Primary Analysis of the Function of bZIP Transcription Factor Gene MdAREB2 in Apple

doi:10.16420/j.issn.0513-353x.2017-0195

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (1): 1-10.doi: 10.16420/j.issn.0513-353x.2017-0195

• Research Papers • Next Articles

Primary Analysis of the Function of bZIP Transcription Factor Gene MdAREB2 in Apple

LIU Yajing，MA Qijun，LU Jing，HAO Yujin，and YOU Chunxiang*

State Key Laboratory of Crop Biology，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region, Ministry of Agriculture，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China

Online:2018-01-25 Published:2018-01-25

Abstract

Abstract: A bZIP transcription factor gene MdAREB2（MDP0000248567）was cloned from‘Gala’apple（Malus × domestica Borkh.）. The evolutionary tree analysis indicated that MdAREB2 had the very high homology with AtAREB2. The promoter of MdAREB2 was analyzed and predicted by PlantCare databases，and the results showed that the promoter sequence contained abscisic acid response element ABRE. Quantitative real-time PCR showed that MdAREB2 was significantly induced by ABA. This study found that with 0.5 μmol · L-1 ABA and 2 μmol · L-1 ABA treatment，excessive expression of MdAREB2 in mutant abi5 could restore its sensitivity to ABA in seed germination stage. In the seedling growth stage，with 20 μmol · L-1 ABA treatment，excessive expression of MdAREB2 in mutant abi5 could partially restore its sensitivity to ABA.

Key words: apple, bZIP, transcription factor, MdAREB2, function analysis

LIU Yajing，MA Qijun，LU Jing，HAO Yujin，and YOU Chunxiang*. Primary Analysis of the Function of bZIP Transcription Factor Gene MdAREB2 in Apple[J]. ACTA HORTICULTURAE SINICA, 2018, 45(1): 1-10.

[1]	YE Zimao, SHEN Wanxia, LIU Mengyu, WANG Tong, ZHANG Xiaonan, YU Xin, LIU Xiaofeng, and ZHAO Xiaochun, . Effect of R2R3-MYB Transcription Factor CitMYB21 on Flavonoids Biosynthesis in Citrus [J]. Acta Horticulturae Sinica, 2023, 50(2): 250-264.
[2]	SONG Yanhong, CHEN Yaduo, ZHANG Xiaoyu, SONG Pan, LIU Lifeng, LI Gang, ZHAO Xia, and ZHOU Houcheng, . The Transcription Factor FvbHLH130 Activates Flowering in Fragaria vesca [J]. Acta Horticulturae Sinica, 2023, 50(2): 295-306.
[3]	HAN Rui, ZHONG Xionghui, CHEN Denghui, CUI Jian, YUE Xiangqing, XIE Jianming, and KANG Jungen, . Cloning and Functional Analysis of BobHLH34 Gene in Cabbage that Interacts with XopR from Xanthomonas [J]. Acta Horticulturae Sinica, 2023, 50(2): 319-330.
[4]	TIAN Mingkang, XU Zhixiang, LIU Xiuqun, SUI Shunzhao, LI Mingyang, and LI Zhineng, . Identification of the AP2 Subfamily Transcription Factors in Chimonanthus praecox and the Functional Study of CpAP2-L11 [J]. Acta Horticulturae Sinica, 2023, 50(2): 382-396.
[5]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[6]	LIN Haijiao, LIANG Yuchen, LI Ling, MA Jun, ZHANG Lu, LAN Zhenying, YUAN Zening. Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia [J]. Acta Horticulturae Sinica, 2023, 50(1): 131-144.
[7]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YANG An, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Zhongping Youlei’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 1-2.
[8]	SUN Simiao, WANG Kun, GAO Yuan, WANG Dajiang, and LI Lianwen. A New Ornamental Crabapple Cultivar‘Zichen’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 267-268.
[9]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Pingyou 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 1-2.
[10]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Late Ripening Apple Cultivar‘Huayou Tianwa’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 3-4.
[11]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Mid-ripening Apple Cultivar‘Huayou Baomi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 5-6.
[12]	YANG Ling, CONG Peihua, WANG Qiang, LI Wuxing, and KANG Liqun. A New Mid-ripening Apple Cultivar‘Huafeng’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 7-8.
[13]	JIA Xin, ZENG Zhen, CHEN Yue, FENG Hui, LÜ Yingmin, ZHAO Shiwei. Cloning and Expression Analysis of RcDREB2A Gene in Rosa chinensis‘Old Blush’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 1945-1956.
[14]	LIU Chuanhe, HE Han, SHAO Xuehua, LAI Duo, KUANG Shizi, XIAO Weiqiang, LIU Yan. A New Pineapple Cultivar‘Yuetong’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2053-2054.
[15]	GAO Yanlong, WU Yuxia, ZHANG Zhongxing, WANG Shuangcheng, ZHANG Rui, ZHANG De, WANG Yanxiu. Bioinformatics Analysis of Apple ELO Gene Family and Its Expression Analysis Under Low Temperature Stress [J]. Acta Horticulturae Sinica, 2022, 49(8): 1621-1636.

Primary Analysis of the Function of bZIP Transcription Factor Gene MdAREB2 in Apple

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments