Abstract: The nuclear encoded internal transcribed spacer(ITS) region and the plastid encoded trnL-F
region were sequenced for 17 samples from 12 species of the genus Ardisia Sw. and outgroup Rapanea lin-
earis. Based on maximum parsim ony and neighbor-joining analyses, we reconstructed the phylogenetic rela-
tionships of 19 samples of Ardisia. The two methods generated similar trees. Nineteen samples of Ardisia are
separated into six clades intrnL-F trees. Eighteen samples of Ardisia are separated into two clades in ITS
trees. The results showed that the genus Ardisia Sw. could not be amonophyletic group. The Sec.t  Akosmos
(Ñ) and Sec.t Bladhia (Ó) form a clade in ITS trees, which indicated that they were closely related.
Four samples of A. japonica form two clades in ITS trees, which showed that there were at least two types of
nuclear genome in A. japonica population. According to analysis of the chromosomes number, ITS sequences
and trnL-F sequences, A. japonicais supposed to be a allotetraploid.

Key words: Ardisia, ITS sequence;trnL-F sequence, interspecific relationship