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ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (6): 1040-1048.doi: doi:10.16420/j.issn.0513-353x.2014-1115

• Fruit Trees • Previous Articles     Next Articles

Histopathological of the Early Interactions Between Peach Roots and Meloidogyne incognita

LI Hai-Yan, WANG Li-Rong, CAO Ke, ZHU Geng-Rui, WANG Xin-Wei, FANG Wei-Chao, CHEN Chang-Wen, WANG Xiao-Li   

  1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China
  • Online:2015-06-25 Published:2015-06-25

Abstract: To study the changes in tissue structures and resistance mechanisms that occur due to the interaction between peach roots and root knot nematodes,seedlings of the resistant Baigen Gansu Tao 1 (Prunus kansuensis)and the susceptible Bailey(P. persica)were inoculated with Meloidogyne incognita using artificial indoor inoculation. Root tips were observed by staining at 6,12,18,24,30,36,42 and 48 h after inoculation,and the tissue structure of the root tips was observed by paraffin section at 0,6,12,36,60 and 84 h after inoculation. Staining results showed that the infection law of Baigen Gansu Tao 1 and Bailey were roughly similar:Root knot nematodes began invading at 12 h after inoculation,and the number of root knot nematodes increased gradually during the period between 12 h to 30 h after inoculation. The number of root knot nematodes present in the roots subsequently decreased as theorganisms began their upward movement along the plants’ vascular bundles. Compared to Bailey,at 30 h after inoculation Baigen Gansu Tao 1 were infected with root knot nematodes at a significantly lower rate:Only 28% of that of Bailey. Paraffin section results showed that the structural damage to inoculated Baigen Gansu Tao 1 was much less severe than in inoculated Bailey. Nematodes gathered in certain areas,which led to cells necrosis at the gathering sites at 84 h after inoculation.

Key words: peach, Meloidogyne incognita, root, anatomical structure, resistance mechanism

CLC Number: