Nitrogen Allocation Characteristics and Expression of Related Genes During Corm-Forming Stage of Amorphophallus konjac

doi:10.16420/j.issn.0513-353x.2023-0629

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 2019-2030.doi: 10.16420/j.issn.0513-353x.2023-0629

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Nitrogen Allocation Characteristics and Expression of Related Genes During Corm-Forming Stage of Amorphophallus konjac

REN Siyuan¹, CHEN Sen¹, LONG Zhijian¹, WANG Boya¹, TANG Dengguo¹, WANG Zhengqian², YANG Bin³, HU Shanglian¹, CAO Ying¹^,^*()

¹ School of Life Science and Engineering，Southwest University of Science and Technology，Mianyang，Sichuan 621010，China
² Guangyuan Yinong Biotechnology Co.，Ltd.，Guangyuan，Sichuan 628017，China
³ Xuanhan Agricultural Science and Technology Research Institute，Xuanhan，Sichuan 636150，China

Received:2023-08-29 Revised:2024-07-12 Online:2024-09-25 Published:2024-09-19
Contact: CAO Ying

Abstract

Abstract:

Through ¹⁵N isotopic tracing and RNA-seq analysis，the N distribution in various organs and the expression of genes related to N during the development of konjac corms are systematically studied would help to understand nitrogen utilization and guide the fertilizer use. Results show that during the development of Amorphophallus konjac corms the N content per unit weight of leaves was the highest as the compared with other organs. During the expansion of corms，N content，Ndff value，¹⁵N distribution rate and ¹⁵N utilization rate in leaves showed a downward trend，whereas the N demand in corms increased，the peak of ¹⁵N utilization rate appearing right in the middle stage of corm expansion（45-75 d，day after expansion of leaves）. A. konjac reached the peak of ¹⁵N content of in a whole plant at 60 d，while the ¹⁵N utilization rate also reached the highest rate of 22.68%. Then，both the content and utilization rate of ¹⁵N were deceasing，indicating that this point was the most efficiently nitrogen use period. After 60 d，the absorbed nitrogen was distributed from leaves to corms，which also invalided that 60 d was the suitable period for N topdressing. Meanwhile，the peak of expression in N metabolism-related genes in leaves appeared between the middle and late stage of corm expansion（105 d），a range within which leaf N metabolism was strengthened，promoting the synthesis and output of N assimilation products. Among them，the expression levels in leaves of nitrate reductase gene AkNIA1 and glutamine synthetase gene AkGSR2-1 were significantly and positively correlated with the N content in leaves and roots，indicating their key role in nitrogen absorption and accumulation，and they can be used as candidate genes for genetic improvement of N utilization.

Key words: Amorphophallus konjac, nitrogen distribution, nitrogen metabolism, gene expression, corm, ¹⁵N isotopic tracing

REN Siyuan, CHEN Sen, LONG Zhijian, WANG Boya, TANG Dengguo, WANG Zhengqian, YANG Bin, HU Shanglian, CAO Ying. Nitrogen Allocation Characteristics and Expression of Related Genes During Corm-Forming Stage of Amorphophallus konjac[J]. Acta Horticulturae Sinica, 2024, 51(9): 2019-2030.

Figures/Tables 10

References 32

[1]	Cui Ming, Zhao Xingxi, Du Dajun, Liu Lieping, Wang Xiaobing, Li Zengyi. 2006. Study on the effect of nitrogen,phosphorus and potassium fertilizers on the yield of konjac. Research on Soil and Water Conservation,(6):185-187. (in Chinese)
	崔鸣, 赵兴喜, 都大俊, 刘列平, 王小兵, 李增义. 2006. 氮磷钾肥料对魔芋产量的影响效应研究. 水土保持研究,(6):185-187.
[2]	Dai Xuefeng, Zhu Li, Zhang Shenglin, Niu Yi, Liu Haili. 2021. Screening of antagonistic actinomycetes against soft rot of konjac. Journal of Southwest University(Natural Science Edition), 43 (11):9-17. (in Chinese)
	代雪凤, 朱丽, 张盛林, 牛义, 刘海利. 2021. 魔芋软腐病拮抗放线菌筛选. 西南大学学报(自然科学版), 43 (11):9-17.
[3]	Ding Yonggang. 2022. Characteristics of high-yield and high-efficiency wheat varieties and their nitrogen response mechanism[Ph. D. Dissertation]. Yangzhou: Yangzhou University. (in Chinese)
	丁永刚. 2022. 小麦高产高效品种特征及其氮响应机制[博士论文]. 扬州: 扬州大学.
[4]	Duan X Z, Sun J T, Wang L T, Shu X H, Guo Y, Keiichiro M, Zhu Y X, Bing X L, Hoffmann A A, Hong X Y. 2020. Recent infection by Wolbachia alters microbial communities in wild Laodelphax striatellus populations. Microbiome, 8 (1):104.
[5]	Gao Y, Zhang Y N, Feng C, Chu H L, Feng C, Wang H B, Wu L F, Yin S, Liu C, Chen H H, Li Z M, Zou Z R, Tang L Z. 2022. A chromosome-level genome assembly of Amorphophallus konjac provides insights into konjac glucomannan biosynthesis. Computational and Structural Biotechnology Journal,(20):1002-1011.
[6]	Ghorbani M, Nezhad M P, Mahmoodzadeh F. 2021. Incorporation of oxidized pectin to reinforce collagen/Konjac glucomannan hydrogels designed for tissue engineering applications. Macromolecular Research, 29 (4):289-296.
[7]	Huang Jian, Duan Jingjing, Ma Xiaodong, Qi Tong, Fu Yanbo. 2020. Effects of nitrogen on key enzyme activities of nitrogen assimilation of Salicornia halophila at seedling stage. Xinjiang Agricultural Sciences, 57 (7):1314-1320. (in Chinese)
	黄建, 段婧婧, 马晓东, 祁通, 付彦博. 2020. 盐环境下氮素对苗期盐角草氮同化关键酶活性的影响. 新疆农业科学, 57 (7):1314-1320. doi: 10.6048/j.issn.1001-4330.2020.07.016
[8]	Jiang H B, Li H X, Zhao M X, Mei L X, Kang Y L, Dong C X, Xu Y C. 2020. Strategies for timing nitrogen fertilization of pear trees based on the distribution,storage,and remobilization of ¹⁵N from seasonal application of (¹⁵NH₄)₂SO₄. Journal of Integrative Agriculture, 19 (5):1340-1353.
[9]	Jiang X, Wang Z X, Yang F Q, Chen Z Z, Yang S M, Yan Z S, Qin Y G. 2021. Effects of different pesticide treatments on soft rot control and yield of konjac. IOP Conference Series: Earth and Environmental Science, 687 (1):012049.
[10]	Lee S, Park J, Lee J, Shin D, Marmagne A, Lim P O, Masclanx-Danbresse C, An G, Nam H G. 2020. OsASN1 overexpression in rice increases grain protein content and yield under nitrogen-limiting conditions. Plant and Cell Physiology, 61 (7):1309-1320.
[11]	Li Chengchen, Suo Haicui, Luo Huanming, An Kang, Liu Jitao, Wang Li, Shan Jianwei, Yang Shaohai, Li Xiaobo. 2021. Effects of fertilizer reduction and fertilization methods on potato yield and tuber nitrogen accumulation. China Agricultural Science and Technology Herald, 23 (9):173-183. (in Chinese)
	李成晨, 索海翠, 罗焕明, 安康, 刘计涛, 王丽, 单建伟, 杨少海, 李小波. 2021. 化肥减施和施肥方式对马铃薯产量和块茎氮素积累的影响. 中国农业科技导报, 23 (9):173-183.
[12]	Li Yongjun, Ma Jiqiong, Chen Jianhua, Yin Guifang, Fang Shunquan, Xu Yun, Wang Ling. 2010. Study on the effect of nitrogen application rate on disease occurrence,yield and viscosity of konjac. Southwest Agricultural Journal, 23 (1):128-131. (in Chinese)
	李勇军, 马继琼, 陈建华, 尹桂芳, 方顺权, 徐云, 王玲. 2010. 施氮量对魔芋病害发生、产量及粘度影响的研究. 西南农业学报, 23 (1):128-131.
[13]	Liu Peiying. 2004. Amorphophallus. Beijing: China Agriculture Press. (in Chinese)
	刘佩瑛. 2004. 魔芋学. 北京: 中国农业出版社.
[14]	Liu R X, Li H L, Rui L, Liu G D, Wang T, Wang X F, Li L G, Zhang Z L, You C X. 2023. An apple NITRATE REDUCTASE 2gene positively regulates nitrogen utilization and abiotic stress tolerance in Arabidopsis and apple callus. Plant Physiology and Biochemistry,196:23-32.
[15]	Liu Z Y, Zhu Y A, Dong Y, Tang L, Zheng Y, Xiao J X. 2021. Interspecies interaction for nitrogen use efficiency via up-regulated glutamine and glutamate synthase under wheat-faba bean intercropping. Field Crops Research,274:108324.
[16]	Ma S J, Zheng Y R, Zhou R, Ma M. 2021. Characterization of chitosan films incorporated with different substances of konjac glucomannan,cassava starch,maltodextrin and gelatin,and application in Mongolian cheese packaging. Coatings, 11 (1):84.
[17]	Men Yongge, An Xin, Xu Haigang, Jiang Han, Wei Shaochong, Jiang Yuanmao. 2015. Effects of different loads on the distribution and utilization of ¹³C and ¹⁵N in apples. Acta Plant Nutrition and Fertilizer, 21 (3):702-708. (in Chinese)
	门永阁, 安欣, 许海港, 姜翰, 魏绍冲, 姜远茂. 2015. 不同负载量对苹果¹³C和¹⁵N分配、利用的影响. 植物营养与肥料学报, 21 (3):702-708.
[18]	Tan Yan, Liu Xi, Yuan Fang. 2019. Structure,properties and application of konjac glucomannan in food. China Condiment, 44 (2):168-174,178. (in Chinese)
	谭燕, 刘曦, 袁芳. 2019. 魔芋葡甘聚糖的结构、性质及其在食品中的应用. 中国调味品, 44 (2):168-174,178.
[19]	Tang Danfeng, Zhang Xinming, Chen Hong, Quan Feng, Wu Youguo, Cao Xianwei. 2013. Study on nitrogen absorption,accumulation and distribution characteristics of winter potato. Acta Tropical Crops, 34 (6):1041-1044. (in Chinese)
	汤丹峰, 张新明, 陈洪, 全锋, 伍尤国, 曹先维. 2013. 冬种马铃薯的氮素吸收、积累、分配特征研究. 热带作物学报, 34 (6):1041-1044.
[20]	Wang Qiandeng, Chen Bolang, Yusupujiang Yusuyin, Wang Cheng, Chai Zhongping. 2018. Absorption,distribution and utilization characteristics of ¹⁵N-urea applied to Korla fragrant pear in spring. Journal of Applied Ecology, 29 (5):1443-1449. (in Chinese)
	王前登, 陈波浪, 玉素甫江 · 玉素音, 王成, 柴仲平. 2018. 库尔勒香梨春季施用¹⁵N-尿素的吸收、分配和利用特性. 应用生态学报, 29 (5),1443-1449. doi: 10.13287/j.1001-9332.201805.009
[21]	Wu D X, Li Y, Cao Y N, Hu R P, Wu X, Zhang W, Tao W Q, Xu G H, Wang X C, Zhang Y L. 2021. Increased glutamine synthetase by overexpression of TaGS1 improves grain yield and nitrogen use efficiency in rice. Plant Physiology and Biochemistry,(169):259-268.
[22]	Xu Kaiyue, Wang Xiaohua, Song Biao, Guo Shiwei, Zheng Chaoyuan, Guo Jiuxin, Wu Liangquan, Su Da. 2021. Effects of topdressing nitrogen fertilizer at different stages on nitrogen distribution in mature pomelo trees. Journal of Plant Nutrition and Fertilizer, 27 (4):553-564. (in Chinese)
	徐凯悦, 王晓华, 宋彪, 郭世伟, 郑朝元, 郭九信, 吴良泉, 苏达. 2021. 不同时期追施氮肥对成熟期蜜柚树体氮素分配的影响. 植物营养与肥料学报, 27 (4):553-564.
[23]	Xin Shanshan. 2019. Review of physiological effects of konjac glucomannan. Grain and Food Industry, 26 (5):50-52. (in Chinese)
	信珊珊. 2019. 魔芋葡甘聚糖的生理功效综述. 粮食与食品工业, 26 (5):50-52.
[24]	Yang D, Yuan Y, Wang L, Wang X S, Mu R J, Pang J, Xiao J B, Zheng Y F. 2017. A review on konjac glucomannan gels:microstructure and application. International Journal of Molecular Sciences, 18 (11):2250.
[25]	Yang X L, Nian J Q, Xie Q J, Feng J, Zhang F X, Jiang H W, Zhang J, Dong G J, Liang Y, Peng J, Wang G D, Qian Q, Zuo J R. 2016. Rice ferredoxin-dependent glutamate synthase regulates nitrogen-carbon metabolomes and is genetically differentiated between Japonica and Indica subspecies. Molecular Plant, 9 (11):1520-1534.
[26]	Yang Xiulian, Cun Xiangqin, Liang Yanli, Xie Shiqing, Zhao Qingyun. 2014. Effects of different application rates of nitrogen,phosphorus and potassium fertilizer on soft rot of konjac. Changjiang Vegetables,(22):69-71. (in Chinese)
	杨秀莲, 寸湘琴, 梁艳丽, 谢世清, 赵庆云. 2014. 不同氮、磷、钾肥施用量对魔芋软腐病的影响. 长江蔬菜,(22):69-71.
[27]	Yuan Haotian, Meng Meilian, Chen Youjun, Tan Weilin, Wang Zhanzhong. 2020. Nitrogen absorption and transport of different potato varieties. China Potato, 34 (1):22-30. (in Chinese)
	袁昊田, 蒙美莲, 陈有君, 谭伟林, 王占忠. 2020. 马铃薯不同品种氮素吸收转运规律. 中国马铃薯, 34 (1):22-30.
[28]	Zhao Dawei, Liu Weilin, Qing Fangju, Chen Rui, Xu Jie, Meng Fanlai, Yang Tongshu. 2021. Analysis of high-yield and high-efficiency mode of intercropping waxy corn with flowers and konjac. Tropical Agricultural Science, 41 (1):24-30. (in Chinese)
	赵大伟, 刘炜林, 青方具, 陈瑞, 徐杰, 孟凡来, 杨童舒. 2021. 花魔芋间作糯玉米的高产高效模式分析. 热带农业科学, 41 (1):24-30.
[29]	Zhang C X, Meng S, Li Y M, Su L, Zhao Z. 2016. Nitrogen uptake and allocation in Populus simonii in different seasons supplied with isotopically labeled ammonium or nitrate. Trees,(30):2011-2018.
[30]	Zhang M W, Ma D Y, Ma G, Wang C Y, Xie X D, Kang G Z. 2017. Responses of glutamine synthetase activity and gene expression to nitrogen levels in winter wheat cultivars with different grain protein content. Journal of Cereal Science,(7):187-193.
[31]	Zhang Tingting, Meng Lili, Liu Xiaorui, Chen Youjun, Liu Kunyu, Yuan Haotian, Meng Meilian. 2022. Response of potato nitrogen metabolism to low nitrogen stress and transcriptome analysis. Journal of Northwest A & F University(Natural Science Edition), 50 (8):15-26. (in Chinese)
	张婷婷, 孟丽丽, 刘晓蕊, 陈有君, 刘坤雨, 袁昊田, 蒙美莲. 2022. 马铃薯氮代谢对低氮胁迫的响应及转录组分析. 西北农林科技大学学报(自然科学版), 50 (8):15-26.
[32]	Zhang Zhongxue, Chen Peng, Chen Shuaihong, Zheng Ennan, Nie Tangzhe, Liu Ming. 2018. ¹⁵N tracer analysis of nitrogen absorption and distribution of rice in different periods under water-saving irrigation. Journal of Agricultural Machinery, 49 (6):309-317,346. (in Chinese)
	张忠学, 陈鹏, 陈帅宏, 郑恩楠, 聂堂哲, 刘明. 2018. ¹⁵N示踪分析节水灌溉下水稻对不同时期氮肥的吸收分配. 农业机械学报, 49 (6),309-317,346.

引物名称 Primer name	引物序列（5′-3′） Primer sequence	引物名称 Primer name	引物序列（5′-3′） Primer sequence
AkNIA1-F	GTGTGGTACGTGGTGAATGAG	AkGSR2-1-F	TGGAACTATGATGGATCGAGCA
AkNIA1-R	GTTCTTAACGTCGTAGCCCATC	AkGSR2-1-R	TGTGTACTCCTGTTCAATCCCA
AkNIR1-F	GAAGGTGAAGCTGGAGAAGGAG	AkASN1-F	AGAGAATTTCGTGGACATGCTG
AkNIR1-R	CTTCAGCCTCATCATGAACCG	AkASN1-R	AAATGCTCGCAGTCATCATTGA
AkGLU1-F	GTTGTCCTTGGAAAAGTGGGAA	Tubulin-F	GCCGTGAATCTCATCCCCTT
AkGLU1-R	ATCCTGCCCATCACAAATTCTG	Tubulin-R	TTGTTCTTGGCATCCCACAT

引物名称 Primer name	引物序列（5′-3′） Primer sequence	引物名称 Primer name	引物序列（5′-3′） Primer sequence
AkNIA1-F	GTGTGGTACGTGGTGAATGAG	AkGSR2-1-F	TGGAACTATGATGGATCGAGCA
AkNIA1-R	GTTCTTAACGTCGTAGCCCATC	AkGSR2-1-R	TGTGTACTCCTGTTCAATCCCA
AkNIR1-F	GAAGGTGAAGCTGGAGAAGGAG	AkASN1-F	AGAGAATTTCGTGGACATGCTG
AkNIR1-R	CTTCAGCCTCATCATGAACCG	AkASN1-R	AAATGCTCGCAGTCATCATTGA
AkGLU1-F	GTTGTCCTTGGAAAAGTGGGAA	Tubulin-F	GCCGTGAATCTCATCCCCTT
AkGLU1-R	ATCCTGCCCATCACAAATTCTG	Tubulin-R	TTGTTCTTGGCATCCCACAT

散叶后天数/d Days after expansion of leaves	叶片 Leaf	叶柄 Petiole	根 Root	球茎 Corm
30	35.33 ± 0.83 a	15.58 ± 1.09 c	29.00 ± 3.95 a	12.21 ± 0.96 c
45	34.90 ± 0.58 a	19.02 ± 1.59 b	28.84 ± 0.79 a	21.06 ± 5.56 a
60	35.68 ± 2.11 a	21.79 ± 1.04 a	29.36 ± 4.71 a	17.25 ± 0.14 abc
75	29.19 ± 0.71 b	14.55 ± 1.15 c	26.47 ± 2.44 a	15.09 ± 2.19 bc
105	23.84 ± 1.30 c	15.69 ± 0.94 c	23.81 ± 2.78 a	17.95 ± 1.51 ab

散叶后天数/d Days after expansion of leaves	叶片 Leaf	叶柄 Petiole	根 Root	球茎 Corm
30	35.33 ± 0.83 a	15.58 ± 1.09 c	29.00 ± 3.95 a	12.21 ± 0.96 c
45	34.90 ± 0.58 a	19.02 ± 1.59 b	28.84 ± 0.79 a	21.06 ± 5.56 a
60	35.68 ± 2.11 a	21.79 ± 1.04 a	29.36 ± 4.71 a	17.25 ± 0.14 abc
75	29.19 ± 0.71 b	14.55 ± 1.15 c	26.47 ± 2.44 a	15.09 ± 2.19 bc
105	23.84 ± 1.30 c	15.69 ± 0.94 c	23.81 ± 2.78 a	17.95 ± 1.51 ab

散叶后天数/d Days after expansion of leaves	叶片 Leaf	叶柄 Petiole	根 Root	球茎 Corm	全株 Whole plant
30	1.17 ± 0.04 a	1.12 ± 0.10 ab	1.12 ± 0.06 ab	0.89 ± 0.11 b	4.31 ± 0.19 a
45	0.87 ± 0.22 b	1.34 ± 0.17 a	1.06 ± 0.16 b	1.22 ± 0.04 a	4.49 ± 0.58 a
60	0.85 ± 0.12 b	1.36 ± 0.16 a	1.27 ± 0.09 a	1.34 ± 0.08 a	4.81 ± 0.44 a
75	0.59 ± 0.11 c	0.87 ± 0.17 b	0.55 ± 0.02 d	0.86 ± 0.12 b	2.88 ± 0.38 b
105	0.56 ± 0.04 c	0.90 ± 0.05 b	0.78 ± 0.06 c	0.60 ± 0.02 c	2.84 ± 0.07 b

Nitrogen Allocation Characteristics and Expression of Related Genes During Corm-Forming Stage of Amorphophallus konjac

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散叶后天数/d Days after expansion of leaves	叶片 Leaf	叶柄 Petiole	根 Root	球茎 Corm
30	67.09 ± 0.38 a	8.30 ± 0.24 b	11.55 ± 1.11 bc	13.06 ± 0.83 e
45	45.98 ± 3.00 b	11.99 ± 1.28 a	12.68 ± 0.04 b	29.34 ± 0.46 d
60	26.62 ± 1.42 c	13.03 ± 2.12 a	14.97 ± 0.78 a	45.38 ± 3.32 c
75	24.04 ± 0.36 c	8.53 ± 0.63 b	10.77 ± 0.95 c	56.66 ± 5.09 b
105	19.12 ± 0.52 d	5.51 ± 0.13 c	12.13 ± 0.62 bc	63.24 ± 4.62 a

散叶后天数/d Days after expansion of leaves	叶片 Leaf	叶柄 Petiole	根 Root	球茎 Corm	全株 Whole plant
30	10.57 ± 0.06 a	1.31 ± 0.04 bc	1.82 ± 0.18 c	2.06 ± 0.13 d	15.75 ± 0.03 cd
45	6.37 ± 0.42 b	1.66 ± 0.18 b	1.76 ± 0.01 c	4.06 ± 0.07 c	13.85 ± 0.66 d
60	6.04 ± 0.33 b	2.96 ± 0.48 a	3.40 ± 0.18 a	10.29 ± 0.75 b	22.68 ± 1.73 a
75	4.92 ± 0.07 c	1.75 ± 0.13 b	2.21 ± 0.20 b	11.60 ± 1.04 a	20.47 ± 1.44 b
105	3.11 ± 0.08 d	0.90 ± 0.02 c	1.97 ± 0.10 bc	10.29 ± 0.75 b	16.27 ± 0.76 c

基因家族 Gene family	基因名称 Gene name	基因编号 Gene identifier	等电点 pI	分子量/ kD MW	氨基酸数 Amino acid number	亚细胞定位 Subcellular localization
NR	AkNIA1	evm.model.HIC_ASM_3.7576_Akon	10.05	56.88	507	细胞质Cytoplasm
	AkNIA2	evm.model.HIC_ASM_3.7577_Akon	8.85	53.58	486	细胞质Cytoplasm
	AkNIA3	evm.model.CTG_10382.10_Akon	8.91	14.69	131	细胞膜Cell membrane
	AkNIA4	evm.model.CTG_15369.3_Akon	7.87	13.07	118	细胞膜Cell membrane
NIR	AkNIR1	evm.model.HIC_ASM_7.230_Akon	6.60	67.20	609	叶绿体，线粒体Chloroplast，mitochondrion
NIR	AkNIR2	evm.model.CTG_4710.3_Akon	6.60	67.20	609	叶绿体，线粒体Chloroplast，mitochondrion
GS	AkGSR2-1	evm.model.HIC_ASM_2.6744_Akon	6.68	47.74	438	叶绿体，线粒体Chloroplast，mitochondrion
	AkGSR2-2	evm.model.HIC_ASM_2.6760_Akon	6.68	47.81	438	叶绿体，线粒体Chloroplast，mitochondrion
	AkGSR2-3	evm.model.HIC_ASM_3.9051_Akon	6.22	39.42	358	叶绿体，细胞质Chloroplast，cytoplasm
	AkGSR2-4	evm.model.HIC_ASM_5.508_Akon	5.22	24.29	219	叶绿体，线粒体Chloroplast，mitochondrion
	AkGSR2-5	evm.model.HIC_ASM_5.6034_Akon	5.53	39.41	358	叶绿体Chloroplast
	AkGSR2-6	evm.model.HIC_ASM_7.1708_Akon	10.28	10.35	96	叶绿体Chloroplast
	AkGSR2-7	evm.model.HIC_ASM_7.4324_Akon	5.88	17.67	163	叶绿体Chloroplast
	AkGSR2-8	evm.model.HIC_ASM_7.7618_Akon	5.48	39.15	357	细胞质Cytoplasm
	AkGSR2-9	evm.model.CTG_9235.4_Akon	8.65	26.06	239	叶绿体Chloroplast
	AkGSR2-10	evm.model.CTG_10208.4_evm.model.CTG_10208.5_Akon	5.36	39.23	357	细胞质Cytoplasm
	AkGSR2-11	evm.model.CTG_18461.3.3_Akon	5.65	93.14	841	叶绿体Chloroplast
GOGAT	AkGLU1	evm.model.HIC_ASM_2.6868_Akon	6.70	176.22	1 626	叶绿体Chloroplast
GOGAT	AkGLT1	evm.model.HIC_ASM_8.1189_Akon	6.47	242.63	2 209	叶绿体Chloroplast
AS	AkASN1	evm.model.HIC_ASM_4.2066_Akon	6.09	70.65	634	叶绿体Chloroplast
AS	AkASN2	evm.model.HIC_ASM_4.950_Akon	5.83	66.02	592	叶绿体，细胞质Chloroplast，cytoplasm

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