Detection of Lily symptomless virus and Lily mottle virus by Multiplex RT-PCR

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (02): 284-287.

• 研究论文 • Previous Articles Next Articles

Detection of Lily symptomless virus and Lily mottle virus by Multiplex RT-PCR

Wang Jihua¹;Wang Lihua¹;Ding Yuanming²;Qu Suping¹;Xiong Li¹;Tang Kaixue¹

(¹ Yunnan Agriculture Academy, Supervision and Testing Centre for Flower of Agriculture Ministry, Kunming 650205, China;² Yunnan Entry-exit Inspection & Quarantine Bureau, Kunming 650228, China)

Received:2004-07-14 Revised:2004-11-01 Online:2005-04-25 Published:2005-04-25
Contact: Tang Kaixue

Abstract

Abstract: Two sets of specific primers were designed according to LSV and LM oV CP gene sequence,and the conditions for PCR were optimized. The multiplex PCR method which can simultaneously detect the two lily viruses was developed. It was showed that all samples which infected LSV and LMoV could be amplified by multiplex PCR, yielding two specific bands of LSV (876 bp) and LMoV (662 bp). The two viruses were detected from dilutions of 10⁴ by multiplex RT-PCR and RT-PCR, respectively. Sequence analysis on the amplified products show that the nucleotide sequences homology of LSV was 87.8% -99.3% compared with
sequences of other isolates, and LMoV was 90.1%-99.5%.

Key words: Multiplex PCR, Detection, Lily symptomless virus, Lily mottle virus

CLC Number:

S

S682.2

Wang Jihua;Wang Lihua;Ding Yuanming;Qu Suping;Xiong Li;Tang Kaixue. Detection of Lily symptomless virus and Lily mottle virus by Multiplex RT-PCR[J]. ACTA HORTICULTURAE SINICA, 2005, 32(02): 284-287.

[1]	CHEN Lilang, YANG Tianzhang, CAI Ruping, LIN Xiaoman, DENG Nankang, CHE Haiyan, LIN Yating, KONG Xiangyi. Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan [J]. Acta Horticulturae Sinica, 2022, 49(8): 1785-1794.
[2]	XIA Yan, HUANG Song, WU Xueli, LIU Yiqi, WANG Miaomiao, SONG Chunhui, BAI Tuanhui, SONG Shangwei, PANG Hongguang, JIAO Jian, ZHENG Xianbo. Identification and Analysis of Apple Viruses Diseases Based on Virome Sequencing Technology [J]. Acta Horticulturae Sinica, 2022, 49(7): 1415-1428.
[3]	GONG Mingxia, ZHAO Hu, WANG Meng, WU Xing, ZHAO Zengjing, HE Zhi, HUANG Jinmei, MENG Shengde, WANG Risheng. Identification of Viruses Infecting Peppers in Guangxi by Small RNA Deep Sequencing and RT-PCR [J]. Acta Horticulturae Sinica, 2022, 49(5): 1060-1072.
[4]	JIA Jin, XU Yunlong, ZHOU Jiale, YANG Xiaofen, ZHONG Yonghui, ZENG Jiwu, HONG Ni, WANG Guoping, PENG Shu’ang, DING Fang. Detection and Identification of Huanglongbing Bacteria in‘Cocktail’Grapefruit [J]. Acta Horticulturae Sinica, 2022, 49(3): 590-596.
[5]	CHEN Jianxin, WEI Yuqian, TANG Jie, ZHU Yongjin, MA Huancheng, WU Jianrong. Molecular Detection and Sequence Analysis of ORSV and CymMV in Cultivated Cymbidium goeringii in Dali,Yunnan [J]. Acta Horticulturae Sinica, 2022, 49(3): 655-662.
[6]	LÜ Yan, DUAN Weijun. TaqMan MGB-based Real-time Fluorescence PCR Method for the Rapid Detection of Ciborinia camelliae [J]. Acta Horticulturae Sinica, 2022, 49(3): 663-670.
[7]	MENG Zhen, ZHANG Weiping, WANG Ying, LI Long, JI Xiaoxue, DONG Bei, QIAO Kang. Establishment and Application of Real-Time PCR for Quantitative Detection of Fusarium oxysporum f. sp. lycopersici [J]. Acta Horticulturae Sinica, 2022, 49(11): 2479-2488.
[8]	FENG Lixiao, HU Rong, BU Shan, ZHANG Deyong, LUO Xiangwen, LI Fan, DING Ming, ZHANG Zhuo, ZHANG Songbai, LIU Yong. Molecular Detection and Genetic Evolution Analysis of Yunnan Isolates of Lettuce Chlorosis Virus in Tomato [J]. Acta Horticulturae Sinica, 2022, 49(1): 141-147.
[9]	MA Zhimin, DUAN Yu, XU Jianjian, BIN Yu, ZHOU Changyong, SONG Zhen. The Rapid Detection of Xanthomonas citri ssp. citri（Xcc）Based on Recombinase Polymerase Amplification（RPA）Assay [J]. Acta Horticulturae Sinica, 2021, 48(3): 590-599.
[10]	SONG Yuhao, SHI Weiling, ZHANG Jiao, DING Zhenyu, PENG Hongxian, JIANG Chunyan, MA Qiuqin, LI Zhijing, ZHAO Yong, TANG Daobin, ZHANG Kai, WANG Jichun, LIU Xun. Development and Application of an Efficient Method for the Amylose/ Amylopectin Ratio Determination in Potato Tubers [J]. Acta Horticulturae Sinica, 2021, 48(3): 600-608.
[11]	FAN Xudong, DONG Yafeng, ZHANG Zunping, REN Fang, HU Guojun, ZHANG Mengyan, LI Chen. Detection and Sequence Analyses of Grapevine Leafroll-associated Virus 7 Isolates in China [J]. Acta Horticulturae Sinica, 2021, 48(2): 347-354.
[12]	LIU Cheng, WANG Shiyao, SHI Weiling, SONG Yuhao, JIANG Rui, ZHAO Yong, MO Shichun, LÜ Dianqiu, WANG Jichun, LIU Xun. Multiplex PCR Detection for Late Blight Resistant Genes R8,RB and Virus Resistant Genes Rx1,Ry_adg in Potato [J]. Acta Horticulturae Sinica, 2021, 48(2): 389-396.
[13]	CHEN Ling, YAN Guohua, ZHANG Xiaoming, ZHOU Yu, WANG Jing, DUAN Xuwei, LI Yanlin, ZHANG Kaichun. Establishment of Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Detection of Prune Dwarf Virus [J]. Acta Horticulturae Sinica, 2021, 48(1): 183-192.
[14]	ZHAO Lei1，ZHANG Yuanle1，HUANG Qianru1，HOU Caiting1，WU Kuan1,3，CAO Mengji4，WANG Qiaochun1,2,，and WU Yunfeng1,. Distribution，Molecular Characterization and Genome Sequence of Actinidia virus A（AcVA）and Actinidia virus B（AcVB）in Shaanxi [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1383-1390.
[15]	WU Shuhua1,，TU Liqin1,，XIAN Wenrong2，YAN Jiahui2，GAO Danna1，JI Ying2，TAO Xiaorong3，ZHOU Yijun1，GUO Qingyun2,，and JI Yinghua1,. Detection and Identification of Tomato spotted wilt tospovirus on Pepper from Qinghai Province [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1391-1400.

Detection of Lily symptomless virus and Lily mottle virus by Multiplex RT-PCR

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments