Abstract: The resistant test of Volvariella volvacea strain V1308 to hygromycin, kanamycin, G418 and PPT showed that the strain was sensitive to the former but resistant to the latter 3 reagents. The lowest selection concentrations of hygromycin for V1308 were 75μg·mL ^{- 1} on PDSA solid medium and 50μg·mL ^{- 1} in PDSB liquid medium. The expression vector pCAMB IA1301, carrying 35S promoter, hygromycin selectable gene and gus report gene, was transferred into intactmycelia of V1308 by particle bombardment. The most effective bombardment parameters were 1 100 Psi of helium pressure, 87188 kPa of vacuum pressure and 6 cm of target-shelf distance. The result of southern blotting indicated that the gus gene was integrated into the V1308 genome. GUS histochemical assay confirmed that gus had exp ressed in V1308 mycelia. Cultivation of V1308 showed that both the transformant and the control were able to form normal fruit-bodies. GUS histochemical assay indicated that the exogenous gus gene expressed stably in the mycelia derived from the fruit-bodies of transformant.

Key words: Volvariella volvacea, Particle bombardment, Genetic transformation, gus gene