Abstract: In order to get the optimum photoperiod parameter for the production of bulblet by tissue culture，the experiment was conducted with 5 photoperiod treatments (darkness time per day：0，8，12，16，24 h) to investigate the effect of photoperiods on the bulblet morphogenesis，amylase activity，as well as the utilization rate of sucrose in the culture medium absorbed by bulblet of the wild Lilium lancifolium Thunb. in vitro. The results showed that the different periods of darkness had a significant effect on the organ formation (root，leaf and bulblet) and the weight of bulblets in test tube，the treatment 16 h · d-1 darkness had better effect for the bulblet formation and enlargement. There were differences between total soluble sugar content，starch content and the amylase activity in bulblets with different photoperiod treatments. After 60 days culture in the test tube，the starch content in bulblets with treatments of 12，16，24 h · d-1 darkness was higher than that with 0，8 h · d-1. The utilization rate of sucrose in the culture medium for bublets was also different among all treatments；The 16 h · d-1 darkness was optimum photoperiodtreatment for bulblet production with tissue culture technology.

Key words: lily, wild Lilium lancifolium Thunb., bulblet in vitro, photoperiod