Research Papers

• Gene Cloning and Functional Identification of an Apple MdMYB2 Gene
• QU Fengjia，AN Jianping，YAO Jifang，LI Haohao，LI Yuanyuan，YOU Chunxiang，WANG Xiaofei*，and HAO Yujin*
• Acta Horticulturae Sinica. 2017, 44(12): 2245-2254. DOI:10.16420/j.issn.0513-353x.2017-0347
• Abstract ( 504 ) HTML ( 1065 ) PDF (2018KB) ( 1065 ) 　　　
• MdMYB2 gene was cloned from Malus × domestica‘Gala’with homologous cloning using RT-PCR method. Its open reading frame（ORF）contained 873 bp nucleotides，which encodes a predicted protein containing 290 amino acid residues. The predicted protein has a molecular mass of 32.92 kD and a pI value of 4.91. Phylogenetic analysis demonstrated that MdMYB2 showed the highest evolutionary relationship with PbMYBL2 from pear. Expression analysis showed that the MdMYB2 gene was constitutively expressed in different tissues，with rather higher expression levels in fruit and stem than in others. Meanwhile，its expression was noticeably induced by salt stress treatment. Finally，transgenic apple calli and Arabidopsis were obtained with Agrobacterium-mediated genetic transformation. Tolerance assays indicated that overexpression of MdMYB2 remarkably enhanced the tolerance of transgenic apple calli to high salinity，further supporting that MdMYB2 positively regulates salt tolerance in apple. In addition，ectopic expression of MdMYB2 also enhanced salt stress tolerance in Arabidopsis，indicating that MdMYB2 plays an important role in plant salt stress tolerance.
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• Functional Identification of Salt Tolerance and ABA Sensitivity of Apple U-box E3 Ubiquitin Ligase MdPUB24
• QI Chenhui，ZHAO Xianyan，HAN Pengliang，JIANG Han，WANG Yongxu，HU Dagang*，and HAO Yujin*
• Acta Horticulturae Sinica. 2017, 44(12): 2255-2264. DOI:10.16420/j.issn.0513-353x.2017-0430
• Abstract ( 269 ) HTML ( 920 ) PDF (1890KB) ( 920 ) 　　　
• An E3 ubiquitin ligase gene（Gene ID：MDP0000199588）was cloned from‘Royal Gala’ apple（Malus × domestica Borkh.）. Sequence analysis showed that the length of MdPUB24 gene was 1 212 bp，which encoded 404 amino acids. A phylogenetic tree analysis indicated that our cloned apple E3 ubiquitin ligase exhibited the highest sequence similarity to Arabidopsis AtPUB24，so named MdPUB24. In silico analysis suggested that the promoter sequence of MdPUB24 contained several typical cis-acting elements，including abscisic acid-，light-，MeJA- and drought-responsive elements using PlantCare Databases. qPCR analysis showed that MdPUB24 widely expressed in different tissues of‘Royal Gala’ apple，and highly expressed in apple leaves. The expression of MdPUB24 was significantly inhibited by exogenous ABA and NaCl and low temperature. The growing potential of MdPUB24-expressing apple calli of‘Orin’cultivar and Arabidopsis were much weaker than that of control under salt stress condition，suggesting that MdPUB24 negatively regulated salt tolerance. In contrast，the growing potential of MdPUB24-expressing apple calli of‘Orin’and Arabidopsis were much stronger than that of control in the presence of exogenous ABA，suggesting that MdPUB24 was insensitive to exogenous ABA.
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• Cloning and Expression Analysis of HLB-associated Salicylic Acid Carboxyl Methyltransferase Gene CsSAMT-1 in Citrus
• BAI Xiaojing，XU Lanzhen，JIA Ruirui，ZHOU Pengfei，CHEN Min，HE Yongrui，PENG Aihong，LEI Tiangang，LI Qiang，YAO LiXiao，CHEN Shanchun*，and ZOU Xiuping*
• Acta Horticulturae Sinica. 2017, 44(12): 2265-2274. DOI:10.16420/j.issn.0513-353x.2017-0320
• Abstract ( 306 ) HTML ( 1019 ) PDF (1643KB) ( 1019 ) 　　　
• Salicylic acid（SA）plays a pivotal role in plant resistance to pathogens. Salicylic acid carboxyl methyltransferase（SAMT）is an enzyme for catalyzing the formation of methyl salicylate（MeSA）from SA. Here，we cloned a SAMT gene named as CsSAMT-1，which was associated with Huanglongbing（HLB）infection. In HLB-susceptible Jincheng orange（Citrus sinensis Osbeck），the expression level of CsSAMT-1 was highly induced by HLB infection，in contrast to the constitutive expression of CsSAMT-1 in a HLB-resistant variety，sour pomelo（C. grandis Osbeck）. CsSAMT-1 was also induced by SA and methyl jasmonate（MeJA）in both Jincheng orange and sour pomelo. Moreover，the expression level of CsSAMT-1 in the vein was higher than that in the mesophyll. The contents of SA and MeSA in the vein of sour pomelo were 11.85 and 18.18 ng ? g-1，respectively，which were significantly higher than that（0.82 and 0.0072 ng ? g-1）in the vein of Jingcheng orange. After HLB infection，the contents of both SA and MeSA were markedly increased in Jincheng orange；while in sour pomelo，SA content was decreased and MeSA remained unchanged. Taken together，our results suggest that CsSAMT-1 may confer resistance to HLB through mediating the conversion of SA and MeSA in citrus.
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• Analysis on Evolutionary Characteristics and the Temporal and Spatial Expression Patterns of miR166 Gene Family in Dimocarpus longan
• LIN Yuling，ZHANG Qinglin，ZENG Youjing，CHEN Xiaohui，ZHANG Zihao，CHEN Yukun，and LAI Zhongxiong*
• Acta Horticulturae Sinica. 2017, 44(12): 2285-2295. DOI:10.16420/j.issn.0513-353x.2017-0073
• Abstract ( 285 ) HTML ( 940 ) PDF (2971KB) ( 940 ) 　　　

• In order to understand the evolution characteristics of miR166 gene family and its expression pattern in different tissues of Dimocarpus longan，we analyzed the mature sequences，and the phylogenetic of miR166 in longan and some other fruit plants，predicted the secondary structure of precursor miR166（pre-miR166）family members，analyzed their targets，as well as their expression patterns. The results showed that the longan miR166 family contained 12 mature sequences and 7 precursor sequences. Mfold analysis showed that these pre-miR166 members could form a typical stable stem loop structures，and their minimal folding free energies（ΔG）ranged from–35.30 to–83.30 kal ? mol-1. Multiple sequence alignment of these pre-miR166 members showed that the regions generating mature miRNA were highly conservative. Phylogenetic tree analysis showed that the genetic relationship of pre-miR166 family in longan was closer to those from grape and peach. Target gene prediction of longan miR166 family revealed that their targets were homeobox-leucine zipper protein，pentatricopeptide repeat-containing protein，hypothetical protein，DNA-directed RNA polymerase Ⅲ subunit，and Ultraviolet-B receptor UVR8. Real-time quantitative PCR showed that diffierent pre-miR166 family members，had different expression patterns，and more or less expressed at different development stages on vegetative and reproductive growth of longan，suggesting that they may be widely participate in the regulation of longan organs development，and may have function division in longan.

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• MrMYB1-MrbHLH1，a Potential Visible Reporter Gene in Horticultural Plant Transformation
• ZHANG Xili，YIN Xueren，LI Fang，CHEN Kunsong，and LIU Xiaofen*
• Acta Horticulturae Sinica. 2017, 44(12): 2296-2304. DOI:10.16420/j.issn.0513-353x.2017-0432
• Abstract ( 400 ) HTML ( 841 ) PDF (4626KB) ( 841 ) 　　　
• The binary vector，contained both MrMYB1 and MrbHLH1，which were related to anthocyanin biosynthesis regulation in Chinese bayberry was recombined. Subsequently，it was transformed into tobacco，tomato and strawberry，respectively. The 35S::MrMYB1-MrbHLH1 lines of these different species were successfully obtained through Agrobacterium-mediated transformation，with accumulation of anthocyanin and red buds，while the wild type plants have the green buds. Furthermore，anthocyanin accumulations were also observed in various tissues of the transgenic tobacco and tomato plants，which are hereditable. Taken together，MrMYB1-MrbHLH1 has the conserved function for anthocyanin biosynthesis in three kinds of horticultural plants and exhibited the potential to be used as a visible reporter gene in plant transformation.
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• Genome-wide Identification and Expression Analysis of GRAS Gene Family in Pepper
• ZHANG Huanxin，DONG Chunjuan，and SHANG Qingmao*
• Acta Horticulturae Sinica. 2017, 44(12): 2305-2317. DOI:10.16420/j.issn.0513-353x.2017-0132
• Abstract ( 455 ) HTML ( 952 ) PDF (1741KB) ( 952 ) 　　　
• In this study，we systematically investigated the member of CaGRAS gene family in pepper（Capsicum annuum L.）and elucidated their phylogenetic relationships，tissue-specific transcription patterns，and responses to PEG6000 and salt stresses. A total of 54 CaGRAS genes were identified and they distributed on all chromosomes except the 11th. The number of exons ranged from 1 to 3，and isoelectric points ranged from 4.97 to 9.13. Phylogenetic analysis showed that CaGRAS genes were clustered into eight phylogenetic groups. The CaGRAS genes exhibited different expression patterns in distinct pepper tissues. Among them，34 genes highly expressed in roots，eight genes in stems，three genes in mature leaves and eight genes in shoot tips. Moreover，most CaGRAS genes were responsive to PEG6000 and salt stresses. Some genes including CaGRAS11，CaGRAS30，CaGRAS40，CaGRAS44 and CaGRAS50 were greatly induced by both PEG6000 and salt stresses. These results will provide a foundation for further functional research on CaGRAS genes in pepper.
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• Studies of Indexes and Evaluation of Low Nitrogen Tolerance of Pepper at Seedling Stage
• WANG Chunping1,*，ZHANG Shicai2,*，LEI Kairong1，HUANG Renzhong2，Lü Zhonghua2，WU Hong1，JIANG Xiaoying1，LI Yifei2，TANG Rongli1，HUANG Qizhong2,**，and LIN Qing1,**
• Acta Horticulturae Sinica. 2017, 44(12): 2318-2326. DOI:10.16420/j.issn.0513-353x.2017-0255
• Abstract ( 273 ) HTML ( 691 ) PDF (734KB) ( 691 ) 　　　
• To develop a rapid and effective method for screening low nitrogen resistant pepper cultivars at seedling stage，the tolerance of 25 different pepper genotypes under low nitrogen stress was evaluated by hydroponic investigations. It indicated that 18 morphological and physiological indexes of pepper at seedling stage were affected by low nitrogen treatment，however，the effect degrees among genotypes were significantly different. Based on their higher coefficients of variation，relative values of 8 indexes including leaf area，dry weight of shoot，dry weight of root，root-shoot ratio of dry weight，dry weight of plant，nitrogen accumulation of shoot，nitrogen accumulation of root and nitrogen accumulation of plant were selected as the key indexes for screening and evaluation of low nitrogen tolerance of pepper. The low nitrogen tolerance of pepper was evaluated by fuzzy membership function with the normalized coefficients of variation of the 8 key indexes and all of the 18 indexes as weight，and the evaluation results were compared with the results of principal component analysis. It was showed that the evaluation results of the two analysis method were largely identical，so were the 8 key indexes and the 18 indexes，the coincidences rates of low nitrogen tolerance and sensitivity of the first 5 materials were above 80%. The nitrogen tolerance of 3 pepper materials with different degree of nitrogen tolerance at seedling stage was evaluated at maturity stage and it was revealed that the results were largely identical to that of seedling stage. Excepted for root-shoot ration of dry weight，significant correlations between the other 7 of the 8 key screening indexes and the nitrogen tolerance of pepper at maturity stage were observed. It is suggested that we can effectively and in large quantities evaluated the nitrogen tolerance of pepper at seedling stage by the 8 key screening indexes and fuzzy membership function.
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• Phytophthora Disease Control and Growth Promotion of Pepper by Pythium oligandrum
• YIN Jie and YUAN Ling*
• Acta Horticulturae Sinica. 2017, 44(12): 2327-2337. DOI:10.16420/j.issn.0513-353x.2017-0273
• Abstract ( 485 ) HTML ( 748 ) PDF (1221KB) ( 748 ) 　　　
• In order to use Pythium oligandrum，develop safe，nontoxic and high-efficient biological agent and control Phytophthora disease in pepper cultivation. P. oligandrum CQ2010，a self-isolated biocontrol strain，was used to produce biological agent（P. oligandrum agent，POA）and then the effects of POA on Phytophthora disease control and growth-promotion were investigated through culture，antagonism，and field experiments. P. oligandrum CQ2010 secreted cellulase，protease，β-1,3-glucanase and chitinase. The hydrolysis circle diameters of the former three enzima were about or over than colonies on solid culture media. The extract of POA significantly inhibited the mycelial growth of Phytophthora capsici and the inhibitory rate was higher than metalaxyl Mancozeb and lower than Mandipropamid. In field experiment，the addition of POA into the soil around the roots of pepper seedlings significantly decreased the disease incidence and index of Phytophthora disease. POA showed a better preventive effect than the curative，which were 89.37% and 61.19%，respectively. Pepper plants applied POA bloomed and fruited 6–9 days earlier than the control（without POA）. In addition，POA application increased plant biomass by 32.03%，nutrient absorption by 35.74%（N），40.53%（P）and 53.23%（K），and fruit yield by 25.21%. Concomitantly, fruit quality was improved due to higher soluble proteins, free amino acids, soluble sugars，and vitamin C but similar content of nitrate compared to the control. P. oligandrum CQ2010 effused enzymes that hydrolyze the cell walls of pathogenic fungi. The extract of POA inhibited the mycelial growth of Phytophthora capsici. POA application decreased the disease incidence and index of pepper Phytophthora，promoted plant growth and development，increased fruit yields，and improved fruit quality. Therefore，POA has a broad application prospect and will be suitable for mass production.
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• Analysis of Green-center Characteristics and Establishment of Green-center Comprehensive Evaluation Standard for 49 Spray Cut Chrysanthemum Cultivars
• ZHAO Qianru，FANG Weimin，GUAN Zhiyong，CHEN Fadi，and TENG Nianjun*
• Acta Horticulturae Sinica. 2017, 44(12): 2338-2350. DOI:10.16420/j.issn.0513-353x.2017-0403
• Abstract ( 469 ) HTML ( 672 ) PDF (2579KB) ( 672 ) 　　　
• In this study，green-center characteristics of spray cut chrysanthemum and the key factors influencing green-center quality were systematically investigated for the purpose of establishing foundation for the breeding of green-center spray cut chrysanthemum. It was found that L*，h，C* of inflorescence center color at the beginning of full flower stage were 37.18–54.05，93.69°–114.76° and 42.84–64.09，respectively. The relative area of green center at the beginning of full flower stage was 13.05%–100.00%，and the duration of green center was 0–15 days. In addition，the weights of five parameters（i.e. the inflorescence center color at the beginning of full flower stage，the relative area of green center at the beginning of full flower stage，the duration of green center，the yellowing time of inflorescence center，and the ornamental value of green center at the end of full flower stage）were 0.273，0.237，0.252，0.136，and 0.101，respectively，by comprehensive scoring method and principal component analysis. Furthermore，49 cultivars were divided into 4 groups of excellent，good，medium and poor，and the numbers of cultivars in each group were 10，12，16 and 11，respectively by grading and scoring of each index，weight calculation and cluster analysis of weighted total scores. Taken together，these results suggest that green-center characteristics are mainly determined by the inflorescence center color at the beginning of full flower stage，the relative area of green center at the beginning of full flower stage，and the duration of green center. The green-center comprehensive evaluation standard will be useful for evaluation and development of green-center spray cut chrysanthemum.
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Research Notes

• The Growth and Gene Expression Characteristics of Fragaria × ananassa ‘Ningyu’Response to Drought Stress
• ZHANG Chaobo，LI Ao，CUI Mengjie，LIU Zhongjie，CHEN Lide，JIA Haifeng，and FANG Jinggui*
• Acta Horticulturae Sinica. 2017, 44(12): 2351-2360. DOI:10.16420/j.issn.0513-353x.2016-0497
• Abstract ( 304 ) HTML ( 875 ) PDF (1284KB) ( 875 ) 　　　
• In order to understand the feasibility of genetic diagnosis for strawberry under drought stress，it is necessary to recognize the growth and development characteristics of strawberry under drought conditions and information for the expression of important genes and their relationships. Fragaria × ananassa‘Ningyu’was used as material to analyze the growth characteristics and 10 genes expression related to auxin synthesis，abscisic acid synthesis，flower and berry development，pigment synthesis under different drought stress. The results showed that strawberry can complete the life cycle from vegetative growth to reproductive growth under different drought conditions；the selected genes are also exercise and completed its regulation function. Comparing with the control plants，the phenology phases of strawberry under mild or moderate drought conditions with 50%–65% of soil water holding capacity were earlier than in control（80% soil water holding capacity），when severe stress that the soil water holding capacity of 35%，the growth and development of strawberry were delayed，with the increasing of drought stress，strawberry plants，crown diameter，leaves，and berries were become significantly smaller than the control，but with deep color；under different drought conditions，the trend of growth-related gene expression in strawberry were similar basically，the time of gene expression was in accordance with the phase of phenology and the growth length of the corresponding plants under all kinds of drought stress. In mild to moderate drought conditions，the expression time of these genes advanced，with a shorter expression period，but gene expression level increased comparing with the control；while in severe drought conditions，the beginning time of gene expression was close to the control，but the expression period elongated，with low level of gene expression. It can be deduced from gene expression that the mechanism of smaller plant，fewer and smaller berry under light and moderate drought stress might not be the same as that under severe drought stress；the shorter gene expression period and lower expression might be one of the reason which causing the smaller crown diameter，smaller leaves and berries or fewer berries.
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• Variations of Anthocyanin and Chlorophyll Contents and Composition in ‘Northland’Blueberry Leaf Throughout the Color Changing Process in Autumn
• LI Binbin，YANG Junfeng，GAO Yuan，PAN Meina，and HOU Zhixia*
• Acta Horticulturae Sinica. 2017, 44(12): 2361-2371. DOI:10.16420/j.issn.0513-353x.2017-0116
• Abstract ( 220 ) HTML ( 826 ) PDF (939KB) ( 826 ) 　　　
• Change characterizations of the contents and compositions of the anthocyanin and the chlorophyll were studied in 4-year-old‘Northland’blueberry as materials during leaf color transformation in autumn. With the change of leaf color，the total amount of anthocyanin increased，and the greater the increase in the later stages. However，the total amount of chlorophyll decreased with larger decline in the initial stage. Five kinds of anthocyanins were detected in the full green leaf. Along with the leaf color transformation，more anthocyanidins were detected. Eleven kinds of anthocyanins，which belong to 4 kinds of cyanidins：delphinidin，petunidin，malvinidin and peonidin，were detected in the red leaves. Among all the anthocyanins，delphinindin-3-O-arabinoside，petunidin-3-O-galactose and petunidin-3-O-glucose were three kinds of main anthocyanins，which co-led the trend of total anthocyanins. Intrestingly，along with the transformation of leaf color，the total amounts of the delphinidin and petunidin increased，while their ratio remained unchanged. The trend of the Chl.a and Chl.b contents were consistent，in which the Chl.a had higher content and larger change range than Chl.b. The comprehensive analysis showed that the increase of anthocyanin accumulation and the decrease of chlorophyll accumulation were the key factors of leaf color transformation. The delphinidin and petunidin were the main ancyanidins，and their contents and ratio might determined the“red”color of leaf. During leaf color transformation，three kinds of anthocyanidins were mainly accumulated：delphinidin-3-O-arabinoside，petunidin-3-O-galacoside and petunidin-3-O- glucoside，affecting the trend of total amount of anthocyanins，the main pigment ratios and the leaf color，furtherly.
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• Effects of Red and Blue Light Qualities on Main Fruit Quality of Tomato During Color-turning Period
• LI Yan1,2,3,4,*，WANG Liwei5，WEN Lianlian1，WEI Min1,2,4，SHI Qinghua1,2,3,4，YANG Fengjuan1,3,4，and WANG Xiufeng1,3
• Acta Horticulturae Sinica. 2017, 44(12): 2372-2382. DOI:10.16420/j.issn.0513-353x.2017-0171
• Abstract ( 357 ) HTML ( 952 ) PDF (4080KB) ( 952 ) 　　　

• The LED as precision modulation light sources were used with 3 treatments including red（R）light，blue（B）light and combination of red and blue light with proportion of 3︰1（3R1B），white light as control. The effects of red and blue light qualities on main fruit quality and gene expression of key enzymes involved in the biosynthesis of lycopene of tomato with‘Micro-Tom’as experiment material during color-turning period were studied. The results showed that compared with control，red and 3R1B light treatment could significantly increase the soluble sugar content and ratio of sugar and acid in tomato fruit，but reduced the titratable acid content. The content of vitamin C，soluble protein and titratable acid under blue light was significantly enhanced，while lower in the soluble sugar content and no significant difference was found compared with control. With the fruit maturation，the lycopene content and gene expression of geranylgeranyl pyrophosphate synthase（GGPS）and phytoene synthase（PSY）were significantly higher under red and 3R1B light than those under blue and white light，especially for 3R1B. Furthermore，the lycopene content was significantly reduced and the expression of GGPS and PSY1 was inhibited by blue light. In summary，blue light could promote the increment in levels of vitamin C，protein and organic acids. Red and combination of red and blue light were more conducive to the accumulation of carbohydrates，and promoted lycopene synthesis by enhancing the transcription levels and activities of GGPS and PSY1，which involved in the biosynthesis of lycopene，especially the latter treatment. It indicated that the combination of red and blue light with appropriate proportion could promote the color-turning of tomato fruit and improve fruit quality.

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• Technical System Construction of Plasmodiophora brassicae Single-spore Isolation and Identification of Plasmodiophora brassicae in Shenyang Area
• LIU Yifan，WANG Sai，CHANG Hong，GAO Shiqi，ZHAO Ying，JI Ruiqin*，and FENG Hui
• Acta Horticulturae Sinica. 2017, 44(12): 2383-2390. DOI:10.16420/j.issn.0513-353x.2017-0497
• Abstract ( 271 ) HTML ( 674 ) PDF (1815KB) ( 674 ) 　　　

• 芸薹根肿菌严重危害大白菜和其他十字花科作物。由于根肿菌通常以多个生理小种混生状态进行侵染，导致抗病品种的抗性不能持久。以沈阳地区根肿菌为材料，进行了单孢分离技术体系构建，结果表明采取冷冻盒接菌法对两日龄大白菜幼苗进行单孢接种，可将侵染率提高到47.86%；采用基质过渡培养可将大白菜植株成活率提高到67%。利用上述根肿菌单孢分离技术体系对沈阳地区根肿菌进行单孢分离和Williams生理小种鉴定，结果表明沈阳地区根肿菌存在4号、8号、9号、11号生理小种，其中4号生理小种为优势小种。

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• Study on the Flower Bud Metabolomics of Petunia hybrida‘Fantasy’Grown Under Weak Light or Short Day Conditions
• YUE Yuanzheng1,2，LIU Siyu2，YIN Chaoqun2，GUO Rui2，YANG Mei2，TIAN Shaoze2，and HU Huirong2,*
• Acta Horticulturae Sinica. 2017, 44(12): 2391-2398. DOI:10.16420/j.issn.0513-353x.2016-0496
• Abstract ( 263 ) HTML ( 867 ) PDF (725KB) ( 867 ) 　　　

• The flower bud number and metabolism of Petunia hybrida‘Fantasy’under the condition of control（light strength 11.5 μmol ? m-2 ? s-1，day length 16 h），weak light（3.6 μmol ? m-2 ? s-1，16 h）and short day（11.5 μmol ? m-2 ? s-1，8 h）were observed and recorded. Observation on flower buds showed that the number of normal and total buds decreased dramatically while aborted buds increased significantly under either the weak light or short day stress. Metabolites were identified by mass spectra retrieval and spectra resolution combined with retention index. Then，we went on multivariate statistical analysis and determined semi-quantitatively by peak area normalization method. The results were as follows：we found out 111 peaks and 42 of them were identified. There was significant difference in metabolism of flower bud of Petunia hybrida‘Fantasy’under the condition of control，weak light and short day. Compared with control，the metabolic shifts was shown as the increasing of various amino acids and the decreasing of various sugars. And weak light had larger influence on metabolism of the flower bud than that of short day. Depletion of proteins and sugars was reckoned to be one of the reasons that lead to aborted flower bud under the weak light or short day stress.

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• Research on Construction of Sense and Antisense Plant Expression Vectors of NtPLATZ1 Gene in Narcissus tazetta var. chinensis and Genetic Transformation to Tobacco Plants
• WANG Weiying，LI Haiming，DAI Yimin，and LIN Jiangbo*
• Acta Horticulturae Sinica. 2017, 44(12): 2399-2407. DOI:10.16420/j.issn.0513-353x.2017-0188
• Abstract ( 259 ) HTML ( 657 ) PDF (2005KB) ( 657 ) 　　　

• To characterize the function of NtPLATZ1 gene from Narcissus tazetta var. chinensis，sense and antisense plant expression vectors were generated and transferred into Agrobacterium tumefaciens strain EHA105. Then vectors were transformed into tobacco plants by using Agrobacterium-mediated method. Regenerated plants of tobacco were detected by PCR-Southern blot and RT-PCR. The results showed that the sense and antisense genes were successfully integrated into the genomes of tobacco cells and expressed in transgenic tobacco plantlets. Transgenic plants were obtained successfully，which were inhibited the growth when the gene was over expression，and were promoted the growth when the gene was inhibited expression.

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Review

• Advances in the Research on Salt Tolerance of Potato
• LI Qing1,2，QIN Yuzhi2，HU Xinxi2，WANG Wanxing1,*，and XIONG Xingyao1,2,*
• Acta Horticulturae Sinica. 2017, 44(12): 2408-2424. DOI:10.16420/j.issn.0513-353x.2017-0472
• Abstract ( 358 ) HTML ( 1030 ) PDF (1156KB) ( 1030 ) 　　　
• The present status of salt tolerance in potato was reviewed from 4 aspects：the evaluation of salt tolerance resources in potato，physiological response and molecular mechanism under salt stress and potato improvement with salt tolerance by genetic engineering. Meanwhile，the related problems of potato salt tolerance research are discussed and prospected. Some suggestions are as references to reveal the mechanism of salt tolerance in potato，identify salt resistant resources and innovate salt tolerant varieties.
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New Cultivars

• ‘Zijinhong’，a New Red-flowered Strawberry Cultivar
• WANG Qinglian，ZHAO Mizhen*，WANG Zhuangwei，WU Weimin，and QIAN Yaming
• Acta Horticulturae Sinica. 2017, 44(12): 2425-2426. DOI:10.16420/j.issn.0513-353x.2017-0211
• Abstract ( 914 ) HTML ( 602 ) PDF (1117KB) ( 602 ) 　　　
• A new ornamental and edible red-flowered strawberry cultivar，‘Zijinhong’was released from the cross between‘Benihoppe’as female parent and‘03-01’（derived from‘Pink Panda’and‘Shuoxiang’）as male parent. It had pink flower with overlapping petals. Its fruit was red，conical and uniform with average single primary fruit weight 18.5 g. It tasted sweet and the content of soluble solids was 8.0%–13.7%. The fruit firmness was much better with 1.95 kg ? cm-2. It showed moderately resistant to anthracnose and stronger ability of stolon. It was suitable for fresh food and ornamental.
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• A New Pepper F1 Hybrid‘Longjiao 10’
• WANG Lanlan*，CHEN Lingzhi，WEI Bingqiang，and ZHANG Ru
• Acta Horticulturae Sinica. 2017, 44(12): 2427-2428. DOI:10.16420/j.issn.0513-353x.2017-0178
• Abstract ( 429 ) HTML ( 937 ) PDF (1337KB) ( 937 ) 　　　
• A new hot pepper F1 hybrid‘Longjiao 10’was developed by inbred lines‘935’and‘927’ as female and male parent respectively. It’s fruit is horn shaped with wrinkle，28.0 cm in length，3.1 cm in width，0.25 cm in pulp thickness and 62 g in weight. The average yield is 75.0 t ? hm-2. It is resistant to TMV and tolerant to Phytophthora blight. It is suitable for planting in protected and open fields in northwest China.
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• A New Auricularia polytricha Cultivar‘Shener 4’
• YU Hailong1,2,*，Lü Beibei3,*，TAN Qi1，SHANG Xiaodong1，ZHANG Meiyan1，SONG Chunyan1，and ZHANG Lujun1,**
• Acta Horticulturae Sinica. 2017, 44(12): 2429-2430. DOI:10.16420/j.issn.0513-353x.2017-0323
• Abstract ( 315 ) HTML ( 631 ) PDF (794KB) ( 631 ) 　　　
• ‘Shener 4’was bred by system selection from Auricularia polytricha‘Huanger 10’. The cultivar is characterized by about 35 d mycelial culture time，high temperature resistance，good consistency，moderate size fruiting-body，up to 95% biological conversion rate，suitable for drying or fresh sale. The surface of fruiting body is smooth and dark red while the back is dark brown，the dried product with dark brown front and gray white back.
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• A New Morchella Cultivar‘Chuan Yangdujun 6’
• CHEN Ying，TANG Jie，PENG Weihong*，GAN Bingcheng，HUANG Zhongqian，and WANG Yong
• Acta Horticulturae Sinica. 2017, 44(12): 2431-2430. DOI:10.16420/j.issn.0513-353x.2017-0023
• Abstract ( 287 ) HTML ( 779 ) PDF (1132KB) ( 779 ) 　　　
• A new Morchella sextelata cultivar‘Chuan Yangdujun 6’was bred from wild strain which from Kangding County in the Ganzi Tibetan Autonomous Prefecture of Sichuan Province. The fresh fruiting body grow both solitary and caespitose. The color of fresh fruiting body is reddish brown to dark reddish brown. The cap is cuspidal. The density of cap ridges are medium，the vertical ridges are conspicuous. The stipe is glossy and white. Sichuan area and the climate similar area are suitable cultivated place. The growth period is 80–105 d in Chengdu plain region. The yield of fresh fruiting body is 2.775 t ? hm-2.
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