Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 534-548.doi: 10.16420/j.issn.0513-353x.2022-0003
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MAO Kexin1,2, AN Miao1, WANG Hairong1, WANG Shijin3, LÜ Wei2, GUO Yingtian1, LI Jian1,*(), LI Guotian1,*(
)
Received:
2022-09-03
Revised:
2022-12-30
Online:
2023-03-25
Published:
2023-04-03
Contact:
*(E-mail:lijian097597@163.com,ligt2008@163.com)
CLC Number:
MAO Kexin, AN Miao, WANG Hairong, WANG Shijin, LÜ Wei, GUO Yingtian, LI Jian, LI Guotian. Identification and Low Temperature Expression Analysis of MYB Transcription Factor Family in Kiwifruit[J]. Acta Horticulturae Sinica, 2023, 50(3): 534-548.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0003
基因Gene | 上游引物(5′-3′)Forward primer | 下游引物(5′-3′)Reverse primer |
---|---|---|
AcMYB200 | GAGATACCAGTGGCGGAGAG | CCCTGTATGCCGTTGCTTAT |
AcMYB61 | TCTTGGATGGTGTTGTTCCA | CCTGATCTGCTACGCTTTCC |
AcMYB63 | GAGGTGCTTAAGCCAACAGC | GTCCGACTTCCAAACTGCAT |
AcMYB56 | GTTCATAAGAGCGGGACCAA | GGAAGCATTTGGGTCAAAAA |
AcMYB71 | GAACCGGTGGTATTGAAACG | ACACGATCTCCACCTTTTGG |
AcMYB123 | CGACAAGTTCCTCGAAGCTC | TTTTGCGGGTAAGGATGAAC |
AcMYB210 | TGTGAATCCTCCACAAGCTG | ATGTGTCCCCGTTTTAGCAG |
AcMYB235 | CGACCTGAGCAAGAAGATCC | CTTCTGTGCATGGCTTCGTA |
Actin | GTGCTCAGTGGTGGTTCAA | GACGCTGTATTTCCTCTCAG |
Table 1 Primers in this study
基因Gene | 上游引物(5′-3′)Forward primer | 下游引物(5′-3′)Reverse primer |
---|---|---|
AcMYB200 | GAGATACCAGTGGCGGAGAG | CCCTGTATGCCGTTGCTTAT |
AcMYB61 | TCTTGGATGGTGTTGTTCCA | CCTGATCTGCTACGCTTTCC |
AcMYB63 | GAGGTGCTTAAGCCAACAGC | GTCCGACTTCCAAACTGCAT |
AcMYB56 | GTTCATAAGAGCGGGACCAA | GGAAGCATTTGGGTCAAAAA |
AcMYB71 | GAACCGGTGGTATTGAAACG | ACACGATCTCCACCTTTTGG |
AcMYB123 | CGACAAGTTCCTCGAAGCTC | TTTTGCGGGTAAGGATGAAC |
AcMYB210 | TGTGAATCCTCCACAAGCTG | ATGTGTCCCCGTTTTAGCAG |
AcMYB235 | CGACCTGAGCAAGAAGATCC | CTTCTGTGCATGGCTTCGTA |
Actin | GTGCTCAGTGGTGGTTCAA | GACGCTGTATTTCCTCTCAG |
Fig. 2 Protein isoelectric point and molecular weight of kiwifruit MYB family The number in parenthes indicates the number of MYB proteins which molecular weight exceeding 100 000 D.
Fig. 8 Expression analysis of eight kiwifruit MYB genes under low temperature treatment Different letters indicate that there are significant differences in genes at the level of 0.05 under different treatment times. *,** indicated significant difference between treatment and control at 0.05 and 0.01 levels,respectively.
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