Cloning and Functional Analysis of BcAMT1;4 Gene Promoter in Flowering Chinese Cabbage

doi:10.16420/j.issn.0513-353x.2019-0518

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (4): 675-685.doi: 10.16420/j.issn.0513-353x.2019-0518

• Research Papers • Previous Articles Next Articles

Cloning and Functional Analysis of BcAMT1;4 Gene Promoter in Flowering Chinese Cabbage

ZHU Yunna1,2，ZHOU Xiaoxia1，LIANG Wenwen1，LIU Jianguo2，CHEN Riyuan1,*，and SONG Shiwei1,*

1College of Horticulture，South China Agricultural University，Guangzhou 510462，China；2College of Yingdong Agricultural Science & Engineering，Shaoguan University，Shaoguan，Guangdong 512005，China

Online:2020-04-25 Published:2020-04-25

Abstract

Abstract: In the present study，ammonium transporter gene BcAMT1;4 promoter sequence with upstream length of 2 086 bp for ATG was obtained from flowering Chinese cabbage（Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee）using genomic walking. Bioinformatics analysis indicated that the promoter contains multiple cis-acting elements associated with light signals，hormone signals，stress response，and tissue-specific expression. To further analyze the promoter function of BcAMT1;4，a recombinant vector of BcAMT1;4 promoter and GUS was constructed and transformed into Arabidopsis by Agrobacterium tumefaciens. Analysis of T3 transgenic Arabidopsis tissues with GUS staining showed that GUS was mainly expressed in leaf，while expressed slightly in root，stem，and flower. Also，the activity of BcAMT1;4 promoter was affected by nitrogen source forms in Arabidopsis. Nitrogen starvation induced the GUS activity in transgenic Arabidopsis，however，the GUS activity was inhibited by 0.25 mmol ? L-1 NO3-，NH4+，or NH4NO3 treatment. In conclusion，the results showed that BcAMT1;4 might play an important role in regulating ammonium nutrient in leaf of flowering Chinese cabbage.

Key words: flowering Chinese cabbage, nitrogen, ammonium transporter, promoter, GUS

CLC Number:

S 634.5

ZHU Yunna1,2，ZHOU Xiaoxia1，LIANG Wenwen1，LIU Jianguo2，CHEN Riyuan1,*，and SONG Shiwei1,*. Cloning and Functional Analysis of BcAMT1;4 Gene Promoter in Flowering Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 675-685.

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Cloning and Functional Analysis of BcAMT1;4 Gene Promoter in Flowering Chinese Cabbage

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