Screening and Identification of Bacillus Antagonist Against Anthracnose of Jujube and Analysis of Its Antifungal Substances

doi:10.16420/j.issn.0513-353x.2019-0052

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (12): 2406-2414.doi: 10.16420/j.issn.0513-353x.2019-0052

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Screening and Identification of Bacillus Antagonist Against Anthracnose of Jujube and Analysis of Its Antifungal Substances

LI Jiawei，XU Lanyi，WANG Dongshuang，Lü Lijie，CHEN Xiaomeng，and ZHANG Dongdong*

College of Life Science，Agricultural University of Hebei，Baoding，Hebei 071000，China

Online:2019-12-25 Published:2019-12-25

Abstract

Abstract: Bacillus strains isolated from jujube fruits with significant antagonistic activity against anthracnose was screened by confrontation culture method. The control effect of antagonistic bacteria against anthracnose was tested by in vivo experiment using winter jujube fruits. The activity and structure of the antifungal substance from the fermentation supernatant of the antagonistic bacterium were tested by inhibition zone method and liquid chromatography-mass spectrometry（LC–MS），respectively. Strain Tj-19 which exhibited significant antagonistic activity against jujube anthracnose was identified as Bacillus amyloliquefaciens based on the morphological observation，physiological and biochemical characteristics and 16S rDNA and gyrB sequence analysis. The biocontrol effect of Tj-19 bacteria cell and its fermentation supernatant on anthracnose of winter jujube fruit reached 74.9% and 75.8%, respectively. The main antifungal substances from the fermentation broth of strain Tj-19 were Iturin A and Iturin B.

Key words: jujube, anthracnose, Bacillus, antagonism, screening, antifungal substance

CLC Number:

S 665.1

LI Jiawei，XU Lanyi，WANG Dongshuang，Lü Lijie，CHEN Xiaomeng，and ZHANG Dongdong*. Screening and Identification of Bacillus Antagonist Against Anthracnose of Jujube and Analysis of Its Antifungal Substances[J]. ACTA HORTICULTURAE SINICA, 2019, 46(12): 2406-2414.

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Screening and Identification of Bacillus Antagonist Against Anthracnose of Jujube and Analysis of Its Antifungal Substances

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