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ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (1): 163-168.

• Research Notes • Previous Articles     Next Articles

Response of Endogenous Jasmonates and Sesquiterpenes to Mechanical Wound in Aquilaria sinensis Stem

ZHANG  Zheng, YANG  Yun, WEI  Jian-He, MENG  Hui, WANG  Meng-Xi, HAN  Xiao-Min, SUI  Chun   

  1. (1Institute of Medicinal Plant Development,National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China;2Hainan Branch of Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences & Peking Union Medical College,Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine Wanning,Hainan 571533,China;3Drug Research Institute of Life Science and Environment Science,Harbin University of Commerce,Harbin 150076,China;4College of Environmental & Chemical Engineering, Yanshan University,Qinhuangdao,Hebei 066004, China)
  • Online:2013-01-25 Published:2013-01-25

Abstract: To confirm the function of jasmonates(Jas)in induction of sesquiterpenes,three-year-old Aquilaria sinensis(Lour.)Gilg saplings were used as materials in treatments of mechanical injury and exogenous MeJA. The contents of endogenous JAs and sesquiterpenes were detected by GC–MS,separately. The results showed that after 1 h wound treatment endogenous JAs content increased significantly,then decreased rapidly;after 24 h the contents of JAs have a slight elevation. Endogenous JAs content increased rapidly and reached a maximum at 1h,and then decreased rapidly. A second slight increase in JAs level was observed at 24 h after wound treatment. Wound induced the production of three sesquiterpenes(δ-guaiene,α-guaiene and α-humulene),and the contents increased with the time prolonged. Similarly,exogenous MeJA application also induced the production of three same sesquiterpenes,while the inducement intensity of MeJA was better than wound. It is thus suggested that JAs burst in the early-stage and sesquiterpenes biosynthesis in late-stage response to wound may constitute an important mechanism by which plant starts the related defense reaction and adapts to wound stress.

Key words: Aquilaria sinensis, jasmonates, sesquiterpenes, mechanical wound

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