WGCNA Analysis of Differentially Expressed Genes in Floral Organs of Tea Germplasms with Ovary-glabrous and Ovary-trichome

doi:10.16420/j.issn.0513-353x.2021-1189

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 620-634.doi: 10.16420/j.issn.0513-353x.2021-1189

• Research Papers • Previous Articles Next Articles

WGCNA Analysis of Differentially Expressed Genes in Floral Organs of Tea Germplasms with Ovary-glabrous and Ovary-trichome

WANG Zehan¹, YU Wentao²^,^*(), WANG Pengjie¹, LIU Caiguo¹, FAN Xiaojing¹, GU Mengya¹, CAI Chunping², WANG Pan¹, YE Naixing¹^,^*()

¹College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou 350002，China
²Fujian Key Laboratory for Technology Research of Inspection and Quarantine，Technology Centre of Fuzhou Customs District P.R. China，Fuzhou 350001，China

Received:2022-10-19 Revised:2022-12-03 Online:2023-03-25 Published:2023-04-03
Contact: *（E-mail：ynxtea@126.com，wtyu@foxmail.com）

Abstract

Abstract:

In order to explore the molecular differences between the floral organs of two different types of tea germplasms，transcriptome sequencing analysis was performed on ovary-glabrous tea germplasms and ovary-trichome tea germplasm at their bud period and open period. The results showed that the differentially expressed genes between ovary-glabrous tea germplasms and ovary-trichome tea germplasm were 2 086-4 733. These differentially expressed genes involve in plant pathogen interaction， flavonoid biosynthesis and glutathione metabolism. Eighteen modules were identified by WGCNA. And three modules with the highest correlation with tea floral organ were screened out. By calculating the connectivity of genes in the module，the core genes in the network are excavated and annotated. The results showed that there were differences in resistance and synthesis of secondary metabolites between ovary-glabrous and ovary-trichome tea germplasms. The ovary-glabrous germplasm without trichome is susceptible to pathogen invasion and stress of adversity. It can improve its ability to cope with stress by regulating GST. The CsLTP may be a key gene regulating the trichome development of tea flower ovary.

Key words: Camellia sinensis, ovary-glabrous, ovary, ovarian-trichome, WGCNA, transcriptome

CLC Number:

S571.1

WANG Zehan, YU Wentao, WANG Pengjie, LIU Caiguo, FAN Xiaojing, GU Mengya, CAI Chunping, WANG Pan, YE Naixing. WGCNA Analysis of Differentially Expressed Genes in Floral Organs of Tea Germplasms with Ovary-glabrous and Ovary-trichome[J]. Acta Horticulturae Sinica, 2023, 50(3): 620-634.

Figures/Tables 15

References 44

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基因ID Gene ID	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
CsTGY12G0000254	ACCAAAGTTGGTGCGTTGAT	TCCACACTTCTTTCCAACAGG
CsTGY01G0001349	CACCGTGACAGCATTCATCT	GAAGCCGAGAGAGACACCAC
CsTGY03G0002199	TGCAGTGGTCTCAAACAAGC	AAGAGCAGGGCACTGAGAAA
CsTGY04G0000919	ACGGCATGTCTGATTGTCTG	CACCAGGAGTTGAGGGAGAA
CsTGY05G0002437	ACATCACGGGGAACTCTTTG	TGATGGGTGGAGTCTGAACA
CsTGY03G0000664	GCCTCTCCTACGTCACCAAC	CAGAGGGAGCAGAGAGATGG

基因ID Gene ID	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
CsTGY12G0000254	ACCAAAGTTGGTGCGTTGAT	TCCACACTTCTTTCCAACAGG
CsTGY01G0001349	CACCGTGACAGCATTCATCT	GAAGCCGAGAGAGACACCAC
CsTGY03G0002199	TGCAGTGGTCTCAAACAAGC	AAGAGCAGGGCACTGAGAAA
CsTGY04G0000919	ACGGCATGTCTGATTGTCTG	CACCAGGAGTTGAGGGAGAA
CsTGY05G0002437	ACATCACGGGGAACTCTTTG	TGATGGGTGGAGTCTGAACA
CsTGY03G0000664	GCCTCTCCTACGTCACCAAC	CAGAGGGAGCAGAGAGATGG

基因ID Gene ID	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
CsTGY14G0001328	ACTGGGTTGCATCCTCAAGA	TCGTTTCTGCGCGATTCAAG
CsTGY12G0000650	GCTGGTAAGATGGAGACCGA	TCTGTGGATCAGGGACATCCT
CsTGY02G0001254	ACGCACCTTCATTGGATGCT	CCACTGTGATCGGTGTCACT
CsTGY10G0000491	GCAGAGTGCTGATCATCGGA	AAGGTGGTTCGACCGGAATC
CsTGY09G0000731	GGACTAAGTTCGCCGAGGAG	TCCATCCAAGTGCAAGGTCC
CsTGY09G0000726	GGACTAAGTTCGCCGAGGAG	TCCATCCAAGTGCAAGGTCC

基因ID Gene ID	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
CsTGY14G0001328	ACTGGGTTGCATCCTCAAGA	TCGTTTCTGCGCGATTCAAG
CsTGY12G0000650	GCTGGTAAGATGGAGACCGA	TCTGTGGATCAGGGACATCCT
CsTGY02G0001254	ACGCACCTTCATTGGATGCT	CCACTGTGATCGGTGTCACT
CsTGY10G0000491	GCAGAGTGCTGATCATCGGA	AAGGTGGTTCGACCGGAATC
CsTGY09G0000731	GGACTAAGTTCGCCGAGGAG	TCCATCCAAGTGCAAGGTCC
CsTGY09G0000726	GGACTAAGTTCGCCGAGGAG	TCCATCCAAGTGCAAGGTCC

种质名称 Germplasm name	萼片数 Number of sepals	花瓣数 Number of petals	花柱长/cm Style length	花柱开裂数 Style dehiscence	子房茸毛 Ovarian trichome	雄蕊数 Number of stamens	花柄长/cm Pedicel length
云霄1号（茸房） Yunxiao 1（Ovary-trichome）	4.7 ± 0.5 b	5.7 ± 0.7 b	1.21 ± 0.14 a	3.0 ± 0.0 a	有Have	218.9 ± 24.7 a	0.44 ± 0.14 a
云霄2号（秃房） Yunxiao 2（Ovary-glabrous）	5.3 ± 0.7 a	6.1 ± 0.6 b	1.17 ± 0.13 a	3.0 ± 0.0 a	无Not have	224.2 ± 6.7 a	0.48 ± 0.06 a
云霄3号（秃房） Yunxiao 3（Ovary-glabrous）	4.9 ± 0.3 ab	6.8 ± 0.4 a	1.15 ± 0.11 a	3.0 ± 0.0 a	无Not have	218.9 ± 18.1 a	0.49 ± 0.09 a

WGCNA Analysis of Differentially Expressed Genes in Floral Organs of Tea Germplasms with Ovary-glabrous and Ovary-trichome

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种质名称 Germplasm name	样本ID Sample ID	过滤序列 Clean reads	过滤碱基 Clean bases	GC/%	质量值/% Q30	比对率/% Rate of mapped
云霄1号 Yunxiao 1	YX1a	22 925 163	6 863 327 433	44.83	92.82	81.26
	YX1b	21 032 668	6 301 007 194	45.11	94.24	87.78
云霄2号 Yunxiao 2	YX2a	22 121 647	6 615 662 648	45.12	93.49	77.57
	YX2b	21 364 673	6 395 708 936	44.96	93.04	88.16
云霄3号 Yunxiao 3	YX3a	22 463 254	6 721 197 850	44.74	93.12	88.11
	YX3b	21 843 812	6 536 443 013	45.00	93.32	87.58

秃房种质 Ovary-glabrous tea germplasms	花期 Florescence	差异表达基因数 Number of differentially expressed genes
秃房种质 Ovary-glabrous tea germplasms	花期 Florescence	总数Total	上调Up	下调Down
云霄2号Yunxiao 2	花苞期Bud period	2 540	1 359	1 181
	开放期Open period	2 086	1 128	958
云霄3号Yunxiao 3	花苞期Bud period	4 483	2 453	2 030
	开放期Open period	4 733	323	1 502

模块 Module	核心基因 Hub gene	基因功能描述 Gene description
灰色60	CsTGY15G0001792	蛋白酶抑制剂（KPI1）Kunitz protease inhibitor（KPI1）
Grey60	CsTGY05G0001055	糖转运蛋1Sugar transport protein 1-like
	CsTGY02G0002915	预测：茶树（-）-germacrened合成酶样 PREDICTED：Camellia sinensis (-)-germacrene D synthase-like
	CsTGY09G0000488	属于LTP基因家族Belongs to the plant LTP family
	CsTGY03G0000316	茶树枯草杆菌蛋白酶SBT1.7 Camellia sinensis subtilisin-like protease SBT1.7
	CsTGY10G0000634	预测：茶树功能未知基因LOC114317116 PREDICTED：Camellia sinensis uncharacterized LOC114317116
	CsTGY11G0002206	属于扩展蛋白家族Belongs to the expansin family
	CsTGY06G0003180	预测：山茶生长素反应蛋白SAUR21 PREDICTED：Camellia sinensis auxin-responsive protein SAUR21-like
	CsTGY14G0001328	BAG家族分子伴侣调节因子1-样异构体X2 BAG family molecular chaperone regulator 1-like isoform X2
	CsTGY02G0002875	F-box蛋白F-box protein
宝石绿	CsTGY11G0001375	功能未知蛋LOC114283154 Uncharacterized protein LOC114283154
Paleturquoise	CsTGY04G0002762	茶树果糖二磷酸醛缩酶6 Camellia sinensis fructose-bisphosphate aldolase 6
	CsTGY06G0001205	假设蛋白TEA_029793 Hypothetical protein TEA_029793
	CsTGY04G0001352	预测：可能是茶树硫酸盐转运体3.5 PREDICTED：Camellia sinensis probable sulfate transporter 3.5
	CsTGY12G0000569	茶树可能的过氧化酶5 Camellia sinensis probable peroxygenase 5
	CsTGY10G0001614	含有 WD 重复序列的蛋白质WD repeat-containing protein
	CsTGY03G0002931	谷胱甘肽S-转移酶Glutathione S-transferase F4-like
	CsTGY05G0002487	预测：茶树2-羟基异黄酮类脱水酶 PREDICTED：Camellia sinensis 2-hydroxyisoflavanone dehydratase-like
	CsTGY10G0001615	预测：茶树WD重复蛋白44 PREDICTED：Camellia sinensis WD repeat-containing protein 44-like
	CsTGY12G0000650	MLP蛋白28亚型X2MLP-like protein 28 isoform X2
仿古白4	CsTGY15G0001697	茶树MYB基因Camellia sinensis MYB mRNA
Antiquewhite 4	CsTGY03G0000487	属于CRISP家族Belongs to the CRISP family
	CsTGY13G0001142	茶树蛋白酶抑制剂2 Camellia sinensis kunitz trypsin inhibitor 2-like
	CsTGY12G0001632	属于扩展蛋白家族Belongs to the expansin family
	CsTGY08G0002347	预测：可能为茶树WRKY转录因子14 PREDICTED：Camellia sinensis probable WRKY transcription factor 14
	CsTGY04G0001579	茶树未知功能蛋白DDB Camellia sinensis putative uncharacterized protein DDB
	CsTGY11G0000895	预测：茶树锌指蛋白ZAT-12 PREDICTED：Camellia sinensis zinc finger protein ZAT12-like
	CsTGY08G0000509	预测：茶树富含半胱氨酸受体样蛋白激酶10 PREDICTED：Camellia sinensis cysteine-rich receptor-like protein kinase 10
	CsTGY06G0000838	茶树未知功能基因LOC114309800 Camellia sinensis uncharacterized LOC114309800
	CsTGY01G0003170	未知功能基因LOC114290224 Uncharacterized protein LOC114290224

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