Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 15-26.doi: 10.16420/j.issn.0513-353x.2021-0935
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TANG Yuqing, YANG Huidong, YAN Chengpu, WANG Siyu, WANG Yuting, HU Zhongdong*(), ZHU Fanghong*(
)
Received:
2022-08-25
Revised:
2022-12-02
Online:
2023-01-25
Published:
2023-01-18
Contact:
*(E-mail:CLC Number:
TANG Yuqing, YANG Huidong, YAN Chengpu, WANG Siyu, WANG Yuting, HU Zhongdong, ZHU Fanghong. Development and Application of Jinlan Pummelo(Citrus maxima)InDel Markers Based on Genome Re-sequencing[J]. Acta Horticulturae Sinica, 2023, 50(1): 15-26.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0935
序号 No. | 品种名 Name | 采集地 Place | 序号 No. | 品种名 Name | 采集地 Place |
---|---|---|---|---|---|
1 | 金堂绿柚 Jintang Lüyou | 重庆Chongqing | 25 | 桃溪蜜柚Taoxi Miyou | 江西Jiangxi |
2 | 北碚柚Beibeiyou | 重庆Chongqing | 26 | 金沙柚Jinshayou | 江西Jiangxi |
3 | 四季抛Sijipao | 重庆Chongqing | 27 | 金兰柚Jinlanyou | 江西Jiangxi |
4 | 梁沙柚Liangshayou | 重庆Chongqing | 28 | 凤凰柚Fenghuangyou | 江西Jiangxi |
5 | 暹罗柚Siamese Pummelo | 重庆Chongqing | 29 | 永嘉早香柚Yongjia Zaoxiangyou | 江西Jiangxi |
6 | 橘红彩柚Juhong Caiyou | 重庆Chongqing | 30 | 奥兰柚Oroblanco Grapefruit | 广西Guangxi |
7 | 彭县漳州柚Pengxian Zhangzhouyou | 重庆Chongqing | 31 | 橙柚Chengyou | 广西Guangxi |
8 | 纳西樱桃柚Naxi Yingtaoyou | 重庆Chongqing | 32 | 西施柚Xishiyou | 广西Guangxi |
9 | 安江石榴柚Anjiang Shiliuyou | 重庆Chongqing | 33 | 沙田柚Shatianyou | 广西Guangxi |
10 | 暹沙柚Xianshayou | 重庆Chongqing | 34 | 泰国晚金柚Thailand Wanjinyou | 广西Guangxi |
11 | 永安柚Yonganyou | 湖南Hunan | 35 | 云南脆香甜Yunnan Cuixiangtian | 广西Guangxi |
12 | 金香柚Jinxiangyou | 湖南Hunan | 36 | 泰国早2号Taiguozao 2 | 广西Guangxi |
13 | 文旦柚Wendanyou | 湖南Hunan | 37 | 西双版纳1号Xishuangbanna 1 | 广西Guangxi |
14 | 安江香柚Anjiang Xiangyou | 湖南Hunan | 38 | 鸡尾葡萄柚Cocktail Grapefruit | 福建Fujian |
15 | 冰糖柚Bingtangyou | 湖南Hunan | 39 | 星露比葡萄柚Star Ruby Grapefruit | 福建Fujian |
16 | 黔阳红心柚Qianyang Hongxinyou | 湖南Hunan | 40 | 麻步文旦柚Mabu Wendanyou | 福建Fujian |
17 | 左氏柚Zuoshiyou | 湖南Hunan | 41 | 泰国金丝柚Thailand Jinsiyou | 福建Fujian |
18 | 安江红心柚Anjiang Hongxinyou | 湖南Hunan | 42 | 尤溪橘柚Youxi Juyou | 福建Fujian |
19 | 土柚Tuyou | 湖南Hunan | 43 | 白肉琯溪蜜柚Bairou Guanximiyou | 福建Fujian |
20 | 缅甸柚Myanmar Pummelo | 湖南Hunan | 44 | 红肉琯溪蜜柚 Hongrou Guanximiyuo | 福建Fujian |
21 | 安江冰糖柚10号 Anjiang Bingtangyou 10 | 湖南Hunan | 45 | 可口柚Kekouyou | 广东Guangdong |
22 | 华农红柚Huanong Hongyou | 湖北Hubei | 46 | 翡翠柚Feicuiyou | 广东Guangdong |
23 | 秭归蜜柚1号Zigui Miyou1 | 湖北Hubei | 47 | 坪山柚Pingshanyou | 广东Guangdong |
24 | 秭归蜜柚2号Zigui Miyou2 | 湖北Hubei | 48 | 火焰葡萄柚Flame Grapefruit | 广东Guangdong |
Table 1 The information of 48 pomelo varieties
序号 No. | 品种名 Name | 采集地 Place | 序号 No. | 品种名 Name | 采集地 Place |
---|---|---|---|---|---|
1 | 金堂绿柚 Jintang Lüyou | 重庆Chongqing | 25 | 桃溪蜜柚Taoxi Miyou | 江西Jiangxi |
2 | 北碚柚Beibeiyou | 重庆Chongqing | 26 | 金沙柚Jinshayou | 江西Jiangxi |
3 | 四季抛Sijipao | 重庆Chongqing | 27 | 金兰柚Jinlanyou | 江西Jiangxi |
4 | 梁沙柚Liangshayou | 重庆Chongqing | 28 | 凤凰柚Fenghuangyou | 江西Jiangxi |
5 | 暹罗柚Siamese Pummelo | 重庆Chongqing | 29 | 永嘉早香柚Yongjia Zaoxiangyou | 江西Jiangxi |
6 | 橘红彩柚Juhong Caiyou | 重庆Chongqing | 30 | 奥兰柚Oroblanco Grapefruit | 广西Guangxi |
7 | 彭县漳州柚Pengxian Zhangzhouyou | 重庆Chongqing | 31 | 橙柚Chengyou | 广西Guangxi |
8 | 纳西樱桃柚Naxi Yingtaoyou | 重庆Chongqing | 32 | 西施柚Xishiyou | 广西Guangxi |
9 | 安江石榴柚Anjiang Shiliuyou | 重庆Chongqing | 33 | 沙田柚Shatianyou | 广西Guangxi |
10 | 暹沙柚Xianshayou | 重庆Chongqing | 34 | 泰国晚金柚Thailand Wanjinyou | 广西Guangxi |
11 | 永安柚Yonganyou | 湖南Hunan | 35 | 云南脆香甜Yunnan Cuixiangtian | 广西Guangxi |
12 | 金香柚Jinxiangyou | 湖南Hunan | 36 | 泰国早2号Taiguozao 2 | 广西Guangxi |
13 | 文旦柚Wendanyou | 湖南Hunan | 37 | 西双版纳1号Xishuangbanna 1 | 广西Guangxi |
14 | 安江香柚Anjiang Xiangyou | 湖南Hunan | 38 | 鸡尾葡萄柚Cocktail Grapefruit | 福建Fujian |
15 | 冰糖柚Bingtangyou | 湖南Hunan | 39 | 星露比葡萄柚Star Ruby Grapefruit | 福建Fujian |
16 | 黔阳红心柚Qianyang Hongxinyou | 湖南Hunan | 40 | 麻步文旦柚Mabu Wendanyou | 福建Fujian |
17 | 左氏柚Zuoshiyou | 湖南Hunan | 41 | 泰国金丝柚Thailand Jinsiyou | 福建Fujian |
18 | 安江红心柚Anjiang Hongxinyou | 湖南Hunan | 42 | 尤溪橘柚Youxi Juyou | 福建Fujian |
19 | 土柚Tuyou | 湖南Hunan | 43 | 白肉琯溪蜜柚Bairou Guanximiyou | 福建Fujian |
20 | 缅甸柚Myanmar Pummelo | 湖南Hunan | 44 | 红肉琯溪蜜柚 Hongrou Guanximiyuo | 福建Fujian |
21 | 安江冰糖柚10号 Anjiang Bingtangyou 10 | 湖南Hunan | 45 | 可口柚Kekouyou | 广东Guangdong |
22 | 华农红柚Huanong Hongyou | 湖北Hubei | 46 | 翡翠柚Feicuiyou | 广东Guangdong |
23 | 秭归蜜柚1号Zigui Miyou1 | 湖北Hubei | 47 | 坪山柚Pingshanyou | 广东Guangdong |
24 | 秭归蜜柚2号Zigui Miyou2 | 湖北Hubei | 48 | 火焰葡萄柚Flame Grapefruit | 广东Guangdong |
区域 Region | 标记数量(比例) Number of markers(Percentage) | 区域 Region | 标记数量(比例) Number of markers(Percentage) |
---|---|---|---|
外显子 Exonic | 7 311(1.67%) | 内含子 Intronic | 74 432(16.96%) |
基因上游1 kb区域 Upstream | 97 700(22.27%) | 基因下游1 kb区域 Downstream | 68 129(15.53%) |
3'UTR | 7 825(1.78%) | 5'UTR | 5 695(1.30%) |
基因上游1 kb区域,同时也在另一基因下游1 kb区域 Up-and downstream | 86 720(19.77%) | 基因间区 Intergenic | 90 938(20.73%) |
Table 2 Number and distribution of Indel in different regions of Jinlanyou
区域 Region | 标记数量(比例) Number of markers(Percentage) | 区域 Region | 标记数量(比例) Number of markers(Percentage) |
---|---|---|---|
外显子 Exonic | 7 311(1.67%) | 内含子 Intronic | 74 432(16.96%) |
基因上游1 kb区域 Upstream | 97 700(22.27%) | 基因下游1 kb区域 Downstream | 68 129(15.53%) |
3'UTR | 7 825(1.78%) | 5'UTR | 5 695(1.30%) |
基因上游1 kb区域,同时也在另一基因下游1 kb区域 Up-and downstream | 86 720(19.77%) | 基因间区 Intergenic | 90 938(20.73%) |
引物 Primer | 正向引物(5′-3′) Forward primer | 反向引物(5′-3′) Reverse primer |
---|---|---|
M4 | TAGTGGCAACCGTGTGACTG | GAAGTTCCTTTTGTCTTTGTGGA |
M6 | TGCATGCATGTAGACAGATGTAG | CATCTTGTCGATGATCTGGTG |
M8 | AAATCTTGTTGTTAGTCTAATGTGTTG | AACTAAATCTTCTCTTTGACCATCC |
M12 | GGCCACTCGAGTTGGAATAA | AGGAGTGGCCATGTGGATAG |
M19 | GCCAAACGGGTCTTTAATTG | CATGAACTTTTGTTGGCGACT |
M26 | TGGAACCCAATTGATATGTGTC | TTATTCCCTTTTGCCGTCAG |
M29 | TTTTTCGTCTTCTGCAAGTATTGA | TAAGTGATAATGGGCACAGTTCTT |
M32 | GTGTTCATTGTCAGCGTTATACA | TTTTCGAGGGACAATGTCGT |
M38 | GTGGGATTATTGAGACCCACT | GGACTGCTGGGAATTTGGTA |
M40 | ACTGGTGCTCCCACATTAGG | ATTTGCAACCGTATGGGAAC |
M47 | CCACATGGCGATATAACATGA | CATCGCCCTTCTGATTCCT |
M51 | TGTTCTGACCATATTTGACCAAC | TGGGAGGGCATAAGAGAGTG |
M62 | TCCGTCGTCAATGACTTCAA | TGCTGCAGTTTTCTTTTTCAA |
M63 | AATCTGAGTTTTCCGAAACTTTTA | TTCGAATGTAGATGGAGGTCAA |
M65 | TGTCCGCAACTTTTAAGACG | TGAATAGGGTGGCTTGCTTT |
M67 | CACAGCGACATGCTTTCATT | GCTCCTAGTGACGTGATGCTT |
M69 | ATCCGAGCATTCAATTTTGTTT | CGGAGGGCAATTTGAATTTT |
M72 | CGGCATAAACTCTTGCAACTT | TCACAGGAGCAATTATGTCGTC |
M73 | TTACCGAGAGCCCTCAAATG | CCATCCTTATCAAGGACATGAA |
M74 | GGTCAAAAGTACCCCTTCTCG | TGGCCAAACGAACTTGTATTC |
M84 | AGCTGCAGGTCGCAATATCT | GGTAAGGCGGTGTGTGAAGT |
M86 | GGCCACCATGTTTAAGGAAA | GCCATATTATTGTTGAGATACAACTTT |
M90 | GCGTGAGCTTGTATCCTTGC | CTCGGTCCTCTGGATTTTCA |
M93 | CAGAGTTGCGAATTTATCAGAAAG | GCGTTACAACACAAAGCTTGA |
Table 3 primer sequence of 24 pairs of InDel markers
引物 Primer | 正向引物(5′-3′) Forward primer | 反向引物(5′-3′) Reverse primer |
---|---|---|
M4 | TAGTGGCAACCGTGTGACTG | GAAGTTCCTTTTGTCTTTGTGGA |
M6 | TGCATGCATGTAGACAGATGTAG | CATCTTGTCGATGATCTGGTG |
M8 | AAATCTTGTTGTTAGTCTAATGTGTTG | AACTAAATCTTCTCTTTGACCATCC |
M12 | GGCCACTCGAGTTGGAATAA | AGGAGTGGCCATGTGGATAG |
M19 | GCCAAACGGGTCTTTAATTG | CATGAACTTTTGTTGGCGACT |
M26 | TGGAACCCAATTGATATGTGTC | TTATTCCCTTTTGCCGTCAG |
M29 | TTTTTCGTCTTCTGCAAGTATTGA | TAAGTGATAATGGGCACAGTTCTT |
M32 | GTGTTCATTGTCAGCGTTATACA | TTTTCGAGGGACAATGTCGT |
M38 | GTGGGATTATTGAGACCCACT | GGACTGCTGGGAATTTGGTA |
M40 | ACTGGTGCTCCCACATTAGG | ATTTGCAACCGTATGGGAAC |
M47 | CCACATGGCGATATAACATGA | CATCGCCCTTCTGATTCCT |
M51 | TGTTCTGACCATATTTGACCAAC | TGGGAGGGCATAAGAGAGTG |
M62 | TCCGTCGTCAATGACTTCAA | TGCTGCAGTTTTCTTTTTCAA |
M63 | AATCTGAGTTTTCCGAAACTTTTA | TTCGAATGTAGATGGAGGTCAA |
M65 | TGTCCGCAACTTTTAAGACG | TGAATAGGGTGGCTTGCTTT |
M67 | CACAGCGACATGCTTTCATT | GCTCCTAGTGACGTGATGCTT |
M69 | ATCCGAGCATTCAATTTTGTTT | CGGAGGGCAATTTGAATTTT |
M72 | CGGCATAAACTCTTGCAACTT | TCACAGGAGCAATTATGTCGTC |
M73 | TTACCGAGAGCCCTCAAATG | CCATCCTTATCAAGGACATGAA |
M74 | GGTCAAAAGTACCCCTTCTCG | TGGCCAAACGAACTTGTATTC |
M84 | AGCTGCAGGTCGCAATATCT | GGTAAGGCGGTGTGTGAAGT |
M86 | GGCCACCATGTTTAAGGAAA | GCCATATTATTGTTGAGATACAACTTT |
M90 | GCGTGAGCTTGTATCCTTGC | CTCGGTCCTCTGGATTTTCA |
M93 | CAGAGTTGCGAATTTATCAGAAAG | GCGTTACAACACAAAGCTTGA |
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