Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

doi:10.16420/j.issn.0513-353x.2021-0811

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2357-2366.doi: 10.16420/j.issn.0513-353x.2021-0811

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Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

YE Guangji¹^,², ZHENG Zhenzhen¹, NA Tiancang¹^,², WANG Jian¹^,²^,^**()

¹Qinghai Academy of Agriculture and Forestry Sciences，Xining 810016，China
²Qinghai Provincial Key Laboratory of Potato Breeding，Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources，Qinghai University Key Laboratory of Qinghai-Tibet Plateau Biotechnology of the Ministry of Education，Provincial and Ministry Co-construction of the State Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry，Xining 810016，China

Received:2022-04-07 Revised:2022-08-17 Online:2022-11-25 Published:2022-11-25
Contact: WANG Jian E-mail:jianwang2197@163.com

Abstract

Abstract:

The content of SGAs（Steroidal glycoalkaloids）was evaluated in the leaf and peel tissue of 11 potato resources，the resources with relatively low level of SGAs were selected. The gene expression related to the SGAs biosynthesis was determined in Xiazhai 65，LZ111，Q072625，Longshu 7，Qingshu 9，and Qingshu 11，which would help confirm the key genes in SGAs biosynthesis of potato and provide the potential to create the low SGAs potato resources in the future. These results showed that SGAs content in the leaves of 11 cultivars was 0.08600-1.58066 mg · g^-1 FW;the SGAs content in the peel tissue of potato tubers was 0.01415-0.18450 mg · g^-1 FW and lower than 0.20 mg · g^-1 FW（the safety level）. The genes of StSQS1，StCAS，StSSR2 and StGAME4 played key roles in SGAs biosynthesis.

Key words: Solanum tuberosum, steroidal glycoalkaloids, expression of gene

CLC Number:

S532

YE Guangji, ZHENG Zhenzhen, NA Tiancang, WANG Jian. Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources[J]. Acta Horticulturae Sinica, 2022, 49(11): 2357-2366.

Figures/Tables 5

References 36

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序号 Number	资源 Resource	块茎成熟期 Tuber mature period	薯皮色 Tuber skin colour	薯肉色 Tuber flesh colour
1	大西洋Daxiyang	早熟 Early maturity	白 White	白 White
2	青薯2号Qingshu 2	晚熟 Late maturity	白 White	白 White
3	青薯9号Qingshu 9	晚熟 Late maturity	红 Red	彩色 Colour
4	青薯10号Qingshu 10	晚熟 Late maturity	白 White	白 White
5	青薯11号Qingshu 11	晚熟 Late maturity	粉 Pink	彩色 Colour
6	陇薯6号Longshu 6	晚熟 Late maturity	白 White	白 White
7	陇薯7号Longshu 7	晚熟 Late maturity	黄 Yellow	黄 Yellow
8	下寨65 Xiazhai 65	中晚熟 Mid-late maturity	黄 Yellow	浅黄 Pale yellow
9	LZ111	晚熟 Late maturity	白 White	白 White
10	D0602-10	中晚熟 Mid-late maturity	白 White	白 White
11	Q072625	晚熟 Late maturity	白 White	白 White

序号 Number	资源 Resource	块茎成熟期 Tuber mature period	薯皮色 Tuber skin colour	薯肉色 Tuber flesh colour
1	大西洋Daxiyang	早熟 Early maturity	白 White	白 White
2	青薯2号Qingshu 2	晚熟 Late maturity	白 White	白 White
3	青薯9号Qingshu 9	晚熟 Late maturity	红 Red	彩色 Colour
4	青薯10号Qingshu 10	晚熟 Late maturity	白 White	白 White
5	青薯11号Qingshu 11	晚熟 Late maturity	粉 Pink	彩色 Colour
6	陇薯6号Longshu 6	晚熟 Late maturity	白 White	白 White
7	陇薯7号Longshu 7	晚熟 Late maturity	黄 Yellow	黄 Yellow
8	下寨65 Xiazhai 65	中晚熟 Mid-late maturity	黄 Yellow	浅黄 Pale yellow
9	LZ111	晚熟 Late maturity	白 White	白 White
10	D0602-10	中晚熟 Mid-late maturity	白 White	白 White
11	Q072625	晚熟 Late maturity	白 White	白 White

序号 Number	资源 Resource	α-茄碱/（mg · g^-1FW） α-Solanine	α-卡茄碱/（mg · g^-1FW） α-Chaconine	总SGA/（mg · g^-1FW） Total SGAs	α-卡茄碱/α-茄碱 α-Chaconine/α-Solanine
1	大西洋Daxiyang	0.01912 ± 0.00061 e	0.02213 ± 0.00075 f	0.04125 ± 0.00136 ef	1.16
2	青薯2号Qingshu 2	0.02625 ± 0.00089 d	0.04165 ± 0.00145 d	0.06790 ± 0.00234 d	1.59
3	青薯9号Qingshu 9	0.00653 ± 0.00007 i	0.00762 ± 0.00020 i	0.01415 ± 0.00027 i	1.17
4	青薯10号Qingshu 10	0.01608 ± 0.00007 f	0.02181 ± 0.00002 f	0.03789 ± 0.00005 f	1.36
5	青薯11号Qingshu 11	0.01233 ± 0.00005 g	0.01952 ± 0.00019 g	0.03185 ± 0.00015 g	1.58
6	陇薯6号Longshu 6	0.01814 ± 0.00024 e	0.02566 ± 0.00084 e	0.04380 ± 0.00107 e	1.41
7	陇薯7号Longshu 7	0.00975 ± 0.00018 h	0.01539 ± 0.00001 h	0.02514 ± 0.00018 h	1.58
8	下寨65 Xiazhai 65	0.06028 ± 0.00161 a	0.07129 ± 0.00209 b	0.13156 ± 0.00370 b	1.18
9	LZ111	0.05233 ± 0.00113 c	0.04757 ± 0.00145 c	0.09990 ± 0.00258 c	0.91
10	D0602-10	0.05528 ± 0.00145 b	0.12922 ± 0.00331 a	0.18450 ± 0.00475 a	2.34
11	Q072625	0.01294 ± 0.00023 g	0.01960 ± 0.00001 g	0.03254 ± 0.00022 g	1.51

序号 Number	资源 Resource	α-茄碱/（mg · g^-1FW） α-Solanine	α-卡茄碱/（mg · g^-1FW） α-Chaconine	总SGA/（mg · g^-1FW） Total SGAs	α-卡茄碱/α-茄碱 α-Chaconine/α-Solanine
1	大西洋Daxiyang	0.01912 ± 0.00061 e	0.02213 ± 0.00075 f	0.04125 ± 0.00136 ef	1.16
2	青薯2号Qingshu 2	0.02625 ± 0.00089 d	0.04165 ± 0.00145 d	0.06790 ± 0.00234 d	1.59
3	青薯9号Qingshu 9	0.00653 ± 0.00007 i	0.00762 ± 0.00020 i	0.01415 ± 0.00027 i	1.17
4	青薯10号Qingshu 10	0.01608 ± 0.00007 f	0.02181 ± 0.00002 f	0.03789 ± 0.00005 f	1.36
5	青薯11号Qingshu 11	0.01233 ± 0.00005 g	0.01952 ± 0.00019 g	0.03185 ± 0.00015 g	1.58
6	陇薯6号Longshu 6	0.01814 ± 0.00024 e	0.02566 ± 0.00084 e	0.04380 ± 0.00107 e	1.41
7	陇薯7号Longshu 7	0.00975 ± 0.00018 h	0.01539 ± 0.00001 h	0.02514 ± 0.00018 h	1.58
8	下寨65 Xiazhai 65	0.06028 ± 0.00161 a	0.07129 ± 0.00209 b	0.13156 ± 0.00370 b	1.18
9	LZ111	0.05233 ± 0.00113 c	0.04757 ± 0.00145 c	0.09990 ± 0.00258 c	0.91
10	D0602-10	0.05528 ± 0.00145 b	0.12922 ± 0.00331 a	0.18450 ± 0.00475 a	2.34
11	Q072625	0.01294 ± 0.00023 g	0.01960 ± 0.00001 g	0.03254 ± 0.00022 g	1.51

序号 Number	资源 Resource	α-茄碱/（mg · g^-1FW） α-Solanine	α-卡茄碱/（mg · g^-1FW） α-Chaconine	总SGA/（mg · g^-1FW） Total SGAs	α-卡茄碱/α-茄碱 α-Chaconine/α-Solanine
1	大西洋Daxiyang	0.56977 ± 0.00268 a	0.71562 ± 0.00140 d	1.28539 ± 0.00408 c	1.26
2	青薯2号Qingshu 2	0.40000 ± 0.00227 e	0.97333 ± 0.00538 b	1.37333 ± 0.00320 b	2.43
3	青薯9号Qingshu 9	0.01667 ± 0.00026 k	0.06933 ± 0.00097 i	0.08600 ± 0.00124 k	4.16
4	青薯10号Qingshu 10	0.43467 ± 0.01179 c	0.76667 ± 0.00586 c	1.20134 ± 0.01747 d	1.76
5	青薯11号Qingshu 11	0.18133 ± 0.00759 h	0.22867 ± 0.00993 h	0.41000 ± 0.01752 i	1.26
6	陇薯6号Longshu 6	0.41533 ± 0.00401 d	0.72667± 0.01983 d	1.14200 ± 0.02383 e	1.75
7	陇薯7号Longshu 7	0.50733 ± 0.01111 b	1.07333 ± 0.04750 a	1.58066 ± 0.05861 a	2.12
8	下寨65 Xiazhai 65	0.13733 ± 0.00087 i	0.37667 ± 0.00741 g	0.51400 ± 0.00828 h	2.74
9	LZ111	0.35533 ± 0.00090 f	0.64133 ± 0.02547 e	0.99666 ± 0.02637 f	1.80
10	D0602-10	0.23867 ± 0.00087 g	0.44933 ± 0.00639 f	0.68800 ± 0.00726 g	1.88
11	Q072625	0.06307 ± 0.00110 j	0.20467 ± 0.00479 h	0.26774 ± 0.00369 j	3.25

Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

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[1]	GUO Haixia1，ZHANG Jingjing2，AN Ran1，QIAO Yan1，SHI Wenhui1，SHI Jing1，and ZHANG Jinwen1,*. Regulation of SGAs Biosynthesis of Green Tissues in Aerial Part of the Potato [J]. ACTA HORTICULTURAE SINICA, 2017, 44(6): 1105-1115.
[2]	Yang Jinghui;Liu Yanjun;Luo Yunbo;Zhang Weiyu;Yang Enqin;Liu Yudong. The Stress-related Responses of Tomato and Expression of Nr Gene Caused by Ethephon and Abscisic Acid [J]. ACTA HORTICULTURAE SINICA, 2004, 31(4): 514-516.