Detection and Sequencing of Apple scar skid viroid from Apricot in Xinjiang

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (6): 805-810.

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Detection and Sequencing of Apple scar skid viroid from Apricot in Xinjiang

ZHAO Ying and NIU Jian-xin*

(Department of Horticulture, Agricultural College of Shihezi University, Shihezi,Xinjiang832003，China)

Received:2008-03-07 Revised:2008-05-13 Online:2008-06-25 Published:2008-06-25
Contact: NIU Jian-xin

Abstract

Abstract:

Present study was conducted based on the previous research of Apple scar skin viroid (ASSVd) for apple and pear, used material of apricot was those that showed positive signal of blot hybridization detection. Full-length special fragment of ASSVd for apricot was amplicated using RT-PCR protocol, then was recycled, cloned and sequenced. It was demonstrated that the full-length genome of ASSVd of apricot is 330 nt long (GenBank numbered with EU031477 to EU031486). The exist of ASSVd in apricots was reconfirmed by the results of In situ RT - PCR , and ASSVd mainly located in nucleolus of mesophyll cell. The fact of infecting capability of apricot enlarged the host ranges of ASSVd.

Key words: apricot, Apple scar skid viroid, blot hybridization, RT-PCR, situ RT-PCR, full length analysis

CLC Number:

S 662.2

ZHAO Ying and NIU Jian-xin. Detection and Sequencing of Apple scar skid viroid from Apricot in Xinjiang [J]. ACTA HORTICULTURAE SINICA, 2008, 35(6): 805-810.

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Detection and Sequencing of Apple scar skid viroid from Apricot in Xinjiang

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