Cloning and Expression Analysis of Banana ACC Oxidase Gene (MAO3)

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (5): 807-811.

• 研究论文 • Previous Articles Next Articles

Cloning and Expression Analysis of Banana ACC Oxidase Gene (MAO3)

Huang Junsheng;Wang Hua;Zhang Shiqing

(¹ Enviroment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China;²State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

Received:2004-12-01 Revised:2005-02-02 Online:2005-10-25 Published:2005-10-25

Abstract

Abstract: We obtained core sequence of MAO3 of banana according to homo-cloning, and then cloned 5'up stream and 3'down stream sequences by RACE PCR technology and the promoter sequence by GenomeWalker technology. Phylogenetic analysis showed that this gene was highly conserved in the same species and had comparatively high homology compared with both monocots and dicots. In situ hybridization experiment showed that the expression of MAO3 gene was tissue specific. Meanwhile, according to fluorescence real-time
quantification PCR, we found that MAO3 and ERS2 (one of banana ethylene receptor genes) genes were also induced by wounding.

Key words: Banana, MAO3 gene, Cloning, Homology analysis, Tissue specific expression analysis, Wounding induce

CLC Number:

S668.1

Huang Junsheng;Wang Hua;Zhang Shiqing. Cloning and Expression Analysis of Banana ACC Oxidase Gene (MAO3)[J]. ACTA HORTICULTURAE SINICA, 2005, 32(5): 807-811.

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Cloning and Expression Analysis of Banana ACC Oxidase Gene (MAO3)

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