Biocontrol Efficacy and Mechanisms of Streptomyces purpeofuscus CC2-6 Against Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae

doi:10.16420/j.issn.0513-353x.2023-0146

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 1137-1150.doi: 10.16420/j.issn.0513-353x.2023-0146

• Plant Protection • Previous Articles Next Articles

Biocontrol Efficacy and Mechanisms of Streptomyces purpeofuscus CC2-6 Against Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae

WANG Qing¹^,³, HU Yan², CHEN Shan¹, LU Jingwei², ZHENG Yang², TAO Weilin², SUN Xianchao¹, ZHOU Na²^,^**(), CHEN Guokang¹^,^**()

¹ College of Plant Protection，Southwest University，Chongqing 400716，China
² Institute of Vegetable and Flower Research，Chongqing Academy of Agricultural Sciences，Chongqing 400055，China
³ Forestry Technology Extension Station of Jiashan County，Jiashan，Zhejiang 314199，China

Received:2023-10-08 Revised:2024-02-10 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

In order to screen out biocontrol strains that can effectively inhibit Plasmodiophora brassicae，Fusarium oxysporum was used as the indicator fungus，and the strain CC2-6 was screened by plate confrontation method. The inhibitory rate was 50.03%. The fermentation broth of strain CC2-6 could inhibit the germination of resting spores of P. brassicae and the infection of P. brassicae from root hairs，and and the control efficacy of Chinese cabbage clubroot was 53.20% in pot trials. In addition，CC2-6 had inhibitory effects on nine pathogens including Pestalotiopsis nicotiae，and its inhibition rate rangeed more than 47.20%-76.19%，indicating that CC2-6 had broad-spectrum bacteriostatic activity. The strain CC2-6 was preliminarily identified as Streptomyces purpeofuscus by morphological observation and 16S rDNA gene sequence analysis. The strain CC2-6 could produce protease，cellulase and chitinase，and its fermentation broth could induce the increase of PPO，POD and PAL activities，the increase of SA and JA contents and the decrease of ET content in Chinese cabbage. The results of GC-TOF/LC-QTOF/QE non-target metabolomics showed that the fermentation broth of strain CC2-6 contained antagnostic active substances such as hygromycin B，camptothecin，2-phenylethanol and citric acid，indicating that CC2-6 might inhibit the infection of P. brassicae by metabolizing these compounds.

Key words: Plasmodiophora brassicae, biocontrol actinomycetes, Streptomyces purpeofuscus, induced resistance, metabolites

WANG Qing, HU Yan, CHEN Shan, LU Jingwei, ZHENG Yang, TAO Weilin, SUN Xianchao, ZHOU Na, CHEN Guokang. Biocontrol Efficacy and Mechanisms of Streptomyces purpeofuscus CC2-6 Against Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae[J]. Acta Horticulturae Sinica, 2024, 51(5): 1137-1150.

Figures/Tables 10

References 34

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病原菌 Target pathogen	抑制率 Inhibition rate	病原菌 Target pathogen	抑制率 Inhibition rate
棉花枯萎病菌Fusarium oxysporum	50.30 ± 1.50	油菜菌核病菌Sclerotinia sclerotiorum	76.19 ± 1.19
甘薯软腐病菌Rhizopus stolonifer	51.67 ± 0.78	棉花立枯病菌Thanatepehorus cucumeris	52.79 ± 1.51
茄褐纹病菌Phomopsis vexans	55.58 ± 1.24	辣椒疫霉病菌Phytophthora capsici	66.67 ± 1.67
烟草灰霉病菌Botrytis cinerea	53.95 ± 0.63	黄瓜猝倒病菌Pythium delicense	72.31 ± 0.96
烟草炭疽病菌Colletotrichum micotianae	47.20 ± 5.21	烟草叶斑病菌Pestalotiopsis nicotiae	59.71 ± 0.75

病原菌 Target pathogen	抑制率 Inhibition rate	病原菌 Target pathogen	抑制率 Inhibition rate
棉花枯萎病菌Fusarium oxysporum	50.30 ± 1.50	油菜菌核病菌Sclerotinia sclerotiorum	76.19 ± 1.19
甘薯软腐病菌Rhizopus stolonifer	51.67 ± 0.78	棉花立枯病菌Thanatepehorus cucumeris	52.79 ± 1.51
茄褐纹病菌Phomopsis vexans	55.58 ± 1.24	辣椒疫霉病菌Phytophthora capsici	66.67 ± 1.67
烟草灰霉病菌Botrytis cinerea	53.95 ± 0.63	黄瓜猝倒病菌Pythium delicense	72.31 ± 0.96
烟草炭疽病菌Colletotrichum micotianae	47.20 ± 5.21	烟草叶斑病菌Pestalotiopsis nicotiae	59.71 ± 0.75

处理 Treatment	防治效果/% Indoor control effect
同时接菌 Strain CC2-6 was simultaneously inoculated with P. brassicae	27.46 ± 2.37 b
提前7 d接种菌株CC2-6 Strain CC2-6 was inoculated 7 d in advance	53.20 ± 1.82 a

处理 Treatment	防治效果/% Indoor control effect
同时接菌 Strain CC2-6 was simultaneously inoculated with P. brassicae	27.46 ± 2.37 b
提前7 d接种菌株CC2-6 Strain CC2-6 was inoculated 7 d in advance	53.20 ± 1.82 a

编号	代谢物	归一化后的代谢物丰度 Metabolic abundance after normalization
Code	Metabolite components	CC2-6-1	CC2-6-2	CC2-6-3
pos_628	诺氟沙星 Norfloxacin	0.001539502	0.000906463	0.001681919
pos_695	四环素 Tetracycline	2.28324E-05	6.55759E-06	2.04925E-05
pos_690	潮霉素B Hygromycin B	3.22625E-05	1.30073E-07	3.03196E-05
pos_664	托普霉素 Tobramycin	0.000110993	6.0012E-05	6.48009E-05
pos_683	盐霉素 Salinomycin	0.023219173	0.022620707	0.022703959
pos_474	2-苯乙醇 2-Phenylethanol	6.18249E-06	5.00147E-06	4.44714E-06
pos_445	莽草酸 Shikimic acid	3.38127E-06	3.21205E-06	4.07632E-06
pos_708	紫杉醇 Paclitaxel	2.47289E-05	3.74363E-05	3.1205E-05
pos_482	熊果苷 Arbutin	0.000933082	0.00089494	0.00096134
pos_236	L-岩藻糖 L-Fucose	5.18191E-06	3.25384E-06	3.57858E-06
pos_26	麦芽四糖Maltotetraose	0.006656552	0.006144853	0.007064496
pos_345	乙酰丁香酮 Acetosyringone	0.000384261	0.001579729	0.000450847
pos_54	姜油酮 Zingerone	2.82372E-05	2.29699E-05	3.25111E-05
pos_250	香豆酸 Coutaric acid	0.000821003	0.001010641	0.000847733
pos_469	阿斯特酚苷 Astringin	5.47227E-05	5.21228E-06	4.92987E-05

Biocontrol Efficacy and Mechanisms of Streptomyces purpeofuscus CC2-6 Against Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae

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编号	代谢物	归一化后的代谢物丰度 Metabolic abundance after normalization
Code	metabolite components	CC2-6-1	CC2-6-2	CC2-6-3
neg_575	土霉素 Oxytetracycline	1.87E-05	6.75E-07	0.075964
neg_51	放线酰胺素 actinonin	0.023284	0.014191	0.000149
neg_611	氟康唑Fluconazole	0.00664	0.014538	0
neg_511	印楝素A Azadirachtin A	0.00039	0.000211	0.015373
neg_569	柚皮苷 Naringin	0.004988	0.005777	1.48E-05
neg_372	硫乳霉素 Thiolactomycin	2.64E-05	1.16E-05	0.003713
neg_518	银杏内酯B Ginkgolide B	0.001923	0.001084	0.000244
neg_233	4-羟基苯甲酸 4-Hydroxybenzoic acid	0.000101	0.000116	0.001317
neg_510	喜树碱 Camptothecin	0.000331	0.001183	1.92E-05
neg_552	潮霉素B Hygromycin B	0	0	0.001455
neg_159	苯乙胺 Phenylethylamine	0.000451	0.000677	0.000183
neg_9	柠檬酸 Citric acid	0.00079	0.000511	0
neg_152	二羟丙酮 Dihydroxyacetone	0.00047	0.000458	0.000222
neg_446	噻苯咪唑 Thiabendazole	0.00034	0.000636	0.000151
neg_648	淫羊藿苷 Icariin	0.000323	0.000301	0
neg_354	对羟基肉桂酸 4-Hydroxycinnamic acid	1.61E-05	1.5E-05	0.000363
neg_320	新生霉素 Novobiocin	0	0	0.000288
neg_617	多球壳菌素 Myriocin	0	0	0.00028
neg_513	四环素 Tetracycline	0.000129	0.000133	1.25E-06
neg_667	依诺沙星 Enoxacin	7.17E-06	5.7E-06	0.000149

编号	代谢物	归一化后的代谢物丰度 Metabolic abundance after normalization
Code	metabolite components	CC2-6-1	CC2-6-2	CC2-6-3
neg_575	土霉素 Oxytetracycline	1.87E-05	6.75E-07	0.075964
neg_51	放线酰胺素 actinonin	0.023284	0.014191	0.000149
neg_611	氟康唑Fluconazole	0.00664	0.014538	0
neg_511	印楝素A Azadirachtin A	0.00039	0.000211	0.015373
neg_569	柚皮苷 Naringin	0.004988	0.005777	1.48E-05
neg_372	硫乳霉素 Thiolactomycin	2.64E-05	1.16E-05	0.003713
neg_518	银杏内酯B Ginkgolide B	0.001923	0.001084	0.000244
neg_233	4-羟基苯甲酸 4-Hydroxybenzoic acid	0.000101	0.000116	0.001317
neg_510	喜树碱 Camptothecin	0.000331	0.001183	1.92E-05
neg_552	潮霉素B Hygromycin B	0	0	0.001455
neg_159	苯乙胺 Phenylethylamine	0.000451	0.000677	0.000183
neg_9	柠檬酸 Citric acid	0.00079	0.000511	0
neg_152	二羟丙酮 Dihydroxyacetone	0.00047	0.000458	0.000222
neg_446	噻苯咪唑 Thiabendazole	0.00034	0.000636	0.000151
neg_648	淫羊藿苷 Icariin	0.000323	0.000301	0
neg_354	对羟基肉桂酸 4-Hydroxycinnamic acid	1.61E-05	1.5E-05	0.000363
neg_320	新生霉素 Novobiocin	0	0	0.000288
neg_617	多球壳菌素 Myriocin	0	0	0.00028
neg_513	四环素 Tetracycline	0.000129	0.000133	1.25E-06
neg_667	依诺沙星 Enoxacin	7.17E-06	5.7E-06	0.000149