The Neutral Interaction of Co-infection Papaya Between Papaya Ringspot Virus and Papaya Leaf Distortion Mosaic Virus

doi:10.16420/j.issn.0513-353x.2021-0804

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2336-2346.doi: 10.16420/j.issn.0513-353x.2021-0804

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The Neutral Interaction of Co-infection Papaya Between Papaya Ringspot Virus and Papaya Leaf Distortion Mosaic Virus

MO Cuiping¹, WU Zilin², GUO Yongyi¹, ZHANG Shuguang¹, LI Pengfei¹, LI Huaping¹^,^*()

¹Guangdong Province Key Laboratory of Microbial Signals and Disease Control，College of Plant Protection，South China Agricultural University，Guangzhou 510642，China
²Guangdong Sugarcane Genetic Improvement Engineering Center，Institute of Nanfan & Seed Industry，Guangdong Academy of Sciences，Guangzhou 510316，China

Received:2022-04-22 Revised:2022-07-15 Online:2022-11-25 Published:2022-11-25
Contact: LI Huaping E-mail:huaping@scau.edu.cn

Abstract

Abstract:

The commercialized transgenic papaya‘Huanong 1’has been utilized to successfully control the destructive virus-papaya ringspot virus（PRSV）in South China. However，another new emerging virus，papaya leaf distortion mosaic virus（PLDMV）was found in some PRSV-resistant transgenic and other non-transgenic plants in Guangdong and Hainan Provinces through a field investigation. The survey results indicated that the two viruses had co-infections on some papaya plants. To clarify the interrelationship between the two viruses on papaya，the characteristics of both virus incidence，symptoms and cellular pathology，and virus accumulation on transgenic and non-transgenic papaya were analyzed by artificial inoculation. The results showed that the transgenic papaya‘Huanong 1’was still strong resistant to PRSV，but highly susceptible to PLDMV，and PLDMV could not help PRSV overcome its resistance to transgenic papaya. In addition，it was not observed significant symptomatic difference on the transgenic and non-transgenic papaya by the single- or co-infection of two viruses. The virions of the two viruses mostly existed in different mesophyll cells，which were not more seriously damaged with co-infection of two viruses compared with single viral infection. Moreover，the accumulation of PRSV gradually decreased until it was undetectable 15 days post inoculation on the transgenic papaya，while the accumulation of PLDMV gradually increased and remained at a higher level. On transgenic or non-transgenic papaya，the accumulation of PRSV virus was lower than that of PLDMV，but the two viruses co-existed for a long time and each maintained the corresponding viral contents on the non-transgenic papaya. These results indicate that transgenic papaya‘Huanong 1’is highly susceptible to PLDMV，and PRSV and PLDMV were shown as neutral with no synergistic infected in papaya plants.

Key words: Carica papaya, papaya ringspot virus, papaya leaf distortion mosaic virus, co-infection, interaction analysis

CLC Number:

S668.2

MO Cuiping, WU Zilin, GUO Yongyi, ZHANG Shuguang, LI Pengfei, LI Huaping. The Neutral Interaction of Co-infection Papaya Between Papaya Ringspot Virus and Papaya Leaf Distortion Mosaic Virus[J]. Acta Horticulturae Sinica, 2022, 49(11): 2336-2346.

Figures/Tables 6

Table 1 Primer for RT-PCR and RT-qPCR of papaya virus

病毒 Virus	引物 Name	引物序列 Primer sequence	大小/bp Size
PRSV	PRSV	F：CTTCAACAGGGCTTTCTTGC;R：GAATTACCAAGAACAATGTG	927
PRSV	PRSV-q1	F：GACATATCTGGTGTCTGGGTAATGATG;R：GCGGCATGTACCTCTCAGTT	128
PLDMV	PLDMV	F：TCCGCTCTTGATGCTGGCAAATCC;R：CGGTGAGGTTCCGTTGAATAC	652
PLDMV	PLDMV-q1	F：GGAAAACGCAAAGCCCACTT;R：CGGTGAGGTTCCGTTGAATAC	169
chy	chy	F：ATCTACAATCTTGCTAACCCTA;R：AGTCATCTTGAGAATAACCCAC	281
chy	Chy-q	F：CGGATCCTCCCATTTCCCTT; R：CCCACGGTGTAAAAATCAGCAG	151

Fig. 1 RT-PCR detection of PRSV and PLDMV from some infected samples of papaya plants M：DL2000 plus marker;chy：Internal reference gene of papaya.

Table 2 Statistical table of incidence of PRSV and PLDMV on‘Huanong 1’and‘Baxi’papaya %

处理 Treatment	华农1号Huanong 1		巴西Baxi
处理 Treatment	PRSV	PLDMV	PRSV	PLDMV
Mock（健康对照Healthy control）	0 b	0 b	0 b	0 b
PRSV	8.70 ±1.30 a	0 b	94.07 ± 3.23 a	0 b
PLDMV	0 b	94.07 ± 3.23 a	0 b	100 ± 0 a
PRSV + PLDMV	3.61 ± 1.95 b	96.28 ± 3.72 a	97.22 ± 2..78 a	98.61 ± 1.39 a
PRSV→PLDMV	0 b	96.76 ± 1.67 a	90.74 ± 9.26 a	100 ± 0 a
PLDMV→PRSV	1.85 ± 1.85 b	100 ± 0 a	100 ± 0 a	100 ± 0 a

Fig. 2 Symptomatic of papaya inoculated with PRSV and PLDMV under different treatments

Fig. 3 Changes of virus accumulation after inoculation with PRSV and PLDMV

Fig. 4 The cytopathology of mesophyll cell from papaya plants infected with PRSV or PLDMV and co-infected with both viruses at 60 dpi A：Mesophyll cells from healthy papaya at the same growth duration;B：Mesophyll cells from papaya infected with PRSV，chloroplast is arranged separately;C：Mesophyll cells from papaya infected with PLDMV，the shape of mesophyll cells is irregular，and they fill with the typical pinwheels and filamentous virus particles;D：Mesophyll cells from papaya co-infected with both viruses，chloroplast atrophy is not compact，the shape of cells are extruded irregular with a lot of pinwheels-like inclusions. M：Mitochondria;N：Nucleus;CH：Chloroplast;CW：Cell wall;ER：Endoplasmic reticulum;PW：Pinwheel;arrow：Filamentous virus particles.

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The Neutral Interaction of Co-infection Papaya Between Papaya Ringspot Virus and Papaya Leaf Distortion Mosaic Virus

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