Studies on the Antifungal Effect of Matrine on Colletotrichum Species on Tea Plant

doi:10.16420/j.issn.0513-353x.2021-1118

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 432-440.doi: 10.16420/j.issn.0513-353x.2021-1118

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Studies on the Antifungal Effect of Matrine on Colletotrichum Species on Tea Plant

CHENG Qinghua, ZHANG Zhipeng, WU Yanping, WAN Yuhe, CHEN Yingjuan^*()

National Demonstration Center for Experimental Food Science and Technology Education，Department of Tea Science，College of Food Science，Southwest University，Chongqing 400715，China

Received:2022-09-17 Revised:2022-12-02 Online:2023-02-25 Published:2023-03-06
Contact: *（E-mail：cyj8@swu.edu.cn）

Abstract

Abstract:

In order to explore the application prospect of matrine in the prevention and treatment of tea disease，the antifungal activity of matrine on five different Colletotrichum species including C. fioriniae，C. gloeosporioides，C. chongqingense，C. karstii and C. camelliae has been determined in this study. The results showed that matrine had significant inhibitory activity against five different Colletotrichum species. EC₅₀（concentration for 50% of maximal effect）of the five Colletotrichum species were 5.856，6.557，3.038，7.963 and 4.397 mg · mL^-1，and the antifungal activity of different species are significantly different. At low concentrations（1-8 mg · mL^-1），matrine has a better control effect on both C. chongqingense and C. camelliae. With the increase of the concentration，the inhibitory rates of the five Colletotrichum species were all above 95% at 22 mg · mL^-1. Microscopic examination showed that matrine had a significant effect on the mycelial and conidial growth of five Colletotrichum species. With the increase of matrine concentration，part of hyphae expanded irregularly，the number of conidia decreased and the conidia grew abnormally. Results of the biological activity of Colletotrichum species showed that the cell structure were destroyed，the cell permeability increased，and the cell oxidative stress response enhanced. The results of this study confirmed the antifungal activity of matrine against Colletotrichum species，and preliminarily revealed its antimicrobial mechanism by damaging cell membrane，which provided a theoretical basis for the prevention and control of tea brown blight disease caused by Colletotrichum.

Key words: tea plant, matrine, Colletotrichum, antifungal activity, mechanism, conidium, bioactivity

CLC Number:

S571.1

CHENG Qinghua, ZHANG Zhipeng, WU Yanping, WAN Yuhe, CHEN Yingjuan. Studies on the Antifungal Effect of Matrine on Colletotrichum Species on Tea Plant[J]. Acta Horticulturae Sinica, 2023, 50(2): 432-440.

Figures/Tables 7

Fig. 1 Growth inhibition rate of five Colletotrichum species at different concentrations of matrine

Table 1 Antifungal activity of matrine against five Colletotrichum species

病原菌种 Species	毒力回归方程 Toxic regression equation	R²	EC₅₀/（mg · mL^-1）
松针刺盘胞菌C. fioriniae	y = 2.636x + 2.977	0.869	5.856
胶胞炭疽菌C. gloeosporioides	y = 2.136x + 3.256	0.846	6.557
重庆炭疽菌C. chongqingense	y = 1.643x + 4.207	0.886	3.038
喀斯特炭疽菌C. karstii	y = 2.909x + 2.378	0.824	7.963
山茶炭疽菌C. camelliae	y = 2.569x + 3.349	0.917	4.397

Fig. 2 Colony growth of five Colletotrichum species at different concentrations of matrine

Fig. 3 Effect of matrine on mycelial growth of five Colletotrichum species

Fig. 4 Effect of matrine on the conidial growth of five Colletotrichum species

Fig. 5 Effect of matrine with different concentrations on the relative conductivity of five Colletotrichum species Different lowercase letters indicate significant differences among different treatments.

Fig. 6 Changes of MDA content of five Colletotrichum species after 22 mg · mL-1matrine treatment

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Studies on the Antifungal Effect of Matrine on Colletotrichum Species on Tea Plant

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