Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii）

doi:10.16420/j.issn.0513-353x.2021-0927

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 27-35.doi: 10.16420/j.issn.0513-353x.2021-0927

• Research Papers • Previous Articles Next Articles

Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii）

JIANG Jingdong¹, WEI Zhuangmin², WANG Nan¹, ZHU Chenqiao¹, YE Junli¹, XIE Zongzhou¹^,^*(), DENG Xiuxin¹, CHAI Lijun¹^,^*()

¹National Key for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops，College of Horticulture and Forestry Science，Huazhong Agricultural University，Wuhan 430070，China
²Guangxi Subtropical Crops Research Institute，Nanning 530001，China

Received:2022-04-27 Revised:2022-08-03 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：chailijun@mail.hzau.edu.cn，xiezz@webmail.hzau.edu.cn）

Abstract

Abstract:

A tetraploid plant was discovered in the native population of Hongkong kumquat （Fortunella hindsii）by flow cytometry. The leaf，flower，pollen，fruit，seed，and other characters of this tetraploid Hongkong kumquat were identified with the diploid Hongkong kumquat as control，and new tetraploid material was created by self-pollination of this tetraploid Hongkong kumquat. The results showed that compared with diploid Hongkong kumquat，the leaves of tetraploid are wider and thicker，leaf shape index becomes smaller，the stomatal density of the leaves becomes smaller and the stomatal apparatus is larger. The flowering diameter，whole flower length，petal length，filament length，pistil length，and pollen grain size of tetraploid are significantly larger than diploid，and the flower outer wall decoration density is significantly less than diploid，the fruit shape index of the tetraploid is larger，the peel thickness increases and the color index is not significantly different，the transverse diameter of tetraploid seeds is significantly different from diploid，the longitudinal diameter is significantly larger than diploid，and itsseed embryo type is polyembryonic. The progenies of tetraploid Hongkong kumquat are tetraploid. All the inbred progenies of tetraploid Hongkong kumquat are tetraploid. In this study，tetraploid Hongkong kumquat was discovered for the first time，its morphological indexes were identified. A new tetraploid was established in utilizing this material，which provided valuable materials for future citrus breeding and basic research.

Key words: citrus, flow cytometry, polyploidy creation, identification

CLC Number:

S666

JIANG Jingdong, WEI Zhuangmin, WANG Nan, ZHU Chenqiao, YE Junli, XIE Zongzhou, DENG Xiuxin, CHAI Lijun. Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii）[J]. Acta Horticulturae Sinica, 2023, 50(1): 27-35.

Figures/Tables 8

References 30

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材料 Material	长度/mm Length	宽度/mm Width	叶形指数 Leaf index	单叶质量/g Leaf weight	厚度/mm Thickness	面积/mm² Leaf area
2x	59.36 ± 4.14	20.33 ± 2.18	2.93 ± 0.21	0.24 ± 0.05	0.42 ± 0.05	796.12 ± 169.76
4x	63.27 ± 4.14	26.38 ± 1.96^**	2.40 ± 0.14^**	0.35 ± 0.04^**	0.58 ± 0.07^**	1091.81 ± 131.25^**
材料 Material	气孔Leaf stomata
材料 Material	数量 Number	长度/mm Length	宽度/mm Width
2x	72.20 ± 1.60	20.66 ± 0.60	19.12 ± 0.41
4x	23.40 ± 2.58^**	30.68 ± 0.57^**	27.05 ± 0.82^**

材料 Material	长度/mm Length	宽度/mm Width	叶形指数 Leaf index	单叶质量/g Leaf weight	厚度/mm Thickness	面积/mm² Leaf area
2x	59.36 ± 4.14	20.33 ± 2.18	2.93 ± 0.21	0.24 ± 0.05	0.42 ± 0.05	796.12 ± 169.76
4x	63.27 ± 4.14	26.38 ± 1.96^**	2.40 ± 0.14^**	0.35 ± 0.04^**	0.58 ± 0.07^**	1091.81 ± 131.25^**
材料 Material	气孔Leaf stomata
材料 Material	数量 Number	长度/mm Length	宽度/mm Width
2x	72.20 ± 1.60	20.66 ± 0.60	19.12 ± 0.41
4x	23.40 ± 2.58^**	30.68 ± 0.57^**	27.05 ± 0.82^**

材料 Material	开花直径/mm Diameter	整花长度/mm Flower length	花瓣厚度/mm Petal thichness	花瓣长度/mm Petal length	雌蕊长度/mm Pistil length	花丝长度/mm Filament length
2x	3.50 ± 0.43	6.48 ± 0.25	0.26 ± 0.03	5.74 ± 0.41	2.19 ± 0.24	2.92 ± 0.28
4x	5.04 ± 0.26^**	9.04 ± 0.72^**	0.72 ± 0.12^**	7.13 ± 0.55^**	2.84 ± 0.13^**	3.74 ± 0.14^**
材料 Material	花粉粒 Pollen grain
材料 Material	极轴长/μm Pollen polar axis length		赤道轴长/μm Pollen equatorial axis length		花粉外壁纹饰数量 Number of pollen exine ornamentation
2x	20.76 ± 0.47		19.46 ± 0.69		34.30 ± 2.83
4x	28.09 ± 0.45^**		25.44 ± 0.66^**		23.60 ± 2.24^**

材料 Material	开花直径/mm Diameter	整花长度/mm Flower length	花瓣厚度/mm Petal thichness	花瓣长度/mm Petal length	雌蕊长度/mm Pistil length	花丝长度/mm Filament length
2x	3.50 ± 0.43	6.48 ± 0.25	0.26 ± 0.03	5.74 ± 0.41	2.19 ± 0.24	2.92 ± 0.28
4x	5.04 ± 0.26^**	9.04 ± 0.72^**	0.72 ± 0.12^**	7.13 ± 0.55^**	2.84 ± 0.13^**	3.74 ± 0.14^**
材料 Material	花粉粒 Pollen grain
材料 Material	极轴长/μm Pollen polar axis length		赤道轴长/μm Pollen equatorial axis length		花粉外壁纹饰数量 Number of pollen exine ornamentation
2x	20.76 ± 0.47		19.46 ± 0.69		34.30 ± 2.83
4x	28.09 ± 0.45^**		25.44 ± 0.66^**		23.60 ± 2.24^**

材料 Material	果实横径/mm Horizontal diameter of fruit	果实纵径/mm Longitudinal diameter of fruit	果形指数 Fruit shape index	果皮厚度/mm Peel thickness	色泽指数 Color index	种子横径/mm Horizontal diameter of seed	种子纵径/mm Longitudinal diameter of seed
2x	9.79 ± 1.29	10.95 ± 1.00	1.23 ± 0.10	0.55 ± 0.05	13.31 ± 1.30	5.00 ± 0.35	6.10 ± 0.50
4x	7.98 ± 0.55^**	12.00 ± 1.66^*	1.51 ± 0.14^**	0.67 ± 0.12^**	13.25 ± 1.30^*	4.66 ± 0.33^*	7.73 ± 0.43^**

Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii）

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