Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum

doi:10.16420/j.issn.0513-353x.2021-0806

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 170-176.doi: 10.16420/j.issn.0513-353x.2021-0806

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Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum

HU Jingyu¹, QUE Kaijuan¹, MIAO Tianli¹, WU Shaozheng², WANG Tiantian², ZHANG Lei¹, DONG Xian¹, JI Pengzhang¹, DONG Jiahong¹^,^*()

¹School of Chinese Materia Medica and Yunnan，Key Laboratory of Southern Medicinal Resource，Yunnan University of Chinese Medicine，Kunming 650500，China
²Biotechnology and Germplasm Resources Institute，Yunnan Academy of Agricultural Sciences，Kunming 650205，China

Received:2022-03-25 Revised:2022-07-11 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail:dongjhn@126.com）

Abstract

Abstract:

In 2020，Iris tectorum Maxim plants exhibiting chlorotic spot and necrotic ringspot symptoms were observed on campus in Yunnan University of Chinese Medicine in Kunming City，Yunnan Province. The typical virions of orthotospoviruses were observed by electron microscope. The systemic necrotic spots symptoms were found on the plants of Nicotiana tabacum and N. benthamiana inoculated mechanically with the sap of the symptomatic I.tectorum Maxim leaves. The inoculated leaves of Capsicum chinense Jacquin PI152225 showed the local lethal spots，whilst no systemic infection were detected. To further confirm the pathogen，RT-PCR were performed to amplify the NSs and N genes of tomato spotted wilt orthotospovirus（TSWV），respectively. Sequence analysis showed the obtained sequences had the highest identities（99.79% and 100%）with NSs and N genes of TSWV isolates infecting tomato and Tropaeolum majus in Yunnan Province. The results indicated TSWV infection causes the chlorotic spots and necrotic ringspots sympt，oms in I. tectorum.

Key words: Iris tectorum, tomato spotted wilt orthotospovirus, N gene, NSs gene

CLC Number:

S682.19

HU Jingyu, QUE Kaijuan, MIAO Tianli, WU Shaozheng, WANG Tiantian, ZHANG Lei, DONG Xian, JI Pengzhang, DONG Jiahong. Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum[J]. Acta Horticulturae Sinica, 2023, 50(1): 170-176.

Figures/Tables 3

Fig. 1 Symptoms of naturally diseased iris and viral particles under the electron microscope A. Chlorotic spot at early state of infection；B. Necrotic ringspot at late state of infection；C. Chlorotic and necrotic spots on the capsule；D. Local lethal spots on the inoculated leaves of Capsicum chinense Jacquin PI152225；E. Micrograph of virus particles.

Fig. 2 Amplification of N and NSs genes of TSWV-ITM isolate M:DL2000 DNA marker；1:Healthy control；2-5:Diseased iris samples.

Fig. 3 Phylogenetic tree based on the N protein aa sequences of TSWV-ITM isolate and other TSWV isolates

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Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum

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