Abstract: It was observed that the final disease severity was mainly affected by sporulation medium，growing time of banana plantlet on medium for interaction system（MIS）before in vitro inoculation，components of MIS medium，and pathogenicity of Fusarium oxysporum f. sp. cubense（Foc）tropical race 4（TR4）strain. The effects of the four factors on final disease severity were studied by using orthogonal experimental design. Logistic regression model analysis of data showed that pathogenicity of Foc TR4 strain was the most important factor（P < 0.001），followed by sporulation medium（P < 0.05），components of MIS medium（P < 0.05），and growing time of banana plantlet on MIS medium before in vitro inoculation. The optimum factor combination for rapid screening was the third-generation rejuvenated strain，potato dextrose water meduim，half-strength Murashige and Skoog salts without organic elements， and 7-day growth of banana plantlet on MIS before in vitro inoculation，which was consistent with the result of verification test.

Key words: banana, Fusarium oxysporum f. sp. cubense, screening for resistance, in vitro inoculation, orthogonal experimental design