Isolation，Identification and Analysis of Biocontrol Ability of Biocontrol Strain TL1 Against Tomato Botrytis cinerea

doi:10.16420/j.issn.0513-353x.2021-0924

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 79-90.doi: 10.16420/j.issn.0513-353x.2021-0924

• Research Papers • Previous Articles Next Articles

Isolation，Identification and Analysis of Biocontrol Ability of Biocontrol Strain TL1 Against Tomato Botrytis cinerea

SHI Hongli¹, LI La¹, GUO Cuimei¹, YU Tingting¹, JIAN Wei¹^,^*(), YANG Xingyong¹^,²^,^*()

¹College of Life Science，Chongqing Normal University，Chongqing 401331，China
²School of Pharmacy Chengdu University，Chengdu 601106，China

Received:2022-06-07 Revised:2022-07-27 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：jianwei7956@163.com，yangxy94@swu.edu.cn）

Abstract

Abstract:

In order to screen the antagonistic strain against Botrytis cinerea，a TL1 strain with good antagonistic effect was isolated from the soil of Tibet Plateau by plate confrontation method. The results of morphological observation，physiological and biochemical analysis and molecular biological identification showed that TL1 strain was Paenibacillus maysiensis，which could fix nitrogen and dissolving phosphorus. In addition，TL1 strain could significantly inhibit the mycelial growth of Botrytis cinerea on the plate，and the inhibition rate was 74.32%. It was further found that the volatile substances produced by TL1 strain showed significant inhibition activity. Plate inhibition test showed that TL1 strain had significant inhibition activity against six pathogenic fungi，such as brown spot. Among them，the inhibition rate of Alternaria alternata was the highest（73.48%），and the inhibition rate of Corynespora cassiicola was the lowest （57.37%）. Physiological experiments showed that TL1 strain could colonize tomato fruit and inhibit the decay caused by gray mold，and TL1 strain could significantly improve seed germination rate and promote the growth of tomato seedlings.

Key words: tomato, Botrytis cinerea, Paenibacillus, biocontrol, mechanism, inhibition

CLC Number:

S641.2

SHI Hongli, LI La, GUO Cuimei, YU Tingting, JIAN Wei, YANG Xingyong. Isolation，Identification and Analysis of Biocontrol Ability of Biocontrol Strain TL1 Against Tomato Botrytis cinerea[J]. Acta Horticulturae Sinica, 2023, 50(1): 79-90.

Figures/Tables 9

References 45

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指标 Index	特征 Characteristic	指标 Index	特征 Characteristic
接触酶实验 Contact enzyme test	+	葡萄糖 Glucose	-
甲基红（M-R）实验 Methyl red（M-R）experiment	+	麦芽糖 Maltose	+
糖类发酵实验 Saccharide fermentation experiment	-	半乳糖 Galactose	+
淀粉水解 Starch hydrolysis	-	甘露糖 Mannose	+
产纤维素酶实验 Cellulase production experiment	+	棉籽糖 Cottonseed sugar	-
硫化氢实验 Hydrogen sulfide experiment	+	D-核糖 D-ribose	-
固氮实验 Nitrogen fixation experiment	+	L-苏氨酸 L-threonine	+
溶磷实验 Soluble phosphorus experiment	+	L-天冬氨酸 L-aspartic acid	-
解钾实验 Potassium dissolving experiment	-	谷氨酸 Glutamate	+
产铁载体实验 Experiment of producing iron carrier	-	丝氨酸 Serine	+
明胶液化 Gelatin liquefaction	+	肌酸 Creatine	-
产蛋白酶 Protease production	+	L-天冬酰胺 L-asparagine	-

指标 Index	特征 Characteristic	指标 Index	特征 Characteristic
接触酶实验 Contact enzyme test	+	葡萄糖 Glucose	-
甲基红（M-R）实验 Methyl red（M-R）experiment	+	麦芽糖 Maltose	+
糖类发酵实验 Saccharide fermentation experiment	-	半乳糖 Galactose	+
淀粉水解 Starch hydrolysis	-	甘露糖 Mannose	+
产纤维素酶实验 Cellulase production experiment	+	棉籽糖 Cottonseed sugar	-
硫化氢实验 Hydrogen sulfide experiment	+	D-核糖 D-ribose	-
固氮实验 Nitrogen fixation experiment	+	L-苏氨酸 L-threonine	+
溶磷实验 Soluble phosphorus experiment	+	L-天冬氨酸 L-aspartic acid	-
解钾实验 Potassium dissolving experiment	-	谷氨酸 Glutamate	+
产铁载体实验 Experiment of producing iron carrier	-	丝氨酸 Serine	+
明胶液化 Gelatin liquefaction	+	肌酸 Creatine	-
产蛋白酶 Protease production	+	L-天冬酰胺 L-asparagine	-

病原真菌Pathogenic fungi	抑制率/% Inhibition	病原真菌Pathogenic fungi	抑制率/% Inhibition
黄瓜褐斑病菌Corynespora cassiicola	57.37 ± 0.96 ef	花生黑斑病菌Cercospora personatum	63.17 ± 1.35 c
烟草赤星病菌Alternaria alternata	73.48 ± 1.28 a	油菜黑斑病菌Alternaria brassicae	61.97 ± 1.48 cd
花生褐斑病菌Cercospora arachidicola	58.64 ± 1.41 e	恶苗病菌Fusarium moniliforme	68.14 ± 2.01 b

病原真菌Pathogenic fungi	抑制率/% Inhibition	病原真菌Pathogenic fungi	抑制率/% Inhibition
黄瓜褐斑病菌Corynespora cassiicola	57.37 ± 0.96 ef	花生黑斑病菌Cercospora personatum	63.17 ± 1.35 c
烟草赤星病菌Alternaria alternata	73.48 ± 1.28 a	油菜黑斑病菌Alternaria brassicae	61.97 ± 1.48 cd
花生褐斑病菌Cercospora arachidicola	58.64 ± 1.41 e	恶苗病菌Fusarium moniliforme	68.14 ± 2.01 b

处理 Treatment	株高/cm Plant height	出芽时间/d Germination time	根长/cm Root length	芽长/cm Sending length	根芽比 Root length/Sending length	发芽率/% Germination rate
对照Control	8.67 ± 1.24 a	3	4.25 ± 0.43 a	3.30 ± 0.23 a	1.29 ± 0.13 a	75.34 ± 1.92 a
TL1	12.73 ± 0.68 b	3	6.45 ± 0.38 b	3.80 ± 0.31 a	1.69 ± 0.27 b	93.15 ± 2.16 b

Isolation，Identification and Analysis of Biocontrol Ability of Biocontrol Strain TL1 Against Tomato Botrytis cinerea

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