Abstract: In the study，by investigating the factors influencing the transformation efficiency of Lilium
‘Siberia’，a genetic transformation system of scales was optimized. By Agrobacterium-mediated，the
AtDREB2A gene was transformed into‘Siberia’. The results indicated that the highest rate of kanamycinresistant
plants was obtained through the following transformation procedure：After preculture for 3 d，the
explants were infected for 20 min with diluted OD600 = 0.8 Agrobacterium tumefaciens solution and then
co-cultivated for 3 d（under 25 ℃）. Molecular detection showed 59 transformed lines of‘Siberia’were
detected by PCR analysis and 28 of them show the positive band same as objective band（AtDREB2A）.
The fragment was 402 bp approximately. It preliminarily suggested that the exogenous gene was integrated
into the genome of‘Siberia’. Determination of physiological indexes showed that the transgenic plants
enhanced the tolerance to high temperature.

Key words: lily, heat resistance, transcription factor, AtDREB2A, genetic transformation