Cloning of NAD-Malic Enzymes and Their Expression Analysis During Tissues and Fruit Development of Apple

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (4): 739-.

• Research Notes • Previous Articles Next Articles

Cloning of NAD-Malic Enzymes and Their Expression Analysis During Tissues and Fruit Development of Apple

DONG Qing-Long, YU Xian-Mei, LIU Dan-Dan, WANG Hai-Rong, AN Miao, YAO Yu-Xin, WANG Chang-Jun

（1Shandong Institute of Pomology，Tai’an，Shandong 271000，China；2College of Horticulture Science and Engineering，
Shandong Agricultural University，National Research Center for Apple Engineering and Technology，State Key Laboratory
of Crop Biology，Tai’an，Shandong 271018，China）

Online:2013-04-25 Published:2013-04-25

Abstract

Abstract: NAD-malic enzyme is a key enzyme in malic acid metabolism pathway of plants. Two
apple NAD-ME genes（MdNAD-ME1 and MdNAD-ME2）were isolated from the‘Fuji’apple leaves
（Malus × domestica Borkh.）by homologous comparison and RT-PCR confirmation. MdNAD-ME1 and
MdNAD-ME2 contained 1 785 bp and 1 818 bp ORFs，which encoded 595- and 605-aa proteins，
respectively. Amino acid sequence and structure analysis indicated that MdNAD-MEs contained five
conservative amino acid areas（Ⅰ–Ⅴ）and two functional structure domains：Malic and NAD_bind_1_
malic_enz. The result of phylogenetic analysis showed that MdNAD-ME1 belonged to dicotyledon
subgroup of α group，while MdNAD-ME2 belonged to dicotyledon subgroup of β group. qRT-PCR result
showed that MdNADP-MEs were constitutively expressed in all examined tissues，and the expression level
of MdNAD-ME1 were higher than of MdNAD-ME2 during more tissues. MdNAD-ME1 and MdNAD-ME2
had different patterns of expression during fruit development of‘Fuji’apple. Taken together，the above
results indicated that MdNAD-MEs belonged to NAD-ME family，and MdNAD-ME1 and MdNAD-ME2
might play different roles in the growth and development of apple.

Key words: apple, malic enzyme, cloning, sequence analysis, expression analysis

CLC Number:

S 661.1

DONG Qing-Long, YU Xian-Mei, LIU Dan-Dan, WANG Hai-Rong, AN Miao, YAO Yu-Xin, WANG Chang-Jun. Cloning of NAD-Malic Enzymes and Their Expression Analysis During Tissues and Fruit Development of Apple[J]. ACTA HORTICULTURAE SINICA, 2013, 40(4): 739-.

[1]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[2]	ZHAO Xueyan, WANG Qi, WANG Li, WANG Fangyuan, WANG Qing, LI Yan. Comparative Transcriptome Analysis of Differential Expression in Different Tissues of Corydalis yanhusuo [J]. Acta Horticulturae Sinica, 2023, 50(1): 177-187.
[3]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YANG An, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Zhongping Youlei’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 1-2.
[4]	SUN Simiao, WANG Kun, GAO Yuan, WANG Dajiang, and LI Lianwen. A New Ornamental Crabapple Cultivar‘Zichen’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 267-268.
[5]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Pingyou 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 1-2.
[6]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Late Ripening Apple Cultivar‘Huayou Tianwa’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 3-4.
[7]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Mid-ripening Apple Cultivar‘Huayou Baomi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 5-6.
[8]	YANG Ling, CONG Peihua, WANG Qiang, LI Wuxing, and KANG Liqun. A New Mid-ripening Apple Cultivar‘Huafeng’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 7-8.
[9]	WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe. Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate [J]. Acta Horticulturae Sinica, 2022, 49(9): 1883-1894.
[10]	LIU Chuanhe, HE Han, SHAO Xuehua, LAI Duo, KUANG Shizi, XIAO Weiqiang, LIU Yan. A New Pineapple Cultivar‘Yuetong’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2053-2054.
[11]	GAO Yanlong, WU Yuxia, ZHANG Zhongxing, WANG Shuangcheng, ZHANG Rui, ZHANG De, WANG Yanxiu. Bioinformatics Analysis of Apple ELO Gene Family and Its Expression Analysis Under Low Temperature Stress [J]. Acta Horticulturae Sinica, 2022, 49(8): 1621-1636.
[12]	LIU Chaoyang, LIAO Zhichan, LU Xinxin, HE Yehua. Identification of CslD Gene Family in Pineapple and Functional Analysis of AcoCslD2a [J]. Acta Horticulturae Sinica, 2022, 49(8): 1650-1662.
[13]	QIU Ziwen, LIU Linmin, LIN Yongsheng, LIN Xiaojie, LI Yongyu, WU Shaohua, YANG Chao. Cloning and Functional Analysis of the MbEGS Gene from Melaleuca bracteata [J]. Acta Horticulturae Sinica, 2022, 49(8): 1747-1760.
[14]	ZHENG Xiaodong, XI Xiangli, LI Yuqi, SUN Zhijuan, MA Changqing, HAN Mingsan, LI Shaoxuan, TIAN Yike, WANG Caihong. Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress [J]. Acta Horticulturae Sinica, 2022, 49(7): 1401-1414.
[15]	XIA Yan, HUANG Song, WU Xueli, LIU Yiqi, WANG Miaomiao, SONG Chunhui, BAI Tuanhui, SONG Shangwei, PANG Hongguang, JIAO Jian, ZHENG Xianbo. Identification and Analysis of Apple Viruses Diseases Based on Virome Sequencing Technology [J]. Acta Horticulturae Sinica, 2022, 49(7): 1415-1428.

Cloning of NAD-Malic Enzymes and Their Expression Analysis During Tissues and Fruit Development of Apple

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments