ABC Transporter SlABCG23 Regulates Jasmonic Acid Signaling Pathway in Tomato

doi:10.16420/j.issn.0513-353x.2022-0160

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 559-568.doi: 10.16420/j.issn.0513-353x.2022-0160

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ABC Transporter SlABCG23 Regulates Jasmonic Acid Signaling Pathway in Tomato

LIU Yuhan, TAO Ning, WANG Qingguo, LI Qingqing^*()

College of Food Science and Technology，Shandong Agricultural University，Tai’an，Shandong 271018，China

Received:2022-11-20 Revised:2023-02-25 Online:2023-03-25 Published:2023-04-03
Contact: *（E-mail：anruihu001@163.com）

Abstract

Abstract:

The transport of jasmonic acid（JA）depends on the participation of transporters. ABC（ATP binding cassette）transporter is involved in the transport of various substances in plants. Four ABC transporters with high homology to the JA transporter in Arabidopsis thaliana are known to exist in tomato. Motif，protein conserved domain and transmembrane structure analysis were performed，finally，SlABCG23 was selected for preliminary function identification. It was speculated that SlABCG23 may be related to the regulation of JA signaling pathway. The overexpression vector of SlABCG23 was constructed，and three overexpression tomato plants were obtained by Agrobacterium infection. Phenotypic analysis showed that SlABCG23 overexpressed plants had enlarged flower organs，blocked pollen germination，abnormal seed development in fruits，following with decreased JA content in vivo. Therefore，it is speculated that SlABCG23 might be involved in regulating JA signaling pathway by affecting JA content.

Key words: tomato, jasmonic acid, transporter protein, SlABCG23

CLC Number:

S641.2

LIU Yuhan, TAO Ning, WANG Qingguo, LI Qingqing. ABC Transporter SlABCG23 Regulates Jasmonic Acid Signaling Pathway in Tomato[J]. Acta Horticulturae Sinica, 2023, 50(3): 559-568.

Figures/Tables 9

Table 1 The primers used in this study

用途 Function	引物名称 Primer name	序列（5′-3′） Sequence
全长扩增 Full length amplification	SlABCG23	F：GAGCTCATGTCTAGTCTTTGTGGAGGC R：GGTACCCCTCCTCTTATTTTTGCTTCCA
qRT-PCR	qSlABCG23	F：GCTTATCGCCGTTCCTCTTATG R：GGCTAGTCCAACTGACCAATATG
qRT-PCR	SlActin	F：TGTCCCTATTTACGAGGGTTATGC R：CAGTTAAATCACGACCAGCAAGAT
过表达植株鉴定Identification of overexpressing lines	OE-SlABCG23	F：GCACAATCCCACTATCCTTCGCA R：AGCAGAGTCGCCAAAGCGTTT

Fig. 1 Motif analysis

Fig. 2 Analysis of SlABCG23 protein conserved domains

Fig. 3 Analysis of SlABCG23 transmembrane domain

Fig. 4 The expression level of SlABCG23 in different tissues Different lowercase letters represent significant differences（P < 0.05），the same below.

Fig. 5 PCR confirmation of SlABCG23 overexpressing lines（OE1，OE6 and OE11）（A），RT-PCR（B）and qRT-PCR（C）

Fig. 6 The phenotype of SlABCG23 overexpressed plants（OE1，OE6 and OE11）flowers（A）,the length of flower（B）and fruits（C）

Fig. 7 Pollen morphology and germination of wild type（WT）and SlABCG23 overexpressed lines（OE1，OE6 and OE11）

Fig. 8 The JA content in flower organs of wild type（WT）and SlABCG23 overexpressed tomato plants（OE）

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ABC Transporter SlABCG23 Regulates Jasmonic Acid Signaling Pathway in Tomato

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