Abstract: In order to study the generation and scavenging of reactive oxygen species（ROS）in leaves of grape strains with different salt tolerance under salt stress，the salt-tolerance tissue culture hybrids（A15 and A17）of Vitis amurensis Rupr.‘Zuoshan 1’× SO4 were used as materials to be irrigated with 100 mmol · L-1 NaCl，and tissue culture 1103P was used as negative control. O2- production rate，H2O2 content，MDA content，relative electrical conductivity，chlorophyll content，antioxidant enzyme activities and antioxidant substances were determined. The results showed that，under salt stress，SOD，CAT and POD activities，AsA and GSH content and APX and GR activities in leaves were higher in salt-tolerance hybrids A15 and A17，ROS and MDA content were lower，and the increment of relative electrical conductivity was less，the structure of chloroplast was integrated，and the chloroplast content was higher；while the overproduction of ROS and MDA damaged the membrane integrity and reduced chloroplast content in 1103P. Salt-tolerance hybrids A15 and A17 remained high antioxidase activity and AsA-GSH cycle efficiency，reduced the damage of membrane caused by lipid peroxidation，maintained the structure and function integrity of chloroplast.

Key words: grape, rootstock, hybrid strain, NaCl stress, leaf, ROS metabolism