Abstract: In this study, the effects of different concentrations of CoCl₂, ABA, sucrose and the condition of illumination on the status of embryogenic callus that induced from pseudo-bulblet of Lilium longiflorum was studied，and the regeneration of embryogenic callus was studied from active carbon. The results showed that the proportion of somatic embryos and granularity of the callus could be increased by using CoCl₂ with the concentration of 0.05 μmol·L^-1, while 0.01 μmol·L^-1 and 0.02 μmol·L^-1 CoCl₂ are beneficial to the increasement of callus volume and proliferation coefficient; Some negative effect on growth potential, water content, volume and proliferation coefficient of embryogenic callus was observed when the concentration of ABA was gradually increased, while proportion and granularity of embryogenic callus were not affected by different ABA concentrations; Culturing in light, the positive effects of 6.0 g·L^-1 or 9.0 g·L^-1 sucrose on proportion and granularity of embryogenic callus were observed, while only 6.0 g·L^-1 sucrose has positive effects when cultured at dark; Furthermore, the regeneration of embryogenic callus was affected by 1g/L active carbon and basic medium. Considering all factors in present study, the results demonstrated that the most appropriate condition for the proliferation and regeneration of embryogenic callus were: culture under light, MS + NAA(5.4μM/L) + TDZ(0.4μM/L) + CoC12(0.05μM/L) + source(60g/L) and MS+ active carbon (1g/L) + source(30g/L)。

Key words: Lilium longiflorum, embryogenic callus, somatic embryo, adjustment, regeneration