Identification and Analysis of the Type of Leaf Variegation of Primulina pungentisepala

doi:10.16420/j.issn.0513-353x.2021-1038

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2388-2394.doi: 10.16420/j.issn.0513-353x.2021-1038

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Identification and Analysis of the Type of Leaf Variegation of Primulina pungentisepala

LI Yueya, HE Dong, CHEN Jiancun, LI Bo, BAI Meng, LUO Le(), Zhang Qixiang

School of Landscape Architecture Beijing Forestry University，University Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding，National Engineering Research Center for Floriculture，Beijing Laboratory of Urban and Rural Ecological Environment，Engineering Research Center of Landscape Environment of Ministry of Education，Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education，Beijing 100083，China

Received:2022-06-02 Revised:2022-09-22 Online:2022-11-25 Published:2022-11-25
Contact: LUO Le E-mail:luolebjfu@163.com

Abstract

Abstract:

Several features of Primulina pungentisepala leaf to determine the type of leaf variegation were analyzed. These features include：the morphological characteristics of leaf epidermis，the optical characteristics of leaf adaxial epidermis，the anatomical structure of leaf，and chlorophyll content and chlorophyll fluorescence parameters（F_v/F_m） of leaf. The results indicated relatively stable leaf variegation，showing fishbone-like characteristics. The variegated areas presented two reflection modes：spot reflection（spotted pattern）and polygon reflection（polygonal pattern）. Boundaries between tissues were blurry in variegated areas. Conspicuous intercellular spaces existed between the epidermal and palisade cells，and among palisade cells. In non-variegated areas，intercellular spaces were not observed between palisade cells. In variegated areas，the stacking degree was low and chloroplasts were sparse，but profuse and dense osmiophilic were presented. On the contrary，the stacking degree of chloroplast was high with fewer osmiophilic droplets in non-variegated areas. Between the variegated and non-variegated areas，the chlorophyll a content did not differ significantly，while the chlorophyll b content and the total chlorophyll content differed remarkably. The chlorophyll fluorescence parameters（F_v/F_m）showed no significant differences between the variegated and non-variegated areas. Collectively，these results indicate that the formation of silver-white leaf variegation of P. pungentisepala is mainly related to the conspicuous intercellular spaces between the epidermal and palisade cells，and among palisade cells，but is also affected by the reduction of chlorophyll contents.

Key words: Primulina pungentisepala, leaf variegation, air-space type leaf variegation, chlorophyll type leaf variegation, intercellular space, organization structure

CLC Number:

S682.1

LI Yueya, HE Dong, CHEN Jiancun, LI Bo, BAI Meng, LUO Le, Zhang Qixiang. Identification and Analysis of the Type of Leaf Variegation of Primulina pungentisepala[J]. Acta Horticulturae Sinica, 2022, 49(11): 2388-2394.

Figures/Tables 4

Fig. 1 Primulina pungentisepala plant（A）and the optical properties of variegated and non-variegated areas of leaf adaxial surface（B）

Fig.2 Leaf structure of variegated（A-C）and non-variegated（D-F）areas of Primulina pungentisepala Ead：Adaxial epidermal cells;IS：Intercellular space;P：Palisade tissue;SP：Spongy tissue.

Fig. 3 Chloroplast ultra structure of variegated（A，B）and non-variegated（C，D）areas of Primulina pungentisepala GL：Grana lamellae;SL：Stroma lamella;S：Starch grain;O：Osmiophilic droplet.

Table 1 Chlorophyll content and fluorescence parameter（Fv/Fm) of variegated and non-variegated areas

叶片类型 Leaf	叶绿素a/（mg · g^-1） Chlorophyll a	叶绿素b/（mg · g^-1） Chlorophyll b	总叶绿/（mg · g^-1） Chlorophyll a + b	F_v/F_m
斑区Variegated	0.165 ± 0.013 a	0.073 ± 0.008 b	0.238 ± 0.019 b	0.731 ± 0.007 a
非斑区Non-variegated	0.255 ± 0.063 a	0.112 ± 0.014 a	0.367 ± 0.077 a	0.738 ± 0.001 a

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Identification and Analysis of the Type of Leaf Variegation of Primulina pungentisepala

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