Differential Proteomic Analysis of Brassica oleracea Stigma and Pollen Proteins During the Early Stage of Compatible Pollination

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (1): 69-78.

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Differential Proteomic Analysis of Brassica oleracea Stigma and Pollen Proteins During the Early Stage of Compatible Pollination

CHEN Song, ZENG Jing, GAO Qi-Guo, ZHU Li-Quan, LIU Yu-Dong, REN Xue-Song, WANG Xiao-Jia

（1College of Agronomy and Biotechnology，Southweat University，Chonqging 400716，China； 2 College of Horticulture and Landscape Architecture，Southwest University，Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education，Chongqing 400716，China）

Online:2013-01-25 Published:2013-01-25

Abstract

Abstract: In order to study the early pollen-stigma interaction in Brassica oleracea through proteomics analysis，inbred line A₄ was pollinated with inbred line K₁₀₁. Total stigma/pollen proteins were extracted by TCA/acetone at 3–5 min，1 h and 2 h after pollination，respectively. Total proteins were separated by two-dimensional gel electrophoresis and analyzed through MALDI-TOF-TOF-MS mass spectrometry. 116 proteins were differentially expressed contrasted the total stigma/pollen proteins of 1 h and 3–5 min after pollination，but only 7 proteins expressed differentially contrasted the total stigma/pollen proteins of 1 h and 2 h after pollination. 71 protein spots were analyzed through MALDI-TOF-TOF-MS mass spectrometry，and 31 proteins were identified homologous to known proteins by MASCOT analysis. Compared with total stigma/ pollen proteins of 3–5 min after pollination，19 of 31 identified proteins were specifically expressed 1 h after pollination，9 up-regulated and 3 down-regulated ones. The predicted functions of the 31 protein were related to pollen tube development，Ca²⁺ binding，biosynthetic process，ransmembrane transport，defense response，protein folding，carbohydrate and energy metabolism，regulation of translation and methylation. This study has documented the dynamics of protein expression during the early pollen-stigma interaction in Brassica oleracea and provides insights into the fundamental mechanisms involved in these processes.

Key words: Brassica oleracea L., pollination, pollen-stigma interaction, proteomics, 2-DE

CLC Number:

S635

CHEN Song, ZENG Jing, GAO Qi-Guo, ZHU Li-Quan, LIU Yu-Dong, REN Xue-Song, WANG Xiao-Jia. Differential Proteomic Analysis of Brassica oleracea Stigma and Pollen Proteins During the Early Stage of Compatible Pollination[J]. ACTA HORTICULTURAE SINICA, 2013, 40(1): 69-78.

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Differential Proteomic Analysis of Brassica oleracea Stigma and Pollen Proteins During the Early Stage of Compatible Pollination

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