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ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (5): 881-884.

• 研究报告 • Previous Articles     Next Articles

Full-length cDNA Clone and Sequences Analysis for Unknown Double-stranded RNA Isolated from Radish

Liu Li1;Chen Jishuang2;Yu Shan3;Wang Chong3   

  1. (1 Faculty of Life Sciences, Huzhou Teachers College, Huzhou 313000, China; 2 Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; 3College of Life Sciences, Zhejiang University, Hangzhou 310029, China)
  • Received:2005-02-15 Revised:2005-05-15 Online:2005-10-25 Published:2005-10-25

Abstract: Some characters of unknown dsRNAs isolated from radish were identified. The dsRNAs could be detected from some radish plants and their offspring by dsRNAs analysis approach. The dsRNAswere separated into two fragments in polyacrylamide gel, named dsRNA1 and dsRNA2, respectively with their sizes of
approximately 118 kbp. Full-length cDNA of dsRNA1 was obtained by single primer amplification technique, and its size of 1 866 ntwas determined. RT-PCR also showed that dsRNA1 was of radish plant origin. The results of BLASTP suggested that the biggest putative ORF1 was distantly related to RNA dependent RNA polymerase encoded by some species of Partitivirus. The molecular aspects of dsRNAs, and some biological characters of plants, seem related to radish yellow edge virus (RYEV) previously described.

Key words: Radish, Double-stranded RNA, cDNA cloning, Sequence, Radish yellow edge virus

CLC Number: