Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit

doi:10.16420/j.issn.0513-353x.2021-1086

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 53-64.doi: 10.16420/j.issn.0513-353x.2021-1086

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Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit

YUAN Xin¹, XU Yunhe¹, ZHANG Yupei¹, SHAN Nan¹, CHEN Chuying¹, WAN Chunpeng¹, KAI Wenbin¹, ZHAI Xiawan¹, CHEN Jinyin¹^,²^,^*(), GAN Zengyu¹^,^*()

¹Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables，College of Agronomy，Jiangxi Agricultural University，Nanchang 330045，China
²College of Materials and Chemical Engineering，Pingxiang University，Pingxiang，Jiangxi 330075，China

Received:2022-09-16 Revised:2022-11-30 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：jinyinchen@126.com，ganzy@jxau.edu.cn）

Abstract

Abstract:

The effect of ABA on IAA metabolism during postharvest ripening of‘Hongyang’kiwifruit were studied. The results showed that exogenous ABA treatment could accelerate the degradation of free IAA，increase the content of IAA-Asp，and significantly promote the expression of IAA degradation related gene AcGH3.1. Five ABA-responsive binding factor genes（AcAREB1-AcAREB5）were isolated and identified from kiwifruit genome database. qRT-PCR showed that all five AcAREB genes could respond to ABA during postharvest ripening. Yeast one-hybrid results showed that AcAREB1 could directly bind to the AcGH3.1 gene promoter. Subcellular localization assay showed that AcAREB1 was located on the nucleus. Yeast self-activation experiments proved that AcAREB1 was a transcription factor with transcriptional activation activity. These results indicate that AcAREB1 can regulate the expression of AcGH3.1 gene corresponding to ABA during the ripening process of kiwifruit，thereby promoting the degradation of IAA，and finally leading to fruit softening and ripening.

Key words: kiwifruit, postharvest ripening, AcAREB1, AcGH3.1, promoter, ABA, IAA

CLC Number:

S663.4

YUAN Xin, XU Yunhe, ZHANG Yupei, SHAN Nan, CHEN Chuying, WAN Chunpeng, KAI Wenbin, ZHAI Xiawan, CHEN Jinyin, GAN Zengyu. Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit[J]. Acta Horticulturae Sinica, 2023, 50(1): 53-64.

Figures/Tables 11

References 35

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基因 Gene	上游序列（5′-3′）Forward sequence	下游序列（5′-3′）Reverse sequence
AcGH3.1	AGGGTCTGTACTTCCTGTTCG	TGTAGACGTTGTAGGGATCG
AcAREB1	GCAACAACAACCACCTTCAA	GTTCCCAAACCAATCATTCC
AcAREB2	GGGGCGGCAGTAGTGAACCA	AGTGTCAGACCAGGCGTAAT
AcAREB3	GGTCAGCGCACAGGATTGAG	AGCCAGATTTGATTGCTTGG
AcAREB4	ACGGATAGGAATAGTGCTGG	TAAATCACCGAAGAAGCCAC
AcAREB5	ATGGCTTCTTCGGTGATTTA	CTGCTGCTGTGATGATCTAAC
GUS	CACAAACCGTTCTACTTTACTGG	GCGTAAGGGTAATGCGAGGT
AcActin	TGGAATGGAAGCTGCAGGA	CACCACTGAGCACAATGTTGC

基因 Gene	上游序列（5′-3′）Forward sequence	下游序列（5′-3′）Reverse sequence
AcGH3.1	AGGGTCTGTACTTCCTGTTCG	TGTAGACGTTGTAGGGATCG
AcAREB1	GCAACAACAACCACCTTCAA	GTTCCCAAACCAATCATTCC
AcAREB2	GGGGCGGCAGTAGTGAACCA	AGTGTCAGACCAGGCGTAAT
AcAREB3	GGTCAGCGCACAGGATTGAG	AGCCAGATTTGATTGCTTGG
AcAREB4	ACGGATAGGAATAGTGCTGG	TAAATCACCGAAGAAGCCAC
AcAREB5	ATGGCTTCTTCGGTGATTTA	CTGCTGCTGTGATGATCTAAC
GUS	CACAAACCGTTCTACTTTACTGG	GCGTAAGGGTAATGCGAGGT
AcActin	TGGAATGGAAGCTGCAGGA	CACCACTGAGCACAATGTTGC

引物名称 Primer name	引物序列（5′-3′） Primer sequence
ProAcGH3.1-pHIS2	F:GGGCGAATTCCCGGGGAGCTCCTTTTATATATATATATATAT
	R:CGGATCGATTCGCGAACGCGTATATGAAGTGGTCCCCAATT
AcAREB1-BD	F:TCAGAGGAGGACCTGCATATGATGAACTTCAAGAACTATGG
	R:CCGCTGCAGGTCGACGGATCCTTAGCTCCTTGCTGCGTGTT
AcAREB1-AD	F:CGTACCAGATTACGCTCATATGATGAACTTCAAGAACTATGG R:GCAGCTCGAGCTCGATGGATCCTTAGCTCCTTGCTGCGTGTT
AcAREB2-AD	F:CGTACCAGATTACGCTCATATGATGGGATCATATTTGAACTT R:GCAGCTCGAGCTCGATGGATCCTTATTCATTGCATAAAATAG
AcAREB3-AD	F:CGTACCAGATTACGCTCATATGATGTACTTCAAGAACTATGG R:GCAGCTCGAGCTCGATGGATCCTCACCATGGGCTAGTCTGTG
AcAREB4-AD	F:CGTACCAGATTACGCTCATATGATGAACTTCAGAAACTTTGG R:GCAGCTCGAGCTCGATGGATCCTCATGTTTCAACTATTGAAT
AcAREB5-AD	F:CGTACCAGATTACGCTCATATGATGAACTTCAGAAACTTTGA R:GCAGCTCGAGCTCGATGGATCCTCACCATGGGCCAGTTTGTG
AcAREB1-GFP	F:CCAAATCGACTCTAGTCTAGAATGAACTTCAAGAACTATGG R:GCCCTTGCTCACCATGGTACCGCTCCTTGCTGCGTGTT
ProAcGH3.1-GUS	F:AACTGCAGCTTTTATATATATATATATAT R:CGGGATCCATATGAAGTGGTCCCCAATT

引物名称 Primer name	引物序列（5′-3′） Primer sequence
ProAcGH3.1-pHIS2	F:GGGCGAATTCCCGGGGAGCTCCTTTTATATATATATATATAT
	R:CGGATCGATTCGCGAACGCGTATATGAAGTGGTCCCCAATT
AcAREB1-BD	F:TCAGAGGAGGACCTGCATATGATGAACTTCAAGAACTATGG
	R:CCGCTGCAGGTCGACGGATCCTTAGCTCCTTGCTGCGTGTT
AcAREB1-AD	F:CGTACCAGATTACGCTCATATGATGAACTTCAAGAACTATGG R:GCAGCTCGAGCTCGATGGATCCTTAGCTCCTTGCTGCGTGTT
AcAREB2-AD	F:CGTACCAGATTACGCTCATATGATGGGATCATATTTGAACTT R:GCAGCTCGAGCTCGATGGATCCTTATTCATTGCATAAAATAG
AcAREB3-AD	F:CGTACCAGATTACGCTCATATGATGTACTTCAAGAACTATGG R:GCAGCTCGAGCTCGATGGATCCTCACCATGGGCTAGTCTGTG
AcAREB4-AD	F:CGTACCAGATTACGCTCATATGATGAACTTCAGAAACTTTGG R:GCAGCTCGAGCTCGATGGATCCTCATGTTTCAACTATTGAAT
AcAREB5-AD	F:CGTACCAGATTACGCTCATATGATGAACTTCAGAAACTTTGA R:GCAGCTCGAGCTCGATGGATCCTCACCATGGGCCAGTTTGTG
AcAREB1-GFP	F:CCAAATCGACTCTAGTCTAGAATGAACTTCAAGAACTATGG R:GCCCTTGCTCACCATGGTACCGCTCCTTGCTGCGTGTT
ProAcGH3.1-GUS	F:AACTGCAGCTTTTATATATATATATATAT R:CGGGATCCATATGAAGTGGTCCCCAATT

名称 Name	基因编号 Gene ID	染色体定位 Chromosome No.	蛋白长度/aa Protein length	分子量/kD Molecular weight	等电点 Isoelectric point	亚细胞定位 Subcellular localization
AcAREB1	Ach00g131571	未知 Unknow	431	47.30	9.79	细胞核 Nuclear
AcAREB2	Ach06g233651	06	448	49.08	9.16	细胞核 Nuclear
AcAREB3	Ach00g227711	未知 Unknow	411	45.04	10.10	细胞核 Nuclear
AcAREB4	Ach11g026271	11	459	49.43	5.40	细胞核 Nuclear
AcAREB5	Ach05g315671	05	396	42.63	7.51	细胞核 Nuclear

Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit

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