Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2337-2349.doi: 10.16420/j.issn.0513-353x.2022-0981
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
LIU Chenglang1,*, FENG Di1,*, CAO Zonghong1, YAN Suyun3, ZHANG Yongqing1, XU Xiangzeng2, GAO Shide2, YE Junli1, CHAI Lijun1, XIE Zongzhou1,**(), DENG Xiuxin1,**(
)
Received:
2023-05-31
Revised:
2023-08-28
Online:
2023-11-25
Published:
2023-11-28
Contact:
XIE Zongzhou, DENG Xiuxin
LIU Chenglang, FENG Di, CAO Zonghong, YAN Suyun, ZHANG Yongqing, XU Xiangzeng, GAO Shide, YE Junli, CHAI Lijun, XIE Zongzhou, DENG Xiuxin. Comparative Analysis of Genetic Background of‘Dongshi Zaoyou’,‘Shatianyou’and‘Shuijingyou’Pummelos[J]. Acta Horticulturae Sinica, 2023, 50(11): 2337-2349.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0981
引物名称 Primer name | 引物序列(5′-3′) Sequence | 退火温度/℃ Annealing temperature |
---|---|---|
CH1 | F:ATGAATGCTTGTGCAAGGACAGCTAAATTC;R:TTAAAGATTTTCCATCTGGGTAAAAGCAT | 56 |
CH2 | F:GAGGGCAGCAGTTTGAATCC;R:TGGGACCAACTTACATCAATGC | 55 |
CH3 | F:CGTTCGAGTCGTCTTTGTTGG;R:ATACACTGTAAGAGAGATCGTAACA | 56 |
CH4 | F:ATGAATGCTTGTGCAAGGACAGCTAAATTCCAG;R:TTAAAGATTTTCCATCTGGGTAAAAGCATCTTC | 55 |
CH5 | F:CGCGGAGTCAGCAACAAAAA;R:ACACTGTAAGAGAGATCGTAACAT | 55 |
CH6 | F:AGCAACCCGAAACACAAACAC;R:ACACTGTAAGAGAGATCGTAACATT | 55 |
CH7 | F:GGATTTGGCTTTTCATCTTCTCA;R:TGCTAATGAGAGATAGTGGGGAG | 56 |
Table1 InDel primers used in the genetic identification of‘Dongshi Zaoyou’pummelo
引物名称 Primer name | 引物序列(5′-3′) Sequence | 退火温度/℃ Annealing temperature |
---|---|---|
CH1 | F:ATGAATGCTTGTGCAAGGACAGCTAAATTC;R:TTAAAGATTTTCCATCTGGGTAAAAGCAT | 56 |
CH2 | F:GAGGGCAGCAGTTTGAATCC;R:TGGGACCAACTTACATCAATGC | 55 |
CH3 | F:CGTTCGAGTCGTCTTTGTTGG;R:ATACACTGTAAGAGAGATCGTAACA | 56 |
CH4 | F:ATGAATGCTTGTGCAAGGACAGCTAAATTCCAG;R:TTAAAGATTTTCCATCTGGGTAAAAGCATCTTC | 55 |
CH5 | F:CGCGGAGTCAGCAACAAAAA;R:ACACTGTAAGAGAGATCGTAACAT | 55 |
CH6 | F:AGCAACCCGAAACACAAACAC;R:ACACTGTAAGAGAGATCGTAACATT | 55 |
CH7 | F:GGATTTGGCTTTTCATCTTCTCA;R:TGCTAATGAGAGATAGTGGGGAG | 56 |
引物名称 Primer name | 引物序列(5′-3′) Sequence | 退火温度/℃ Annealing temperature |
---|---|---|
S1 | F:ATGAACATTACTTTCTTCCTCTA;R:TTAAGGCGGGGGAAAGGTA | 55 |
S2 | F:ATGATATCGACAAAGACGAAAA;R:CTACTCATCGGTCGGCTCG | 55 |
S5 | F:ATGAAGGTGGCATCCATCAAC;R:CTACCACCGTGGTGGGAAAATAATATCC | 56 |
S6 | F:ATGGGGACTAATTTCCTCATTATC;R:CTATATTTTAACGTATCGCGGC | 55 |
Table 2 The specific primers for S-genotypes identification
引物名称 Primer name | 引物序列(5′-3′) Sequence | 退火温度/℃ Annealing temperature |
---|---|---|
S1 | F:ATGAACATTACTTTCTTCCTCTA;R:TTAAGGCGGGGGAAAGGTA | 55 |
S2 | F:ATGATATCGACAAAGACGAAAA;R:CTACTCATCGGTCGGCTCG | 55 |
S5 | F:ATGAAGGTGGCATCCATCAAC;R:CTACCACCGTGGTGGGAAAATAATATCC | 56 |
S6 | F:ATGGGGACTAATTTCCTCATTATC;R:CTATATTTTAACGTATCGCGGC | 55 |
试验材料 Plant material | 单果质量/g Fruit weight | 纵径/mm Vertical diameter | 横径/mm Transverse diameter | 果形指数 Fruit shape index |
---|---|---|---|---|
‘东试早柚’Dongshi Zaoyou | 1 062.17 ± 25.34 bc | 151.18 ± 9.01 b | 143.66 ± 2.55 b | 1.05 ± 0.06 ab |
‘沙田柚’Shatianyou | 1 543.60 ± 165.96 a | 192.06 ± 8.96 a | 160.61 ± 10.74 a | 1.20 ± 0.05 a |
‘水晶柚1’Shuijingyou 1 | 1 207.26 ± 63.46 bc | 152.99 ± 1.35 b | 144.49 ± 6.25 b | 1.06 ± 0.05 ab |
‘水晶柚2’Shuijingyou 2 | 1 048.00 ± 123.57 c | 155.99 ± 14.24 b | 149.95 ± 4.19 ab | 1.04 ± 0.09 b |
‘水晶柚3’Shuijingyou 3 | 1 349.62 ± 204.80 ab | 187.94 ± 10.11 a | 158.14 ± 7.81 ab | 1.19 ± 0.06 a |
试验材料 Plant material | 可溶性固形物/% Total soluble solid | 可滴定酸/% Titratable acid | 固酸比 Solidity-acid ratio | 维生素C/(mg · kg-1) Vitamin C |
‘东试早柚’Dongshi Zaoyou | 10.93 ± 0.19 a | 0.16 ± 0.02 b | 71.36 ± 9.49 a | 788.8 ± 1.32 a |
‘沙田柚’Shatianyou | 8.55 ± 0.70 b | 0.41 ± 0.18 a | 27.20 ± 15.82 b | 646.0 ± 8.47 b |
‘水晶柚1’Shuijingyou 1 | 9.44 ± 0.40 b | 0.32 ± 0.07 ab | 30.32 ± 5.44 b | 570.7 ± 4.57 b |
‘水晶柚2’Shuijingyou 2 | 8.95 ± 0.24 b | 0.18 ± 0.03 ab | 52.51 ± 10.48 b | 568.2 ± 2.71 b |
‘水晶柚3’Shuijingyou 3 | 8.88 ± 0.62 b | 0.25 ± 0.05 ab | 37.00 ± 5.35 b | 539.6 ± 6.22 b |
Table 3 Fruit quality of different pummelos
试验材料 Plant material | 单果质量/g Fruit weight | 纵径/mm Vertical diameter | 横径/mm Transverse diameter | 果形指数 Fruit shape index |
---|---|---|---|---|
‘东试早柚’Dongshi Zaoyou | 1 062.17 ± 25.34 bc | 151.18 ± 9.01 b | 143.66 ± 2.55 b | 1.05 ± 0.06 ab |
‘沙田柚’Shatianyou | 1 543.60 ± 165.96 a | 192.06 ± 8.96 a | 160.61 ± 10.74 a | 1.20 ± 0.05 a |
‘水晶柚1’Shuijingyou 1 | 1 207.26 ± 63.46 bc | 152.99 ± 1.35 b | 144.49 ± 6.25 b | 1.06 ± 0.05 ab |
‘水晶柚2’Shuijingyou 2 | 1 048.00 ± 123.57 c | 155.99 ± 14.24 b | 149.95 ± 4.19 ab | 1.04 ± 0.09 b |
‘水晶柚3’Shuijingyou 3 | 1 349.62 ± 204.80 ab | 187.94 ± 10.11 a | 158.14 ± 7.81 ab | 1.19 ± 0.06 a |
试验材料 Plant material | 可溶性固形物/% Total soluble solid | 可滴定酸/% Titratable acid | 固酸比 Solidity-acid ratio | 维生素C/(mg · kg-1) Vitamin C |
‘东试早柚’Dongshi Zaoyou | 10.93 ± 0.19 a | 0.16 ± 0.02 b | 71.36 ± 9.49 a | 788.8 ± 1.32 a |
‘沙田柚’Shatianyou | 8.55 ± 0.70 b | 0.41 ± 0.18 a | 27.20 ± 15.82 b | 646.0 ± 8.47 b |
‘水晶柚1’Shuijingyou 1 | 9.44 ± 0.40 b | 0.32 ± 0.07 ab | 30.32 ± 5.44 b | 570.7 ± 4.57 b |
‘水晶柚2’Shuijingyou 2 | 8.95 ± 0.24 b | 0.18 ± 0.03 ab | 52.51 ± 10.48 b | 568.2 ± 2.71 b |
‘水晶柚3’Shuijingyou 3 | 8.88 ± 0.62 b | 0.25 ± 0.05 ab | 37.00 ± 5.35 b | 539.6 ± 6.22 b |
Fig. 5 Comparison of SNP density in different pummelo cultivars A,B and C:SNP density histograms of‘Dongshi Zaoyou’,‘Shuijingyou’and‘Shatianyou’pummelo,respectively;D:Chromosome;E,F and G:SNP density heat maps of‘Dongshi Zaoyou’,‘Shuijingyou’and‘Shatianyou’pummelo,respectively.
Fig. 7 The evolutionary trees of‘Dongshi Zaoyou’,‘Shatianyou’and‘Shuijingyou’pummelo The values in the graph indicate the branch length information,i.e. genetic variability;Tree scale denotes branch lengths with a genetic variance of 0.1 indication.
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