Identification of Differential Metabolic Pathways and Key Regulatory Genes in the Two Flower Bud Differentiation Processes of Magnolia liliiflora

doi:10.16420/j.issn.0513-353x.2019-0963

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (8): 1490-1504.doi: 10.16420/j.issn.0513-353x.2019-0963

• Research Papers • Previous Articles Next Articles

Identification of Differential Metabolic Pathways and Key Regulatory Genes in the Two Flower Bud Differentiation Processes of Magnolia liliiflora

CHENG Shaoyu，Xuan Lingjuan，DONG Bin，GU Cuihua，SHEN Yamei*，ZHANG Mingru，DAI Mengyi，WANG Zhuowei，ZHANG Yingjia，and LU Danying

Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants，School of Landscape Architecture，Zhejiang A & F University，Hangzhou 311300，China

Online:2020-08-25 Published:2020-08-25

Abstract

Abstract: Magnolia liliiflora‘Hongyuanbao’blooms in spring and summer，transcriptome sequencing and metabolome sequencing were analyzed to explore the differential metabolic pathways and key regulatory genes during second flower bud differentiation processes of‘Hongyuanbao’. 43 257 unigenes and 569 metabolites were identified. Thirty-five floral development genes were involved in the twice flower bud differentiation. Combine transcriptomic with metabolomics analysis，4 074 genes were involved in the middle stage of second flower bud differentiation. Meanwhile，sucrose and 3-cyanoalanine were significantly up-regulated in the second time，and methylmalonic acid was significantly down-regulated（|log2FC| ≥ 2）. The correlation value of the corresponding differential genes was |PCC| > 0.80 and P < 0.05 in four KEEG pathways. The correlation between trehalose and seven differential genes were |PCC| > 0.80 in the sucrose and starch metabolic pathway. CCA analysis showed that comparison between the middle stage of twice flower bud differential process identified three KEGG pathways：ko01200 carbon metabolism，ko00630 glyoxylic acid and dicarboxylic acid metabolism and ko02010ABC transport protein. The expression of genes related to floral development，6 genes（MlCOL9，MlGA9，MlGAI，MlSPL4，MlSPY，MlSVP）were selected from 35 different genes for qRT-PCR verification，which verified the accuracy of transcriptome sequencing results. In summary，this study showed that the secondary flower bud differentiation of‘Hongyuanbao’was produced by the combined influence of photoperiod pathway，vernalization pathway，age pathway and gibberellin pathway and 8 metabolic pathways，and the sucrose and trehalose played important role in it.

Key words: Magnolia liliiflora, secondary flowering, differential gene, differential metabolite, qRT-PCR

CLC Number:

S 685.15

CHENG Shaoyu，Xuan Lingjuan，DONG Bin，GU Cuihua，SHEN Yamei*，ZHANG Mingru，DAI Mengyi，WANG Zhuowei，ZHANG Yingjia，and LU Danying. Identification of Differential Metabolic Pathways and Key Regulatory Genes in the Two Flower Bud Differentiation Processes of Magnolia liliiflora[J]. ACTA HORTICULTURAE SINICA, 2020, 47(8): 1490-1504.

[1]	ZHANG Qiuyue, LIU Changlai, YU Xiaojing, YANG Jiading, FENG Chaonian. Screening of Reference Genes for Differentially Expressed Genes in Pyrus betulaefolia Plant Under Salt Stress by qRT-PCR [J]. Acta Horticulturae Sinica, 2022, 49(7): 1557-1570.
[2]	WANG Hong, YANG Wangli, LIN Jing, YANG Qingsong, LI Xiaogang, SHENG Baolong, CHANG Youhong. Comparative Metabolic and Transcriptomic Analysis of Ripening Fruit in Pear Cultivars of‘Sucui 1’‘Cuiguan’and‘Huasu’ [J]. Acta Horticulturae Sinica, 2022, 49(3): 493-508.
[3]	ZHANG Rui, ZHANG Xiayi, ZHAO Ting, WANG Shuangcheng, ZHANG Zhongxing, LIU Bo, ZHANG De, WANG Yanxiu. Transcriptome Analysis of the Molecular Mechanism of Saline-alkali Stress Response in Malus halliana Leaves [J]. Acta Horticulturae Sinica, 2022, 49(2): 237-251.
[4]	ZHANG Zhiqiang, LU Shixiong, MA Zonghuan, LI Yanbiao, GAO Caixia, CHEN Baihong, MAO Juan. Bioinformatic Identification and Expression Analysis of Candidate Genes of LIM Protein Family in Strawberry Under Abiotic Stresses [J]. Acta Horticulturae Sinica, 2021, 48(8): 1485-1503.
[5]	ZHU Junfei, LI Xin, DONG Kangting, TANG Zhifei, BIAN Xiuju, WANG Lihong, LI Huibin, SUN Xinbo. Effect of Creeping Bentgrass Small Heat Shock Protein AsHSP26.8a on Transgenic Photosynthesis in Arabidopsis thaliana [J]. Acta Horticulturae Sinica, 2021, 48(8): 1619-1625.
[6]	JIA Bing, GUO Guoling, WANG Youyu, YE Zhenfeng, LIU Li, LIU Pu, HENG Wei, ZHU Liwu. Studies on the Regreening Mechanism of the Iron-deficiency Chlorosis Leaves induced by GA₃ in‘Whangkeumbae’（Pyrus pyrifolia Nakai） [J]. Acta Horticulturae Sinica, 2021, 48(2): 254-264.
[7]	MA Lulin, DUAN Qing, CUI Guangfen, DU Wenwen, JIA Wenjie, WANG Xiangning, WANG Jihua, CHEN Fadi. Selection and Validation of Reference Genes for qRT-PCR Analysis of the Correlated Genes in Flower Pigments Biosynthesis Pathway of Anemone obtusiloba [J]. Acta Horticulturae Sinica, 2021, 48(2): 377-388.
[8]	JIANG Mengqi, XUE Xiaodong, SU Liyao, CHEN Yan, ZHANG Shuting, LI Xiaofei, WANG Peiyu, ZHANG Zihao, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of TCP Family in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2021, 48(12): 2481-2496.
[9]	MAO Juan，LU Shixiong，WANG Ping，MA Zonghuan，LIU Tao，HE Honghong，NAI Guojie，ZHANG Zhiqiang，and CHEN Baihong*. Analysis of Gene Characteristic and Expression Pattern of LIM Family in Grape [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1277-1288.
[10]	ZHOU Tao1,2，WANG Juan2，WANG Lulu1，WANG Baike2，HU Jiahui1,2，LAN Haiyan1,，and YU Qinghui2,. Cloning and Prokaryotic Expression Analysis of a Transcription Factor Gene SlWRKY16 in Tomato [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1312-1322.
[11]	FU Yajuan1,2，CHEN Xiating1，QIAO Jie1,2，WANG Jing1,2，LI Wenjing1，and HOU Xiaoqiang1,2,*. Molecular Cloning and Expression Characterization of Cyclophilin Gene（DoCyP）in Dendrobium officinale [J]. ACTA HORTICULTURAE SINICA, 2020, 47(3): 581-589.
[12]	LIU Tao，WANG Pingping，HE Honghong，LIANG Guoping，LU Shixiong，CHEN Baihong，and MAO Juan*. Identification and Expression Analysis of CIPK Gene Family in Strawberry [J]. ACTA HORTICULTURAE SINICA, 2020, 47(1): 127-142.
[13]	LU Shixiong，WANG Ping，HE Honghong，LIANG Guoping，MA Zonghuan，QIAO Yali，WU Yuxia，CHEN Baihong，and MAO Juan. Bioinformatics Identification and Expression Analysis of Grape Trihelix Transcription Factor Family [J]. ACTA HORTICULTURAE SINICA, 2019, 46(7): 1257-1269.
[14]	WEN Beibei1，LUO Yong1，LIU Dongmin1，ZHANG Xiangna1，LI Juan1,2,3，WANG Yingzi1,2,3，WANG Kunbo1,2,3,，and HUANG Jian’an1,2,3,. Identification and Expression Profiling Analysis of TCP Family Genes Involved in Growth and Development in Camellia sinensis [J]. ACTA HORTICULTURAE SINICA, 2019, 46(12): 2369-2382.
[15]	YIN Chaomin1，CHEN Yeye2，LU Jing2，and MA Aimin2,. Cloning and Expression Analysis of Lectin Genes from Pleurotus ostreatus* [J]. ACTA HORTICULTURAE SINICA, 2017, 44(1): 131-138.

Identification of Differential Metabolic Pathways and Key Regulatory Genes in the Two Flower Bud Differentiation Processes of Magnolia liliiflora

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments