Recent Advances in Brassica Genome Research by Fluorescence in situ Hybridization（FISH）

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (9): 1710-1718.

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Recent Advances in Brassica Genome Research by Fluorescence in situ Hybridization（FISH）

XUAN Shu-xin¹，FENG Da-ling²，LI Yan-bin¹，ZHAO Yu-jing¹，ZHANG Cheng-he¹，and SHEN Shu-xing^1,*

1College of Horticulture，Agricultural University of Hebei，Baoding，Heibei 071000，China；2College of Life Science，Agricultural University of Hebei，Baoding，Heibei 071000，China

Online:2013-09-25 Published:2013-09-25

Abstract

Abstract: Fluorescence in situ hybridization（FISH）is an effective and accurate method to visualize
the localization of genes and repetitive DNA sequences on chromosomes. This technique has been widely
used for physical mapping of unique nucleotide sequences on specific chromosome regions，for identifying
the specific genomes and chromosomes，for mearuring the size of various DNA molecules，and for
revealing the spatial organization of the centromere and heterochromatin or euchromatin of different
chromosomes. The present review mainly described the recent advances in Brassica genome research by
fluorescence in situ hybridization and discussed its future applications in Brassica crops.

Key words: Brassica crops, flourescence in situ hybridization（FISH）, cytogenetics, genome, chromosome painting（CCP）, extend DNA fibers（EDFs）

CLC Number:

S 63

XUAN Shu-Xin, FENG Da-Ling, LI Yan-Bin, ZHAO Yu-Jing, ZHANG Cheng-He, SHEN Shu-Xing. Recent Advances in Brassica Genome Research by Fluorescence in situ Hybridization（FISH）[J]. ACTA HORTICULTURAE SINICA, 2013, 40(9): 1710-1718.

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Recent Advances in Brassica Genome Research by Fluorescence in situ Hybridization（FISH）

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