Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 2089-2104.doi: 10.16420/j.issn.0513-353x.2023-0604
• Cultivation · Physiology & Biochemistry • Previous Articles Next Articles
ZHANG Fan, YANG Yang, WANG Hong*(), ZHANG Xuebing
Received:
2024-05-21
Revised:
2024-07-02
Online:
2024-09-25
Published:
2024-09-19
Contact:
WANG Hong
ZHANG Fan, YANG Yang, WANG Hong, ZHANG Xuebing. Improving Physicochemical Properties and Bacterial Communities of Peach-Heavy Soils Using Biofertilizers and Organic Fertilizers[J]. Acta Horticulturae Sinica, 2024, 51(9): 2089-2104.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0604
处理 Treatment | 主要成分及含量 Main ingredient content | 施用量/(kg · m-2) Application rate |
---|---|---|
生物有机肥 Bio-organic fertiliser | 有效活菌数(枯草芽孢杆菌、地衣芽孢杆菌)≥ 0.2 × 108 · g-1,有机质 ≥ 40.0% Effective number of live bacteria(bacillus subtilis,bacillus licheniformis)≥ 0.2 × 108 · g-1,organic matter ≥ 40.0% | 5 |
硫酸亚铁 Ferrous sulfate | FeSO4 ≥ 99.0% | 0.5 |
松土精 Grondverbeteraar | 聚丙烯酰胺 ≥ 99.0% Polyacrylamide ≥ 99.0% | 0.025 |
枯草芽孢杆菌 Bacillus | 枯草芽孢杆菌 ≥ 400 × 108 · g-1 Bacillus subtilis ≥ 400 × 108 · g-1 | 0.02 |
农家肥(牛粪︰羊粪 = 2︰1,完全腐熟) Farmyard manure(cow dung︰sheep dung 2︰1,fully rotted) | 有机质17.67%,全氮0.53%,全磷0.37%,全钾0.13% Organic matter 17.67%,Total N 0.53%,Total P 0.37%,Total K 0.13% | 5 |
Table 1 Detailed information on soil application of fertilisers or reagent processes
处理 Treatment | 主要成分及含量 Main ingredient content | 施用量/(kg · m-2) Application rate |
---|---|---|
生物有机肥 Bio-organic fertiliser | 有效活菌数(枯草芽孢杆菌、地衣芽孢杆菌)≥ 0.2 × 108 · g-1,有机质 ≥ 40.0% Effective number of live bacteria(bacillus subtilis,bacillus licheniformis)≥ 0.2 × 108 · g-1,organic matter ≥ 40.0% | 5 |
硫酸亚铁 Ferrous sulfate | FeSO4 ≥ 99.0% | 0.5 |
松土精 Grondverbeteraar | 聚丙烯酰胺 ≥ 99.0% Polyacrylamide ≥ 99.0% | 0.025 |
枯草芽孢杆菌 Bacillus | 枯草芽孢杆菌 ≥ 400 × 108 · g-1 Bacillus subtilis ≥ 400 × 108 · g-1 | 0.02 |
农家肥(牛粪︰羊粪 = 2︰1,完全腐熟) Farmyard manure(cow dung︰sheep dung 2︰1,fully rotted) | 有机质17.67%,全氮0.53%,全磷0.37%,全钾0.13% Organic matter 17.67%,Total N 0.53%,Total P 0.37%,Total K 0.13% | 5 |
处理 Treatment | pH | 全盐量/% Total salt content | 电导率/ (mS · cm-1) EC | g · kg-1 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
全氮 Total N | 全磷 Total P | 全钾 Total K | 有机质 Organic matter | ||||||||||
对照Control | 8.61 ± 0.02 a | 0.07 ± 0.28 c | 0.28 ± 0.02 d | 1.19 ± 0.03 d | 0.85 ± 0.05 d | 16.85 ± 0.02 d | 19.82 ± 0.01 d | ||||||
生物有机肥 Bio-organic fertilizer | 7.65 ± 0.03 e | 0.13 ± 0.01 a | 0.53 ± 0.02 a | 1.93 ± 0.02 a | 1.35 ± 0.02 a | 18.40 ± 0.04 a | 28.60 ± 0.02 a | ||||||
硫酸亚铁 Ferrous sulfate | 7.95 ± 0.01 d | 0.12 ± 0.02 b | 0.49 ± 0.02 a | 1.33 ± 0.04 c | 1.04 ± 0.02 b | 17.95 ± 0.03 b | 21.73 ± 0.03 c | ||||||
松土精 Grondverbeteraar | 8.01 ± 0.02 c | 0.10 ± 0.01 b | 0.41 ± 0.01 b | 1.18 ± 0.02 d | 0.98 ± 0.01 c | 18.00 ± 0.03 b | 18.12 ± 0.02 f | ||||||
枯草芽孢杆菌 Bacillus | 8.16 ± 0.01 b | 0.09 ± 0.01 b | 0.35 ± 0.01 c | 1.54 ± 0.02 b | 1.01 ± 0.02 bc | 16.91 ± 0.02 d | 18.41 ± 0.01 e | ||||||
农家肥 Farmyard manure | 8.16 ± 0.01 b | 0.09 ± 0.01 b | 0.34 ± 0.02 c | 1.33 ± 0.01 c | 1.04 ± 0.02 b | 17.81 ± 0.01 c | 23.52 ± 0.03 b | ||||||
处理 Treatment | mg · kg-1 | ||||||||||||
碱解氮 Alkali-hydrolyzale N | 有效磷 Available P | 速效钾 Available K | 有效锌 Available Zn | 有效铁 Available Fe | |||||||||
对照Control | 116.08 ± 0.03 f | 24.32 ± 0.03 e | 144.00 ± 1.00 d | 3.46 ± 0.02 d | 9.41 ± 0.01 f | ||||||||
生物有机肥 Bio-organic fertilizer | 224.00 ± 0.02 a | 43.82 ± 0.02 a | 279.33 ± 1.53 a | 6.73 ± 0.04 a | 12.40 ± 0.01 a | ||||||||
硫酸亚铁 Ferrous sulfate | 150.10 ± 0.03 c | 28.82 ± 0.03 c | 160.67 ± 1.15 c | 3.16 ± 0.02 e | 11.89 ± 0.02 b | ||||||||
松土精 Grondverbeteraar | 132.23 ± 0.03 d | 23.78 ± 0.03 f | 115.00 ± 1.00 e | 2.85 ± 0.01 f | 9.61 ± 0.01 e | ||||||||
枯草芽孢杆菌 Bacillus | 164.11 ± 0.03 b | 24.44 ± 0.03 d | 114.00 ± 1.73 e | 3.82 ± 0.84 c | 9.82 ± 0.02 d | ||||||||
农家肥 Farmyard manure | 124.34 ± 0.03 e | 35.13 ± 0.03 b | 258.67 ± 2.08 b | 4.29 ± 0.01 b | 10.21 ± 0.02 c | ||||||||
处理 Treatment | g · kg-1 | ||||||||||||
K+ | Na+ | Ca2+ | Mg2+ | Cl- | |||||||||
对照Control | 0.07 ± 0.00 d | 0.10 ± 0.01 a | 0.16 ± 0.01 d | 0.04 ± 0.01 c | 0.14 ± 0.01 b | ||||||||
生物有机肥 Bio-organic fertilizer | 0.12 ± 0.01 a | 0.09 ± 0.01 ab | 0.29 ± 0.01 b | 0.06 ± 0.02 a | 0.26 ± 0.01 a | ||||||||
硫酸亚铁 Ferrous sulfate | 0.08 ± 0.02 c | 0.06 ± 0.00 c | 0.32 ± 0.01 a | 0.07 ± 0.01 a | 0.12 ± 0.01 b | ||||||||
松土精 Grondverbeteraar | 0.07 ± 0.00 d | 0.07 ± 0.01 b | 0.24 ± 0.01 c | 0.06 ± 0.01 b | 0.05 ± 0.03 c | ||||||||
枯草芽孢杆菌 Bacillus | 0.07 ± 0.00 d | 0.07 ± 0.01 b | 0.22 ± 0.02 d | 0.05 ± 0.01 b | 0.06 ± 0.01 c | ||||||||
农家肥 Farmyard manure | 0.10 ± 0.02 b | 0.09 ± 0.02 ab | 0.18 ± 0.0 1d | 0.05 ± 0.01 b | 0.25 ± 0.02 a |
Table 2 Effects of different improvement measures on physicochemical property of continuous cropping soil
处理 Treatment | pH | 全盐量/% Total salt content | 电导率/ (mS · cm-1) EC | g · kg-1 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
全氮 Total N | 全磷 Total P | 全钾 Total K | 有机质 Organic matter | ||||||||||
对照Control | 8.61 ± 0.02 a | 0.07 ± 0.28 c | 0.28 ± 0.02 d | 1.19 ± 0.03 d | 0.85 ± 0.05 d | 16.85 ± 0.02 d | 19.82 ± 0.01 d | ||||||
生物有机肥 Bio-organic fertilizer | 7.65 ± 0.03 e | 0.13 ± 0.01 a | 0.53 ± 0.02 a | 1.93 ± 0.02 a | 1.35 ± 0.02 a | 18.40 ± 0.04 a | 28.60 ± 0.02 a | ||||||
硫酸亚铁 Ferrous sulfate | 7.95 ± 0.01 d | 0.12 ± 0.02 b | 0.49 ± 0.02 a | 1.33 ± 0.04 c | 1.04 ± 0.02 b | 17.95 ± 0.03 b | 21.73 ± 0.03 c | ||||||
松土精 Grondverbeteraar | 8.01 ± 0.02 c | 0.10 ± 0.01 b | 0.41 ± 0.01 b | 1.18 ± 0.02 d | 0.98 ± 0.01 c | 18.00 ± 0.03 b | 18.12 ± 0.02 f | ||||||
枯草芽孢杆菌 Bacillus | 8.16 ± 0.01 b | 0.09 ± 0.01 b | 0.35 ± 0.01 c | 1.54 ± 0.02 b | 1.01 ± 0.02 bc | 16.91 ± 0.02 d | 18.41 ± 0.01 e | ||||||
农家肥 Farmyard manure | 8.16 ± 0.01 b | 0.09 ± 0.01 b | 0.34 ± 0.02 c | 1.33 ± 0.01 c | 1.04 ± 0.02 b | 17.81 ± 0.01 c | 23.52 ± 0.03 b | ||||||
处理 Treatment | mg · kg-1 | ||||||||||||
碱解氮 Alkali-hydrolyzale N | 有效磷 Available P | 速效钾 Available K | 有效锌 Available Zn | 有效铁 Available Fe | |||||||||
对照Control | 116.08 ± 0.03 f | 24.32 ± 0.03 e | 144.00 ± 1.00 d | 3.46 ± 0.02 d | 9.41 ± 0.01 f | ||||||||
生物有机肥 Bio-organic fertilizer | 224.00 ± 0.02 a | 43.82 ± 0.02 a | 279.33 ± 1.53 a | 6.73 ± 0.04 a | 12.40 ± 0.01 a | ||||||||
硫酸亚铁 Ferrous sulfate | 150.10 ± 0.03 c | 28.82 ± 0.03 c | 160.67 ± 1.15 c | 3.16 ± 0.02 e | 11.89 ± 0.02 b | ||||||||
松土精 Grondverbeteraar | 132.23 ± 0.03 d | 23.78 ± 0.03 f | 115.00 ± 1.00 e | 2.85 ± 0.01 f | 9.61 ± 0.01 e | ||||||||
枯草芽孢杆菌 Bacillus | 164.11 ± 0.03 b | 24.44 ± 0.03 d | 114.00 ± 1.73 e | 3.82 ± 0.84 c | 9.82 ± 0.02 d | ||||||||
农家肥 Farmyard manure | 124.34 ± 0.03 e | 35.13 ± 0.03 b | 258.67 ± 2.08 b | 4.29 ± 0.01 b | 10.21 ± 0.02 c | ||||||||
处理 Treatment | g · kg-1 | ||||||||||||
K+ | Na+ | Ca2+ | Mg2+ | Cl- | |||||||||
对照Control | 0.07 ± 0.00 d | 0.10 ± 0.01 a | 0.16 ± 0.01 d | 0.04 ± 0.01 c | 0.14 ± 0.01 b | ||||||||
生物有机肥 Bio-organic fertilizer | 0.12 ± 0.01 a | 0.09 ± 0.01 ab | 0.29 ± 0.01 b | 0.06 ± 0.02 a | 0.26 ± 0.01 a | ||||||||
硫酸亚铁 Ferrous sulfate | 0.08 ± 0.02 c | 0.06 ± 0.00 c | 0.32 ± 0.01 a | 0.07 ± 0.01 a | 0.12 ± 0.01 b | ||||||||
松土精 Grondverbeteraar | 0.07 ± 0.00 d | 0.07 ± 0.01 b | 0.24 ± 0.01 c | 0.06 ± 0.01 b | 0.05 ± 0.03 c | ||||||||
枯草芽孢杆菌 Bacillus | 0.07 ± 0.00 d | 0.07 ± 0.01 b | 0.22 ± 0.02 d | 0.05 ± 0.01 b | 0.06 ± 0.01 c | ||||||||
农家肥 Farmyard manure | 0.10 ± 0.02 b | 0.09 ± 0.02 ab | 0.18 ± 0.0 1d | 0.05 ± 0.01 b | 0.25 ± 0.02 a |
Fig. 2 Effects of different improvement measures on α diversity of soil bacteria in continuous cropping T1:Bio-organic fertilizer;T2:Ferrous sulfate;T3:Grondverbeteraar;T4:Bacillus;T5:Farmyard manure. The same below.
Fig. 4 Effects of different improvement measures on molecular ecological network of soilbacterial community in continuous cropping Each circle in the figure represents an OTU,and circles with the same color belong to the same phylum. The lines between the circles are Spearman coefficients between OTUs,with the pink line indicating positive correlation and the blue line indicating negative correlation.The thickness of the lines is related to the value of r. More lines indicate that the node is more correlated with other nodes,and the centrality is stronger,so the node is larger.
Fig. 5 Effects of different fertilizer or reagent treatments on the functions of primary metabolic pathway of soil bacteria in peach continuous cropping
Fig. 6 Mental analysis of soil bacterial communities and environmental factors under different improvement measures The line width corresponds to Mental’s r statistic,and the line color corresponds to Mental’s p. Environmental factors were compared pairwise,and the color gradient indicates Pearson’s r correlation coefficient.TN:Total nitrogen;TP:Total phosphorus;TK:Total potassium;AN:Alkaline nitrogen decomposition;AP:Effective phosphorus;AK:Quick-acting potassium;OM:Organic matter;TS:Total salt content;EC:Electrical conductivity. The same below.
Fig. 7 RDA analysis of soil bacterial communities and environmental factors The red arrows represent environmental factors and the blue arrows represent microbiome. Positive correlation for arrow angles less than 90°,negative correlation for arrow angles greater than 90°.
处理 Treatment | 株高/cm Plant height | 茎粗/cm Stem thickness | 叶绿素a含量/(mg · L-1) Chlorophyll a content | 叶绿素b含量/(mg · L-1) Chlorophyll b content |
---|---|---|---|---|
对照Control | 72.76 ± 0.53 d | 0.78 ± 0.02 f | 14.47 ± 0.37 f | 2.64 ± 0.00 f |
生物有机肥 Bio-organic fertilizer | 162.91 ± 5.62 a | 1.58 ± 0.05 b | 23.69 ± 0.31 a | 4.44 ± 0.00 a |
硫酸亚铁 Ferrous sulfate | 88.32 ± 12.62 c | 1.27 ± 0.03 d | 16.56 ± 0.31 e | 3.08 ± 0.18 d |
松土精 Grondverbeteraar | 79.02 ± 3.68 d | 1.18 ± 0.01 e | 19.54 ± 0.28 c | 2.87 ± 0.00 e |
枯草芽孢杆菌 Bacillus | 129.85 ± 7.39 b | 1.37 ± 0.01 c | 19.55 ± 0.01 c | 3.21 ± 0.00 c |
农家肥 Farmyard manure | 179.75 ± 7.02 a | 1.76 ± 0.06 a | 22.77 ± 0.20 b | 4.02 ± 0.15 b |
Table 3 Effect of different fertiliser or reagent treatments on peach seedling growth
处理 Treatment | 株高/cm Plant height | 茎粗/cm Stem thickness | 叶绿素a含量/(mg · L-1) Chlorophyll a content | 叶绿素b含量/(mg · L-1) Chlorophyll b content |
---|---|---|---|---|
对照Control | 72.76 ± 0.53 d | 0.78 ± 0.02 f | 14.47 ± 0.37 f | 2.64 ± 0.00 f |
生物有机肥 Bio-organic fertilizer | 162.91 ± 5.62 a | 1.58 ± 0.05 b | 23.69 ± 0.31 a | 4.44 ± 0.00 a |
硫酸亚铁 Ferrous sulfate | 88.32 ± 12.62 c | 1.27 ± 0.03 d | 16.56 ± 0.31 e | 3.08 ± 0.18 d |
松土精 Grondverbeteraar | 79.02 ± 3.68 d | 1.18 ± 0.01 e | 19.54 ± 0.28 c | 2.87 ± 0.00 e |
枯草芽孢杆菌 Bacillus | 129.85 ± 7.39 b | 1.37 ± 0.01 c | 19.55 ± 0.01 c | 3.21 ± 0.00 c |
农家肥 Farmyard manure | 179.75 ± 7.02 a | 1.76 ± 0.06 a | 22.77 ± 0.20 b | 4.02 ± 0.15 b |
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