Genomic Identification of Jujube AP2/ERF Transcription Factors and Their Expression Pattern During Jujube Witches’ Broom Pathogenesis Process

doi:10.16420/j.issn.0513-353x.2019-0965

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (8): 1463-1474.doi: 10.16420/j.issn.0513-353x.2019-0965

• Research Papers • Previous Articles Next Articles

Genomic Identification of Jujube AP2/ERF Transcription Factors and Their Expression Pattern During Jujube Witches’ Broom Pathogenesis Process

LI Jidong*，NI Jing*，YE Xia，CHEN Peng，TAN Bin，ZHANG Mengyang，ZHENG Xianbo，ZHANG Yu，and FENG Jiancan**

Henan Agricultural University，Henan Key Laboratory of Fruit and Cucurbit Biology，Zhengzhou 450002，China

Online:2020-08-25 Published:2020-08-25

Abstract

Abstract: In this study，a total of 175 and 164 jujube AP2 / ERF transcription factors were obtained from the plant transcription factor database and the NCBI database by bioinformatics methods，respectively. Duplicated sequences were deleted after sequences aligning by DNAMAN. ERF domain were confirmed by SMART software. Finally，145 AP2/ERF transcription factors were identified，of which 116，23，and 5 members were divided into ERF，AP2，RAV subfamily，respectively，including another independent gene. The jujube AP2/ERF transcription factors were featured with amino acid numbers among 111–692，molecular weight among 12 446.87–76 154.10 D，and isoelectric point among 4.31–10.11. 105 jujube AP2/ERF genes were mapped to the 12 jujube chromosomes and the others were not. Jujube witches’ broom（JWB） diseased buds were grafted to healthy 1-year-old seedlings and 4-year-old trees to perform an infection process. The response of AP2/ERF transcription factors to JWB phytoplasma was analyzed according to the transcriptome datas and qRT-PCR. Results showed that the expressing level and number of AP2/ERF were different in 6 sampling stages. The expressing level of 48 AP2/ERF were significantly different in 6 periods. Among them，ZjAP2*9，ZjERF49 and ZjERF91 were the most important AP2/ERF transcription factors in response to the phytoplasma.

Key words: jujube, jujube witches’ broom, phytoplasma, AP2/ERF transcription factors

CLC Number:

S 665.1

LI Jidong*，NI Jing*，YE Xia，CHEN Peng，TAN Bin，ZHANG Mengyang，ZHENG Xianbo，ZHANG Yu，and FENG Jiancan**. Genomic Identification of Jujube AP2/ERF Transcription Factors and Their Expression Pattern During Jujube Witches’ Broom Pathogenesis Process[J]. ACTA HORTICULTURAE SINICA, 2020, 47(8): 1463-1474.

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Genomic Identification of Jujube AP2/ERF Transcription Factors and Their Expression Pattern During Jujube Witches’ Broom Pathogenesis Process

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