and weak light. Old-maturing fruit may be as ornamental plant.
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• A New Vegetable Soybean with High Yield and Quality ‘Xiafeng 2008’
• Mao Weihai;Bao Chonglai;Sun Lixia;Hu Tianhua;Hu Qizan
• Acta Horticulturae Sinica. 2003, 30(03): 377-377.
• Abstract ( 1362 ) HTML ( 1057 ) PDF (46KB) ( 1057 ) 　　　
• ‘Xiafcng 2008’ is a new vegetable soybean with high quality and yield planting in Summer. It can be harvested in 80 days after pollinating. The variety has a heat tolerance, exuberant vitality, durable much fertilizer and lodging-resistant. It has fine green pod with good quality and high marketable percentage. The yield is about 7.5-8 t/hm².
  
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