研究论文

• Regeneration and Transformation in Vitro of the Strawberry Varieties
• Zhang Zhihong;Wu Luping;Dai Hongyan;Wang Guoying;Zhao Tianyong;Bi Xiaoying;and Du Guodong
• Acta Horticulturae Sinica. 2001, 28(3): 189-193.
• Abstract ( 1312 ) HTML ( 1749 ) PDF (296KB) ( 1749 ) 　　　
• An efficiency and stable regeneration system in vitro and genetic transformation system for the commercial strawberry varieties were developed , and transgenic plants were obtained. Bud regeneration percentage of leaf pieces of strawberry varieties ‘Tudla’and ‘Senga Sengana’reached 100 %. Leaf pieces from in vitrogrown plants‘Tudla’were cocultivated for 3 days with Agrobacterium tumef aciens EHA105. After the cocultivated leaf pieces were incubated on regeneration medium
  containing 40 mg/ L kanamycin for 4 weeks , the transformed buds appeared on the explants. The regeneration percentage of transformation buds of strawberry variety‘Tudla’reached 6. 8 %. The result of histochemical staining of GUS gene showed , of the 10 independent plants transformed with GUS gene , 5 expressed GUS activity strongly. The plants transformed with bar gene were able to differentiation on the medium containing glufosinate 10 mg/ L , and expressed strongly resistance to glufosinate
  on field. The transgenic plants flowered and fruited normally.
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• Apple Haploid Induction by Pollination with High Dose Irradiated ollen
• He Daoyi;Sun Shan;Cui Decai;and Chen Zhenguang
• Acta Horticulturae Sinica. 2001, 28(3): 194-199.
• Abstract ( 1381 ) HTML ( 1206 ) PDF (247KB) ( 1206 ) 　　　
• The parthenogenic young embryos were gained in apple varieties (Bela , Liaofu and Fuji) due to pollination with radiated pollen by 1 000 - 1 500 Gyγ ray. The haploids of these varieties were produced by young embryo culture and regeneration. The investigation indicated that major factors which limited hapliod production efficiency in apple by such technique included abscission of
  fruits in early stage , young embryo abortion and difficulty in regeneration of young embryo with endosperm. The results also suggested that regeneration of 25 d young embryo (ovule) was available.
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• Studies on the Relationship of the Development of Bitter Pit in Apple Fruits with the Contents of Calcium and Magnesium and the Activities of Antioxidant Enzymes
• Wang Liangju;Jiang Weibing;He Qifeng;and Fan Huangbin
• Acta Horticulturae Sinica. 2001, 28(3): 200-205.
• Abstract ( 1420 ) HTML ( 1562 ) PDF (239KB) ( 1562 ) 　　　
• The calcium contents in all parts of bitter pit apples were significantly lower than that of the sound ones , but only the contents of magnesium at the outer cortex significantly higher than the sound ones. The activities of superoxide dismutase (SOD) , peroxidase ( POX) , catalase (CAT)
  and ascorbate specific peroxidase (APX) in the pitted fruits were significantly lower than that of the sound. Vacuum infiltration of apples with CaCl₂ 100 mmol/ L increased activities of the above antioxi dant enzymes , and the treatment of MgCl₂ decreased the activities of POX, APX and CAT , implying
  the activities of these three enzymes were regulated by calcium. The contents of malonaldehyde and the relative conductivity were higher in bitter pit apple fruits , which showed that the peroxidation of membrane might be the important reason of the physiological disorder in apple with lower level of calcium.
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• Effects of Exogenous Spermidine on the Levels of Endogenous Hormones and Polyamines in the Flowers and Fruitlets of Red Fuji Apple
• Xu Jizhong;Chen Haijiang;Ma Baokun;and Zhang Wencai
• Acta Horticulturae Sinica. 2001, 28(3): 206-210.
• Abstract ( 1539 ) HTML ( 1318 ) PDF (207KB) ( 1318 ) 　　　
• Effects of exogenous polyamines and their biosynthesis inhibitor MGBGon fruit set and the contents of the endogenous hormones and polyamines during flowering and fruit setting were studied with Red Fuji apple. The results showed that application of Spd at 1 ×10^-5mol·L^-1 at full bloom could increase fruit set and the contents of GA3 and ZR in the flowers and fruitlets of Red Fuji apple , but reduced IAA and ABA contents. MGBG basically had an opposite effects. The Spd treatment could also increase endogenous Put , Spd and Spm contents in the fruitlet of Red Fuji apple , but reduced their contents in the flowers , MGBG treatment decreased the endogenous Spd contents but increased Put contents.
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• Classification Study of Xinjiang Peach Using RAPD Markers
• Cheng Zhongping;Chen Zhiwei;Hu Chungen;Deng Xiuxin;and Luo Zhengrong
• Acta Horticulturae Sinica. 2001, 28(3): 211-217.
• Abstract ( 1209 ) HTML ( 1146 ) PDF (342KB) ( 1146 ) 　　　
• 203 peach materials were amplified with elected 22 102mer primers , using RAPD. Especially , 5 types of Xinjiang Peach were evaluted. It is indicates that study Xinjiang peach should be a variant of Prunus persica and named as P. persica var. ferganensis in Latin language.
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• Changes of Lipoxygenase Activity and Memberane Fatty Acid Composition in Postharvest Ripening Peach (Prunus persica L.) Fruit
• Wu Min;Chen Kunsong;Zhang Shanglong;and Wu Ping
• Acta Horticulturae Sinica. 2001, 28(3): 218-222.
• Abstract ( 1450 ) HTML ( 1783 ) PDF (207KB) ( 1783 ) 　　　
• A peach cultivar‘Hujingmilu’was used to investigate the changes of LOX activity , ethylene production and memberane fatty acid composition. It was found that the major fatty acids in the ripening peach fruit included palmitic acid (16∶0) , stearic acid (18∶0) , oleic acid (18∶1) , linoleic acid (18∶2) and linolenic acid (18∶3) . There were remarkable changes in the proportion of
  oleic acid , linoleic acid and linolenic acid , especially linoleic acid and linolenic acid , while no such notable changes in the proportion of palmitic acid and stearic acid during fruit ripening at 20 ℃. Linolenic acid was mainly used as a LOX catalyzing substrate before LOX self-propagation during fruit
  ripening at 20 ℃. LOX might play a role in memberane lipid peroxidation which participated in fruit ripening and softening. The significance of these results was discussed.
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• Quantitative Genetics Analysis and Multiple Character Selection on Ginkgo Seed
• Xing Shiyan;Zhang Zhongqing;Zhang Youpeng;Meng Lingguo;and Zhang Rui
• Acta Horticulturae Sinica. 2001, 28(3): 223-228.
• Abstract ( 1329 ) HTML ( 1029 ) PDF (342KB) ( 1029 ) 　　　
• China possesses more than 70 % ginkgo resources in the world. The primary problems existing in ginkgo cultivation are : (1) no big-grain and fine nucleus-used cultivars ; (2) no known genetic law of primary characters ginkgo seed. The purposes of this research are to collect 50 nucleusused cultivars from 12 provinces in China , to carry out genetic analysis for economical character and
  multiple character selection on ginkgo seed. The results of analysis show that maxmum single nucleus weight , stone mass/ fruit mass ( %) and kernel mass/ stone mass ( %) are 3. 89 g , 33. 58 % and 82. 49 % , respectively. The heritability ( h2) of single nucleus mass is 0. 9759. The genetic correlation coefficient ( rG) between nucleus2shape coefficient and single nucleus mass , and direct genetic path coefficient ( PY·i) of single fruit mass to single stone mass are 0. 9986 and 1. 407 , respectively. Twelve provenances from China are divided into four classes. Three fine nucleus-used cultivars are selected out through index or direct selection methods from fifty nucleus-used cultivars in China. The theory of nucleus2used cultivar selection was studied in this paper.
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• Effects of Low Temperature and Low Light Intensity Stress on Photosynthesis in Seedlings of Different Cucumber Varieties
• Wang Yongjian;Zhang Haiying;Zhang Feng;Xu Yong;Cao Wanhong;and Kang Guobin
• Acta Horticulturae Sinica. 2001, 28(3): 230-234.
• Abstract ( 1527 ) HTML ( 1979 ) PDF (208KB) ( 1979 ) 　　　
• Under the stress of low temperature (15 ℃) and low light intensity (100μE·m^-2·s^-1) , the photosynthetic light compensation point of cucumber seedlings went up at the beginning of treatment , some parameters of chlorophyll a fluorescence such as Fv/ Fm , qp , ΦPS Ⅱ ( Y) decreased obviously , and the activity of RuBPCase was also reduced. This results indicated that the primary light energy conversion of PS Ⅱ, electron transport and carbon assimilation of cucumber seedlings had been inhibited , thus the capacity of its utilizing low light intensity went down. However , after
  the induction of stress , the adaptability of cucumber seedlings increased , and the above parameter reversed the level before stress treatment . The results also showed that the adaptability of greenhouse varieties was higher than that of open field varieties.
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• A Study on the Regulating Self􀀁incompatibility of Brassica oleracea L. by Inhibitor or Activitor of Protein Kinase
• Lv Jun;Zhu Liquan;and Wang Xiaojia
• Acta Horticulturae Sinica. 2001, 28(3): 235-239.
• Abstract ( 1377 ) HTML ( 1073 ) PDF (175KB) ( 1073 ) 　　　
• A study on regulating self􀀁incompat ibility of Brassica oleracea L. by inhibitor
  ( quercetin ) or activitor ( PMA) of protein kinase was couducted. The result showed that the quercetin increased the index of self􀀁compat ibility, even turned the self􀀁incompatible line to self-compatible line; PMA reduces the index of self-compatibility, even turned the self􀀁compatible line to self-incompatible line. This is a new method for regulating the self-incompatibility of cabbage.
  
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• A Study on Daily Variation of Photosynthesis in Greenhouse Butter head Lettuce
• Li Pingping;Hu Yongguang;Zhao Yuguo;andMao Hanping
• Acta Horticulturae Sinica. 2001, 28(3): 240-245.
• Abstract ( 1310 ) HTML ( 1938 ) PDF (211KB) ( 1938 ) 　　　
• The tests of daily variation of photosynthetic rate of butterhead lettuce grown in green house are conducted. The results show that the daily variation of photosynthetic rate is a two peak curve in sunny days in summer and autumn season. While in winter with lower temperature, it is a
  one-peak curve pattern. In the cloudy and raining days, the change of photosynthesis just follow the change of radiat ion. The photosynthesis maintaining at its high value is not more than 3- 4 hours within a day. If the radiation and temperature cannot meet the need of crop in the morning, it is still possible to produce a photosynthet ic peak in the afternoon with suitable environment factors for the crop photosynthesis.
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• Genetic Transformation and Plant Regeneration of Lettuce with SweetProtein Gene MBLII
• Liu Jingmei;Chen Daming;and Chen Hang
• Acta Horticulturae Sinica. 2001, 28(3): 246-250.
• Abstract ( 1431 ) HTML ( 1046 ) PDF (201KB) ( 1046 ) 　　　
• The sweet protein gene MBL II was introduced into lettuce by co􀀁culturing excised cotyledon explants with Agrobacterium tumefaciens . The Agrobacterium strain LBA4404 contained the binary vector pLX10, which carried sweet protein gene MBL II, select ive gene NPTII and reporter
  gene GUS . The best transformation efficiency was obtained when explants were co􀀁cultured with Agrobacterium for 3 days and cultured on the selective medium containing 50 mg/ L kanamycine ( shoots regeneration rate 58. 3%, albino rate 29% ) . Roots were induced in all adventitious shoots
  on MSII (MS+ NAA 0. 05 mg/ L+ Kan 50 mg/ L+ carbencillin 300 mg/L) . The transgenic nature of regenerants were demonstrated by plant growth on medium containing kanamycine, GUS histochemical assay and PCR amplification. 7. 6% of all Kanamycine resistant shoots was GUS positive.
  Southern blot results confirmed that the MBL II gene had been incorporated into the genome of lettuce.
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• Isolation of a cDNA Fragment Homologous to the Large Subunit Geneof ADP-Glucose Pyrophosphorylase from Amorphophallus
• Zhang Xingguo;Yang Zheng’an;Du Xiaobing;Li Zhengguo;and Liu Peiying
• Acta Horticulturae Sinica. 2001, 28(3): 251-254.
• Abstract ( 1235 ) HTML ( 1119 ) PDF (188KB) ( 1119 ) 　　　
• PCR primers were designed based on the consensus domain of ADP-glucose pyrophos-phorylase. A cDNA fragment was amplified from the total RNA isolated from the yang corm of Amorphophallus albus via RT-PCT and cloned into plasmid pUC18. The determined sequence showed this cDNA fragment was homologous to the large subunit gene of ADP-glucose yrophosphorylase.
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• Morphological Differentiation of Male Flower Bud of Ginkgo biloba L.
• Zhang Wanping;Shi Jikong;Fan Weiguo;and Wen Xiaopeng
• Acta Horticulturae Sinica. 2001, 28(3): 255-258.
• Abstract ( 1325 ) HTML ( 1205 ) PDF (136KB) ( 1205 ) 　　　
• Male flower bud morphological differentiation of Ginkgo biloba L. begins in early June and continues to next March in Guizhou area. It can be devided into five stages : vegetative stage , initial differentiation stage , microsporagia differentiation stage , microsporocytes differentiation
  stage and microspores differentiation stage.
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研究报告

• A Study on the Relationships between Acid Invertase , Sucrose Synthase andSucrose Metabolism in ‘Red Fuji’Apple Fruit
• Wang Yongzhang and Zhang Dapeng
• Acta Horticulturae Sinica. 2001, 28(3): 256-261.
• Abstract ( 1523 ) HTML ( 2478 ) PDF (150KB) ( 2478 ) 　　　
• The changes of sugar accumulation and related enzyme activities in sugar metabolism of ‘Red Fuji’apple fruit showed that the activities of soluble and cell wall2bound acid invertase had significant negative correlation with
  soluble sugar contents in the early stage of fruit development. However , the activity of sucrose synthase showed a highly positive correlation with sucrose accumulation in the late development stage. It is concluded that the sucrose metabolism in apple fruit may be mainly mediated by acid invertase and sucrose synthase.
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• The Development of Seed and Floral Organ on the Primary and Secondary Flower Clusters of ‘Kyoho’ Grapevine
• Hu Jianfang;He Haiyang;Zhang Dapeng;and Matsui Hiroyuki
• Acta Horticulturae Sinica. 2001, 28(3): 262-264.
• Abstract ( 1432 ) HTML ( 1029 ) PDF (164KB) ( 1029 ) 　　　
• The studies of the formation and development of the sexual organs on the primary and secondary flower cluster of 􀀂Kyoho grapevine were conducted, aiming at elucidating the mechanism of the low seed production on the
  primary flower clusters. The results showed that the pollen of the florets of the secondary flower clusters was smaller, though the percentage of pollen stained by acetocarmine on florets of both flower clusters was same, the percentage of germination on agar of the secondary flower clusers was higher than that of the primary flower clusters. The result indicated that the pollen quality was not related to flower shatter. The embryo-sac evelopment with function cell in the pistils of the secondary flower clusters was more favorable than that of the primary flower clusters. At anthesis, a large number of abnormal embryo􀀁sac were observed in the ovaries of the primary flower clusters. As a result, at anthesis, a large number of abnormal embryo􀀁sac may suppress fertilization and lead to lower seed.
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• A Study on Peroxidase Isoenzymes of Leaves in Ziziphus spinosus Hu. and Seventeen Varieties of Z. juj uba Mill.
• Su Dongmei;Chen Shujun;and Bi Fangcheng
• Acta Horticulturae Sinica. 2001, 28(3): 265-267.
• Abstract ( 1522 ) HTML ( 1008 ) PDF (182KB) ( 1008 ) 　　　
• Peroxidase isoenzymes of leaves in Ziz ip hus spinosus Hu. and 17 varieties of Z. jujuba Mill. were analyzed by means of perpendicular􀀁plate type of polyacrylamide gel electrophoresis. Fuzzy cluster analysis was conduc􀀁
  ted based on the similarity coefficients of the isoenzyme zymogram, using the R􀀁chain link method of similarity relation ship. Z . spinosus Hu. and 17 varieties of Z. jujuba Mill. were classified into several groups. and the relationship among the groups was discussed. From the phenotype of molecular enzyme level, it has been proved that Z. spinosus is the primeval species of the cultivated Z. jujuba, which is the result of a longtime evolution. But both species are closely related to each other.
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• A Study on the Taxonomy of Malus trilobata Schneid.
• Cheng Minghao;Zhou Zhiqin;Deng Hongping;and Li Xiaolin
• Acta Horticulturae Sinica. 2001, 28(3): 268-269.
• Abstract ( 1445 ) HTML ( 1150 ) PDF (103KB) ( 1150 ) 　　　
• Basing on the research advancement of taxonomy for Malus trilobata Schneid. , using the theories and methods of phylogeny􀀁phytogeography, the authors found that Malus trilobata developed independently from Malus laoensis Chev. , one of the most primitive species of Malus Miller, after they separated from a homogeneous flora in early time. Malus trilobata is one of the most closely related taxa of the genus Malus Miller and it should be originated in theCretaceous period. As a conclusion, the author suggested that Malus trilobata Schneid. be named Cormus Decisne.
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• Effects of Cytokinins on Shoot Organogenesis from Cotyledon of Pepper
• Li Yinghui;Li Yan;Hang Xiaoming;and Wang Peng
• Acta Horticulturae Sinica. 2001, 28(3): 270-272.
• Abstract ( 1184 ) HTML ( 1330 ) PDF (122KB) ( 1330 ) 　　　
• Three cytokinins BA, ZT, and KT were tested individually in series of experiments to determine their role in inducing shoot bud formation. The result showed that BA 4. 0 mg。L^-1 or ZT 3. 0 mg。L^-1 was most favourable to shoot bud formation, KT was proved in effective in inducing shoot bud formation.
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• Stem Cuttings of Delphinium‘Schloss Wilhelmschohe’
• Kuang Jian;Guan Kaiyun;Li Jingxiu;Li Zhijian;and Sun Weibang
• Acta Horticulturae Sinica. 2001, 28(3): 273-274.
• Abstract ( 1262 ) HTML ( 1040 ) PDF (209KB) ( 1040 ) 　　　
• The experiment showed that Delphinium‘Schloss Wilhelmschohe’can be propagated by stem cuttings. The ripe or semi-ripe stems or branches of 12 - 15 cm in length cut into the medium of perlite∶humus∶sand = 1∶1∶2
  (volume) from Spring to Summer at Kunming , and the excellent rooted cuttings will be get 40 days later. The IBA 5 - 10 mg·L^-1 treatment can stimulate the root formation.
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新品种

• Development of Microbiological Nitrogen and Effects on Yield and Traits of Brassica chinensis
• Zhao Peijie
• Acta Horticulturae Sinica. 2001, 28(3): 275-275.
• Abstract ( 1150 ) HTML ( 836 ) PDF (70KB) ( 836 ) 　　　
• The microbiological nitrogen is a newfertilizer synergist developed fromferment products of Trichoderma harziarum. Result showed the hog manure added with microbiological nitrogen could promote the plant height , leaf leangth and leaf breadth and increase the yield of Brassica chinensis.
  
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• 'Qinzhou red litchi'——A New Big Fruit form Variety of Litchi
• Peng Hongxiang;Li Yunchang;Huang Dejian;Su Weiqiang;and Liu Rongguang
• Acta Horticulturae Sinica. 2001, 28(3): 276-276.
• Abstract ( 1523 ) HTML ( 888 ) PDF (151KB) ( 888 ) 　　　
• The new variety‘Qinzhou’ red litchi was a natural bastard mutant of ‘Black ’leaf litchi, and showed excellent characters with early fruit, high yield, big fruit form, bright red rind and excellent quality. lts hypoplastic
  seed rate was 36. 15%.
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• ‘Zaoheibao’ ——— An Early-maturing , Good Quality and Large Berry Grape Variety
• Chen Jun;Tang Xiaoping;Li Dengke;Ma Xiaohe;and Dong Zhigang
• Acta Horticulturae Sinica. 2001, 28(3): 277-277.
• Abstract ( 1578 ) HTML ( 1026 ) PDF (175KB) ( 1026 ) 　　　
• ‘Zaoheibao’grape is an early maturing tetraploid variety and selected from colchicine treated hybrid seeds of ‘Guibao’(2x) בZaomeigui’(2x) . It has good quality and high yields with big , purple2black fruits.
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• Breeding of ‘Xiangyan No. 14’by Male Sterility Line of Pepper
• Zou Xuexiao;Zhou Qunchu;Dai Xiongze;Ma Yanqing;Li Xuefeng;Zhang Zhuqing;Liu Rongyun;and Cheng Wenchao
• Acta Horticulturae Sinica. 2001, 28(3): 278-278.
• Abstract ( 1215 ) HTML ( 796 ) PDF (134KB) ( 796 ) 　　　
• The female parent 9704A of ‘Xiangyan No. 14’is male sterility line bred from a sterile plant found in ox2horn pepper No. 21 and the male parent 5904 is an ox-horn pepper inbred line selected from a landrace in Guangxi ,
  with large fruit and thick wall. The late pungent F₁ hybrid is suitable for upper and middle region of Yangze river as trans-summer production and South China as winter open field production. The ox-horn shaped and straight fruit with smooth appearance and thick skin is suitable for storage and transportation. It can be harvested both light green (ripening) and bright red (ripened) . The average yield is 49. 5 - 60 t/ hm² with fruit weight proximately 38 grams.
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• New Chinese Cabbage Hybrid ‘Qianbai No. 1’
• Zhao Daqin;Wang Daiyi;Li Ying;and Tu Dan
• Acta Horticulturae Sinica. 2001, 28(3): 279-279.
• Abstract ( 1235 ) HTML ( 759 ) PDF (60KB) ( 759 ) 　　　
• ‘Qianbai No. 1’ is a middle maturity Chinese cabbage hybrid. It can be harvested 82 days after sowing. It is suitable for growing in autumn and winter. The average yield is about 60 t/ hm². The variety has excellent
  quality, multiple disease resistances, wide adaptability, high yield and good marketing characteristics.
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• A New Cucumber Cultivar‘Shannong No. 5’
• Cao Chenxing;Zhang Qipei;Guo Hongyun;and Zhang Song
• Acta Horticulturae Sinica. 2001, 28(3): 280-280.
• Abstract ( 1300 ) HTML ( 925 ) PDF (65KB) ( 925 ) 　　　
• Shannong No. 5 is a new cultivar for protected cultivation. It is suitable for solar greenhouse production in Huabei district. Its plant grows vigorously, and it yields 120 tons per hm² . Average fruit is about 35 cm length and 150 g weight. Fruits are green, sweet and crispy. It is tolerant to chilling and low light. It is resistant to fusarium wilt, downy mildew, powdery mildew and scab.
  
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• New Begonia Varieties——‘White King’, ‘Silvery Pearl’ and ‘Tropical Girl’
• Tian Daike;Guan Kaiyun;Li Jingxiu;Xiang Jianying;and Guo Ruixian
• Acta Horticulturae Sinica. 2001, 28(3): 281-282.
• Abstract ( 1764 ) HTML ( 1035 ) PDF (98KB) ( 1035 ) 　　　
• ‘White King’is natural variety of Begonia rex . It is rhizomatic herb with white spot area on the green leaf surface and white flower and green fruits. Silvery Pearl! is a variety of B . hemasleyana. It is a beautiful ornamental plant with stable silvery pear-like spots on leaf surface different from the latter, but its flower is usually a little smaller than that of B . emasleyana. Tropical Girl! is the new variety selected from B . pr otrata. It is a tropical plant with white spots on leaf surface and pink or white flower.
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