研究论文

• Changes of Physiological Characteristic on Photosynthesis in Malus Seedling Leaves during Water Stress
• Cao Hui;Xu Xuefeng;Han Zhenhai;Wang Xiaowei;Guo Tuqiang
• Acta Horticulturae Sinica. 2004, 31(3): 285-290.
• Abstract ( 1608 ) HTML ( 1544 ) PDF (592KB) ( 1544 ) 　　　
• Changes of physiological characteristic on photosynthesis in Malus seedling leaves during water stress were studied with polyethylene glycol(PEG 6000)treatment．The results were as follows：Under water stress．the binding of Chl-Pro was loosened with the changes of time and intensity of water stress．Net photosynthesis rate(Pn)，stomatal conductance(Gs)decreased，intercellular CO₂ concentration (Ci)increased，then decreased．The activities of SOD，CAT increased in the early period，then decreased．The 0₂^-. generating rate and contents of H₂0₂ increased during water stress，the content of antioxidants carotenoid(Car)、ASA decreased．The changing extents of these parameters in the drought tolerant variety sieversii were lower than the drought resistive variety M.hupehensis． Under water stress．there are high negative significant correlation between Pn and Chl-Pro O ，H₂O₂ in M.hupehensis (Pamp．)Reld．and M.sieversii (Ledeb．) R0em． seedlings． With the duration of water stress。the excessive accumulati0n 0f 0₂^-.。H₂O₂nduced the nonstomatal limitation．It played key role in the decline of the photosynthetic rate.
• Related Articles | Metrics

• Relationships between Distribution of Relative Light Intensity and Yield and Quality in Diferent Tree Canopy Shapes for 'Fuji' Apple
• Wei Qinping;Lu Renqiang;Zhang Xianchuan;Wang Xiaowei;Gao Zhaoquan;Liu Jun
• Acta Horticulturae Sinica. 2004, 31(3): 291-296.
• Abstract ( 1921 ) HTML ( 1864 ) PDF (694KB) ( 1864 ) 　　　
• The diference of distribution in relative light intensity.characteristic of canopy space in fruit yield and quality，and their relationships were studied with the four diferent tree canopy shapes in the‘Fuji’ apple orchards.The results howed that the relative light intensity in excess 30% was high and the light istribution in every layers were uniform and parallel arrangement in open center shape．Th e canopy volume ratio of relative light intensity insuficient to 30% were 23.33% ，25.95%，32.86%，40.24%and output were 47.4，46.6，44.9，39.5 t/hm in open center shape，the reform open center from small canopy shape by one year，the reform open center from small canopy shape by two years and small canopy shape respectively．The open center shape were lower 17% in useless light space，higher 20% in output and greater in fruit coloration,solubility solid matter，solubility sugar，fruit shape index and ratio of sugar and acid than small canopy shape.The regression equations in relationships between factors of quality and relative light intensity were set up to obtain optimum range of relative light intensity for good quality in ‘Fuji’ apple．
• Related Articles | Metrics

研究简讯

• The Studies of the Tissue Culture of Mandevilla sanderi
• Li Yonghong;Zeng Daxing;Xie Lijuan
• Acta Horticulturae Sinica. 2004, 31(3): 296-296.
• Abstract ( 1608 ) HTML ( 868 ) PDF (350KB) ( 868 ) 　　　
• Related Articles | Metrics

研究论文

• Soil Adaptability of Grape in the Dry Valley of the Upper Reaches of Minjiang River
• Xiang Shuang;Liu Shiquan;Chen Qingheng;Ding Jianlin;Qiao Yongkang
• Acta Horticulturae Sinica. 2004, 31(3): 297-302.
• Abstract ( 1630 ) HTML ( 1104 ) PDF (256KB) ( 1104 ) 　　　

• Soil suitability for table and wine grapes in the dry valley of the upper reaches of Minjiang River was assessed．Based on the favorable climate．1eaf analysis and soil diagnosis were conducted．The results demonstrated that the differences of leaves nutrient attributed to the grape varieties． It iS the common characteristic for low nitrogen，high·middle phosphorus，high potassium and iron and zinc deficiency in this area． Th is results from the poor N and the abundant P and K in the soil on the whole． Moreover．the pH, texture and depth of soil meet the needs of vineyaed establishment． Thus,integrated analyses showed that it is necessary of equilibrium fertilization．s0il and water conservation，slope and soil amelioration for vineyard．

• Related Articles | Metrics

• Embryo Rescue and Identification of Hybrids between Sweet Cherry and Chinese Cherry
• Liu Huanfang;Chen Xuesen;Duan Chengguo;Yang Honghua;Feng Baochun
• Acta Horticulturae Sinica. 2004, 31(3): 303-308.
• Abstract ( 1895 ) HTML ( 1299 ) PDF (437KB) ( 1299 ) 　　　
• Interspecific hybridization was carried out with selecting sweet cherry as female parent and Chinese cherry as male parent．Embryo rescue system of hybrids were studied and identification of them Was also made．The results showed that：(1)setting percentage was highest when ‘Ehon’was female parent；embryo abortion happened 4 weeks after pollination in ‘Ehon’，3 weeks in ‘Napoleon’and ‘Hongdeng’；(2)1／2 MS medium supplemented with BA 2 mg／L，IAA 1 mg／L and vitamin C 10 mg／L was the optimal medium for germination and growth，1／2 MS medium supplemented with BA 2 mg／L，IAA 1 mg／L，AA 0．1 mg／L and vitamin C 1 0 mg／L Was the optimal medium for multiplication，1／2 MS medium supplemented with IBA 0．2 mg／L and vitamin C 10 mg／L was the optimal medium for rootage；(3)S-allele specific PCR、RAPD and leaf shape analysis showed that they were real hybrids．
• Related Articles | Metrics

• Studies on Genetic Diversity and Phylogenetic Relationship of Cucumber(Cucumis sativus L．)Germplasm by AFLP Technique
• Li Xixiang;Zhu Dewei;Du Yongchen;Shen Di;Kong Qiusheng;Song Jiangping
• Acta Horticulturae Sinica. 2004, 31(3): 309-314.
• Abstract ( 1770 ) HTML ( 1582 ) PDF (599KB) ( 1582 ) 　　　
• In this study，genetic diversity in 70 accessions of Cucumis sativus L．from diferent countries or regions was assessed using AFLP markers，and the genetic relationship among the accessions was revealed by cluster analysis．Eight pairs of AFLP informative primers generated 425 scorable bands in total．66％ of which were polymorphic．The average expected heterozygosity of AFLP loci among all accessions Was 0.376．The heterozygosity among Chinese accessions Was higher than that am ong exotic accessions．C．sativus L．vat． xishuangbannanesis Qi et Yuan and India wild germpalsm had some special loci that were not present in cultivars． rhe special loci in China cuhivars are more than that in the germplasm from America and Europe．rhe exotic germplasm also has some special loci that was absent in Chinese germpalsm．Three groups were clustered based on AFLP markers，namely C．sativus L.Var.xishuangbannanesis Qi et Yuan group，C．sativus Var．hardwicki group and cuhivars group．C．sativus L．Var．xishuangbannanesis Qi et Yuan Was the farthest from cuhivars．India wild cucumber was the farther from cuhivars．At certain distance，Chinese cultivars could be diferentiated from the exotic germplasm． Most south China ecotypes and North China ecotypes could be grouped into diferent subgroups but a few diferent ecotypes went into a Sal’ne subgroup．These results are beneficial for US to use various target variants to widen the genetic background of cucumber cultivars．
• Related Articles | Metrics

• Studies on the Anatomical and Cytological Characteristics of Blunted with Blossom Cucumber
• Li Zhiying;Li Xingguo;Xu Li;Zheng Chengchao
• Acta Horticulturae Sinica. 2004, 31(3): 315-320.
• Abstract ( 1590 ) HTML ( 1135 ) PDF (1231KB) ( 1135 ) 　　　
• In order to reveal the characteristics of the“blunt with blossom”cucumbem (BBCs)．we investigated the anatomical and cytological characteristics of the young stems and shoot tips of the BBCs by light microscopy(LM)，transmission electron microscopy(TEM)and scanning electron microscopy(SEM)procedures．In BBCs plants。the node number。internode length and the stem diameter in shoot tops all reduced，and the elongation and expansion of the stem cells were inhibited．In contrast，the wall thickness of the collenchyma cell,the number of the vessel bundles．and the number of mature vessels in young stem increased respectively．Meanwhile，the shoot apical meristem cells avacuoled。and the nuclear envelope，tonoplast，plastid envelope and endoplasmic reticulum dissolved partially．In the studies on the role of BBCs。two positive cDNA bands were obtained through mRNA differential display．Northern blot analysis showed that one cDNA fragment homologous to the chloroplast ATPase subunit III Was down regulated and the other cDNA fragment homologous to the arginine deearboxylase(ADC)was up regulated in BBCs．
• Related Articles | Metrics

• Promotion of Photosynthesis by 5-Aminolevulinic Acid (ALA)during and after Chilling Stress in Melon Seedlings Grown under Low Light Condition
• Wang Liangju;Jiang Weibing;Huang Baojian
• Acta Horticulturae Sinica. 2004, 31(3): 321-326.
• Abstract ( 1606 ) HTML ( 1604 ) PDF (912KB) ( 1604 ) 　　　
• Irrigation with exogenous 5-aminolevulinic acid (ALA) significantly promoted leaf net photosynthetic rate(Pn)of melon(Cucumis melo L.‘Ximiya 1’)seedlings grown under low light irradiation,as well as the plants treated by 8 ℃ chilling stress.The leaves of the plants treated with 10 mg·L^-1 ALA solutions showed hi gher apparent quantum yield(AQY)，carboxylation eficiency(CE)，chlorophyll(especially chlorophyl b)content，dark respiration and bigger stomata aperture but lower respiration under light.Furthermore,after seedlings were transferred to 8℃ chilling for 4 hours and then recovered at 25-30℃ for 2 hours，the photosynthesis of the control leaves was only 12%-18% of that before chiling,while that in the ALA-pretreated leaf was 22%-38%.which would further recovered in 20 hours to 76%-101% of the control before chilling treatment.If the seedlings were chiled for 6 hours．the control plants all died while the ALA-pretreated ones only showed a few injury symptoms in the leaf margin．The analysis also showed that the soluble sugar content of leaves in ALA-retreated plants Was very significantly higher than that of control，which might be partly responsible for the higher chiling tolerance．The evidence presented here sugested that ALA treatment might promote low light tolerance and chilling tolerance of plants.
• Related Articles | Metrics

• Inheritance of the Chlorophyll Content in Immature Fruit Color of Cucumber
• Sun Xiaolei;Wang yongqiang;Wang Bing;Gu Sanjun;Wang Zhifeng;Cao Qiwei
• Acta Horticulturae Sinica. 2004, 31(3): 327-331.
• Abstract ( 1946 ) HTML ( 1188 ) PDF (192KB) ( 1188 ) 　　　
• In this paper inheritance of the chlorophyll content in immature skin of cucumber was investigated by applying the major gene plus poly—genes model of quantitative traits to a joint analysis of multi—generations(P₁、F₁、P₂、B₁、B₂and F₂)．The results showed(1)the immature skin color of cucumber Was mainly controlled by nuclear genes.(2)the chlorophyll content in immature skin of the cross of HYBP×JNQHG Was controlled by two major genes with additive-dominance effects plus polygenes with additive-dominance effects(the E-2 model)．(3)heritability values of the major genes of B₁、B₂ and F₂ population were estimated as 83.94% ，62.12%and 86.98%respectively,while polygenes of those were 7.23%,18.15%and 5.86%.The results demonstrated that the chlorophyll content level in immature skin of the cross was mainly controlled by two major genes mixed with the efect of polygenes．The estimated additive efects of the first major gene(d_a )was much higher than that of the second one db．There were obvious diferences in genetic efects between the two major genes．The efect of db was only about 35.51% that of d_a，indicating that
  the contributions to the chlorophyl content level of the two major genes were not equal in the cross．The dominant efect of the two major genes，h_a and h_b were 2.7762 and 2.3392 respectively．
• Related Articles | Metrics

• Cloning of Flower Development Associated MADS box Gene Fragments in Lilium
• Zhang Yun;Liu Qinglin;Ouyang Qing;Cai Wenqi
• Acta Horticulturae Sinica. 2004, 31(3): 332-336.
• Abstract ( 1555 ) HTML ( 1334 ) PDF (1498KB) ( 1334 ) 　　　
• LfMADS 1-3，three MADS box cDNA fragments associated with flower development were cloned from Lilium using a RT-PCR based cloning method．LfMADS1 and LfMADS3 are new MADS box genes in lilies．which have not been reported before．They showed high sequence homology with AG and SEP homo1ogs, respectively．The deduced amino acid sequence of LfMADS2 shared 98.99％ identity with the reported LfMADS2 ,which suggested that they might be the same gene．
• Related Articles | Metrics

• Starch Metabolism and Sprouting of Bulb in Lilium davidii var. unicolor Stored at Diferent Cold Temperatures
• Sun Hongmei;Li Tianlai;Li Yunfei
• Acta Horticulturae Sinica. 2004, 31(3): 337-342.
• Abstract ( 1824 ) HTML ( 1493 ) PDF (252KB) ( 1493 ) 　　　
• Carbohydrate contents were markedly diferent according to various storage temperatures，storage durations and parts of bulb．Bulbs stored at 6℃ had the highest starch level in bud and the lowest starch level in basal plate.However,bulbs stored at 10℃ had the opposite trend．Starch content in scales declined along with the decrease of storage temperature．Soluble sugar content in all parts of bulb and amylase activity of bud or scales decreased along with inerease of temperature．but showed an order of 6℃ >10℃ >2℃ in
  basal plate．An extremely negative correlation was observed between starch content and am ylase activity．The first 34 days of cold storage was the most active period of carbohydrate metabolism，which the bud developed rapidly．Total soluble sugar and amylase activity increased in relation with the decrease of starch．The extent of change in bud and basal plate was greater than that in scales，and in exterior scales Was greater than in other scales.The significant interaction between temperature and duration afected carbohydrate metabolism and sprouting of bulb．The results indicated that storage at 2℃ for 101 d Was the best treatment for the dormancyrelease．
• Related Articles | Metrics

• Efects of GA₃，Ca²⁺ on Anthocyanin and Phenylalanine Amonia Lyase in Stem of Anthurium andraeanum
• Xia Chunhua;Cai Shiying
• Acta Horticulturae Sinica. 2004, 31(3): 343-346.
• Abstract ( 1740 ) HTML ( 1288 ) PDF (178KB) ( 1288 ) 　　　
• Efects of the environmental factors on the anthocyanin content in spathes and of improving flower quality with Ca²⁺ ,GA₃ treatments under weak light were investigated in Anthurium andraeanum．The resuits indicated that weak light was the main reason resulting in the lower anthocyanin content．Ca²⁺ (5.4 mmol· L^-1)，GA₃(1.16 mmol·L^-1)or Ca²⁺ +GA₃，respectively，significantly increased the anthocyanin content in spathes and the phenylalanine amonia lyase(PAL)activity in stem of Anthurium under weak light．It is found that the calmoduiin contents and PAL activity in vitro stem of Anthurium were inhibited th diferent inhibitors of calmoduiin，RNA and protein，and the probable mechanisms of Ca²⁺ ，GA₃were discussed．
• Related Articles | Metrics

研究报告

• Relationship between Coloration and Antioxidant Enzymes in ‘Fuji’ Apple Fruit
• Qie Guangfa;Liu Junhua;Dong Xiaoying;Li Peihuan;Liu Chenglian;Wang Yongzhang;Yuan Yongbing
• Acta Horticulturae Sinica. 2004, 31(3): 347-349.
• Abstract ( 1761 ) HTML ( 1753 ) PDF (139KB) ( 1753 ) 　　　
• Apple(Malus×domestica Borkh‘Fuji’)fruit was used to study the relationship between coloration and antioxidant enzymes．The contents of pigments and the activities of antioxidant enzymes in bagging and non-bagging fruits were measured at different time during ripening period．The results showed that the
  change of anthocyanin content was closely correlated with the activities of antioxidant enzymes．The activities of SOD and POD were high before coloration and then decreased obviously as red color developed．Bagging inhibited the activities of SOD and POD,When fruit was bag-removed in color-turning stage．the activities of SOD and POD increased sharply in the first 2 days．then dec reased significantly with the accumulation of anthocyanin from 2 days after bag．remova1．In addition，LOX activity Was closely corelated with the change of anthocyanin content．LOX activity appeared highest at 2-3 weeks before the color-turning stage，and declined with the accumulation of an thocyanin.
• Related Articles | Metrics

• Fe-efficient Traits and Their Relationships to Chlorisis Index in Genus Malus
• Li Yinghui;Han Zhenhai;Xu Xuefeng
• Acta Horticulturae Sinica. 2004, 31(3): 350-352.
• Abstract ( 1507 ) HTML ( 1414 ) PDF (145KB) ( 1414 ) 　　　
• Relationship between a part of correlative traits and chlorisis index under Fe-eficiency stress were studied with Fe-efficient Malus xiaojinensis，M．baccata and an interspecific F₁ hybrids of xiaojinensis×baccata．The results showed that the correlation coemcients(r)among the chlorophyll concentrations,number of roots，pH value as percentage of control with chlorisis index were -0.8218， -0.8775,-0.2924 respectively．These indicates that the higher the chlorisis index of lines in the hybrids，thus，the more obviously the chlorophyll concentrations decreased，the less the no. of roots increased，the higher the pH value decreased．
• Related Articles | Metrics

• Studies on the Embryogenesis of Persimmon
• Zhong Yun;Liu Yong;Jiang Nonghui;Liu Shanju
• Acta Horticulturae Sinica. 2004, 31(3): 353-356.
• Abstract ( 1442 ) HTML ( 1191 ) PDF (468KB) ( 1191 ) 　　　
• The embryogenesis of persimmon was mainly studied on the varieties of ‘Kazusa’ and ‘Gaoan Fangshi’．Materials were dehydrated，embedded and cut in Paraffin wax．Th e results were as follows：Th eir ovules Was anatropous，biteguminous and tenuinucellate，archesporium one-celled and functioning directly as the megaspore mother cell．The tetrad ofmegaspores Was linear shaped，with the first cell from the chalazal end being functiona1．The development of the embryo sacs belongs to the ‘polygonum’type．The female gametophyte consists of egg apparatus，central cell and three antipodal cells at the mature embryo sac stage．The filiform apparatus of each synergid was at the micropyle end．The double fertilization belongs to the premitotic type of syngamy．Three antipodal cells were triangularly arrayed．The embryo develops to confornl the‘Solanad’type．Zygote began to divide after anthesis about two weeks．Th e development of endosperm conforms to the nuclear type．The primary endosperm nucleus began to divide 3 days after anthesis．Endosperms began to undergo cellular endosperm stage at the early globular emb ryo stage and almost completed at the cotyledonary emb ryo stage．Many abnorm al phenomena were observed during the development of embryo: Endosperm abortion and the division of zygote did not occur，etc．
• Related Articles | Metrics

研究简讯

• Changes in Nucleic Acid an d Protein Contents at Diferent Developmental Stages in Male an d Female Flower of Cucumber
• Chen Xuehao;Chen Yanping;Cao Beisheng
• Acta Horticulturae Sinica. 2004, 31(3): 356-356.
• Abstract ( 1725 ) HTML ( 1077 ) PDF (47KB) ( 1077 ) 　　　
• Related Articles | Metrics

研究报告

• Development of Flower Organs in Sweet Cherry in Shanghai Area
• Wang Shiping;Yuan Caijuan;Dai Yunting;Shu Huan;Yang Tianyi;Zhang Caixi
• Acta Horticulturae Sinica. 2004, 31(3): 357-359.
• Abstract ( 1745 ) HTML ( 1263 ) PDF (376KB) ( 1263 ) 　　　
• In order to explore the reason for poor fruit set in sweet cherry grown in Shanghai area，the development of flower organs was investigated with 5-year-old sweet cherry(Prunus avium L．)trees which were planted in tlle research orchard of Shanghai Jiaotong University．Results showed that floral diferentiation and appearance of stamen and pistil during their development were normal for sweet cherry in Shanghai area.The microscopic observations revealed that poNen developed well and polen germination rate reached about
  50％.However．98.2％ of ovary failed to develop，among which tllere was 26.3％ of ovaries witllout ovule, and 71.9％ of ovaries witll ovule but witllout embryo sac.The result indicated tllat undeveloped ovule and embryo sac are tlle main reason for poor fruit set of sweet cherry in Shanghai area．
• Related Articles | Metrics

• Ooning of Nramp Gene Fragment Being Relating to Related to Resistance of Iron Deficiency Stress from Malus xiaojinensis
• Qi Jinliang;Han Zhenhai;Yin Liping;Xu Xuefeng;Zhang Lianzhong
• Acta Horticulturae Sinica. 2004, 31(3): 360-362.
• Abstract ( 1771 ) HTML ( 1054 ) PDF (428KB) ( 1054 ) 　　　
• To initially study the role of Nramp gene in resisfng iron deficiency stress，we analyzed this gene in Malus xiaojinensis Cheng et Jiang by using blot hybridization technique with Nramp gene fragment of wheat as probe．Southern blot result suggested that there was Nramp gene in the genome of Malus xiaojinensis.Northern blot confirmed that transcription ofthe Nramp gene is induced in both roots and leaves and strengthened in leaves by iron stress.A 752 bp fragment of Nramp gene was obtained by PCR from the genomic DNA，
  which has 83％ identities to OsNramp3 of rice in amino acid leve1．The results lead to the prediction that Nramp gene of Malus xiaojinensis may play a crucial role in resisting iron stress．
• Related Articles | Metrics

• Studies of Energy Spectrum Analysis on the Absorption and Distributive Laws of Nutrient Elements in Sucking Roots of Ginkgo biloba L．
• Ling Yuping;Zhou Weidong;Chen Peng;Chen Qimiao;Xiong Zuoming
• Acta Horticulturae Sinica. 2004, 31(3): 363-366.
• Abstract ( 1614 ) HTML ( 1089 ) PDF (161KB) ( 1089 ) 　　　
• Based on energy spectrum analysis on absorbing laws to nutrient elements for tissue of sucking roots．the results were as follows：1．It showed two peaks which appeared at the 4—6 hour and the 10-24 hour after treatment by nutrient liquid respectively for root to absorb nutrient elements：2． The absorption laws and distribution of nutrient elements in different root tissues of Ginkgo were relative to the types of element and ion in culture liquid．and to characters of requirement of root system to nutrient element：3． There were more accumulate of potassium and sodium in root system of Ginkgo，which showed that Ginkgo biloba Was a plant of strong metabolism and salt endurance．
• Related Articles | Metrics

• Studies of Four Kinds of Anthocyanin in Fruit of Diferent Genotype of Vaccinium ssp． plants
• Li Yadong;Meng Fanli;Zheng Yi'nan;Su Xiaotia
• Acta Horticulturae Sinica. 2004, 31(3): 367-368.
• Abstract ( 1718 ) HTML ( 1672 ) PDF (80KB) ( 1672 ) 　　　
• The content of four kinds of anthocyanin were determined by RP—HPLC in 2 wild species and 3 cuhivars．Th e result showed that the order of total content of anthocyanin was Chignecto，CA-206，NB-3.Bog bilberry and Lingonberry. Moreover，the category and content were different in fruit of difierent species and cuhivars，Cyandine-3-galactoside was an usual anthocyanin，but cyandine-3-glucoside didn’t exsit in Bog bilberry and Lingonberry，the fruit of Lingonberry contained one of four kinds of anthocyanin．
• Related Articles | Metrics

• Efects of Rare-earth Elements Cerium on ‘Red Globe’ Grape Plantlets in Vitro
• Xue Jianping;Xu Min;Zhang Aimin
• Acta Horticulturae Sinica. 2004, 31(3): 369-371.
• Abstract ( 1469 ) HTML ( 1022 ) PDF (281KB) ( 1022 ) 　　　
• The effect of different concentration Ce(NO₃)₃ from 1 to 100 mg·L^-1 on the growth of‘ Red Globe’grape plandets in vitro were investigated．The results indicated that the optimum concentration of Ce(NO₃)₃ which promoted growth of plantlets was 1 to 10 mg·L^-1．The growth performance of plandets in vitro as follows：The number and length of roots increased obviously．Fresh and dry matter of roots，stems and leaves as well as the contents of chlorophyll also increased．Survival rate of transplantation increased due to roots activity which could improve mineral nutrition absorption ability of plantlets．While，high concentration of Ce(NO₃)₃ (100 mg·L^-1 )in medium obviously inhibited plantlets growth．
• Related Articles | Metrics

• The Possible Relationship between the Regeneration Capacity and Reactive Oxygen Species in the Strawberry Calli
• Tian Min;Han Ning;Bian Hongwu;Zhu Muyuan
• Acta Horticulturae Sinica. 2004, 31(3): 372-374.
• Abstract ( 1678 ) HTML ( 1114 ) PDF (267KB) ( 1114 ) 　　　
• Three types of callus，which present different regeneration capacity，were established in the tissue culture of strawberry．The Ⅱand Ⅲ types of callus possessed higher regeneration capacity while I type of callus had much lower．In the freshly induced calli，the Ⅱand Ⅲ types of callus had 5-fold and 9-fold higher contents of intracellular H₂0₂，and 3-fold and 4-fold higher level of 0₂^-.，compared with the I type of callus,respectively．Also，the calli Ⅱand Ⅲ had much higher activities of antioxidant enzymes than the callus I. During the subculture，H₂0₂level increased gradually and 0₂^-. rapidly，while the activities of antioxidant enzymes gradually declined．The ability of expression of totipotency in calli decreased，especially the callus I lost the ability after 12 week subculture．The results indicated that the reactive oxygen species might play a dual role in the regeneration of strawberry calli．On the one hand，a certain level of ROS may have a positive effect on the regeneration，and on the other hand，high level of ROS is inhibitory for the expression of totipotency in calli．
• Related Articles | Metrics

• Changes in Carbohydrate Content of Stem Apices and Leaves during Floral Bud Difierentiation in Radish (Raphanus sativus L．)
• Wang Bingliang;Deng Jianying;Zeng Guangwen
• Acta Horticulturae Sinica. 2004, 31(3): 375-377.
• Abstract ( 1663 ) HTML ( 1895 ) PDF (578KB) ( 1895 ) 　　　
• Winter cultivar ‘Yidianhong’ and spring cuhivar ‘Duanye 13’were used to study the carbohydrate content of stem apices and leaves during the progress of floral bud differentiation． Germinated radish seeds were subjected to chilling at 5℃ for 20 days．After low temperature treatment．all seedlings were transplanted into glasshouse under warm (>15℃)and long photoperiod (16 h)growth condition. Amounts of total soluble sugars，sucrose and starch were assayed during the various floral bud differentiation stages．The following conclusions were drawn：(i)111e amounts of total soluble sugars．sucrose and starch during the floral bud differentiation，either in stem apices or in leaves。showed a similar tendency between the spring cuhivar and the winter cuhivar．(ii)111e total soluble sugar content increased during the initial stages of floral bud differentiation and then decreased，while the sucrose and starch content increased steadily until floral bud differentiation was completed． (iii)The mounts of total soluble sugars．sucrose and starch in leaves were less than that in stem apices．(iV)Total soluble sugar content in the winter cuhivar was much higher than that in the spring cuhivar during the initial stage of floral bud differentiation，but the sucrose and starch content in the winter cuhivar were lower than that in the spring cultivar during the entire floral bud differentiation．
• Related Articles | Metrics

• Efects of Diferent Cadmium Levels on the Growth and Antioxidant Enzymes in Brassica campestris L．ssp．chinensis (L．)Maidno
• Sun Guangwen;Zhu Zhujun;Fang Xuezhi
• Acta Horticulturae Sinica. 2004, 31(3): 378-380.
• Abstract ( 1507 ) HTML ( 1143 ) PDF (120KB) ( 1143 ) 　　　
• A hydroponic experiment was carried out to study the effects of different Cd levels on the growth and the activities of antioxidant enzymes in two cultivars of Brassica campestris L．ssp．chinensis(L．) Makino．Cd 1 mg·L^-1 didn’t significantly afect the growth and chlorophyll content of both cultivars (except the shoot dry mass of‘ 'Hangzhou Youdonger’)，Cd 10 mg·L^-1 significantly inhibited the growth and leaf chlorophyll content of both cuhivars(except the root dry mass of‘ 'Huqing 1’)．，nle generation of 0₂^-. and H₂O₂，content increased under Cd exposure，and mal0ndialdehyde(MDA)accumulated．The activities of catalase (CAT) and peroxidase (POD) of‘ Huqing 1’ and ascorbate peroxidase (APX) of ‘Hangzhou Youdonger’were significantly increased at Cd 1 mg·L^-1．Cd 10 mg·L^-1 significantly decreased the activities of superoxide dismutase (SOD)，CAT and APX，and significantly enhanced t}Ie activities of dehydroascorbate reductase (DR) and glutathione reductase (GR) in both cuhivars．
• Related Articles | Metrics

• Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L. and Brassica olereacea L．
• Wang Maoguang;Wu Junyan;Wu Nengbiao;Zhu Liquan;Wang Xiaojia
• Acta Horticulturae Sinica. 2004, 31(3): 381-383.
• Abstract ( 1413 ) HTML ( 946 ) PDF (481KB) ( 946 ) 　　　
• Thioredoxin-h-like protein DNA and c DNA were amplified from genomic DNA and stigma total RNA in Brassica napus L．and B．olereacea L. by PCR and RT-PCR method respectively．Sequence analysis first indicated THL2 gene contained two introns．which were diferent in size between B．napus L. and B．olereacea L. Homologous analysis demonstrated that there was 96％ identity of DNA from B.napus L. and B．olereacea L．．
• Related Articles | Metrics

• Genetic Diversity Analysis of Neo-tuberosum by RAPD Makers in Potato
• Di Hong;Jin Liping;Chen Yili
• Acta Horticulturae Sinica. 2004, 31(3): 384-386.
• Abstract ( 1388 ) HTML ( 1049 ) PDF (422KB) ( 1049 ) 　　　
• With 14 random pfimem，the RAPD analysis was used to determine the genetic relationships between 17 Neo-tuberosum clones and 7 common cuhivars of potatoes(Solaaura tuberosum ssp．andigena)．The results indicated that the Neo-tuberosum clones were with abundant genetic diversity． One hundred and seventy unambiguous bands were observed．in which 130 were polymorphic bands．The average polymorphism rate was 75.26％.Phylogenetic relationships of 24 genotypes were discovered based on phylogenetic tree by use of UPGMA method．which were divided into 4 groups．The Neo-tuberosum and the common cultivars were clustered into different groups respectively．
• Related Articles | Metrics

• Preliminary Study OH Relations between Germination Percentage of Eggplant Seed under Diferent Temperatures and Adult Plant Resistant to Low-tenknerature
• Jing Lijun;Wang Liying;Shi Yao;Liu Wenming;Bao Chongla
• Acta Horticulturae Sinica. 2004, 31(3): 387-388.
• Abstract ( 1985 ) HTML ( 1324 ) PDF (87KB) ( 1324 ) 　　　
• Four materials were used to study the appraisal methods and indexes for eggplant low-temperature ,Index of chiming tolerance was established and 48 eggplant materials were tested by planting in the 2 tunnels under low-temperature actually．The resuhs showed that the germination percentage germ inated for 96 h under 20 ℃ after seed soaking by gibberellin solution 1 μL/Lcould explore eggplant low-temperature tolerance correctly．
• Related Articles | Metrics

• Efects of Start Fertilizers on Growth of Poinsettia
• Jiao Xiaoyan;Sun Jingtong;Guan Chao;Ren Zhiqiang;Liu Huimin
• Acta Horticulturae Sinica. 2004, 31(3): 389-391.
• Abstract ( 1466 ) HTML ( 951 ) PDF (132KB) ( 951 ) 　　　
• This study examined the efects of various start fertilizers on poinsettia growth and nutritional status in different portions of poinsettia．Results of the study show more favorable growth and higher quality of poinsettia grown in fast-release-fertilizer (FRF) than in slow-release-fertilizer (SRF)．The ratio of NO₃^--N
  and NH₄⁺-N in FRF did not significantly affect the growth of poinsettia. Concentrations of N，P，K，Ca，Mg， Cu and Zn in green leaves were comparable to the recommended standards while Fe and Mn concentrations exceeded the levels．
• Related Articles | Metrics

• Efects of Plant Growth Regulators on Preservation of Nymphaea stellata Cut Flower
• Zhang Yuping;Luo Jianxiong;Liu Jingguo
• Acta Horticulturae Sinica. 2004, 31(3): 392-394.
• Abstract ( 1849 ) HTML ( 1496 ) PDF (124KB) ( 1496 ) 　　　
• Efects of diferent plant growth regulators on the vase life and preserving property of Nymphaeft stellata cut flower were studied．The results showed that 50 mg·L^-1.GA₃treatment produced the best resuit for the preserving property of N．stellata cut flower and could extend the vase、ire of N．stellata cut flower highly significantly as compared to the control(distilled water)．It could increase flower diameter，straightened flower stems and prolonged time of the existance of flowers．It also could keep fresh mass of flower stem and retarded the increase of pH value and relative plasmalemma permeability in petals of stellata cut flower.Efects of 10 mg·L^-1 PP₃₃₃ and 10 mg·L^-1 CCC treatment were not significantly as compared to the control1 mg·L^-1 6-BA and 1 mg·L^-1 NAA treatment were not suitable for vase-holding of N.stellata cut flower．
• Related Articles | Metrics

• The Impact of the Diferent Degree of Bamboo Vinegar on the Diferent OrnamentaI Plants Growth
• Zhao Li
• Acta Horticulturae Sinica. 2004, 31(3): 395-396.
• Abstract ( 1648 ) HTML ( 1231 ) PDF (86KB) ( 1231 ) 　　　
• The effect of bamboo vinegar on the growth of some ornamental plants were investigated.The results showed that the bamboo vinegar at 10 μL/cm³ (200-250 fold)concentration significantly were improved the plant growth and increased the chlorophyll contend of the leaf. Besides the adaptiveness of diferent plants to different concentration of bamboo vinegar was diferent too. It was suggested that bamboo vinegar at specified concentration could improve the plant growth and delayed leaf senescence．
• Related Articles | Metrics

• In Vitro Propagation of Phlox subulata L．
• Dai Hongyan;Zhang Zhihong;Gao Xiuyan;Wang Fenglan
• Acta Horticulturae Sinica. 2004, 31(3): 397-399.
• Abstract ( 1843 ) HTML ( 953 ) PDF (378KB) ( 953 ) 　　　
• An eficiency system of propagation jn vitro of Phlox subulata L．was developed. The optimum sterilization of young shoots was 10 minutes with 0.1% HgCl_2. The rate of shoots forming from 1 mm shoot tips reached 83.3%. The multiplication cogmcient of in vitro decapitated shoot was 50％ higher than
  the multiplication coefficient of whole shoot．For cultivars ‘Daniel cushion’and ‘Tamaryuu’．the multiplication coeficients reached 18 and 8 respectively． On the half strength MS medium supplemented with IBA 0．2 mg/L．the rate of rooting of in vitro shoots of P.subulata L． reached 80％.The survival rate of
  plants transplanted on a large scale was more than 90％ ．The eficiency of multiplication of Phlox subulata L．was afected significantly by genotypes．
• Related Articles | Metrics

• Tissue Culture and Rapid Propagation of Veronicastrum sibricum Pennel
• Jiang Hongbo;Jia Guixia;Ding Qiong
• Acta Horticulturae Sinica. 2004, 31(3): 400-402.
• Abstract ( 1680 ) HTML ( 1069 ) PDF (247KB) ( 1069 ) 　　　
• The explants from seed segments of Veronicastrum sibiricum (L．)Pennell were used to establish culture in vitro and regeneration system．The experiments showed that the best medium for callus induction from seed segm ents was MS with 3 mg·L^-1 6-BA and 0．3 mg·L^-1 NAA．Th e medium supplemented with 2 mg·L^-16-BA and 0.2 mg·L^-1 NAA was favorable to the diferentiation and proliferation of buds, and each explant regenerated 12-18 plantlets about 60 days．The growth of roots was best on 1/2 MS medium with supplement of IBA 0．5 mg·L^-1．
• Related Articles | Metrics

综述

• Classical and Molecular Genetics of Bacterial Wilt Resistance in Tomato
• Wang Guoping;Lin Mingbao;Wu Dinghua
• Acta Horticulturae Sinica. 2004, 31(3): 403-407.
• Abstract ( 1537 ) HTML ( 1829 ) PDF (241KB) ( 1829 ) 　　　
• Studies on inheritance of resistance to bacterial wilt caused by Ralstonia solanaceanum in tomato were reviewed under the following headings：1)Germplasm sources of resistance；2)Classical genetics of resistance；3)Molecular genetics of resistance；4)Discussions．
• Related Articles | Metrics

• Application of Plant Growth Regulator on Ornamental Plant
• Chu Aixiang;Kong Xiangsheng;Zhang Yaozhan
• Acta Horticulturae Sinica. 2004, 31(3): 408-412.
• Abstract ( 1412 ) HTML ( 2821 ) PDF (246KB) ( 2821 ) 　　　
• The effect of application，the mechanism of action，the issue and notice proceeding in application course about plant growth regulator on ornamental plant is summarized．That plant growth regulator apply on ornamental plant can accelerate rootage of quickset，control plant shape，regulation florescence，increase stress-resistance; and can relieve dormancy，enhance the number of tiller and corm，improve the rate of reproduction．
• Related Articles | Metrics

新技术与新方法

• Mericlone Production Technical of Phalaenopsis
• Li Jun;Chai Xianghua;Zeng Baodang;Zhang Xiushan;Wang Xiyan;Zhan Haiyang;Zhu Baoqin
• Acta Horticulturae Sinica. 2004, 31(3): 413-414.
• Abstract ( 1445 ) HTML ( 2798 ) PDF (85KB) ( 2798 ) 　　　
• This paper dealt with rapid propagation and factorial production of Phalaenopsis.
• Related Articles | Metrics

新品种

• Early-ripening Seedless Grape ‘Jingzaojing’
• Fan Peige;Yang Meirong;Zhang Yingzhu;Li Shengchen
• Acta Horticulturae Sinica. 2004, 31(3): 415-415.
• Abstract ( 2087 ) HTML ( 1079 ) PDF (51KB) ( 1079 ) 　　　
• ‘Jingzaojing’is a hybrid euhivar derived from ‘Queen of Vineyard’בThompson Seedless’．The ripening time of berry is in the end of July in Beijing．The fruit cluster is conical，weighing 427.6 g on average． The average mass of a single berry is 2.5-3 g. The berry is egg-like，greenish-yellow and seedless with crisp flesh，juiey and a bit of Muscat aroma when fully ripened，with 16．4％ 一20．3％ soluble solid and 0.47%-0.62% titratable acid．It has medium productivity and resistance to diseases．
• Related Articles | Metrics

• A New Early-ripening Peach Variety ‘Chunxue’
• Li Linguang;Yang Jianming;Sun Yugang;Sui Shuguang
• Acta Horticulturae Sinica. 2004, 31(3): 416-416.
• Abstract ( 1547 ) HTML ( 933 ) PDF (45KB) ( 933 ) 　　　
• ‘Chunxue’，a new peach variety was selected from the germplasm resources of peach introduced from abroad．The fruit shape is round and the average weight per fruit is 150 g. Fruit ripes in medilate June，its skin is red and flesh is crisp．This variety is early-bearing，with a high and constant yield．
• Related Articles | Metrics

• ‘Yan An 1’，A New Pear Variety
• Qi Yongshun;Liu Jin;Song Jinyao;Gao Haisheng;Dong Cuntian;Xiang Dianfang
• Acta Horticulturae Sinica. 2004, 31(3): 417-417.
• Abstract ( 1644 ) HTML ( 945 ) PDF (47KB) ( 945 ) 　　　
• Yan An 1’ is a new pear variety，selected from the colony of Pyrus ussuriensis Maxim. in Yanshan．The soluble solids content is 16.4％ ，the titratable acid content is 1.02％ ，and the vitamin C content is 10.52 mg·kg^-1, the culture has all average fruit mass of 109.4 g and matured in late September to early October in Qinhuangdao．Its flesh is fine and tender in texture with the aroma and flavor of Anli．
• Related Articles | Metrics

• ‘Yanliang Xiangzao’ --A New Drying Variety with Anti-crack of Zizyphus jujuba Mill
• Li Xin gang;Huang Jian;Gao Wenhai;Zhang Kangzhan;Tong Jinxia
• Acta Horticulturae Sinica. 2004, 31(3): 418-418.
• Abstract ( 1619 ) HTML ( 1044 ) PDF (49KB) ( 1044 ) 　　　
• ‘Yanliang Xiangzao’，a new dried-jujube variety with late ripening，selected from Lintong Chizao jujube orchards in Yanliang District，Xi’an．The fruit is oblate shape，red-black colour，and middle large，the average fruit mass is 9.5 g，maximum fruit mass is 15.0 g，the average core mass is 0.4 g.It ripens
  in early-middle October in Yanliang．Th e fruit is uniform，attractive，good quality fruit，applicable to dry，and it has 46％ of the dried-rate．The fresh-fruit has a middle-thick peel with high toughness and crack-resistance． The dried-jujube with plump-fruit has a good elasticity and highlight color．Its cultivated region is in north China with 11.0-13.0℃ of the annual average temperature and 550-650 mm of the annual rainfall．
• Related Articles | Metrics

• A New Cultivar of Actinidia deliciosa ‘Zhongmi 1'
• Han Lixing;Huang Zhenguang;Li Ming;Zhao Gairong;Li Yuhong;Pang Fengqi;Pang Yu
• Acta Horticulturae Sinica. 2004, 31(3): 419-419.
• Abstract ( 1796 ) HTML ( 1014 ) PDF (54KB) ( 1014 ) 　　　
• ‘Zhongmi 1’ is a new cuhivar of Actinidia deliciosa selected from the wild in Funiu mountain area，He’nan province.It has the characteristics of large fruit size with small core and less seeds，green flesh.delicious flavour and fine quality，early fruiting and high yield，vigorous growth. ‘Zhongrni 1’ has a high ability of the resistance to the diferent stresses such as drought，cold and grow good in mountain area with poor soil．Harvested in October, ‘Zhongmi 1’ is a superior cuhivar for the flesh fruit market．
• Related Articles | Metrics

• Development of New Chinese Cabbage Varietie
• Sun Rifei;Zhang Shujang;Li Fei;Zhang Shifan;Niu Xinke
• Acta Horticulturae Sinica. 2004, 31(3): 420-420.
• Abstract ( 1394 ) HTML ( 1066 ) PDF (48KB) ( 1066 ) 　　　
• Chinese cabbage originated in China．Genetic resourse enhancement and breeding of Chinese cabbage have been conducted for more than 30 years in this institute．Self-incompatility，male sterility，isolated microspore culture and molecular marker assistant selection were applied to breed several new varieties: Zhongbai 80，Zhongbai 78，Zhongbai 66 and Zhongbai 50．
• Related Articles | Metrics

• A New High-quality Non-heading Chinese Cabbage Variety ‘Shu Lü’
• Hou Xilin;Cao Shouchun;Zhang Shuning;Zhang Zengcui;Wang Jianjun;Sun Hongxia
• Acta Horticulturae Sinica. 2004, 31(3): 421-421.
• Abstract ( 1664 ) HTML ( 1086 ) PDF (50KB) ( 1086 ) 　　　
• ‘Shu Lü’ is a hybrid developed by crossing two non-heading Chinese cabbage SW-13 and SU-124(Ai×Su)characterized by upstanding plant figure，bunchy waist，nearly round and light green leaf, half-round and white-green petiole． It’s with high quality and beautiful figure. The mass ratio of blade to petiole is 0.67. The average mass of single plant is about 0.40 kg. It is highly resistant to anthracnose,and resistance to TuMV．downy mildew and ahernaria leaf spot with heat．resistance．It is suitable for growing in summer，autumn and winter in the regions where green-stem cabbage is popular．
  
• Related Articles | Metrics

• ‘Jiyan 8’- A Pepper Hybrid Produced by Male Sterile Lines
• Fan Yanqin;Liu Yun;Yan Libin
• Acta Horticulturae Sinica. 2004, 31(3): 422-422.
• Abstract ( 1405 ) HTML ( 939 ) PDF (49KB) ( 939 ) 　　　
• ‘Jiyan 8’is a hybrid pepper by crossing the genic male sterile line AB91-8 with an ox-horn pepper inbred line L16-2.The variety has early-middle maturity and good tolerance to diseases． Fruit is the ox-horn shape. It has high yield and good quality．The variety is suitable for protected and open field production．
  
• Related Articles | Metrics

• A New Cherry Tomato Hybrid ‘Small Tomato Jinong 2’
• Yao Fangjie;Su Guijuan;Ye Jingxue
• Acta Horticulturae Sinica. 2004, 31(3): 423-423.
• Abstract ( 1555 ) HTML ( 1086 ) PDF (40KB) ( 1086 ) 　　　
• ‘Small tomato Jinong 2’ is a F₁ by crossing the variety red cherry with small tomato introduced from Denmark．It has unlimited branches and common lear and it grows vigorously．The first inflorescence is situated between the eighth and ninth node and each inflorescence bears 20-40 tomatos．The fruit is red and round and it is very tasty．Each weighs 10-15 g. It is resistant to disease and is suitable for open field cultivation and greenhouse cultivation．
  
• Related Articles | Metrics