Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 1983-1996.doi: 10.16420/j.issn.0513-353x.2023-0085
• Genetic & Breeding · Germplasm Resources · Molecular Biology • Next Articles
LIU Jianhao1,2, JING Yanfu2, LIU Yuexin1,2, XU Yaoguang2, YU Yang2, GE Xiuxiu1,**(), XIE Hua1,2,**(
)
Received:
2024-05-17
Revised:
2024-07-16
Online:
2024-09-25
Published:
2024-09-19
Contact:
GE Xiuxiu, XIE Hua
LIU Jianhao, JING Yanfu, LIU Yuexin, XU Yaoguang, YU Yang, GE Xiuxiu, XIE Hua. Identification of Peach NAC Gene Family and Role of PpNAC050 in Promoting Fruit Fructose Accumulation[J]. Acta Horticulturae Sinica, 2024, 51(9): 1983-1996.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0085
基因 Gene | 引物序列(5′-3′) Primer sequence | PCR产物大小/bp PCR product size |
---|---|---|
PpNAC-BD-F | ATCTCAGAGGAGGACCTGCATATGATGAGTATGGCCAATGCCATG | 1 638 |
PpNAC-BD-R | CGGCCGCTGCAGGTCGACGGATCCAGAACTTTGTGGGAAAAGAGTCGC | |
PpNAC-F | ATGAGTATGGCCAATGCCATG | 1 641 |
PpNAC-R | TCAAGAACTTTGTGGGAAAAGAGTCGC | |
PpNAC-1300-F | TAAATACTAGTGGATCCGGTACCATGAGTATGGCCAATGCCATG | 1 638 |
PpNAC-1300-R | TCGCCCTTGCTCACCATGGTACCAGAACTTTGTGGGAAAAGAGTCGC | |
PpNAC-62SK-F | GGCCGCTCTAGAACTAGTGGATCCATGAGTATGG CCAATGCCAT GT | 1 638 |
PpNAC-62SK-R | ACCGGGCCCCCCCTCGAGGTCGACAGAACTTT GTGGGAAAAG AGTC | |
q-NAC-F | TGAGTATGGCCAATGCCATGT | 181 |
q-NAC-R | TTGGGCAGCAAATCAAGCAC | |
PpNAC-AD-F | CCATGGAGGCCAGTGAATTCATGAGTATGGCCAATGCCATGTAT | 1 638 |
PpNAC-AD-R | TGCCCACCCGGGTGGAATTC AGAACTTTGTGGGAAAAGAGTCG | |
PpERDL6-1-NAC-F | GACATGTGTATAAATACCTCAGAACACGAGCATATATTGCATTGCATCCC | 50 |
PpERDL6-1-NAC-R | GGGATGCAATGCAATATATGCTCGTGTTCTGAGGTATTTATACACATGTC | |
4T-PpNAC-F | CCGCGTGGATCCCCGGAATTCATGAGTATGGCCAATGCCATG | 1 638 |
4T-PpNAC-R | GTCACGATGCGGCCGCTCGAGAGAACTTTGTGGGAAAAGAGTCGC | |
PpERDL6-1-800-lucF | GGTCGACGGTATCGATAAGCTT GTCACGTCTG ATGTCCCAGT | 1 971 |
PpERDL6-1-800-lucR | GCAGGAATTCGATATCAAGCTT GTTTTTTTCTC ACTACTCTAA | |
PpERDL6-1-q-F | TCACTATGGTCGGAGCAACG | 183 |
PpERDL6-1-q-R | ATGTAAGTCAGCACGCCAGA |
Table 1 Primer sequences
基因 Gene | 引物序列(5′-3′) Primer sequence | PCR产物大小/bp PCR product size |
---|---|---|
PpNAC-BD-F | ATCTCAGAGGAGGACCTGCATATGATGAGTATGGCCAATGCCATG | 1 638 |
PpNAC-BD-R | CGGCCGCTGCAGGTCGACGGATCCAGAACTTTGTGGGAAAAGAGTCGC | |
PpNAC-F | ATGAGTATGGCCAATGCCATG | 1 641 |
PpNAC-R | TCAAGAACTTTGTGGGAAAAGAGTCGC | |
PpNAC-1300-F | TAAATACTAGTGGATCCGGTACCATGAGTATGGCCAATGCCATG | 1 638 |
PpNAC-1300-R | TCGCCCTTGCTCACCATGGTACCAGAACTTTGTGGGAAAAGAGTCGC | |
PpNAC-62SK-F | GGCCGCTCTAGAACTAGTGGATCCATGAGTATGG CCAATGCCAT GT | 1 638 |
PpNAC-62SK-R | ACCGGGCCCCCCCTCGAGGTCGACAGAACTTT GTGGGAAAAG AGTC | |
q-NAC-F | TGAGTATGGCCAATGCCATGT | 181 |
q-NAC-R | TTGGGCAGCAAATCAAGCAC | |
PpNAC-AD-F | CCATGGAGGCCAGTGAATTCATGAGTATGGCCAATGCCATGTAT | 1 638 |
PpNAC-AD-R | TGCCCACCCGGGTGGAATTC AGAACTTTGTGGGAAAAGAGTCG | |
PpERDL6-1-NAC-F | GACATGTGTATAAATACCTCAGAACACGAGCATATATTGCATTGCATCCC | 50 |
PpERDL6-1-NAC-R | GGGATGCAATGCAATATATGCTCGTGTTCTGAGGTATTTATACACATGTC | |
4T-PpNAC-F | CCGCGTGGATCCCCGGAATTCATGAGTATGGCCAATGCCATG | 1 638 |
4T-PpNAC-R | GTCACGATGCGGCCGCTCGAGAGAACTTTGTGGGAAAAGAGTCGC | |
PpERDL6-1-800-lucF | GGTCGACGGTATCGATAAGCTT GTCACGTCTG ATGTCCCAGT | 1 971 |
PpERDL6-1-800-lucR | GCAGGAATTCGATATCAAGCTT GTTTTTTTCTC ACTACTCTAA | |
PpERDL6-1-q-F | TCACTATGGTCGGAGCAACG | 183 |
PpERDL6-1-q-R | ATGTAAGTCAGCACGCCAGA |
Fig. 2 Phylogenetic analysis of NAC family members in peach(Prupe)and Arabidopsis(AT) a:ANAC001/ONAC003;b:TIP;c:OSNAC8;d:NAC2;e:ANAC011;f:NAM/NAC1;g:OSNAC7;h:ONAC022/TERN;j:ANAC063/SENU5;k:ANAC104;l:ATAF/AtNAC3;m:NAP;n:ANAC063.
Fig. 3 Expression(A),fragment amplification(B)and transcriptional activation detection(C)of PpNAC050 S1:First rapid growth phase;S2:Lag phase;S3:Second rapid growth phase. Different lowercase letters indicate in Fig. A significant differences at the 0.05 level in different fruit development periods.
Fig. 4 The subcellular localization of PpNAC050 A:Schematic diagram the recombinant plasmid pCAMBIA1300-PpNAC050-GFP; B:The GFP fluorescence from PpNAC050-GFP fusion protein was localized to the nucleus of the cells.
Fig. 5 The expression level and fructose,glucose and sucrosecontent in peach fruits transiently overexpressing PpNAC050 CK-1,CK-2和CK-3:Empty pGreen0029-62SK;TOE-1,TOE-2 and TOE-3:Overexpression. ns:Non-significant. * P < 0.05,** P < 0.01(Student’s t-test).
Fig. 6 PpNAC050 suppresses the expression of sugar transporter PpERDL6-1 A:The expression levels of sugar transporter PpERDL6-1 in transiently overexpressing PpNAC050 fruits;B and C:Yeast one-hybrid and EMSA assay showing PpNAC050 binds to the PpERDL6-1 promoter fragment,respectively. D:The effects of PpNAC050 on the PpERDL6-1 sugar transporter promoter with dual-luciferase reporter assay in tobacco. Error bars represent the standard deviation of three biological replicates. ** P < 0.01,Student’s t-test.
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