Development of cDNA Dimeric Probes and Its Application in Diagnosis ofPotato spindle tuber viroid

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (10): 1531-1537.

• Technologies and Methods • Previous Articles Next Articles

Development of cDNA Dimeric Probes and Its Application in Diagnosis ofPotato spindle tuber viroid

LüDian-qiu*; QIU Cai-ling;WANG Shao-peng;LI Yong;GAO Yun-fei

(Viruses-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Supervision and Testing Center for Virus-free Potato Seeds of Ministry of Agriculture,Harbin 150086, China)

Received:2009-06-12 Revised:2009-08-19 Online:2009-10-25 Published:2009-10-25
Contact: LüDian-qiu

Abstract

Abstract: Two PCR amplifying full-length PSTVd (Potato spindle tuber viroid) with the B amHⅠ (87-92 nt) overlop were linked to pGM-T to construct the recombinant vector containing dimeric PSTVd cDNA. Highly sensitive and specific dimeric probe labeled with digoxin was produced with PCR amplification. An efficient hybridization system was established bywith the substrate of CDP-star in this study. The minimum concentration of PSTVd detected by this probe was 0.05 pg. The samples prepared from leaves, tubers and sprouts could be detected successfully. Compared the positive sample percentage detected from potato fields and conserved potato resource of plantlets in vitro with RT-PCR ( reverse-transcrip tional polymerase chain reaction) and R-PAGE ( return-polyacrylamide gel electrophoresis) , NASH ( nucleic acid spot hybridization)
could give the same positive sample percentage as RT-PCR did ( 67.7% ) , but was higher than R-PAGE (53.7% ).

Key words: potato, Potato spindle tuber viroid, PSTVd, probe, dimeric PSTVd, nucleic acid spot hybridization (NASH)

CLC Number:

S 532

LüDian-qiu;QIU Cai-ling;WANG Shao-peng;LI Yong;GAO Yun-fei. Development of cDNA Dimeric Probes and Its Application in Diagnosis ofPotato spindle tuber viroid[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1531-1537.

[1]	WANG Kuan, QI Lipan, WU Guili, FENG Yan, WANG Lei, YIN Jiang, LUO Yating, WANG Yan, LIU Chang, GONG Xuechen, and WANG Haijun. A New Potato Cultivar‘Beifang 002’of Early Maturity and Good Yield [J]. Acta Horticulturae Sinica, 2022, 49(S2): 141-142.
[2]	QI Lipan, FENG Yan, WANG Lei, YIN Jiang, WANG Kuan, LUO Yating, GONG Xuechen, LIU Chang, and WANG Yan. A New Potato Cultivar‘Beifang 004’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 143-144.
[3]	LI Yanshan, SUI Qijun, JIANG Wei, YANG Qiongfen, and BAI Jianming. A New Potato Cultivar‘Yunshu 113’for Both Fresh Consumption and Starch Extraction [J]. Acta Horticulturae Sinica, 2022, 49(S2): 145-146.
[4]	ZOU Xue, DING Fan, LIU Lifang, YU Hankaizong, CHEN Nianwei, and RAO Liping. A New Purple Potato Cultivar‘Mianziyu 1’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 93-94.
[5]	YAN Wenyuan, QIN Junhong, DUAN Shaoguang, XU Jianfei, JIAN Yinqiao, JIN Liping, LI Guangcun. The Effect of Water-nitrogen Coupling on Potato Photosynthesis,Tuber Formation and Quality [J]. Acta Horticulturae Sinica, 2022, 49(7): 1491-1504.
[6]	QI Lipan, LI Yue, WANG Lei, FENG Yan, WANG Kuan, YIN Jiang, GUO Huachun. Anatomical Observation on the Graft Union Between Potato and Wolfberry [J]. Acta Horticulturae Sinica, 2022, 49(4): 868-874.
[7]	LÜ Yan, DUAN Weijun. TaqMan MGB-based Real-time Fluorescence PCR Method for the Rapid Detection of Ciborinia camelliae [J]. Acta Horticulturae Sinica, 2022, 49(3): 663-670.
[8]	CHEN Jingyi, FANG Boping, ZHANG Xiongjian, HUANG Lifei, WANG Zhangying, and LUO Zhongxia. A New Leaf-vegetable Sweet Potato Cultivar‘Guangcaishu 5’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2861-2862.
[9]	LUO Hongyu, YANG Jiangwei, FENG Ya, ZHANG Huanhuan, LIU Shengyan, ZHANG Ning, SI Huaijun. The Effect of Stu-miR156 Silencing by STTM Technology on Potato Lateral Root Development [J]. Acta Horticulturae Sinica, 2021, 48(3): 531-538.
[10]	SONG Yuhao, SHI Weiling, ZHANG Jiao, DING Zhenyu, PENG Hongxian, JIANG Chunyan, MA Qiuqin, LI Zhijing, ZHAO Yong, TANG Daobin, ZHANG Kai, WANG Jichun, LIU Xun. Development and Application of an Efficient Method for the Amylose/ Amylopectin Ratio Determination in Potato Tubers [J]. Acta Horticulturae Sinica, 2021, 48(3): 600-608.
[11]	LIU Cheng, WANG Shiyao, SHI Weiling, SONG Yuhao, JIANG Rui, ZHAO Yong, MO Shichun, LÜ Dianqiu, WANG Jichun, LIU Xun. Multiplex PCR Detection for Late Blight Resistant Genes R8,RB and Virus Resistant Genes Rx1,Ry_adg in Potato [J]. Acta Horticulturae Sinica, 2021, 48(2): 389-396.
[12]	XU Guochun, LUO Wenbin, LI Huawei, XU Yongqing, JI Rongchang, ZHANG Hong, QIU Sixin, TANG Hao. Screening for Genetic Variation in Photosynthesis and High Photosynthetic Efficiency Germplasm in Potato [J]. Acta Horticulturae Sinica, 2021, 48(11): 2239-2250.
[13]	LI Peihua, PENG Zhengsong, XIA Mingzhong, CAI Guangze, YUAN Ying, FANG Zhirong, QU Jipeng, QING Yuan. Adaptable,High Yield and Disease Resistant Potato Cultivar‘Xiyu 3’ [J]. Acta Horticulturae Sinica, 2021, 48(10): 2115-2116.
[14]	LAN Mengjiao, WU Wensheng, XIAO Manqiu, PAN Hao, HOU Longying, GE Ruihua. A New Sweet Potato Cultivar‘Ganshu 4’ [J]. Acta Horticulturae Sinica, 2021, 48(10): 2117-2126.
[15]	JIAN Hongju, WAN Mengyuan, FU Yi, DING Yi, JU Xisan, SHANG Lina, LI Hui, WANG Jichun, HU Bogeng, LÜ Dianqiu. Genome-wide Identification and Expression Analysis of Chitinase Gene Family in Potato and Its Response to Exogenous Salicylic Acid and Jasmonic Acid [J]. Acta Horticulturae Sinica, 2021, 48(1): 83-95.

Development of cDNA Dimeric Probes and Its Application in Diagnosis ofPotato spindle tuber viroid

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments