Isolation of Phenotypic Traits from Wild Cordyceps militaris Fruitbody

doi:10.16420/j.issn.0513-353x.2021-0683

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2449-2454.doi: 10.16420/j.issn.0513-353x.2021-0683

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Isolation of Phenotypic Traits from Wild Cordyceps militaris Fruitbody

XIAO Jun, CHEN Xun, ZHAO Ying(), MA Xiaoying, GONG Na, LIU Guoli

Edible Fungi Research Institute，Liaoning Academy of Agricultural Sciences，Shenyang 110161，China

Received:2022-03-07 Revised:2022-04-20 Online:2022-11-25 Published:2022-11-25
Contact: ZHAO Ying E-mail:zhaoying_226@163.com

Abstract

Abstract:

Six Cordyceps militaris（C5-1，C5-2，D1-1，D1-2，D1-2，D5-1，D5-2）which were the offspring of three（C5，D1，D5）wild C. militaris which were collected from Xifeng and Dashiqiao of Liaoning Province were compared in terms of colony morphology，agronomic traits and nutritional composition. Then，the genetic differences were analyzed by ISSR molecular markers. The results showed that there were significant differences in growth rate，fruiting body height and density among the differentiated strains from the same wild cordyceps militaris fruiting body. It can be seen from the ISSR marker analysis of the differentiated strains that the genotypes of D1-1 and D1-2，D5-1 and D5-2 from the same wild cordyceps militaris were significantly different. Moreover，there were significant differences in the content of cordycepin and adenosine among the differentiated cordyceps militaris strains from the same wild cordyceps militaris strains.

Key words: Cordyceps militaris, phenotypic trait, cordycepin, adenosine, differentiated strain

CLC Number:

S646
Q 939.9

XIAO Jun, CHEN Xun, ZHAO Ying, MA Xiaoying, GONG Na, LIU Guoli. Isolation of Phenotypic Traits from Wild Cordyceps militaris Fruitbody[J]. Acta Horticulturae Sinica, 2022, 49(11): 2449-2454.

Figures/Tables 6

References 10

[1]	Dai Yucheng, Yang Zhuliang. 2008. The list of Medicinal fungi in China and the revision of some names. Mycosystema, 27:801-824. (in Chinese)
	戴玉成, 杨祝良. 2008. 中国药用真菌名录及部分名称的修订. 菌物学报, 27:801-824.
[2]	Dai Yucheng, Zhou Liwei, Yang Zhuliang, Wen Huaan, BAU Tolgor, Li Taihui. 2010. A revised checklist of edible fungi in China. Mycosystema, 29:1-2. (in Chinese)
	戴玉成, 周丽伟, 杨祝良, 文华安, 图力古尔, 李泰辉. 2010. 中国食用菌名录. 菌物学报, 29:1-2.
[3]	Jia Junqiang, Shen Jian. 2013. Enzymatic modification of Cordyceps militaris polysaccharide and its antioxidant activity. Food Science, 34 (1):114-120. (in Chinese)
	贾俊强, 沈健. 2013. 蛹虫草多糖的酶法修饰及其抗氧化活性. 食品科学, 34 (1):114-120.
[4]	Li Cuixin, Zhang Guoqing, He Yongzhen, Wang Hexiang. 2007. Isolation of wild Cordyceps militaris and screening of high-yield strains. Edible Fungi of China, 26 (2):20-21. (in Chinese)
	李翠新, 张国庆, 何永珍, 王贺祥. 2007. 野生蛹虫草的分离与高产菌株的筛选. 中国食用菌, 26 (2):20-21.
[5]	Lu Qun, Luo Shaohong. 2011. Inhibition of Cordyceps sinensis on human hepatocellular carcinoma Bel-7402 cells and its mechanism. Chinese Pharmacological Bulletin, 27 (10):1477-1478. (in Chinese)
	卢群, 罗少洪. 2011. 虫草素对人肝癌Bel-7402细胞抑制及作用机制的研究. 中国药理学通报, 27 (10):1477-1478.
[6]	Wang Qi. 2010. Research of anti-ageing efficacy and biological characteristics of Cordyceps militaris[M. D. Dissertation]. Wuhan:Huazhong Agricultural University:83-85. (in Chinese)
	王奇. 2010. 蛹虫草的生物学特性及抗衰老功效研究[硕士论文]. 武汉:华中农业大学:83-85.
[7]	Wang Rongyu. 2012. Analysis the germplasm resources of Cordyceps militaris by inter-simple sequence repeat and screening of Cordyceps militaris with high yield and cordyceps content[M. D. Dissertation]. Fuzhou:Fujian Agriculture and Forestry University:14-21. (in Chinese)
	王荣宇. 2012. 蛹虫草种质资源ISSR分析与优质高产菌株筛选[硕士论文]. 福州:福建农林大学:14-21.
[8]	Xiao Jun, Xia Shuang, Chen Xun, Zhao Ying. 2018. Isolation of wild Cordyceps militaris and preliminary study on its molecular identification of growing grass. Northern Horticulture,(12):169-173. (in Chinese)
	肖军, 夏爽, 陈珣, 肇莹. 2018. 野生蛹虫草的分离及出草分子检测研. 北方园艺,(12):169-173.
[9]	Xu Defeng, Sun Lijun, Wang Yaling. 2012. Current status and prospect of the research and development of Cordyceps militaris resource in antibacterial activity. Science and Technology of Food Industry, 8 (26):424-427. (in Chinese)
	徐德峰, 孙力军, 王雅玲. 2012. 蛹虫草抑菌活性研究开发现状与发展趋势. 食品工业科技, 8 (26):424-427.
[10]	Yan W J, Li T H, Lin Q Y, Song B, Jiang Z D. 2010. Safety assessment of Cordyeps guangdongensis. Food and Chemical Toxicology, 48 (11):3080-3084. doi: 10.1016/j.fct.2010.08.001 URL

引物编号 Primer	核苷酸序列（5′-3′） Sequence	引物编号 Primer	核苷酸序列（5′-3′） Sequence
ISSR1	CTCCTCCTCCTCSC	ISSR6	DHBCGACGACGACGACGA
ISSR2	BDBACAACAACAACAACA	ISSR7	AGAGAGAGAGAGAGAGT
ISSR3	CACACACACACACACAWG	ISSR8	GAGAGAGAGAGAGAGAC
ISSR4	CACCACCACCACSC	ISSR9	CTCTCTCTCTCTCTCTG
ISSR5	VDHTCGTCGTCGTCGTCG	ISSR10	CACACACACACACACAG

引物编号 Primer	核苷酸序列（5′-3′） Sequence	引物编号 Primer	核苷酸序列（5′-3′） Sequence
ISSR1	CTCCTCCTCCTCSC	ISSR6	DHBCGACGACGACGACGA
ISSR2	BDBACAACAACAACAACA	ISSR7	AGAGAGAGAGAGAGAGT
ISSR3	CACACACACACACACAWG	ISSR8	GAGAGAGAGAGAGAGAC
ISSR4	CACCACCACCACSC	ISSR9	CTCTCTCTCTCTCTCTG
ISSR5	VDHTCGTCGTCGTCGTCG	ISSR10	CACACACACACACACAG

分化株 Differentiated strain	暗培养7 d The first 7 days without light	满皿Full dish
分化株 Differentiated strain	暗培养7 d The first 7 days without light	+ 见光培养 + Light	+ 暗培养 + No light
C5-1	3.31 ± 0.08 ab	2.76 ± 0.22 a	3.17 ± 0.14 ab
C5-2	3.52 ± 0.14 b	3.34 ± 0.14 b	3.24 ± 0.08 ab
D1-1	3.29 ± 0.14 ab	3.12 ± 0.22 ab	3.01 ± 0.29 a
D1-2	3.18 ± 0.08 ab	3.21 ± 0.08 b	3.23 ± 0.08 ab
D5-1	3.93 ± 0.28 c	3.29 ± 0.15 b	3.46 ± 0.22 b
D5-2	3.11 ± 0.16 a	3.02 ± 0.16 ab	3.14 ± 0.16 ab

分化株 Differentiated strain	暗培养7 d The first 7 days without light	满皿Full dish
分化株 Differentiated strain	暗培养7 d The first 7 days without light	+ 见光培养 + Light	+ 暗培养 + No light
C5-1	3.31 ± 0.08 ab	2.76 ± 0.22 a	3.17 ± 0.14 ab
C5-2	3.52 ± 0.14 b	3.34 ± 0.14 b	3.24 ± 0.08 ab
D1-1	3.29 ± 0.14 ab	3.12 ± 0.22 ab	3.01 ± 0.29 a
D1-2	3.18 ± 0.08 ab	3.21 ± 0.08 b	3.23 ± 0.08 ab
D5-1	3.93 ± 0.28 c	3.29 ± 0.15 b	3.46 ± 0.22 b
D5-2	3.11 ± 0.16 a	3.02 ± 0.16 ab	3.14 ± 0.16 ab

分化株Differentiated strain	数量Number	高度/cm Height
C5-1	60.33 ± 2.94 a	5.32 ± 0.22 a
C5-2	102.92 ± 4.97 b	5.16 ± 0.14 a
D1-1	144.50 ± 4.55 d	5.62 ± 0.28 a
D1-2	118.33 ± 2.16 c	8.80 ± 0.22 c
D5-1	108.00 ± 2.83 b	7.52 ± 0.16 b
D5-2	187.27 ± 5.10 e	9.56 ± 0.29 d

Isolation of Phenotypic Traits from Wild Cordyceps militaris Fruitbody

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分化株Differentiated strain	虫草素/（mg · kg^-1）Cordycepin	腺苷/（mg · kg^-1）Adenosine
C5-1	5 688.1 ± 44.9 a	2 336.9 ± 42.6 d
C5-2	3 953.4 ± 34.1 c	4 587.5 ± 57.9 b
D1-1	3 652.1 ± 44.6 c	980.6 ± 26.7 e
D1-2	5 502.9 ± 71.4 a	6 818.4 ± 51.5 a
D5-1	3 903.1 ± 47.4 c	4 025.6 ± 45.4 c
D5-2	4 997.3 ± 75.9 b	492.8 ± 42.3 f

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