Abstract: The tender stems of Aconitum sungpanense were used as the explants to make a study of the adventitious bud induction and the plantlet regeneration. The results are as follows：The tender stems with the length of 5–10 cm are the ideal material for the explant culture. When the adventitious buds are inducted by the tender stems，the medium of MS + 6-BA 4.0 mg ? L-1 + NAA 1.0 mg ? L-1 is beneficial for the differentiation and the differentiation rate reaches 94.8%，besides，the number of the average bud is 4.2. The medium of MS + 6-BA 3.0 mg ? L-1 + IBA 0.3 mg ? L-1 is suitable for the propagation of buds. Meanwhile，to make the control of the vitrification of seedlings，it is effective to add Benzylpenicillin Potassium with 5 mg ? L-1 and GA3 0.3 mg ? L-1 to the original propagation medium and it is ideal to adopt sucrose with 50 g ? L-1 and agar with 7 g ? L-1 so that buds can be multiplied by 7.26 times and the height of seedlings can rise to about 4 cm as well. The optimum medium for rooting is 1/2MS + IAA 0.3 mg ? L-1 and the average number of rooting is approximately 10，besides，the rooting rate increases to over 96%. The seedlings transplanted to the matrix with forest soil and grass carbon（the ratio of 1︰1）grow vigorously and the survival rate reaches over 90%.

Key words: Aconitum sungpanense, tender stems, induction of adventitious buds, plantlet regeneration