Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Pepper Isolate in Qinghai Province

doi:10.16420/j.issn.0513-353x.2021-0756

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 161-169.doi: 10.16420/j.issn.0513-353x.2021-0756

• Research Notes • Previous Articles Next Articles

Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Pepper Isolate in Qinghai Province

LUO Hailin¹, YUAN Lei¹, WENG Hua¹, YAN Jiahui¹^,^*(), GUO Qingyun¹^,^*(), WANG Wenqing², MA Xinming³

¹Key Laboratory of Agricultural Integrated Pest Management，Scientific Observing and Experimental Station of Crop Pest in Xining，Ministry of Agriculture and Rural Affairs，Academy of Agriculture and Forestry Sciences，Qinghai University，Xining 810016，China
²Vegetable Technical Service Center of Datong County，Xining 810016，China
³Bureau of Agriculture and Rural & Science and Technology Affairs of Xunhua County，Haidong，Qinghai 810600，China

Received:2022-05-12 Revised:2022-08-13 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：15209787170@163.com，guoqingyunqh@163.com）

Abstract

Abstract:

An investigation on pepper virus was conducted. Samples collected in Xunhua County，Qinghai Province shown symptoms of plant wilt and leaf shrinkage，which were very similar to the symptoms induced by broad bean wilt virus 2（BBWV2）. To identify the possible virus associated with the disease，20 samples were collected and detected with universal primer and specific primer using RT-PCR

and 13 samples shown positive results when using a pair of degenerate primers for BBWV2. The results showed that 13 of 20 pepper samples in Xunhua County were confirmed to be infected with broad bean wilting virus 2. Further phylogenetic analysis based on the nucleotide sequence also suggested that the virus detected was clustered with BBWV2 and shared the highest sequence identities with the isolates from abroad，and relatively lower identities with the isolates of broad bean wilt virus 1（BBWV1）or Lamium mild mosaic virus（LMMV）. All these results showed that the virus infected pepper is an isolate of BBWV2.

Key words: pepper, broad bean wilt virus, whole genome

CLC Number:

S641.3

LUO Hailin, YUAN Lei, WENG Hua, YAN Jiahui, GUO Qingyun, WANG Wenqing, MA Xinming. Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Pepper Isolate in Qinghai Province[J]. Acta Horticulturae Sinica, 2023, 50(1): 161-169.

Figures/Tables 6

References 27

[1]	Frederick A M. 1995. Virus Taxonomy: classification and nomenclature of viruses. sixth report of the International Committee on Taxonomy of Viruses. Archives of Virology, 140:391-392.
[2]	Go A, Reiko T, Kappei K, Sekine K T. 2013. Establishment of an agroinoculation system for Broad bean wilt virus 2. Archives of Virology, 158 (7):1549-1554. doi: 10.1007/s00705-013-1625-7 URL
[3]	He Z, Dong Z Z, Qin L, Gan H. 2021. Phylodynamics and codon usage pattern analysis of Broad Bean Wilt Virus 2. Viruses, 13 (2):198. doi: 10.3390/v13020198 URL
[4]	Hong Jian, Xie Li, Zhang Zhong-kai, Zhou Xue-ping. 2021. Plant viruses in the new 15-rank taxonomic system of ICTV. Acta Phytopathologica Sinica, 51 (2):143-162. (in Chinese)
	洪健, 谢礼, 张仲凯, 周雪平. 2021. ICTV最新十五级分类阶元病毒分类系统中的植物病毒. 植物病理学报, 51 (2):143-162.
[5]	Kim M H, Kwak H R, Choi B, Kwon S J, Seo J K. 2021. Genetic plasticity in RNA2 is associated with pathogenic diversification of broad bean wilt virus 2. Virus Research, 304(prepublish):198533.
[6]	Kuang Yunbo, Chen Manzu, Lu Yirong, Chen Jing, Ye Zuyun. 2017. Detection of Turnip mosaic virus and Broad bean wilt virus in Pseudostellaria heterophylla by duplex RT-PCR. Acta Horticulturae Sinica, 44 (4):784-791. (in Chinese)
	匡云波, 陈满足, 陆伊荣, 陈晶, 叶祖云. 2017. 太子参芜菁花叶病毒和蚕豆萎蔫病毒的双重RT-PCR检测. 园艺学报, 44 (4):784-791.
[7]	Kwak H R, Kim M K, Lee Y J, Seo J K, Kim J S, Kim K H, Cha B, Choi H S. 2013a. Molecular characterization and variation of the Broad bean wilt virus 2 isolates based on analyses of complete genome sequences. The Plant Pathology Journal, 29 (4):397-409. doi: 10.5423/PPJ.OA.03.2013.0036 URL
[8]	Kwak H R, Kim M K, Nam M, Kim J S, Kim K H, Cha B, Choi H S. 2013b. Genetic compositions of Broad bean wilt virus 2 infecting red pepper in Korea. The Plant Pathology Journal, 29 (3):274-284. doi: 10.5423/PPJ.OA.12.2012.0190 URL
[9]	Kwak H R, Lee Y J, Kim J, Kim M K, Kim J S, Choi H S, Seo J K. 2016. A determinant of disease symptom severity is located in RNA2 of Broad bean wilt virus 2. Virus Research, 211:25-28. doi: 10.1016/j.virusres.2015.09.018 URL
[10]	Kwon S J, Cho I S, Yoon J Y, Chung B N. 2018. Incidence and occurrence pattern of viruses on peppers growing in fields in Korea. Research in Plant Disease, 24 (1):66-74. doi: 10.5423/RPD.2018.24.1.66 URL
[11]	Li Liang, Yang Cai-xia, Hou Qiu-shi, Wang Yun-zhu, Yu Mei-chun. 2020. Identification of broad bean wilt virus 2 on Chenopodium L. in Liaoning. Journal of Shenyang Agricultural University, 51 (4):446-453. (in Chinese)
	李梁, 杨彩霞, 侯秋实, 王筠竹, 于美春. 2020. 蚕豆萎蔫病毒2号辽宁藜分离物的鉴定. 沈阳农业大学学报, 51 (4):446-453.
[12]	Li Quan-hui, Han Rui, Li Zhi-cheng, Chen Lai-sheng. 2018. The production situation and development countermeasures of chili pepper in Qinghai Province. Chinese Horticulture Abstracts, 34 (6):60-61,124. (in Chinese)
	李全辉, 韩睿, 李志成, 陈来生. 2018. 青海线辣椒生产状况及发展对策. 中国园艺文摘, 34 (6):60-61,124.
[13]	Li Ting-fang, Wu Shu-hua, Zhao Wen-hao, Ji Ying-hua, Zhou Yi-jun, Guo Qing-yun. 2017. Molecular detection of mild mottle virus isolated from pepper in Haidong,Qinghai Province. Jiangsu Journal of Agricultural Sciences, 33 (4):958-960. (in Chinese)
	李廷芳, 吴淑华, 赵文浩, 季英华, 周益军, 郭青云. 2017. 青海海东设施辣椒轻斑驳病毒的分子检测. 江苏农业学报, 33 (4):958-960.
[14]	Liu Yong, Li Fan, Li Yue-yue, Zhang Song-bai, Gao Xi-wu, Xie Yan, Yan Fei, Zhang An-sheng, Dai Liang-ying, Cheng Zhao-bang, Ding Ming, Niu Yan-bing, Wang Sheng-ji, Che Hai-yan, Jiang Tong, Shi Xiao-bin, He Zi-fu, Wu Yun-feng, Zhang De-yong, Qing Ling, Yan Wan-rong, Yang Xue-hui, Tang Ya-fei, Zheng Hong-ying, Tang Qian-jun, Zhang Song-bai, Zhang Dong-fang, Cai Li, Tao Xiao-rong. 2019. Identification,distribution and occurrence of viruses in the main vegetables of China. Scientia Agricultura Sinica, 52 (2):239-261. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.02.005
	刘勇, 李凡, 李月月, 张松柏, 高希武, 谢艳, 燕飞, 张安盛, 戴良英, 程兆榜, 丁铭, 牛颜冰, 王升吉, 车海彦, 江彤, 史晓斌, 何自福, 吴云锋, 张德咏, 青玲, 严婉荣, 杨学辉, 汤亚飞, 郑红英, 唐前君, 章松柏, 章东方, 蔡丽, 陶小荣. 2019. 侵染我国主要蔬菜作物的病毒种类、分布与发生趋势. 中国农业科学, 52 (2):239-261. doi: 10.3864/j.issn.0578-1752.2019.02.005
[15]	Niu Yan-bing, Zhao Xin, Zhao Hui-qi, Zhang Xi-mei. 2014. Identification and analysis of complete genomic sequence of broad bean wilt virus 2 shanxi isolate. Chinese Journal of Biochemistry and Molecular Biology, 30 (9):927-932. (in Chinese)
	牛颜冰, 赵欣, 赵慧琪, 张西梅. 2014. 蚕豆萎蔫病毒2号山西分离株的全基因组序列测定与分析. 中国生物化学与分子生物学报, 30 (9):927-932.
[16]	Qi Y, Zhou X, Li D. 2000. Complete nucleotide sequence and infectious cDNA clone of the RNA1 of a Chinese isolate of Broad bean wilt virus 2. Virus Genes, 20 (3):201-207. pmid: 10949946
[17]	Rosa M F, Inmaculada F, Pedro M, José G, Luis R. 2011. Genetic variation and evolutionary analysis of Broad bean wilt virus 2. Archives of Virology, 156 (8):1445-1450. doi: 10.1007/s00705-011-0990-3 URL
[18]	Stubbs L L. 1947. A destructive vascular wilt disease of broad bean (Vicia faba L.) in Australia. Journal of the Department of Agriculture, 46:323-332.
[19]	Sun Guo-sheng, Ma Zhi-hu, Dai Zhong-liang, Mao Zhong-liang, Sun Chun-qing, Pan Yue-ping. 2016. Comparison of three methods on total RNA extraction from the root of Capsicum annuum L. China Cucurbits and Vegetables, 29 (1):8-10. (in Chinese)
	孙国胜, 马志虎, 戴忠良, 毛忠良, 孙春青, 潘跃平. 2016. 3种方法提取辣椒根总RNA的效果比较. 中国瓜菜, 29 (1):8-10.
[20]	Wang Bao-xia, Qi Yong-hong, Xin min, Cui Li-yan, Wang De-fu, Niu Yan-bing. 2019. Identification and analysis of genomic S of Broad been wilt virus 2 from Isatidis Radx. Chinese Journal of Biochemistry and Molecular Biology, 35 (3):333-340. (in Chinese)
	王宝霞, 齐永红, 辛敏, 崔丽艳, 王德富, 牛颜冰. 2019. 蚕豆萎蔫病毒2号板蓝根分离株全基因组序列测定与分析. 中国生物化学与分子生物学报, 35 (3):333-340.
[21]	Wang Shao-li, Tan Wei-ping, Yang Yuan-yuan, Dai Hui-jie, Sun Xiao-hui, Qiao Ning, Zhu Xiao-ping. 2017. Molecular detection and identification of main viruses on pepper in Shandong Province. Scientia Agricultura Sinica, 50 (14):2728-2738. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.14.009
	王少立, 谭玮萍, 杨园园, 代惠洁, 孙晓辉, 乔宁, 竺晓平. 2017. 山东省辣椒主要病毒种类的分子检测与鉴定. 中国农业科学, 50 (14):2728-2738. doi: 10.3864/j.issn.0578-1752.2017.14.009
[22]	Wu Shu-hua, Tu Li-qin, Xian Wen-rong, Yan Jia-hui, Gao Dan-na, Ji ying, Tao Xiao-rong, Zhou Yi-jun, Guo Qing-yun, Ji Ying-hua. 2020. Detection and identification of Tomato spotted wilt tospovirus on pepper from Qinghai Province. Acta Horticulturae Sinica, 47 (7):1391-1400. (in Chinese)
	吴淑华, 涂丽琴, 咸文荣, 闫佳会, 高丹娜, 吉颖, 陶小荣, 周益军, 郭青云, 季英华. 2020. 青海辣椒上番茄斑萎病毒检测及鉴定. 园艺学报, 47 (7):1391-1400.
[23]	Xi Zhong-xing, Xu Shao-hua, Mang Ke-qiang. 1982. An isolate causing systemic necrosis mosaic on cowpea in Peking suburb. Acta Phytopathologica Sinica, 12 (4):40-44. (in Chinese)
	奚仲兴, 徐绍华, 莽克强. 1982. 引起北京豇豆系统坏死和花叶的一个分离物——Ⅰ.寄主范围、提纯和超薄切片. 植物病理学报, 12 (4):40-44.
[24]	Yan Dan-kan, Zheng Hong-ying, Zhang Hai-shan, Shen Yan, Gu Jiang-tao, Zhang Dong-fang, Yan Fei. 2018. Identification and analysis of complete genomic sequence of Broad bean wilt virus 2 Anhui isolate. Plant Protection, 44 (1):67-73. (in Chinese)
	严丹侃, 郑红英, 张海珊, 沈艳, 顾江涛, 章东方, 燕飞. 2018. 蚕豆萎蔫病毒2号安徽分离物全基因组序列测定与分析. 植物保护, 44 (1):67-73.
[25]	Yoon J Y, Ki H R, Choi S K, Gug S C, Kwon S B, Jin W P, Peter P. 2012. Transient expression of homologous hairpin RNA interferes with Broad bean wilt virus 2 infection in nicotiana benthamiana. Research in Plant Disease, 18 (4):268-276. doi: 10.5423/RPD.2012.18.4.268 URL
[26]	Zhang Mengyan, Zhang Zunping, Ren Fang, Hu Guojun, Fan Xudong, Dong Yafeng. 2020. Establishment and application of a real-time fluorescent quantitative RT-PCR for detection of Grapevine fabavirus. Acta Horticulturae Sinica, 47 (1):187-194. (in Chinese)
	张梦妍, 张尊平, 任芳, 胡国君, 范旭东, 董雅凤. 2020. 葡萄蚕豆萎蔫病毒实时荧光定量RT-PCR检测方法及应用. 园艺学报, 47 (1):187-194.
[27]	Zhou Xue-ping, Qi Yi-jun, Li Fan, Huang Xiao-zao, Li De-bao. 2001. Complete nucleotide sequence and possible genomic expression strategy of a Chinese isolate of Broad bean wilt virus. Acta Biochimica et Biophysica Sinica, 33 (1):46-52. (in Chinese)
	周雪平, 戚益军, 李凡, 黄晓璪, 李德葆. 2001. 蚕豆萎蔫病毒2中国分离物全基因组结构及可能的基因表达方式. 生物化学与生物物理学报, 33 (1):46-52.

病毒序列 Virus sequence	引物名称 Name	引物序列（5′-3′） Sequence	退火温度/℃ T_m	片段大小/bp Length	文献 Reference
BBWV2	BBWVdF	AGRTATATGCTTGGGCAAGCGCATG	60	490	刘勇等， 2019
	BBWVdR	CATGAACATTCCCCATCTCCACGTG			刘勇等， 2019
RNA1	BBWV2 1-F	AAACAAACAGCTTTCGTTCCG	54	1 080	Kwak et 2013b
	BBWV2 1-R	CTGAAATAGCTGCCATAGCAG
	BBWV2 1-F2	TTTGCAGCGTGGATGCC	55	1 075
	BBWV2 1-R2	CTTGTAGCCTGACCAAAAAGG
	BBWV2 1-F3	SGCAGATTGGTTATSWGAR	53	880
	BBWV2 1-R3	ACCGTGAGRCCCACWAR
	BBWV2 1-F4	MGRCGAAGRCACGCAGTRRT	57	1 083
	BBWV2 1-R4	AAGCACACTTGTGTYCTMACAT
	BBWV2 1-F5	CAAAGTGATRTKCCTAAYC	48	1 561
	BBWV2 1-R5	CCRTCAAAYCTGCTGTAA
	BBWV2 1-F6	CGSCTWGATCGTGGTCC	54	1 766
	BBWV2 1-R6	TTCCCTCACTACTGAAATTTACTC
RNA2	BBWV2 2-1F	CAAACAGCTTTCGTTCCGAA	52	927	Kwak et 2013b
	BBWV2 2-1R	AAGCCATCTGCCACCAATTC
	BBWY2 2-1d	CGCATA(G)GTC(T)TCTT(C)GTA(G)GCA(T)GA	55	900
	BBWV2 2-2F	GCCATGTCAGGTGCTGGA
	BBWV2 2-2U	TACAAT(C)TTG(A)CCTGGG(A)CCTAGC	56	1 119
	BBMV2 2-2r	CATGTTCCCAACCTTTTCAATGT
	BBWV2 2-3U	ATTT(C)TA(G)GATTTTACT(C)CTC(T)ATGAG	55	1 163
	BBWV2 2-3d	AGGTCATGGAACCCATTTTAATGG

病毒序列 Virus sequence	引物名称 Name	引物序列（5′-3′） Sequence	退火温度/℃ T_m	片段大小/bp Length	文献 Reference
BBWV2	BBWVdF	AGRTATATGCTTGGGCAAGCGCATG	60	490	刘勇等， 2019
	BBWVdR	CATGAACATTCCCCATCTCCACGTG			刘勇等， 2019
RNA1	BBWV2 1-F	AAACAAACAGCTTTCGTTCCG	54	1 080	Kwak et 2013b
	BBWV2 1-R	CTGAAATAGCTGCCATAGCAG
	BBWV2 1-F2	TTTGCAGCGTGGATGCC	55	1 075
	BBWV2 1-R2	CTTGTAGCCTGACCAAAAAGG
	BBWV2 1-F3	SGCAGATTGGTTATSWGAR	53	880
	BBWV2 1-R3	ACCGTGAGRCCCACWAR
	BBWV2 1-F4	MGRCGAAGRCACGCAGTRRT	57	1 083
	BBWV2 1-R4	AAGCACACTTGTGTYCTMACAT
	BBWV2 1-F5	CAAAGTGATRTKCCTAAYC	48	1 561
	BBWV2 1-R5	CCRTCAAAYCTGCTGTAA
	BBWV2 1-F6	CGSCTWGATCGTGGTCC	54	1 766
	BBWV2 1-R6	TTCCCTCACTACTGAAATTTACTC
RNA2	BBWV2 2-1F	CAAACAGCTTTCGTTCCGAA	52	927	Kwak et 2013b
	BBWV2 2-1R	AAGCCATCTGCCACCAATTC
	BBWY2 2-1d	CGCATA(G)GTC(T)TCTT(C)GTA(G)GCA(T)GA	55	900
	BBWV2 2-2F	GCCATGTCAGGTGCTGGA
	BBWV2 2-2U	TACAAT(C)TTG(A)CCTGGG(A)CCTAGC	56	1 119
	BBMV2 2-2r	CATGTTCCCAACCTTTTCAATGT
	BBWV2 2-3U	ATTT(C)TA(G)GATTTTACT(C)CTC(T)ATGAG	55	1 163
	BBWV2 2-3d	AGGTCATGGAACCCATTTTAATGG

国家 Country	寄主 Host	分离物 Isolate	RNA1		RNA2
国家 Country	寄主 Host	分离物 Isolate	核苷酸/% Nucleotide	氨基酸/% Amion acid	核苷酸/% Nucleotide	氨基酸/% Amion acid
韩国Korea	蚕豆 Vicia faba	BBWV2-BB9	100	89.92	100	84.34
	菠菜 Spinacia oleracea	BBWV2-SP2	100	90.02	100	84.13
		BBWV2-SP1	100	90.00	100	84.19
		BBWV2-SP	100	89.97	100	84.72
	牛膝 Achyranthes bidentata Blume	BBWV2-AB1	100	90.24	100	86.97
	辣椒 Capsicum annuum	BBWV2-RP2	100	90.24	100	84.04
	辣椒	BBWV2-PAP1	100	89.95	100	84.80
	C. annuum var. gulosum	BBWV2-PAP3	100	91.48	100	84.91
	薯蓣 Dioscorea oppositifolia	BBWV2-BR1	99	77.44	99	87.16
中国China	蚕豆 Vicia faba	BBWV2-B935	99	77.58	100	84.51
	薯蓣 Dioscorea opposita	BBWV2-SG1	99	77.32	100	87.09
	辣椒 Capsicum annuum L.	BBWV2-Ca	99	77.36	99	82.41
	番茄 Lycopersicon esculentum	BBWV2-XJ14-3	99	77.50	100	87.18
日本Japan	三花龙胆 Gentiana triflora	BBWV2-Ty	99	77.08	99	88.61
	蚕豆 Vicia faba	BBWV2-MB7	99	78.03	100	85.90
西班牙Spain	辣椒 Capsicum annuum	BBWV1-Ben	74	65.68	62	64.99
	蚕豆 Vicia faba	BBWV1-PBenR1	74	66.01	62	65.16
英国 United Kingdom	块根落葵 Ullucus tuberosus	BBWV1-UL1	73	65.88	52	66.61

国家 Country	寄主 Host	分离物 Isolate	RNA1		RNA2
国家 Country	寄主 Host	分离物 Isolate	核苷酸/% Nucleotide	氨基酸/% Amion acid	核苷酸/% Nucleotide	氨基酸/% Amion acid
韩国Korea	蚕豆 Vicia faba	BBWV2-BB9	100	89.92	100	84.34
	菠菜 Spinacia oleracea	BBWV2-SP2	100	90.02	100	84.13
		BBWV2-SP1	100	90.00	100	84.19
		BBWV2-SP	100	89.97	100	84.72
	牛膝 Achyranthes bidentata Blume	BBWV2-AB1	100	90.24	100	86.97
	辣椒 Capsicum annuum	BBWV2-RP2	100	90.24	100	84.04
	辣椒	BBWV2-PAP1	100	89.95	100	84.80
	C. annuum var. gulosum	BBWV2-PAP3	100	91.48	100	84.91
	薯蓣 Dioscorea oppositifolia	BBWV2-BR1	99	77.44	99	87.16
中国China	蚕豆 Vicia faba	BBWV2-B935	99	77.58	100	84.51
	薯蓣 Dioscorea opposita	BBWV2-SG1	99	77.32	100	87.09
	辣椒 Capsicum annuum L.	BBWV2-Ca	99	77.36	99	82.41
	番茄 Lycopersicon esculentum	BBWV2-XJ14-3	99	77.50	100	87.18
日本Japan	三花龙胆 Gentiana triflora	BBWV2-Ty	99	77.08	99	88.61
	蚕豆 Vicia faba	BBWV2-MB7	99	78.03	100	85.90
西班牙Spain	辣椒 Capsicum annuum	BBWV1-Ben	74	65.68	62	64.99
	蚕豆 Vicia faba	BBWV1-PBenR1	74	66.01	62	65.16
英国 United Kingdom	块根落葵 Ullucus tuberosus	BBWV1-UL1	73	65.88	52	66.61

Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Pepper Isolate in Qinghai Province

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 27

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CAO Yacong, ZHANG Zhenghai, YU Hailong, FENG Xigang, ZHANG Baoxi, and WANG Lihao. A New Spiral Pepper Cultivar‘Zhongjiao 409’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 111-112.
[2]	CAO Yacong, ZHANG Zhenghai, YU Hailong, FENG Xigang, ZHANG Baoxi, and WANG Lihao. A New Disease-resistant Spiral Hot Pepper Cultivar‘Zhongjiao 209’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 113-114.
[3]	WANG Yongping, HU Mingwen, ZHU Wenchao, LIAO Fangfang, BAI Liwei, and GAO Gang. A New Pepper Cultivar‘Hongla 3’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 115-116.
[4]	Liu Shujun, Hui Chengzhang, Zhao Lili, Sun Yongsheng, and Liu Aiqun . A New Mid-early Ripening Pepper Cultivar‘Meroka’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 117-118.
[5]	WANG Fei, LI Ning, YIN Yanxu, GAO Shenghua, XU Kai, and YAO Minghua. A New Pepper Cultivar‘Ejiao Hongyuanshuai’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 119-120.
[6]	LI Ning, YIN Yanxu, GAO Shenghua, XU Kai, WANG Fei, and YAO Minghua. A New Pepper Cultivar‘Jinxiuhong 117’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 121-122.
[7]	FANG Rong, CHEN Xuejun, ZHOU Kunhua, YUAN Xinjie, LEI Gang, and HUANG Yueqin. A New Pepper Cultivar‘Ganjiao 18’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 75-76.
[8]	DONG Sangjie, GE Shibei, LI Lan, HE Liqun, FAN Feijun, QI Zhenyu, YU Jingquan, ZHOU Yanhong. Effects of Supplemental Lighting on Growth,Root Colonization by Arbuscular Mycorrhizal Fungi and Phosphorus Uptake in Pepper Seedlings [J]. Acta Horticulturae Sinica, 2022, 49(8): 1699-1712.
[9]	LI Ying, WANG Hengming, XU Xiaowan, XU Xiaomei, HUANG Zhiwen. A New Pepper Cultivar‘Yuejiao 8’ [J]. Acta Horticulturae Sinica, 2022, 49(8): 1837-1838.
[10]	ZOU Xuexiao, ZHU Fan. Origin,Evolution and Cultivation History of the Pepper [J]. Acta Horticulturae Sinica, 2022, 49(6): 1371-1381.
[11]	FU Hongfei, NIE Zhixing, CHEN Jianying. A New Pepper‘Hangjiao 12’ [J]. Acta Horticulturae Sinica, 2022, 49(6): 1395-1396.
[12]	GONG Mingxia, ZHAO Hu, WANG Meng, WU Xing, ZHAO Zengjing, HE Zhi, HUANG Jinmei, MENG Shengde, WANG Risheng. Identification of Viruses Infecting Peppers in Guangxi by Small RNA Deep Sequencing and RT-PCR [J]. Acta Horticulturae Sinica, 2022, 49(5): 1060-1072.
[13]	ZHANG Shicai, LI Yifei, WANG Chunping, YANG Xiaomiao, HUANG Qizhong, HUANG Renzhong. Resistance Identification and Evaluation of Pepper Germplasm to Colletotrichum acutatum [J]. Acta Horticulturae Sinica, 2022, 49(4): 885-892.
[14]	ZHOU Chenping, YANG Min, GUO Jinju, KUANG Ruibin, YANG Hu, HUANG Bingxiong, WEI Yuerong. Dynamic Changes in DNA Methylome and Transcriptome Patterns During Papaya Fruit Ripening [J]. Acta Horticulturae Sinica, 2022, 49(3): 519-532.
[15]	ZOU Xuexiao, HU Bowen, XIONG Cheng, DAI Xiongze, LIU Feng, OU Lijun, YANG Bozhi, LIU Zhoubin, SUO Huan, XU Hao, ZHU Fan, and YUAN Fang. Review and Prospects of Pepper Breeding for the Past 60 Years in China [J]. Acta Horticulturae Sinica, 2022, 49(10): 2099-2118.